FastQCFastQC Report
Fri 27 May 2016
SRR939398_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939398_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2433007
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185760.7634996528986558No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151030.6207544820051895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111550.45848614492272316No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50870.2090828345335628No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37120.15256840609172106No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31220.12831857861485807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124900.036.3369451
GTATCAA190700.031.267411
CCGCGTA350.00798572927.42183166-67
GCGTATC908.9794263E-426.3742711
CATTCCG5550.024.77659
ATCAACG241300.024.5659163
GTACATG160400.024.4471741
TCAACGC242700.024.3499984
TATCAAC246350.024.1423782
CAACGCA245900.024.0267185
AACGCAG251600.023.5766226
TACATGG167550.023.0884442
ACATGGG167900.022.1046663
GAGTACT150150.021.97548712-13
AGTACTT156200.021.51888812-13
CGCAGAG283650.020.8792348
ACGCAGA284400.020.8741917
GTATAGG8650.020.8554941
GTACTTT163300.020.16232914-15
GCAGAGT289400.019.9564699
Produced by FastQC (version 0.11.5)