##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939398_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2433007 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.955144806406228 31.0 31.0 31.0 30.0 34.0 2 31.197294952295657 31.0 31.0 34.0 30.0 34.0 3 31.19261391356457 31.0 31.0 34.0 28.0 34.0 4 35.1053691995132 35.0 35.0 37.0 33.0 37.0 5 34.694080617112895 35.0 35.0 37.0 32.0 37.0 6 34.762431016433574 35.0 35.0 37.0 32.0 37.0 7 34.60884617265795 35.0 35.0 37.0 30.0 37.0 8 34.505002246191644 35.0 35.0 37.0 30.0 37.0 9 35.9886079242682 37.0 35.0 39.0 30.0 39.0 10-11 35.80384828321497 37.0 35.0 39.0 30.0 39.0 12-13 35.79705278283211 37.0 35.0 39.0 30.0 39.0 14-15 36.59152337005196 38.0 35.5 40.0 30.5 41.0 16-17 36.52841812621172 38.0 35.0 40.0 30.0 41.0 18-19 36.60790412851257 38.0 35.0 40.0 30.0 41.0 20-21 36.52468735190651 38.0 35.0 40.0 30.0 41.0 22-23 36.35686498230379 38.0 34.5 40.0 30.0 41.0 24-25 36.205375488027784 38.0 34.0 40.0 29.0 41.0 26-27 35.73863515394736 38.0 34.0 40.0 27.5 41.0 28-29 35.54879517403772 38.0 34.0 40.0 27.0 41.0 30-31 35.232370889191856 37.5 34.0 40.0 27.0 41.0 32-33 34.933108494961175 37.0 33.0 40.0 26.5 41.0 34-35 35.128626222612596 38.0 33.0 40.0 27.0 41.0 36-37 34.923758747919756 38.0 33.0 40.0 25.5 41.0 38-39 34.7257217098019 38.0 33.0 40.0 25.0 41.0 40-41 34.5570670367985 37.5 33.0 40.0 25.0 41.0 42-43 34.35840299678546 37.0 33.0 40.0 24.5 41.0 44-45 33.91494352461789 37.0 33.0 40.0 23.5 41.0 46-47 33.42380478149056 37.0 32.0 40.0 21.5 41.0 48-49 32.978304624688704 37.0 31.5 40.0 18.5 41.0 50-51 32.746181782460965 37.0 31.0 40.0 17.5 41.0 52-53 32.656816441547434 37.0 31.0 40.0 17.5 41.0 54-55 32.35096754756563 36.5 31.0 39.0 14.5 40.5 56-57 31.89536815964771 36.0 30.5 39.0 11.0 40.0 58-59 31.47491643057336 35.5 29.5 39.0 9.0 40.0 60-61 31.137261627278505 35.0 29.0 39.0 8.0 40.0 62-63 31.019990900149487 35.0 29.0 38.5 8.0 40.0 64-65 30.615225110326442 35.0 28.0 38.0 6.5 40.0 66-67 29.813128363379143 34.0 27.0 38.0 2.0 40.0 68-69 26.50424022618924 31.0 18.5 36.0 2.0 39.0 70-71 25.268695075682068 30.5 13.5 36.0 2.0 39.0 72-73 26.246319883173374 31.0 19.5 35.5 2.0 38.5 74-75 26.95278887401475 31.5 22.5 35.0 2.0 38.0 76-77 26.559615940274732 31.5 23.5 34.5 2.0 37.0 78-79 27.618171258857867 32.5 26.0 35.0 2.0 37.0 80-81 27.602394074493006 33.0 26.0 35.0 2.0 37.0 82-83 27.268764742559313 32.5 26.0 35.0 2.0 36.5 84-85 26.888547792916338 32.0 25.5 35.0 2.0 36.0 86-87 26.44817051492248 32.0 24.5 35.0 2.0 36.0 88-89 25.968318833443554 32.0 23.5 34.0 2.0 35.0 90-91 25.37927346694851 31.0 20.5 34.0 2.0 35.0 92-93 24.84677499900329 31.0 19.0 34.0 2.0 35.0 94-95 24.047120497392733 31.0 12.5 34.0 2.0 35.0 96-97 23.406801542289028 31.0 4.5 34.0 2.0 35.0 98-99 22.59621324558458 30.0 2.0 34.0 2.0 35.0 100-101 21.199008469766014 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2747188841088466 1101 2 0.2155330480443567 1101 3 0.36785307733075 1101 4 0.3391923174418565 1101 5 0.4349706188062399 1101 6 0.282384143541087 1101 7 0.511279617090473 1101 8 0.6827705770121071 1101 9 0.721411384960021 1101 10-11 0.7089421257876936 1101 12-13 0.6839472303609924 1101 14-15 0.670186169766751 1101 16-17 0.8538188185679019 1101 18-19 0.6428681153438163 1101 20-21 0.7663192660017941 1101 22-23 0.8040286748459664 1101 24-25 0.7818083905330226 1101 26-27 0.9261129991240935 1101 28-29 0.8321189009680978 1101 30-31 0.6239500976651939 1101 32-33 0.9542613068541144 1101 34-35 0.9542619689554925 1101 36-37 1.1209658860236331 1101 38-39 1.254672406253455 1101 40-41 1.164537134894907 1101 42-43 0.8141419397502361 1101 44-45 1.0002760695397086 1101 46-47 0.8705613395308305 1101 48-49 1.038448246436289 1101 50-51 0.7203434952884393 1101 52-53 0.6974010526144596 1101 54-55 0.6685691747446185 1101 56-57 0.8151044214699397 1101 58-59 0.831881877045415 1101 60-61 0.9631616698133634 1101 62-63 0.5567665985462895 1101 64-65 0.5004424199605282 1101 66-67 0.31595116266244005 1101 68-69 -6.732635035493761 1101 70-71 -7.798301357460961 1101 72-73 -4.362332148410854 1101 74-75 -2.242342737076502 1101 76-77 0.4230376657262944 1101 78-79 0.7054131781406774 1101 80-81 0.5139093655094094 1101 82-83 0.280982099154528 1101 84-85 0.2075516259086001 1101 86-87 0.05208822481870712 1101 88-89 0.024330838627815154 1101 90-91 0.01051171705182341 1101 92-93 0.07505321538156906 1101 94-95 0.19822628588268998 1101 96-97 0.07491640096853658 1101 98-99 -0.10669196755489807 1101 100-101 -0.15032654792320344 1102 1 0.3541044845028516 1102 2 0.2895087921881654 1102 3 0.3624124395371737 1102 4 0.24612432101251613 1102 5 0.42104197042380775 1102 6 0.3383911617329787 1102 7 0.46570754210663523 1102 8 0.6702655750113067 1102 9 0.6236030308491465 1102 10-11 0.6900845827704885 1102 12-13 0.6013103294467825 1102 14-15 0.7606492780870013 1102 16-17 0.676921136418116 1102 18-19 0.5629438379405514 1102 20-21 0.6401649581247995 1102 22-23 0.734077617499949 1102 24-25 0.7408496993642544 1102 26-27 0.5948805061268985 1102 28-29 0.5389477863684107 1102 30-31 0.514113855476019 1102 32-33 0.6975355396241483 1102 34-35 0.7138196381770285 1102 36-37 0.8043546261350656 1102 38-39 0.7967507760629147 1102 40-41 0.616110071761959 1102 42-43 0.5906679347636228 1102 44-45 0.5732283273659107 1102 46-47 0.7344068777461175 1102 48-49 0.6338825740134695 1102 50-51 0.5853394936878047 1102 52-53 0.26927595642214186 1102 54-55 0.2055224175802124 1102 56-57 0.2244335377318265 1102 58-59 0.3489040705381967 1102 60-61 0.4128223032975278 1102 62-63 0.2720642560708342 1102 64-65 0.18007965947171556 1102 66-67 0.19642642969232682 1102 68-69 -6.766810244038712 1102 70-71 -7.671985446481184 1102 72-73 -1.4100435406600624 1102 74-75 0.21129256319745338 1102 76-77 0.7147197231646523 1102 78-79 0.31163258898194 1102 80-81 0.14288018460628038 1102 82-83 0.13834427479401512 1102 84-85 -0.14106859140909833 1102 86-87 -0.12604071906654113 1102 88-89 -0.1775152844367902 1102 90-91 -0.12302631354502935 1102 92-93 -0.06301739747896207 1102 94-95 -0.0879805045257811 1102 96-97 -0.1258408249987717 1102 98-99 -0.2970950518655471 1102 100-101 -0.3618688571545867 1103 1 0.34778801895536304 1103 2 0.27599714087406113 1103 3 0.33819527453445986 1103 4 0.30495820616393843 1103 5 0.414573132665609 1103 6 0.3409602421940079 1103 7 0.3483596927402246 1103 8 0.5068384872388378 1103 9 0.5408333532833183 1103 10-11 0.5794713119401109 1103 12-13 0.4833804607297054 1103 14-15 0.6900024489466432 1103 16-17 0.7383503574015293 1103 18-19 0.6401983699514631 1103 20-21 0.5205383550295153 1103 22-23 0.38888140944327887 1103 24-25 0.5434364756417764 1103 26-27 0.7711514785529872 1103 28-29 0.5999859085599937 1103 30-31 0.6291069167733525 1103 32-33 0.6705598912530704 1103 34-35 0.6347290513801127 1103 36-37 0.8197669841891155 1103 38-39 0.951302334326769 1103 40-41 0.8584177033635925 1103 42-43 0.5943057454117096 1103 44-45 0.9939371070405514 1103 46-47 0.9002725279505128 1103 48-49 0.9990090368777516 1103 50-51 0.6983242467537636 1103 52-53 0.5548157122138022 1103 54-55 0.4058921781919338 1103 56-57 0.7252243859290388 1103 58-59 0.7173831773335131 1103 60-61 0.788879195802874 1103 62-63 0.44177791165621727 1103 64-65 0.47287849415818783 1103 66-67 0.38114313272192035 1103 68-69 -6.608117014828412 1103 70-71 -7.558456463586936 1103 72-73 -0.9580277352302708 1103 74-75 0.7645314743249969 1103 76-77 0.8749680898681085 1103 78-79 0.49885584523071813 1103 80-81 0.2599100859832113 1103 82-83 0.08855361964207376 1103 84-85 -0.10825195770582141 1103 86-87 -0.0064760361525024734 1103 88-89 0.19454532813591996 1103 90-91 -0.11375771229763743 1103 92-93 -0.20235844533840464 1103 94-95 0.10978557317069004 1103 96-97 0.1612126364000659 1103 98-99 0.26026595250407425 1103 100-101 0.4194222332598052 1104 1 0.21404711254090003 1104 2 0.19480420751843752 1104 3 0.16237111329084541 1104 4 0.2433134779151942 1104 5 0.4041305282904304 1104 6 0.3800797982421571 1104 7 0.42686476079339997 1104 8 0.5833052476092817 1104 9 0.644378970774369 1104 10-11 0.41077459342972134 1104 12-13 0.40894824868673396 1104 14-15 0.6330889061567717 1104 16-17 0.6271257776458512 1104 18-19 0.5593974415233873 1104 20-21 0.5551169047675586 1104 22-23 0.5490614191674794 1104 24-25 0.5013810842693047 1104 26-27 0.7210543839891344 1104 28-29 0.6196693767436159 1104 30-31 0.6395267438783989 1104 32-33 0.8147885143059099 1104 34-35 0.4514280485020663 1104 36-37 0.7412210792179508 1104 38-39 0.7865703350780464 1104 40-41 0.7428037446268689 1104 42-43 0.5852207105647054 1104 44-45 0.6923011830544255 1104 46-47 0.6867379054679148 1104 48-49 0.7507162264456753 1104 50-51 0.6307414325800167 1104 52-53 0.4601475541887865 1104 54-55 0.48051103116694094 1104 56-57 0.4198594763702772 1104 58-59 0.5353270370385026 1104 60-61 0.6190369059161895 1104 62-63 0.37727688424654104 1104 64-65 0.2727450886452374 1104 66-67 0.4081296276435502 1104 68-69 -6.3634497708427205 1104 70-71 -3.667105074143972 1104 72-73 0.6540651081661935 1104 74-75 1.1958490855923287 1104 76-77 1.3001298674563948 1104 78-79 0.8801253809783525 1104 80-81 0.5515519482764084 1104 82-83 0.38665479141099723 1104 84-85 0.5295053406770087 1104 86-87 0.5316590848058595 1104 88-89 0.539614115491748 1104 90-91 0.4763390697237391 1104 92-93 0.42633593205191644 1104 94-95 0.5575662857026735 1104 96-97 0.24963340938837675 1104 98-99 0.40397711136145276 1104 100-101 0.7517857266473236 1105 1 0.1939992680181355 1105 2 0.17920584540757645 1105 3 0.2558933316003973 1105 4 0.22308694399477247 1105 5 0.2706357245431903 1105 6 0.30424433800055084 1105 7 0.26770401891226925 1105 8 0.3955888340859275 1105 9 0.42521962581361805 1105 10-11 0.36384607209904374 1105 12-13 0.33300379955355197 1105 14-15 0.33404346841140864 1105 16-17 0.3482613283962621 1105 18-19 0.39237509370768464 1105 20-21 0.38646350690426345 1105 22-23 0.3720067821194064 1105 24-25 0.39602484047178166 1105 26-27 0.5291537574121463 1105 28-29 0.5043536727393985 1105 30-31 0.3324725142654259 1105 32-33 0.5655784291228727 1105 34-35 0.4320093274408734 1105 36-37 0.4625472513942128 1105 38-39 0.4080732322327023 1105 40-41 0.4354325159480865 1105 42-43 0.3967379552556807 1105 44-45 0.6576157229583259 1105 46-47 0.6671495167864592 1105 48-49 0.7890415371876998 1105 50-51 0.474379842651814 1105 52-53 0.46514998424818543 1105 54-55 0.5531343748704671 1105 56-57 0.634028250543551 1105 58-59 0.7165101558378488 1105 60-61 0.831793626412793 1105 62-63 0.5705809792604057 1105 64-65 0.47843040904929524 1105 66-67 0.2817797078209594 1105 68-69 -2.8941866026855187 1105 70-71 -3.216378893819016 1105 72-73 0.6707391717202498 1105 74-75 1.2498086272860363 1105 76-77 1.1836928438280694 1105 78-79 0.673338821680538 1105 80-81 0.5459138466911959 1105 82-83 0.4359876891767982 1105 84-85 0.5617859150441014 1105 86-87 0.5970206252598018 1105 88-89 0.4992897534914711 1105 90-91 0.44557291710436786 1105 92-93 0.42927681321764766 1105 94-95 0.5540189082751574 1105 96-97 0.5804775567285532 1105 98-99 0.4735674644621817 1105 100-101 0.6087232552041577 1106 1 0.1340888464345653 1106 2 0.21675697632036872 1106 3 0.2028257711114243 1106 4 0.1726617113675104 1106 5 0.24312626348508104 1106 6 0.2116169006377575 1106 7 0.25569627349860014 1106 8 0.4132084730648984 1106 9 0.3847121717357993 1106 10-11 0.5124670824164497 1106 12-13 0.2711611444539983 1106 14-15 0.3861122472139229 1106 16-17 0.3536586928368095 1106 18-19 0.22839538716233676 1106 20-21 0.393874914321259 1106 22-23 0.37396358790139317 1106 24-25 0.3556643785720368 1106 26-27 0.6359721933800628 1106 28-29 0.48902337439972143 1106 30-31 0.4008374138869826 1106 32-33 0.6654076003753744 1106 34-35 0.44938044811276967 1106 36-37 0.6073046775155575 1106 38-39 0.6481470667421618 1106 40-41 0.5722735525151492 1106 42-43 0.6127303805236153 1106 44-45 0.7153156705767287 1106 46-47 0.745035801636412 1106 48-49 0.7385673071677488 1106 50-51 0.6712382710861888 1106 52-53 0.510466834054256 1106 54-55 0.49919795671230105 1106 56-57 0.6941518769980206 1106 58-59 1.0180170095872505 1106 60-61 1.152994194959117 1106 62-63 0.8045121886867364 1106 64-65 0.7695287746257584 1106 66-67 0.8441271979184819 1106 68-69 -2.913357825766383 1106 70-71 -2.8708862125286636 1106 72-73 1.2722089463616726 1106 74-75 1.7620076538123115 1106 76-77 1.7856882001112133 1106 78-79 1.4367120199595966 1106 80-81 1.2817777310514593 1106 82-83 0.9857056231053605 1106 84-85 0.9091864047237515 1106 86-87 0.81406333097528 1106 88-89 0.8969971757871988 1106 90-91 1.155648723280688 1106 92-93 0.9989327132099959 1106 94-95 1.315272564687195 1106 96-97 1.1548041000728553 1106 98-99 1.2325526740179988 1106 100-101 1.3384746780413543 1107 1 0.046747600808796363 1107 2 0.07882718086336027 1107 3 0.15059745470255237 1107 4 -0.042417198402397105 1107 5 0.26368409471793086 1107 6 0.20252708223315352 1107 7 0.06784631960086074 1107 8 0.061010972696443844 1107 9 0.15293992054487404 1107 10-11 0.1414620531899402 1107 12-13 0.23613682657862256 1107 14-15 0.06405082463344058 1107 16-17 0.07474794851371769 1107 18-19 0.18032053707079854 1107 20-21 0.2497794747298343 1107 22-23 0.33436071287444946 1107 24-25 0.2344595931941882 1107 26-27 0.6290391932984463 1107 28-29 0.3998075878172429 1107 30-31 0.4816976990754327 1107 32-33 0.4493766515701978 1107 34-35 0.25760084004895845 1107 36-37 0.25814919536632885 1107 38-39 0.46807648826540316 1107 40-41 0.3549090182454009 1107 42-43 0.06493917807684824 1107 44-45 0.375264927642192 1107 46-47 0.46691646926044683 1107 48-49 0.4543129335433562 1107 50-51 0.5670591957866478 1107 52-53 0.08958138446283925 1107 54-55 0.18171661381503412 1107 56-57 0.3730083887440543 1107 58-59 0.5340999680732956 1107 60-61 0.4064852077593457 1107 62-63 0.11978114085610159 1107 64-65 0.010764321232883844 1107 66-67 0.10068519845684776 1107 68-69 -2.59518692828879 1107 70-71 -2.6206153541496846 1107 72-73 1.0788189565809034 1107 74-75 1.254437017479347 1107 76-77 0.7029188124977956 1107 78-79 0.729052254624353 1107 80-81 0.41960932321761035 1107 82-83 0.4998656241284962 1107 84-85 0.41580530652776915 1107 86-87 0.448654671302517 1107 88-89 0.3502204361729966 1107 90-91 0.4293714410362064 1107 92-93 0.38324284194309044 1107 94-95 0.8710515771366296 1107 96-97 0.8295914937544673 1107 98-99 1.0276911695862125 1107 100-101 1.2190771377216727 1108 1 -0.022963572419534728 1108 2 -0.024263913614003485 1108 3 -0.06719510184822042 1108 4 -0.04814778661830843 1108 5 -0.12126114388725284 1108 6 -0.15853521377309931 1108 7 0.06957895072891063 1108 8 0.20983053193265988 1108 9 0.15014510324455443 1108 10-11 0.19339430758741116 1108 12-13 0.04971926339204913 1108 14-15 0.11847997164440471 1108 16-17 0.0609811736605792 1108 18-19 0.20522585102209234 1108 20-21 0.06552701399090921 1108 22-23 0.21463022717025382 1108 24-25 0.23227664272382498 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1.0213016173543004 2311 66-67 1.2477271956413851 2311 68-69 3.5144293647933544 2311 70-71 4.899205056272045 2311 72-73 3.799786875479274 2311 74-75 2.60048585721308 2311 76-77 2.1368256266882533 2311 78-79 1.5590919823804192 2311 80-81 1.3790472988553368 2311 82-83 1.256905463424097 2311 84-85 1.1427568108898392 2311 86-87 1.150523815021561 2311 88-89 1.1640839249107842 2311 90-91 1.3730568273894512 2311 92-93 1.4261866544517012 2311 94-95 1.7559015017927742 2311 96-97 1.6990442246868582 2311 98-99 1.8945918870113836 2311 100-101 2.1584776382644257 2312 1 -0.26052381110007516 2312 2 -0.13309350538075293 2312 3 -0.15793630384228052 2312 4 -0.02348864279270657 2312 5 -1.3518405542611163 2312 6 -0.30165564059687 2312 7 0.00528338682266849 2312 8 0.14754003619764688 2312 9 -2.7704784550881456 2312 10-11 -0.22048188680800251 2312 12-13 -1.1877281687706258 2312 14-15 -4.114269294662009 2312 16-17 -0.9850714104208862 2312 18-19 -0.1891384255903148 2312 20-21 0.053772845564459715 2312 22-23 0.09406749411198945 2312 24-25 0.26919848675843383 2312 26-27 0.4710439621635132 2312 28-29 0.3402440201746586 2312 30-31 0.08923929745726866 2312 32-33 0.5067052750956194 2312 34-35 0.359276217645629 2312 36-37 0.3654294658480879 2312 38-39 0.5234171003927912 2312 40-41 0.2606394075796814 2312 42-43 -0.029403515815168646 2312 44-45 0.6535064271032951 2312 46-47 0.697123833687364 2312 48-49 0.8595398606520632 2312 50-51 1.0602059823393333 2312 52-53 1.0277293521585804 2312 54-55 0.8148469381864416 2312 56-57 0.7498465426174619 2312 58-59 0.7935808557022987 2312 60-61 0.8636816909059135 2312 62-63 0.8515915378678116 2312 64-65 0.9223627768883489 2312 66-67 1.3450814128933715 2312 68-69 3.9793491039564124 2312 70-71 4.648697442058847 2312 72-73 2.8491368317045556 2312 74-75 2.2889396824333588 2312 76-77 1.9478096305870025 2312 78-79 1.5325990057427497 2312 80-81 1.2301668965592114 2312 82-83 1.2864692472848063 2312 84-85 1.3321183684934859 2312 86-87 1.4273623531616337 2312 88-89 1.2675943122415596 2312 90-91 1.4612556594886144 2312 92-93 1.5681688728595127 2312 94-95 1.5319332266029058 2312 96-97 1.431258750992658 2312 98-99 1.8016886744215164 2312 100-101 1.6984625004064107 2313 1 -0.30633810166170505 2313 2 -0.19876247742715236 2313 3 -0.1817108988648357 2313 4 -0.07335459285071977 2313 5 0.09119115273922063 2313 6 -0.03648244388276822 2313 7 -2.5472049152253646 2313 8 -3.103421981589104 2313 9 -0.9461504851488414 2313 10-11 -0.23579175162701205 2313 12-13 -0.033377619794222824 2313 14-15 -0.6616408685339934 2313 16-17 -0.3168371623363413 2313 18-19 0.11675107955594655 2313 20-21 -2.5436109813100387 2313 22-23 -0.5865570529458282 2313 24-25 -0.08805214893152424 2313 26-27 -1.6852835092775607 2313 28-29 -1.7316440313675443 2313 30-31 -1.040657923938781 2313 32-33 -0.9279615060720445 2313 34-35 -1.354907418207702 2313 36-37 -1.8856529874334171 2313 38-39 -1.6592931799810415 2313 40-41 -0.9607741281132078 2313 42-43 -0.10188955577042691 2313 44-45 -0.170220098447885 2313 46-47 -0.21518331243125743 2313 48-49 -1.8739472810261546 2313 50-51 -0.344472570088854 2313 52-53 -0.47826634567609716 2313 54-55 -0.33996126567000573 2313 56-57 -0.2510059312323243 2313 58-59 -0.11515700125803363 2313 60-61 0.039126883330414586 2313 62-63 0.31789211553928 2313 64-65 0.23599795997124318 2313 66-67 0.8211848114783074 2313 68-69 1.5867286579890134 2313 70-71 4.192422229149837 2313 72-73 3.3559820710465367 2313 74-75 1.9194569895375864 2313 76-77 1.8035691701254528 2313 78-79 1.0539535804064748 2313 80-81 0.8688153799097904 2313 82-83 0.8198475781116912 2313 84-85 0.7134947472180819 2313 86-87 0.7848962740805447 2313 88-89 0.517616095357134 2313 90-91 1.0077794722040885 2313 92-93 1.0534691447295508 2313 94-95 1.3439403312498293 2313 96-97 1.2974704158510484 2313 98-99 1.4812333863607599 2313 100-101 1.4504439261113227 2314 1 -0.30002452676901825 2314 2 -0.3105541435890551 2314 3 -0.28847431849244387 2314 4 -0.12326131265883333 2314 5 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66-67 -1.4077602137800334 2314 68-69 -1.7866166281308118 2314 70-71 0.9820307753921789 2314 72-73 0.6672748295154527 2314 74-75 0.1443808712857617 2314 76-77 0.08508545781610266 2314 78-79 -1.9055587782094285 2314 80-81 -1.30941591054696 2314 82-83 -0.40191376189259387 2314 84-85 -0.2626467237015113 2314 86-87 -0.6093114701944309 2314 88-89 -0.7577403682333035 2314 90-91 -0.10883358863801718 2314 92-93 -0.6244660517847613 2314 94-95 -0.2196159861612621 2314 96-97 0.0376366241929631 2314 98-99 -0.30808441642633966 2314 100-101 -0.14505227605229365 2315 1 -0.45856406438725017 2315 2 -0.3424150832235071 2315 3 -0.46155622957819276 2315 4 -0.19822629683686444 2315 5 -0.1823525100614276 2315 6 -0.19530731692025682 2315 7 -1.0717943034566346 2315 8 -2.985850998297483 2315 9 -0.8580912414264219 2315 10-11 -1.3896923375945889 2315 12-13 -0.6558681583664807 2315 14-15 -0.3441161922529119 2315 16-17 -0.3897165034303782 2315 18-19 -0.18094471113034416 2315 20-21 -0.556440260073515 2315 22-23 -0.42974813722243255 2315 24-25 -5.094904962754544 2315 26-27 -1.128869934548412 2315 28-29 -1.090028460855713 2315 30-31 -1.2036181657246061 2315 32-33 -0.4249744565491298 2315 34-35 -2.1106688018173045 2315 36-37 -3.346927701353735 2315 38-39 -1.1924025849714468 2315 40-41 -0.2936648599145357 2315 42-43 -1.811304784044843 2315 44-45 -5.178718176911275 2315 46-47 -3.417121775941851 2315 48-49 -2.3660884242780647 2315 50-51 -1.1266498125913316 2315 52-53 -0.4217571672953824 2315 54-55 -1.6306160463687789 2315 56-57 -1.5406556368720175 2315 58-59 -0.9778404763873176 2315 60-61 -1.8471682203000732 2315 62-63 -0.7012296901803339 2315 64-65 -0.32783140396443144 2315 66-67 -0.038500214817535294 2315 68-69 2.8802419771155066 2315 70-71 3.241643699256546 2315 72-73 -2.6220418864327435 2315 74-75 -2.2256023557032236 2315 76-77 -0.6773231119821013 2315 78-79 -0.5497919749378255 2315 80-81 -0.78866147088657 2315 82-83 -1.3941463015339615 2315 84-85 -1.0877400992267532 2315 86-87 -0.7422916192494391 2315 88-89 -0.6772376966557765 2315 90-91 -0.5364321084630213 2315 92-93 -1.7871071958078595 2315 94-95 -0.7296274851842774 2315 96-97 -1.108573885741155 2315 98-99 -3.201892235761992 2315 100-101 -4.7274146768407945 2316 1 -0.5272590499219056 2316 2 -0.3978837472451886 2316 3 -0.8494624202968701 2316 4 -0.34728227239407516 2316 5 -0.12022288716757856 2316 6 -0.5219661680121064 2316 7 -0.46342455029076746 2316 8 -0.9728345221704231 2316 9 -4.740716323266124 2316 10-11 -1.4594538040127958 2316 12-13 -0.6080695763617001 2316 14-15 -0.2877827320796982 2316 16-17 -0.35943791269603054 2316 18-19 -0.40629440680790196 2316 20-21 -3.709399592970364 2316 22-23 -1.0197722741050441 2316 24-25 -0.34245560077778947 2316 26-27 -0.4425344598594947 2316 28-29 -0.006073518856979376 2316 30-31 0.02082313259345625 2316 32-33 -1.271085974915735 2316 34-35 -0.5914991926520088 2316 36-37 -0.37464664470294196 2316 38-39 -5.289273743635075 2316 40-41 -4.8235438117694756 2316 42-43 -3.6274489823328793 2316 44-45 -2.2798216838473664 2316 46-47 -2.5598108093287166 2316 48-49 -2.799015310415026 2316 50-51 -1.2381541894997596 2316 52-53 -1.031090343944161 2316 54-55 -0.27477959333404556 2316 56-57 -3.746963262746604 2316 58-59 -1.1269873849450924 2316 60-61 -0.7506953015466102 2316 62-63 -0.6274928195066494 2316 64-65 -0.20361062983081268 2316 66-67 -0.15662358152095024 2316 68-69 2.5918090278458656 2316 70-71 3.405208617957779 2316 72-73 2.1241195751357047 2316 74-75 0.8282283485005877 2316 76-77 -1.461730527265189 2316 78-79 -0.19488980019351132 2316 80-81 -0.10249433882946946 2316 82-83 0.02149258906477769 2316 84-85 0.12862005327954762 2316 86-87 0.20216254025917024 2316 88-89 -0.06933519921589237 2316 90-91 0.062403902497432995 2316 92-93 -0.21589173402676565 2316 94-95 -0.0973062128310822 2316 96-97 -0.013390350303399856 2316 98-99 0.08126893109027478 2316 100-101 0.32809339148112926 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 37.0 4 86.0 5 207.0 6 502.0 7 1258.0 8 2614.0 9 4548.0 10 7271.0 11 10941.0 12 14435.0 13 17937.0 14 22045.0 15 28527.0 16 34097.0 17 38151.0 18 32927.0 19 26284.0 20 24980.0 21 25920.0 22 27706.0 23 30835.0 24 36418.0 25 44544.0 26 55546.0 27 69123.0 28 84296.0 29 100574.0 30 118794.0 31 137826.0 32 160945.0 33 186277.0 34 217935.0 35 248512.0 36 262977.0 37 234362.0 38 115364.0 39 8199.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.6311536535693 22.07623603390424 16.172469617652308 21.12014069487416 2 14.911404415349717 22.644021781580705 40.27010738684341 22.174466416226178 3 17.906197723788424 27.87278205007008 31.737368423215877 22.48365180292562 4 11.812001342918082 19.138475261342393 39.12581957500593 29.9237038207336 5 11.634820614983846 40.178223901534196 34.797063880210786 13.38989160327118 6 28.206988307062 39.55257835263113 18.272820423451307 13.967612916855563 7 25.964125873867193 35.4338068077897 21.78908650899895 16.812980809344158 8 25.68739013081343 35.68962193696936 20.251441939953317 18.371545992263894 9 26.354178183622164 17.049807090567352 21.765864216584664 34.830150509225824 10-11 23.4940588597516 28.683071223801722 27.826553069883726 19.99631684656295 12-13 24.851880820729246 26.632681286983555 28.620160155724996 19.895277736562207 14-15 22.08265327637775 27.501667689406567 27.89934430932587 22.516334724889816 16-17 19.675814799226632 31.011610395759625 28.608581532532785 20.70399327248096 18-19 19.482015145465144 28.653696015072715 32.50989362744967 19.354395212012466 20-21 20.841000457458613 27.479740091171127 32.47504014579489 19.204219305575364 22-23 20.33627975950718 27.75948991452542 32.238952340398946 19.66527798556846 24-25 19.87948563548515 28.0481975251808 32.61093219833902 19.461384640995025 26-27 19.924288898273996 28.13214094513514 32.517322202732714 19.42624795385815 28-29 19.44031142081844 28.21048936735145 32.77847148638418 19.570727725445934 30-31 19.779046807978943 28.050364898701357 32.73701975007028 19.433568543249415 32-33 19.65010607298948 28.199077309874728 32.35383392769692 19.79698268943888 34-35 19.955884631714977 28.111946086605542 32.31404486507882 19.61812441660066 36-37 20.097481628604704 27.78670710300282 32.32880138652972 19.787009881862765 38-39 19.6852431443429 28.341817831723453 32.26163140179164 19.71130762214201 40-41 19.846303324280896 28.195549032464534 32.367539248924935 19.590608394329628 42-43 19.684838058520494 28.22857622395573 31.906110018927077 20.180475698596698 44-45 19.947152097842046 28.268670721204998 31.6565392337157 20.12763794723725 46-47 20.223515072335115 28.498439104935997 31.034662195487655 20.24338362724123 48-49 20.023491283524493 28.705167938408493 30.827486734993354 20.44385404307366 50-51 20.153319618177633 28.853654709198047 30.527238253972794 20.46578741865153 52-53 20.234696246476297 29.220439399506944 30.247883207896464 20.296981146120295 54-55 20.1586479151246 28.94376593469858 30.246566329467882 20.651019820708942 56-57 20.36674673384498 29.165708224512894 29.8089590958473 20.658585945794826 58-59 20.327817094004093 28.77648346782888 30.245125919610178 20.65057351855685 60-61 20.257426381559057 29.13671275340461 30.302867744853394 20.30299312018294 62-63 20.24763241169916 28.89799713188991 30.50207134434132 20.352299112069613 64-65 20.12054115687614 28.949628051241703 30.27101413890307 20.658816652979084 66-67 20.048447478528956 29.090097080185313 29.9186024734146 20.942852967871133 68-69 20.356590138614624 28.64869831128416 30.212140155406804 20.782571394694415 70-71 19.910533318411858 28.72506351989323 30.734716885722218 20.629686275972695 72-73 20.242735734336843 28.809794388530186 29.996294378528926 20.95117549860405 74-75 20.06579508736868 29.241351801191918 29.671548867759075 21.02130424368033 76-77 19.350855156002584 29.226588803489843 30.321673553195787 21.100882487311786 78-79 19.82913002737529 29.085467255402865 29.75779478379401 21.32760793342784 80-81 19.81655067842997 29.308939188021377 29.635890171268553 21.238619962280104 82-83 20.051578923788842 29.33342183968116 29.415214857722276 21.199784378807717 84-85 19.805014786241706 29.079451131419788 29.41545537308801 21.700078709250494 86-87 19.564378370380116 29.152205543864262 29.580593186899907 21.702822898855715 88-89 19.877439224133855 28.91232500492191 29.411298082159576 21.798937688784655 90-91 19.657537188543824 28.974685217043895 29.62331269204139 21.74446490237089 92-93 19.86543217259899 28.95853281239114 29.55483775634793 21.62119725866194 94-95 19.482364728593453 28.574986329366535 29.71229666025242 22.230352281787592 96-97 19.697947117618277 28.429830728684845 29.561403508771928 22.310818644924943 98-99 19.496985129433796 28.474983367188155 29.398795531615907 22.629235971762142 100-101 19.225282304002683 28.2995825018688 29.78570566803092 22.68942952609759 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3281.0 1 2191.0 2 1187.5 3 1306.5 4 1208.0 5 1233.5 6 2006.0 7 3841.0 8 5735.0 9 6571.0 10 6155.0 11 5319.5 12 5072.5 13 5134.5 14 5166.5 15 5270.0 16 5559.0 17 5833.5 18 6176.0 19 6836.5 20 7716.0 21 8749.5 22 9981.0 23 11472.5 24 13202.0 25 16058.0 26 21050.0 27 26417.5 28 30085.5 29 34212.0 30 40169.0 31 47565.0 32 57126.0 33 67002.5 34 77149.0 35 85612.5 36 94535.5 37 101909.5 38 100523.5 39 95817.0 40 91985.5 41 89623.0 42 87111.5 43 85452.5 44 85619.5 45 84844.5 46 81794.5 47 79190.0 48 80792.0 49 77748.0 50 68562.0 51 60351.5 52 54096.0 53 50065.0 54 46819.5 55 43342.0 56 39263.0 57 34488.5 58 30535.0 59 27167.0 60 23601.0 61 19893.5 62 15979.0 63 12197.0 64 8942.5 65 6587.5 66 4746.5 67 3480.5 68 2345.0 69 1455.0 70 1115.5 71 876.0 72 709.5 73 671.5 74 565.5 75 417.5 76 286.0 77 172.5 78 101.0 79 71.0 80 55.5 81 37.5 82 22.5 83 13.0 84 9.0 85 5.0 86 3.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020920613874107225 2 0.020262991434056705 3 6.987238425536795E-4 4 0.10189037680532773 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.012700333373475703 12-13 0.0 14-15 0.0 16-17 6.165210375473642E-4 18-19 6.165210375473642E-5 20-21 0.0 22-23 1.2330420750947285E-4 24-25 0.004192343055322077 26-27 0.003062054486485242 28-29 0.0011919406725915708 30-31 1.2330420750947285E-4 32-33 5.137675312894701E-4 34-35 0.002794895370214718 36-37 0.09001207148191519 38-39 0.10258910064788142 40-41 0.08158628396876787 42-43 0.08623074245162468 44-45 0.08501825107778152 46-47 0.08343584708140996 48-49 0.0777844042372258 50-51 0.07712678179717526 52-53 0.059124367500792224 54-55 0.04960939282131124 56-57 0.027722895988379813 58-59 0.021475482807899855 60-61 0.012803086879733597 62-63 0.0018906645151452503 64-65 0.005959703362957854 66-67 0.146814209741279 68-69 0.6865372767114932 70-71 0.8375232788068429 72-73 0.3416554083074977 74-75 0.049917653340084925 76-77 0.1838671240978756 78-79 0.013912824747318853 80-81 0.0030209530839820846 82-83 0.001500201191365253 84-85 8.220280500631523E-5 86-87 0.001931765917648408 88-89 4.110140250315761E-4 90-91 0.00413069095156734 92-93 0.004418400769089444 94-95 0.06989293495661952 96-97 0.033579845845079774 98-99 0.012967492489746229 100-101 0.01239207285470202 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2433007.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.97092979221219 #Duplication Level Percentage of deduplicated Percentage of total 1 69.24740949144666 27.678833430751826 2 14.099917752663233 11.271736451353368 3 5.920725058441425 7.0997065688986085 4 3.1964412055920794 5.1105890805462035 5 1.8837104984284665 3.764683004076864 6 1.1758561848010833 2.820003900505355 7 0.8496210221969327 2.3772099559754807 8 0.6064554385250129 1.9392470204310837 9 0.45454998863815477 1.6351907114615878 >10 2.244195735196774 15.933356999574317 >50 0.18367273983978072 5.121377015478055 >100 0.12633658104088716 9.620919366061779 >500 0.008698010988048776 2.2841341558751527 >1k 0.0019911109490713935 1.2519384128148947 >5k 1.0479531310902071E-4 0.21308253165114324 >10k+ 3.143859393270622E-4 1.877991394544399 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18576 0.7634996528986558 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15103 0.6207544820051895 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11155 0.45848614492272316 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5087 0.2090828345335628 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3712 0.15256840609172106 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3122 0.12831857861485807 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.0550701251578806E-5 0.0 16-17 0.0 0.0 0.0 8.220280500631523E-5 0.0 18-19 0.0 0.0 0.0 1.2330420750947285E-4 0.0 20-21 0.0 0.0 0.0 2.877098175221033E-4 0.0 22-23 0.0 0.0 0.0 9.042308550694675E-4 0.0 24-25 0.0 0.0 0.0 0.0017057082038810412 0.0 26-27 0.0 0.0 0.0 0.0032470107977494514 0.0 28-29 0.0 0.0 0.0 0.006555673699253639 0.0 30-31 0.0 0.0 0.0 0.015741837158709367 0.0 32-33 0.0 2.0550701251578806E-5 0.0 0.0299629224248019 0.0 34-35 0.0 4.110140250315761E-5 0.0 0.05293860642406701 0.0 36-37 0.0 4.110140250315761E-5 0.0 0.08951885465187728 0.0 38-39 0.0 4.110140250315761E-5 0.0 0.14237525827093797 0.0 40-41 0.0 4.110140250315761E-5 0.0 0.19930070073781128 0.0 42-43 0.0 4.110140250315761E-5 0.0 0.25764414159104354 0.0 44-45 0.0 4.110140250315761E-5 0.0 0.3277014821576757 0.0 46-47 0.0 4.110140250315761E-5 0.0 0.40926721542519195 0.0 48-49 0.0 4.110140250315761E-5 0.0 0.4984983602595471 0.0 50-51 0.0 4.110140250315761E-5 0.0 0.5984364204459749 0.0 52-53 0.0 4.110140250315761E-5 0.0 0.6965249175197605 0.0 54-55 0.0 4.110140250315761E-5 0.0 0.8041283892730271 0.0 56-57 0.0 4.110140250315761E-5 0.0 0.9255830336698578 0.0 58-59 0.0 4.110140250315761E-5 0.0 1.0512505718232623 0.0 60-61 0.0 4.110140250315761E-5 0.0 1.182466799314593 0.0 62-63 0.0 4.110140250315761E-5 0.0 1.3109086821369607 0.0 64-65 0.0 8.220280500631523E-5 0.0 1.446337803384865 0.0 66-67 0.0 8.220280500631523E-5 0.0 1.5896172925108725 0.0 68-69 0.0 8.220280500631523E-5 0.0 1.7515773690745648 0.0 70-71 0.0 8.220280500631523E-5 0.0 1.9538373707926036 0.0 72-73 0.0 8.220280500631523E-5 0.0 2.176072654127177 0.0 74-75 0.0 8.220280500631523E-5 0.0 2.409734127357628 0.0 76-77 0.0 8.220280500631523E-5 0.0 2.675105332619265 0.0 78-79 0.0 8.220280500631523E-5 0.0 2.9813313319690407 0.0 80-81 0.0 8.220280500631523E-5 0.0 3.3219181038114565 0.0 82-83 0.0 8.220280500631523E-5 0.0 3.670889561764516 0.0 84-85 0.0 8.220280500631523E-5 0.0 4.02421776838291 0.0 86-87 0.0 8.220280500631523E-5 0.0 4.3890338170009375 0.0 88-89 0.0 8.220280500631523E-5 0.0 4.78033971953225 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12490 0.0 36.336945 1 GTATCAA 19070 0.0 31.26741 1 CCGCGTA 35 0.007985729 27.421831 66-67 GCGTATC 90 8.9794263E-4 26.374271 1 CATTCCG 555 0.0 24.7765 9 ATCAACG 24130 0.0 24.565916 3 GTACATG 16040 0.0 24.447174 1 TCAACGC 24270 0.0 24.349998 4 TATCAAC 24635 0.0 24.142378 2 CAACGCA 24590 0.0 24.026718 5 AACGCAG 25160 0.0 23.576622 6 TACATGG 16755 0.0 23.088444 2 ACATGGG 16790 0.0 22.104666 3 GAGTACT 15015 0.0 21.975487 12-13 AGTACTT 15620 0.0 21.518888 12-13 CGCAGAG 28365 0.0 20.879234 8 ACGCAGA 28440 0.0 20.874191 7 GTATAGG 865 0.0 20.855494 1 GTACTTT 16330 0.0 20.162329 14-15 GCAGAGT 28940 0.0 19.956469 9 >>END_MODULE