FastQCFastQC Report
Fri 27 May 2016
SRR939397_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939397_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2377010
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87610.36857228198451No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70450.2963807472412821No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47560.20008329792470372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69900.042.6381531
GTATCAA114800.037.7940441
TATCAAC147900.029.3526152
ATCAACG151950.028.4425243
TCAACGC154250.028.060674
CAACGCA154850.027.8005775
AACGCAG160250.027.2495826
CGCAGAG177450.024.2081878
ACGCAGA177150.024.1417227
GTACATG100550.023.3565481
TACATGG102350.022.7295132
GCAGAGT178950.022.1452629
GAGTACT96050.021.99558312-13
AGAGTAC149700.021.51050410-11
GTACTTT101150.020.93920314-15
ACATGGG107200.020.7605693
AGTACTT101950.020.46451212-13
CAGAGTA176750.019.00641410-11
GCACCGT5400.018.8268176
ACTTTTT121850.018.59349416-17
Produced by FastQC (version 0.11.5)