FastQCFastQC Report
Fri 27 May 2016
SRR939397_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939397_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2377010
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122790.5165733421399153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113610.47795339523182484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78190.3289426632618289No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30780.12949041022124433No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24690.10386998792600789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76250.042.9469871
GTATCAA121900.034.8336371
TATCAAC161400.026.303142
TCAACGC162800.026.0736544
ATCAACG162650.026.039453
CAACGCA165650.025.5964625
AACGCAG170550.024.9438696
GTACATG119400.022.6634651
TACATGG120450.021.94982
ACGCAGA194150.021.69227
CGCAGAG195850.021.479728
GAGTACT108050.020.84802612-13
AGTACTT111950.020.79881712-13
ACATGGG122400.020.7071693
GCAGAGT203950.020.1156169
AGAGTAC177900.019.9854510-11
GTACTTT112750.019.95796414-15
GTGGTAT43700.019.520261
TGGTATC43700.018.9740372
TACTTTT126100.018.20194814-15
Produced by FastQC (version 0.11.5)