##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939397_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2377010 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.903450553426364 31.0 31.0 31.0 28.0 34.0 2 31.171239919057975 31.0 31.0 34.0 28.0 34.0 3 31.152658592096795 31.0 31.0 34.0 28.0 34.0 4 35.09813210714301 35.0 35.0 37.0 32.0 37.0 5 34.706803084547396 35.0 35.0 37.0 32.0 37.0 6 34.67304344533679 35.0 35.0 37.0 31.0 37.0 7 34.54113108485029 35.0 35.0 37.0 30.0 37.0 8 34.55884745962364 35.0 35.0 37.0 30.0 37.0 9 35.90188850698988 37.0 35.0 39.0 30.0 39.0 10-11 35.752313620893474 37.0 35.0 39.0 30.0 39.0 12-13 35.709230503868305 37.0 35.0 39.0 30.0 39.0 14-15 36.542789681154055 38.0 35.0 40.0 30.0 41.0 16-17 36.545604772382106 38.0 35.0 40.0 30.0 41.0 18-19 36.50916634763842 38.0 35.0 40.0 30.0 41.0 20-21 36.40827110529615 38.0 35.0 40.0 30.0 41.0 22-23 36.254902167008126 38.0 34.0 40.0 29.5 41.0 24-25 36.02898157769635 38.0 34.0 40.0 29.0 41.0 26-27 35.634209363864684 38.0 34.0 40.0 27.0 41.0 28-29 35.33954022069743 38.0 34.0 40.0 27.0 41.0 30-31 35.09733194222994 37.5 33.0 40.0 27.0 41.0 32-33 34.892277693404736 37.0 33.0 40.0 26.0 41.0 34-35 34.848977076242846 37.0 33.0 40.0 26.0 41.0 36-37 34.84763884039192 38.0 33.0 40.0 25.5 41.0 38-39 34.811923172388845 38.0 33.0 40.0 25.0 41.0 40-41 34.592903689929784 37.5 33.0 40.0 25.0 41.0 42-43 34.27469131387752 37.0 33.0 40.0 24.0 41.0 44-45 34.06633228299418 37.0 33.0 40.0 24.0 41.0 46-47 33.704884918447966 37.0 32.5 40.0 23.0 41.0 48-49 33.37049381365665 37.0 32.0 40.0 22.0 41.0 50-51 33.081753757872285 37.0 31.0 40.0 20.5 41.0 52-53 32.71326919112667 36.0 31.0 39.0 19.0 40.5 54-55 32.46257609349561 36.0 31.0 39.0 18.5 40.0 56-57 32.040740047370434 35.5 30.0 39.0 16.0 40.0 58-59 31.58993041678411 35.0 29.5 39.0 13.5 40.0 60-61 31.27677060677069 35.0 29.0 38.0 12.5 40.0 62-63 30.874864009827473 35.0 29.0 38.0 8.0 40.0 64-65 30.412609328526173 34.0 28.0 38.0 8.0 40.0 66-67 28.60233360398147 33.0 25.0 36.5 4.5 39.5 68-69 24.802409750064157 29.0 13.5 35.0 2.0 38.0 70-71 24.14943858040143 28.5 10.5 34.0 2.0 38.0 72-73 26.06074900820779 30.5 21.0 35.0 2.0 37.5 74-75 26.704700653341803 31.0 22.0 35.0 2.0 37.0 76-77 26.85619644006546 31.0 24.5 34.0 2.0 36.0 78-79 27.71421092044207 32.5 26.0 35.0 2.0 37.0 80-81 27.572134320007066 32.5 26.0 35.0 2.0 36.0 82-83 27.155373767884864 32.0 25.5 35.0 2.0 36.0 84-85 26.81124816471113 32.0 25.0 35.0 2.0 35.5 86-87 26.353846639265292 32.0 24.5 34.0 2.0 35.0 88-89 25.849587717342374 31.5 23.5 34.0 2.0 35.0 90-91 25.36966630346528 31.0 21.0 34.0 2.0 35.0 92-93 24.75237378050576 31.0 19.0 34.0 2.0 35.0 94-95 23.288700299956666 30.0 7.0 34.0 2.0 35.0 96-97 22.448932061707776 29.5 2.0 34.0 2.0 35.0 98-99 22.08081097681541 30.0 2.0 34.0 2.0 35.0 100-101 20.80484137635096 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.36996268340417515 1101 2 0.13990356947997284 1101 3 0.3320634042715547 1101 4 0.1606514749200727 1101 5 0.29232917436329586 1101 6 0.4948753279012621 1101 7 0.46332609412642967 1101 8 0.4662359590617271 1101 9 0.5130145529493504 1101 10-11 0.734411851468721 1101 12-13 0.5696761616561261 1101 14-15 0.6382353714016489 1101 16-17 0.6028774488625572 1101 18-19 0.7792273154595719 1101 20-21 0.75231271983575 1101 22-23 0.8069312822593062 1101 24-25 0.6391674318874934 1101 26-27 0.8795795825705426 1101 28-29 0.7538269070901364 1101 30-31 0.7062323150879237 1101 32-33 0.6988791241991308 1101 34-35 0.673046238316303 1101 36-37 0.7783793193559703 1101 38-39 0.6747729836281664 1101 40-41 0.9005900256917059 1101 42-43 0.8703365929350184 1101 44-45 0.5455050646255657 1101 46-47 0.5767676738634222 1101 48-49 0.5339825486982122 1101 50-51 0.5877996233068146 1101 52-53 0.535943367030697 1101 54-55 0.5142483185922089 1101 56-57 0.4099468262379098 1101 58-59 0.5460136020934065 1101 60-61 0.4374993846913213 1101 62-63 0.35933944699935694 1101 64-65 0.13042802341252724 1101 66-67 -3.245379621160154 1101 68-69 -7.982480770628751 1101 70-71 -11.0295241049317 1101 72-73 -10.949682310329688 1101 74-75 -9.711568341091585 1101 76-77 -4.843082628714544 1101 78-79 -1.6960125171883682 1101 80-81 -0.9699020859359813 1101 82-83 -0.8022393026410874 1101 84-85 -0.8778956267655182 1101 86-87 -0.7543779001458084 1101 88-89 -0.7297336849548515 1101 90-91 -0.8986290687388099 1101 92-93 -1.5347983116174113 1101 94-95 -5.0264418890776845 1101 96-97 -1.9789576041728978 1101 98-99 -1.2594576306805187 1101 100-101 -0.9893860071067699 1102 1 0.46317636016310004 1102 2 0.29738878644843325 1102 3 0.43512861170156825 1102 4 0.28810193578907217 1102 5 0.49489221070601275 1102 6 0.5426894965447175 1102 7 0.4856353330146064 1102 8 0.4908035027873936 1102 9 0.6145028621196644 1102 10-11 0.6223034498440754 1102 12-13 0.6531985910295504 1102 14-15 0.6619604148078082 1102 16-17 0.6939661096461478 1102 18-19 0.6987180372954427 1102 20-21 0.8786060363780948 1102 22-23 0.8878996526502263 1102 24-25 0.8250247230313903 1102 26-27 0.9457316303749863 1102 28-29 0.7664164702854492 1102 30-31 0.6727887181589338 1102 32-33 0.4627675056870615 1102 34-35 0.6964074492624448 1102 36-37 0.9172543439034442 1102 38-39 0.8454979989800719 1102 40-41 0.8667089071375571 1102 42-43 1.008949964837754 1102 44-45 0.6837696910208706 1102 46-47 0.7574788179469394 1102 48-49 0.7858186994056453 1102 50-51 0.7252180711586433 1102 52-53 0.820448630319845 1102 54-55 0.8158191696023138 1102 56-57 0.8308007976288145 1102 58-59 0.6841507998478171 1102 60-61 0.7393687952715133 1102 62-63 0.6320498305009643 1102 64-65 0.5183770394876106 1102 66-67 -2.7544896468142106 1102 68-69 -7.5805868593362895 1102 70-71 -10.90338072277358 1102 72-73 -9.049768093994011 1102 74-75 -7.612451364217641 1102 76-77 -3.7372429805771894 1102 78-79 -1.1386206273605275 1102 80-81 -0.47802709567704227 1102 82-83 -0.21349579018655973 1102 84-85 -0.3096999826555127 1102 86-87 -0.17687516485636934 1102 88-89 -0.26343880984302714 1102 90-91 -0.4430607052055926 1102 92-93 -0.6997707716946557 1102 94-95 -4.442376391753751 1102 96-97 -1.2251400752536412 1102 98-99 -0.47512823637179125 1102 100-101 -0.2913106533894272 1103 1 0.4718121450701247 1103 2 0.28153000212299517 1103 3 0.4253346822150945 1103 4 0.27085551569444277 1103 5 0.4840514241485394 1103 6 0.49091188293871824 1103 7 0.578539654050779 1103 8 0.49429176887142035 1103 9 0.7112613776574719 1103 10-11 0.7543319319013193 1103 12-13 0.6798720690785558 1103 14-15 0.8177217439704805 1103 16-17 0.7565392403146589 1103 18-19 0.6943369561718882 1103 20-21 0.7825001560593208 1103 22-23 0.7769809019625384 1103 24-25 0.9859099931550901 1103 26-27 0.9900111695063813 1103 28-29 1.0601849985553073 1103 30-31 0.9960959062631147 1103 32-33 1.0287606838256664 1103 34-35 1.0670297476324322 1103 36-37 0.9518562710006861 1103 38-39 1.0283166537844508 1103 40-41 1.2178917298791916 1103 42-43 1.2221758879110496 1103 44-45 0.989005233861846 1103 46-47 0.949977914128894 1103 48-49 0.91458369099621 1103 50-51 0.8635918274264185 1103 52-53 1.0942904071475184 1103 54-55 1.0006604905913292 1103 56-57 0.9855595961009662 1103 58-59 0.9505734094455782 1103 60-61 0.8367824610003076 1103 62-63 0.8997292973269104 1103 64-65 0.6457018952696885 1103 66-67 -2.592440259052694 1103 68-69 -7.257121542371234 1103 70-71 -11.103020138671345 1103 72-73 -8.99215417362431 1103 74-75 -7.544297825180557 1103 76-77 -3.7174564388208005 1103 78-79 -0.7974291594028422 1103 80-81 -0.08017551045879046 1103 82-83 0.31516262946794527 1103 84-85 0.19172286272086936 1103 86-87 0.3401332760182143 1103 88-89 0.49304714561053586 1103 90-91 0.317370298111026 1103 92-93 0.5010267887825641 1103 94-95 1.2562183676734655 1103 96-97 1.4791451225646206 1103 98-99 1.3564909340018403 1103 100-101 1.0987517493518943 1104 1 0.406529659284498 1104 2 0.17905129578257117 1104 3 0.2684213779429676 1104 4 0.18138930014616506 1104 5 0.41407811496556235 1104 6 0.46440729575349593 1104 7 0.5179881713870529 1104 8 0.3971869412456286 1104 9 0.5884708638991043 1104 10-11 0.627560436545906 1104 12-13 0.5626576082043613 1104 14-15 0.8060798282267214 1104 16-17 0.8211019988121961 1104 18-19 0.7183478493416473 1104 20-21 0.8178691558074007 1104 22-23 0.6372909873922836 1104 24-25 0.6433615781814481 1104 26-27 0.8949551889457084 1104 28-29 0.9085470370066631 1104 30-31 0.9404508144077965 1104 32-33 0.7785962977304024 1104 34-35 0.8067151977888471 1104 36-37 0.966488706242778 1104 38-39 0.8528725243292072 1104 40-41 0.9276850594071462 1104 42-43 1.1656551331699987 1104 44-45 1.0902157277854627 1104 46-47 0.9359491314151 1104 48-49 1.0268936743822152 1104 50-51 0.9778530785465094 1104 52-53 0.9342577531491543 1104 54-55 0.8976192681072703 1104 56-57 0.865660428076378 1104 58-59 0.9812490347026817 1104 60-61 0.777026919546632 1104 62-63 0.8383589136105414 1104 64-65 0.6868430632241278 1104 66-67 -2.7533492137101945 1104 68-69 -6.980148988949736 1104 70-71 -8.66835013857568 1104 72-73 -8.237835331565705 1104 74-75 -7.04641587066018 1104 76-77 -3.508949817138415 1104 78-79 -0.491649019028916 1104 80-81 0.11831360434361216 1104 82-83 0.3612596156697201 1104 84-85 0.5109539631032156 1104 86-87 0.6353384955557644 1104 88-89 0.5216713727088909 1104 90-91 0.39606069420019807 1104 92-93 0.6316405500193305 1104 94-95 1.561816423740126 1104 96-97 1.8607670441121158 1104 98-99 1.5157270090167465 1104 100-101 1.344273782828619 1105 1 0.5678573018360318 1105 2 0.38770292476995394 1105 3 0.40980113290598297 1105 4 0.3640410116023247 1105 5 0.4063621793147689 1105 6 0.5427687328308011 1105 7 0.6435319580521153 1105 8 0.5041172636396354 1105 9 0.6396128928011393 1105 10-11 0.8746962501821827 1105 12-13 0.8607100360916391 1105 14-15 0.9216901855194166 1105 16-17 0.9179455391835987 1105 18-19 1.028820663771853 1105 20-21 1.1855648277021018 1105 22-23 1.1362272056491705 1105 24-25 1.2280559626469483 1105 26-27 1.1710340736013478 1105 28-29 1.3131021932359133 1105 30-31 1.1994383072981378 1105 32-33 1.1095485960893399 1105 34-35 1.2976376764860476 1105 36-37 1.0807309327426111 1105 38-39 0.9948246518599646 1105 40-41 1.1699364307777813 1105 42-43 1.0813137936021988 1105 44-45 1.0157244979469482 1105 46-47 1.0876489372207772 1105 48-49 0.9678392247009313 1105 50-51 0.9321015622010052 1105 52-53 0.995472230215313 1105 54-55 1.1976245696713548 1105 56-57 1.3297061367220486 1105 58-59 1.3443524394195059 1105 60-61 1.4201561252087025 1105 62-63 1.4020990950811196 1105 64-65 1.2625766916996781 1105 66-67 2.1296102502689536 1105 68-69 -4.554465856235883 1105 70-71 -4.994367466181579 1105 72-73 -1.3219875165627997 1105 74-75 1.338182300234756 1105 76-77 1.4830387341012035 1105 78-79 1.4323318534514478 1105 80-81 1.2870506081483022 1105 82-83 1.3787471193595415 1105 84-85 1.2859411182642901 1105 86-87 1.4208666655147475 1105 88-89 1.3552142378181173 1105 90-91 1.4251598013775357 1105 92-93 1.6968424577969898 1105 94-95 2.4700052458176707 1105 96-97 2.3760320797733137 1105 98-99 2.036051242204408 1105 100-101 2.0539720452900774 1106 1 0.1847168753161128 1106 2 0.17672037294173393 1106 3 0.14649592601741546 1106 4 0.1452038292977491 1106 5 0.24527008846017395 1106 6 0.31157003575797404 1106 7 0.2734786447596349 1106 8 0.301780250039414 1106 9 0.41310736764857836 1106 10-11 0.47341796674392356 1106 12-13 0.4434040919276896 1106 14-15 0.3792989557019908 1106 16-17 0.36789798288488385 1106 18-19 0.43702030653953017 1106 20-21 0.4737521282355104 1106 22-23 0.4516583250518025 1106 24-25 0.47343757054543545 1106 26-27 0.5753616628398461 1106 28-29 0.8153616096206591 1106 30-31 0.6539940377812101 1106 32-33 0.7079411185148032 1106 34-35 0.8341075530840669 1106 36-37 0.8450614004779808 1106 38-39 0.7156593344887128 1106 40-41 0.6550199105817995 1106 42-43 0.7235621169117152 1106 44-45 0.5164820721615939 1106 46-47 0.2861154587405963 1106 48-49 0.12646239347792942 1106 50-51 0.31509134285817453 1106 52-53 0.4573590306098865 1106 54-55 0.4193932702268981 1106 56-57 0.3254169048122826 1106 58-59 0.4900037027334001 1106 60-61 0.44479619429262485 1106 62-63 0.4572817811939309 1106 64-65 0.5039906582480143 1106 66-67 1.2134307895662033 1106 68-69 -5.402460922057735 1106 70-71 -6.430676581444331 1106 72-73 -2.800395802832032 1106 74-75 0.15400337449793966 1106 76-77 0.5291583641600432 1106 78-79 0.38106494143365666 1106 80-81 0.3356902128709365 1106 82-83 0.5186935277218474 1106 84-85 0.6013146677292909 1106 86-87 0.6003325278316254 1106 88-89 0.3734742271866871 1106 90-91 0.2862077643318237 1106 92-93 0.42944034875491255 1106 94-95 1.3126029263194532 1106 96-97 1.6334743180490392 1106 98-99 1.4064775380880903 1106 100-101 1.293180703048339 1107 1 -0.01005169239132897 1107 2 0.04568565381365275 1107 3 0.0048498234937390805 1107 4 -0.011785408800754738 1107 5 0.16789264504012635 1107 6 0.191477297185358 1107 7 0.14158123127184297 1107 8 -0.011082050174223923 1107 9 0.28242552350192796 1107 10-11 0.20866305134252627 1107 12-13 0.3091083852637695 1107 14-15 0.3120787708531907 1107 16-17 -0.030799621392453957 1107 18-19 0.3204509625356877 1107 20-21 0.3242441681984971 1107 22-23 0.2867580325839896 1107 24-25 0.49835952676666295 1107 26-27 0.4938481133545167 1107 28-29 0.3924699340999922 1107 30-31 0.35303459198487275 1107 32-33 0.33653141795156927 1107 34-35 0.3083700286641502 1107 36-37 0.30648978013776684 1107 38-39 0.2131816408715963 1107 40-41 0.21307712745353768 1107 42-43 0.5271102884166652 1107 44-45 0.3053248107342128 1107 46-47 0.07807066603858459 1107 48-49 0.1640487200365186 1107 50-51 0.0013032277903803902 1107 52-53 0.26408814658022095 1107 54-55 0.25388455045904124 1107 56-57 0.37095362894430295 1107 58-59 0.274798161268091 1107 60-61 0.15936974537738635 1107 62-63 0.33066335457935736 1107 64-65 0.12919087302906007 1107 66-67 1.1021506431038084 1107 68-69 -5.285383400950927 1107 70-71 -6.194647417490991 1107 72-73 -2.5725728743838268 1107 74-75 -0.07857751113115086 1107 76-77 0.12862005487533068 1107 78-79 0.33703175604791014 1107 80-81 0.18511056109042556 1107 82-83 0.21340754332774736 1107 84-85 0.1944853015618726 1107 86-87 0.2674189743477129 1107 88-89 0.3047447913296786 1107 90-91 0.29507505135013545 1107 92-93 0.2959996963003526 1107 94-95 0.919878277337947 1107 96-97 1.0406242849179215 1107 98-99 0.7873421746168638 1107 100-101 0.5824266559107549 1108 1 0.08019982717156893 1108 2 0.03498017960604827 1108 3 0.04158047092678885 1108 4 0.022707250977859417 1108 5 0.13162755575694263 1108 6 0.21893518426033154 1108 7 0.09508745131778085 1108 8 0.09861006700463548 1108 9 0.14115639307947703 1108 10-11 0.1659929926396586 1108 12-13 0.2784743045166067 1108 14-15 0.2261632046683104 1108 16-17 0.07885941043565481 1108 18-19 0.22174584045074397 1108 20-21 0.21777344164419077 1108 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1.1299024017066266 2312 92-93 1.204938926942038 2312 94-95 1.986813694350051 2312 96-97 2.4158177298966415 2312 98-99 2.235618096244014 2312 100-101 2.005278380334566 2313 1 -0.30976600308143176 2313 2 -0.2559800062518285 2313 3 -0.27109267308911456 2313 4 -0.13475101482764273 2313 5 -0.13684606248395426 2313 6 -0.16325942362585977 2313 7 -3.5363610904802805 2313 8 -1.2667910682612558 2313 9 -0.8276824742093751 2313 10-11 -0.08775570004817723 2313 12-13 -1.6856963605959265 2313 14-15 -0.5998796512199789 2313 16-17 -0.269592971467155 2313 18-19 -0.004536123830696681 2313 20-21 0.054950206216233255 2313 22-23 -1.4226689199574167 2313 24-25 -2.6994119774133836 2313 26-27 -2.3479741791531907 2313 28-29 -0.6721703516687896 2313 30-31 -0.4791173756555551 2313 32-33 -2.7803743164964416 2313 34-35 -2.877551925307685 2313 36-37 -1.3693021236099767 2313 38-39 -0.873134675603815 2313 40-41 -0.7588722749432364 2313 42-43 -0.9162199191550897 2313 44-45 -0.46931023011260464 2313 46-47 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2314 7 -0.9212100539071386 2314 8 -0.34342944910545725 2314 9 -0.04752102027355676 2314 10-11 0.024515527985769836 2314 12-13 -0.05907832853596062 2314 14-15 -2.555017871096183 2314 16-17 -2.7559504783462643 2314 18-19 -0.47996542243543416 2314 20-21 -0.27784656713475897 2314 22-23 -0.07047671809747413 2314 24-25 -2.32549182968728 2314 26-27 -1.3523115073274496 2314 28-29 -0.41359774295465 2314 30-31 -0.10377676130449487 2314 32-33 -0.07029103833767181 2314 34-35 -0.2755045946429817 2314 36-37 0.1407040113966218 2314 38-39 -1.35757818191194 2314 40-41 -3.892310794280654 2314 42-43 -5.6048859500488 2314 44-45 -2.4838222541180954 2314 46-47 -3.306221915539247 2314 48-49 -1.3047269022708932 2314 50-51 -1.4220119032160312 2314 52-53 -3.089575438816677 2314 54-55 -1.8977278118997205 2314 56-57 -4.402675532657838 2314 58-59 -5.5587141536219775 2314 60-61 -3.332285024147712 2314 62-63 -2.1389463412613736 2314 64-65 -1.2045194528507253 2314 66-67 -0.8646513872617732 2314 68-69 1.3490964032766648 2314 70-71 1.0937080529165932 2314 72-73 -1.0735966428948203 2314 74-75 -0.5173091799435028 2314 76-77 -0.6814217769128739 2314 78-79 -0.19703175311139276 2314 80-81 0.3989673746463325 2314 82-83 0.12859635725765273 2314 84-85 0.10219577308425087 2314 86-87 -0.13913579625621608 2314 88-89 -0.037799162165384814 2314 90-91 0.26759310383900825 2314 92-93 0.15250376170645907 2314 94-95 0.16111207684744144 2314 96-97 0.46318400736989673 2314 98-99 0.06991352150514629 2314 100-101 0.05770562440160987 2315 1 -0.29499489336213713 2315 2 -0.2262755138530359 2315 3 -0.39977584225156093 2315 4 -0.2074428954858476 2315 5 -1.2420252744636784 2315 6 -0.42531886677338093 2315 7 -0.1873980716338437 2315 8 -0.23839614712021984 2315 9 0.03481040855552919 2315 10-11 -1.6340988646555843 2315 12-13 -1.2005091061405366 2315 14-15 -0.1958427554732367 2315 16-17 -1.7084883054078261 2315 18-19 -2.99781531872906 2315 20-21 -2.0296078846566274 2315 22-23 -1.2438978529415294 2315 24-25 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2315 90-91 -3.8515722047679795 2315 92-93 -4.843240089986981 2315 94-95 -2.654493478207119 2315 96-97 -1.5281265577976484 2315 98-99 -2.8617763449004308 2315 100-101 -2.147036365730184 2316 1 -0.44053128766090666 2316 2 -0.33942968641314764 2316 3 -0.9060523627607537 2316 4 -0.42946035822113515 2316 5 -0.36477443124748987 2316 6 -2.5103204988047167 2316 7 -0.5476621887048907 2316 8 -0.3814708941553633 2316 9 -0.08044500434839819 2316 10-11 0.010512711886683235 2316 12-13 -0.6099551675049142 2316 14-15 -1.427999212877225 2316 16-17 -1.3449703258626187 2316 18-19 -0.3542985632073581 2316 20-21 -3.971320565540097 2316 22-23 -4.312120162809876 2316 24-25 -2.9475756180102053 2316 26-27 -2.1856379953554566 2316 28-29 -1.5876496756075582 2316 30-31 -0.5707056775706363 2316 32-33 -2.075812521137948 2316 34-35 -2.326191476389141 2316 36-37 -4.756584935191249 2316 38-39 -2.53086269631342 2316 40-41 -1.8361528766582467 2316 42-43 -2.891641668185315 2316 44-45 -1.0499746895438022 2316 46-47 -1.0854722028188561 2316 48-49 -0.5089369436195099 2316 50-51 -0.40566910877787166 2316 52-53 -2.8343664590856292 2316 54-55 -1.0533797870530464 2316 56-57 0.08432545574472883 2316 58-59 0.09543981574736549 2316 60-61 -1.530026698396302 2316 62-63 -0.8921115509196831 2316 64-65 -0.1397922546371717 2316 66-67 1.1337485970237893 2316 68-69 3.791458783621472 2316 70-71 3.849151409949563 2316 72-73 2.158150945697571 2316 74-75 0.9545360792510102 2316 76-77 -0.012409625900676957 2316 78-79 0.25135814178936045 2316 80-81 -0.38718588221600925 2316 82-83 -0.2086779374005978 2316 84-85 0.10203103932347446 2316 86-87 -0.46303763814930576 2316 88-89 0.1517205563318491 2316 90-91 0.41735595865275954 2316 92-93 0.6501637833673861 2316 94-95 1.232025942965766 2316 96-97 1.400145665029033 2316 98-99 0.2813868038320244 2316 100-101 0.22832404118963368 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 30.0 4 85.0 5 194.0 6 488.0 7 1255.0 8 2627.0 9 4249.0 10 6604.0 11 9641.0 12 12659.0 13 15960.0 14 19566.0 15 24498.0 16 29096.0 17 33225.0 18 31044.0 19 27227.0 20 26425.0 21 26685.0 22 28319.0 23 32193.0 24 38637.0 25 48073.0 26 59829.0 27 74670.0 28 90004.0 29 107013.0 30 124312.0 31 144501.0 32 166611.0 33 191321.0 34 220158.0 35 247979.0 36 255691.0 37 200242.0 38 72536.0 39 3355.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.11518109805489 20.392934914105975 15.646577388779601 22.84530659905953 2 16.2707101886386 21.385689183912536 38.03307066205281 24.31052996539606 3 19.425707085792656 24.81857459581575 30.381866294209953 25.37385202418164 4 13.771586993744242 17.839512665070824 35.654877345909355 32.73402299527557 5 13.88159796180316 37.55598962222597 33.527471357840106 15.034941058130762 6 32.10419813126575 36.1889095965099 16.68634124383154 15.020551028392811 7 28.23900614637717 33.1314550632938 20.156541200920483 18.472997589408543 8 26.810278459072535 34.648150407444646 19.157470940383085 19.384100193099734 9 26.332114715546002 16.704809824106757 20.129784897833833 36.83329056251341 10-11 25.192721107609984 26.93743400322254 26.42277483056445 21.447070058603035 12-13 26.106326856008177 24.280019856879022 27.3394937337243 22.2741595533885 14-15 23.0270802394605 25.464259721246442 26.048375900816573 25.460284138476492 16-17 22.151166239447303 28.7763267885262 26.624012042074902 22.44849492995159 18-19 21.95130673082647 26.73466326936074 29.646255134926374 21.66777486488641 20-21 22.799109553411544 25.82648491728788 29.701290634303312 21.673114894997266 22-23 22.530300671852455 25.853404066453233 29.683762373738436 21.932532887955876 24-25 22.501377781330326 26.058725036916126 29.63744367924409 21.802453502509454 26-27 22.156228728457105 26.33220333849109 29.58415684967871 21.927411083373087 28-29 21.918015432013053 26.520606172300305 29.757327429145114 21.80405096654153 30-31 22.0862208527424 26.103708365868762 29.677108295861494 22.132962485527344 32-33 22.000663498116058 26.23270439854653 29.5468291037958 22.21980299954161 34-35 22.59468429172906 26.257411854001678 29.322436738645397 21.82546711562387 36-37 22.487128502720225 25.961437834316232 29.42614526372488 22.12528839923867 38-39 22.17427220928012 26.34003428563968 29.547748365051728 21.93794514002847 40-41 22.289653963964266 26.114764911831408 29.608169537102246 21.98741158710208 42-43 22.288712389758818 26.18468337760318 29.12835082437934 22.398253408258658 44-45 22.27932012297772 26.411214799775877 28.97358750076066 22.335877576485743 46-47 22.426733873913975 26.630843670925657 28.533481459925696 22.408940995234666 48-49 22.618944839781964 26.693052761380052 28.30980470275003 22.37819769608795 50-51 22.368487270652224 26.911971093257304 28.22069186250346 22.49884977358701 52-53 22.783277883176584 26.954754861467233 27.834799794198663 22.427167461157524 54-55 22.344643631823928 26.86924715674382 28.074917019291266 22.71119219214099 56-57 22.520605158696757 27.159838121563702 27.901051469127612 22.418505250611926 58-59 22.65906745169354 26.791398309131893 27.96149115834942 22.588043080825145 60-61 22.625534202081166 26.746339867699852 28.34138865719241 22.286737273026567 62-63 22.5325631884989 26.761544336862848 28.247205301066845 22.458687173571406 64-65 22.360002027850975 26.748511349128833 28.132687926987266 22.758798696032922 66-67 22.02572758291592 26.90588871889669 28.55937917093907 22.509004527248322 68-69 22.042405765554758 26.367462888590453 29.230233944555117 22.35989740129967 70-71 21.694867311362046 26.450660162234225 29.411589821520266 22.442882704883463 72-73 22.39982059435083 26.744922465705333 28.15607531999352 22.699181619950313 74-75 22.05279352294956 27.114959007543288 27.94772263544154 22.884524834065612 76-77 22.237874430161806 27.20673516190673 27.657577656725625 22.89781275120584 78-79 22.210184615054505 27.230525950638473 27.624499302742056 22.93479013156497 80-81 22.096226704426055 27.3824501294815 27.5675799612155 22.953743204876943 82-83 22.284256271534407 27.289662222708362 27.44632963260567 22.979751873151564 84-85 22.129145439017925 27.178324870320274 27.385791393389177 23.306738297272624 86-87 22.335336205998942 27.08343806958554 27.530029943174227 23.05119578124129 88-89 22.08417192821852 27.16018817059544 27.351627603290208 23.40401229789583 90-91 21.981629228988 27.318601993834058 27.512213243312377 23.187555533865563 92-93 22.20602282439294 27.115604311278897 27.45305008530802 23.225322779020136 94-95 21.677647419986826 26.875035316362926 27.876122427809136 23.571194835841112 96-97 21.695627035600527 26.806921642743063 27.877168337600462 23.620282984055947 98-99 21.863549544648322 26.95184605244418 27.210315905026317 23.974288497881183 100-101 21.696102845925065 26.78871121578804 27.615524329182016 23.899661609104875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2070.0 1 1430.0 2 796.5 3 868.0 4 883.5 5 856.5 6 1303.0 7 2626.5 8 3983.0 9 4717.5 10 4600.0 11 4070.5 12 3873.5 13 3861.0 14 4101.5 15 4303.5 16 4369.5 17 4629.0 18 4920.0 19 5271.0 20 5684.5 21 6254.5 22 7202.5 23 8397.0 24 9782.0 25 11488.0 26 13920.0 27 16646.5 28 19236.0 29 22053.5 30 25307.0 31 29255.0 32 33641.0 33 37591.0 34 41362.0 35 45121.0 36 49865.0 37 54763.5 38 57799.0 39 59559.5 40 61722.5 41 66229.5 42 71336.0 43 75273.5 44 78724.5 45 81583.5 46 83665.5 47 87048.5 48 92968.0 49 95778.5 50 94191.0 51 92370.5 52 90356.5 53 88875.0 54 86453.0 55 80167.0 56 72323.5 57 65116.0 58 58173.5 59 50923.0 60 42446.5 61 33916.0 62 26769.5 63 20607.5 64 15657.0 65 12078.5 66 9235.0 67 6937.5 68 4931.5 69 3390.5 70 2514.0 71 1967.5 72 1638.0 73 1306.5 74 994.5 75 752.0 76 505.0 77 285.0 78 151.0 79 84.5 80 53.0 81 30.0 82 20.0 83 15.5 84 7.5 85 3.0 86 2.5 87 1.5 88 1.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022507267533582107 2 0.016912002894392535 3 0.0 4 0.0 5 2.9448761258892475E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.627662483540246E-4 18-19 0.0011989852798263364 20-21 2.1034829470637482E-5 22-23 0.0 24-25 0.0 26-27 3.365572715301997E-4 28-29 0.016491306304979785 30-31 0.004795941119305346 32-33 0.008981872183962205 34-35 0.010222927122729816 36-37 0.10435378900383253 38-39 0.1071303864939567 40-41 0.12292754342640544 42-43 0.10757211791284009 44-45 0.10237651503359263 46-47 0.10368067446077216 48-49 0.10631002814460183 50-51 0.10422758002700873 52-53 0.08010063062418753 54-55 0.06766904640704079 56-57 0.03811511100079512 58-59 0.015187146877800262 60-61 0.009150150819727304 62-63 0.0022927964122994855 64-65 0.004606627654069609 66-67 0.4443187029082756 68-69 0.8521209418555244 70-71 0.9471142317449232 72-73 0.2928889655491563 74-75 0.15597326052477692 76-77 0.014661276141034324 78-79 0.008392896958784356 80-81 0.0012410549387676115 82-83 0.0 84-85 0.0 86-87 3.1552244205956223E-4 88-89 7.572538609429494E-4 90-91 0.0034497120331845473 92-93 0.001493472892415261 94-95 0.23508525416384451 96-97 0.24320469833951056 98-99 0.013904022280091376 100-101 0.007278050996840569 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2377010.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.96504185151519 #Duplication Level Percentage of deduplicated Percentage of total 1 72.21439624163492 35.35980934253555 2 14.320040588088645 14.023627734223131 3 5.582790611136279 8.200847277676017 4 2.7440241555561102 5.374450304734943 5 1.4216153359600918 3.4804727211020854 6 0.8544908851624319 2.510410917223004 7 0.5667698969895879 1.9426338208391885 8 0.4016380715586906 1.5732979986426507 9 0.294391277988306 1.2973393122676602 >10 1.4126849140585733 12.467906668646119 >50 0.10966299951694913 3.7225940193551756 >100 0.0719328613285973 6.481657104009515 >500 0.004345438297563863 1.436270889611481 >1k 9.559964254639553E-4 0.7899468615405729 >5k 8.690876595126865E-5 0.3327368696000506 >10k+ 1.738175319025373E-4 1.0059981579927353 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12279 0.5165733421399153 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11361 0.47795339523182484 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7819 0.3289426632618289 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3078 0.12949041022124433 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2469 0.10386998792600789 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.1034829470637482E-5 0.0 22-23 0.0 0.0 0.0 1.4724380629446238E-4 0.0 24-25 0.0 0.0 0.0 5.889752251778495E-4 0.0 26-27 0.0 0.0 0.0 0.0014093335745327114 0.0 28-29 0.0 0.0 0.0 0.0036390254984202844 0.0 30-31 0.0 0.0 0.0 0.008834628377667741 0.0 32-33 0.0 0.0 0.0 0.018026848856336323 0.0 34-35 0.0 0.0 0.0 0.032793299144723835 0.0 36-37 0.0 0.0 0.0 0.05683610922966247 0.0 38-39 0.0 0.0 0.0 0.08962940837438631 0.0 40-41 0.0 0.0 0.0 0.1255989667691764 0.0 42-43 0.0 0.0 0.0 0.16232577902490947 0.0 44-45 0.0 0.0 0.0 0.20717203545630855 0.0 46-47 0.0 0.0 0.0 0.26463918957009014 0.0 48-49 0.0 0.0 0.0 0.32896369809129955 0.0 50-51 0.0 0.0 0.0 0.3955178985363966 0.0 52-53 0.0 0.0 0.0 0.45982137222813535 0.0 54-55 0.0 0.0 0.0 0.5317184193587743 0.0 56-57 0.0 0.0 0.0 0.6161522248539131 0.0 58-59 0.0 0.0 0.0 0.7006912044964051 0.0 60-61 0.0 2.1034829470637482E-5 0.0 0.7818015069351834 0.0 62-63 0.0 4.2069658941274964E-5 0.0 0.8639004463590814 2.1034829470637482E-5 64-65 0.0 4.2069658941274964E-5 0.0 0.9549391883080004 4.2069658941274964E-5 66-67 0.0 4.2069658941274964E-5 0.0 1.0495117816079864 4.2069658941274964E-5 68-69 0.0 4.2069658941274964E-5 0.0 1.1646985077891974 4.2069658941274964E-5 70-71 0.0 4.2069658941274964E-5 0.0 1.3139406228833703 4.2069658941274964E-5 72-73 0.0 4.2069658941274964E-5 0.0 1.4765188198619275 4.2069658941274964E-5 74-75 0.0 4.2069658941274964E-5 0.0 1.657523527456763 4.2069658941274964E-5 76-77 0.0 4.2069658941274964E-5 0.0 1.8615613733219465 4.2069658941274964E-5 78-79 0.0 4.2069658941274964E-5 0.0 2.095552816353318 4.2069658941274964E-5 80-81 0.0 4.2069658941274964E-5 0.0 2.3481600834662033 4.2069658941274964E-5 82-83 0.0 4.2069658941274964E-5 0.0 2.611432009120702 4.2069658941274964E-5 84-85 0.0 4.2069658941274964E-5 0.0 2.874640830286789 4.2069658941274964E-5 86-87 0.0 4.2069658941274964E-5 0.0 3.1513329771435545 4.2069658941274964E-5 88-89 0.0 4.2069658941274964E-5 0.0 3.4509110184643736 4.2069658941274964E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7625 0.0 42.946987 1 GTATCAA 12190 0.0 34.833637 1 TATCAAC 16140 0.0 26.30314 2 TCAACGC 16280 0.0 26.073654 4 ATCAACG 16265 0.0 26.03945 3 CAACGCA 16565 0.0 25.596462 5 AACGCAG 17055 0.0 24.943869 6 GTACATG 11940 0.0 22.663465 1 TACATGG 12045 0.0 21.9498 2 ACGCAGA 19415 0.0 21.6922 7 CGCAGAG 19585 0.0 21.47972 8 GAGTACT 10805 0.0 20.848026 12-13 AGTACTT 11195 0.0 20.798817 12-13 ACATGGG 12240 0.0 20.707169 3 GCAGAGT 20395 0.0 20.115616 9 AGAGTAC 17790 0.0 19.98545 10-11 GTACTTT 11275 0.0 19.957964 14-15 GTGGTAT 4370 0.0 19.52026 1 TGGTATC 4370 0.0 18.974037 2 TACTTTT 12610 0.0 18.201948 14-15 >>END_MODULE