FastQCFastQC Report
Fri 27 May 2016
SRR939396_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939396_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2337676
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86290.36912728710052206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63320.2708673058199682No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45540.19480886145043197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69400.040.9990841
GTATCAA116800.039.388221
TATCAAC147050.031.087532
ATCAACG152000.030.2635443
TCAACGC155000.029.6738624
CAACGCA157450.029.2782255
AACGCAG161200.028.4263526
ACGCAGA176300.025.4987347
CGCAGAG179450.024.8629258
GTACATG105350.023.7332291
GCAGAGT176500.023.3861949
TACATGG106550.023.1690772
GAGTACT92850.022.38622712-13
ACATGGG112850.021.5526373
AGTACTT95850.021.08457612-13
AGAGTAC149000.021.01251210-11
GTACTTT101900.020.74931314-15
CAGAGTA173150.019.12982410-11
ACTTTTT124100.018.9019516-17
CATGGGA66150.018.8777354
Produced by FastQC (version 0.11.5)