FastQCFastQC Report
Fri 27 May 2016
SRR939396_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939396_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2337676
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117300.5017804007056581No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105400.4508751426630551No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74840.3201470178074292No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27250.11656876316478418No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24720.10574604863976017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81650.041.2144621
GTATCAA125100.032.363191
TATCAAC160500.025.1600672
ATCAACG163550.024.6565723
TCAACGC164050.024.5288334
CAACGCA165500.024.2056265
AACGCAG170500.023.5235886
GTACATG115500.021.903061
TACATGG117600.021.3051172
GAGTACT104550.021.28558212-13
ACGCAGA195650.020.4997277
CGCAGAG197000.020.3351828
AGTACTT108750.020.1802112-13
GTACTTT111600.019.96216614-15
ACATGGG118750.019.8557853
AGAGTAC173100.019.4573110-11
GCAGAGT203550.019.1218389
GTACGAC2001.2992436E-518.9768583
TACTTTT123900.017.61701414-15
ACTTTTT129850.017.61302616-17
Produced by FastQC (version 0.11.5)