##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939396_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2337676 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.7926222453411 31.0 30.0 31.0 28.0 34.0 2 31.063177275208368 31.0 31.0 34.0 28.0 34.0 3 31.052260450122258 31.0 31.0 34.0 27.0 34.0 4 34.98220839842647 35.0 35.0 37.0 32.0 37.0 5 34.531463727223105 35.0 35.0 37.0 30.0 37.0 6 34.59649711936128 35.0 35.0 37.0 30.0 37.0 7 34.4331511295834 35.0 35.0 37.0 30.0 37.0 8 34.34089240767326 35.0 35.0 37.0 30.0 37.0 9 35.829951199396326 37.0 35.0 39.0 30.0 39.0 10-11 35.60976970290152 37.0 35.0 39.0 30.0 39.0 12-13 35.58815764032312 37.0 35.0 39.0 30.0 39.0 14-15 36.38988251579774 38.0 35.0 40.0 30.0 41.0 16-17 36.300326905867195 38.0 34.5 40.0 30.0 41.0 18-19 36.296944914521944 38.0 34.0 40.0 30.0 41.0 20-21 36.17005564500812 38.0 34.0 40.0 29.5 41.0 22-23 36.00287379431538 38.0 34.0 40.0 29.0 41.0 24-25 35.82303214816767 38.0 34.0 40.0 28.0 41.0 26-27 35.334792759988986 38.0 33.5 40.0 27.0 41.0 28-29 35.10373208263249 37.5 33.0 40.0 27.0 41.0 30-31 34.79081275591656 37.0 33.0 39.5 26.0 40.5 32-33 34.561289930683294 37.0 33.0 40.0 25.0 41.0 34-35 34.78731291248231 37.0 33.0 40.0 26.0 41.0 36-37 34.62967815043659 37.0 33.0 40.0 25.0 41.0 38-39 34.44845992344534 37.0 33.0 40.0 25.0 41.0 40-41 34.275152116888734 37.0 33.0 40.0 24.5 41.0 42-43 34.08014177328252 37.0 33.0 40.0 24.0 41.0 44-45 33.67304023312042 37.0 32.0 40.0 23.5 41.0 46-47 33.237040975738296 37.0 31.0 39.5 22.0 41.0 48-49 32.82301931490934 36.5 31.0 39.0 19.5 40.0 50-51 32.561904215982025 36.0 31.0 39.0 18.5 40.0 52-53 32.420100561412276 36.0 31.0 39.0 18.5 40.0 54-55 32.064578239242735 36.0 30.0 39.0 16.5 40.0 56-57 31.54565709704852 35.0 29.5 39.0 13.5 40.0 58-59 31.05721451561294 35.0 28.5 38.0 10.5 40.0 60-61 30.66592953856736 34.0 28.0 38.0 8.0 40.0 62-63 30.475273305624903 34.0 28.0 38.0 8.0 40.0 64-65 29.986347124237916 34.0 28.0 37.5 7.0 40.0 66-67 29.0486707738797 33.5 27.0 37.0 4.0 39.0 68-69 25.518575713657498 31.0 15.5 35.0 2.0 38.5 70-71 24.15113043894877 29.0 11.5 35.0 2.0 38.0 72-73 25.18877936891169 30.0 16.5 34.5 2.0 37.0 74-75 25.94887187103773 30.5 21.0 34.5 2.0 37.0 76-77 25.676919299338316 30.5 21.0 34.0 2.0 36.0 78-79 26.83811144059314 31.5 24.5 34.5 2.0 36.0 80-81 26.850483771061516 32.0 25.0 35.0 2.0 36.0 82-83 26.51602681466551 32.0 24.5 34.5 2.0 36.0 84-85 26.146287167255 31.5 23.5 34.0 2.0 35.5 86-87 25.68623153935789 31.0 22.5 34.0 2.0 35.0 88-89 25.183760709354075 31.0 20.0 34.0 2.0 35.0 90-91 24.560518437970018 31.0 18.5 34.0 2.0 35.0 92-93 23.94639826049461 30.5 14.5 34.0 2.0 35.0 94-95 23.04741183123752 30.0 4.5 34.0 2.0 35.0 96-97 22.343720002258653 29.5 2.0 34.0 2.0 35.0 98-99 21.456588509271604 29.0 2.0 34.0 2.0 35.0 100-101 20.04436072406955 27.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2648305795056629 1101 2 0.197512169185881 1101 3 0.33950675435162125 1101 4 0.37554406995324996 1101 5 0.4346546267748863 1101 6 0.3709635693641573 1101 7 0.44638448834106725 1101 8 0.7229619492533104 1101 9 0.6358371180751661 1101 10-11 0.755479884734207 1101 12-13 0.6588968109244178 1101 14-15 0.7115607893201528 1101 16-17 0.7221227654170761 1101 18-19 0.5131966439345845 1101 20-21 0.7152810511716154 1101 22-23 0.6636967362454911 1101 24-25 0.7352407836696884 1101 26-27 0.6882533989066388 1101 28-29 0.6266023252101149 1101 30-31 0.4594171475491464 1101 32-33 0.7465617115423626 1101 34-35 0.7315998343663281 1101 36-37 0.828128198922542 1101 38-39 0.9019565903676892 1101 40-41 1.0014323738884556 1101 42-43 0.5271539478626508 1101 44-45 0.7699418553387218 1101 46-47 0.5938958595060129 1101 48-49 0.7342953719024194 1101 50-51 0.40839927217469807 1101 52-53 0.30244265863392883 1101 54-55 0.24007665511949838 1101 56-57 0.4804995891888417 1101 58-59 0.5181040354045727 1101 60-61 0.3494219143779098 1101 62-63 -0.05013966541217485 1101 64-65 -0.1286464191606811 1101 66-67 -0.452069075325646 1101 68-69 -6.8591817515674585 1101 70-71 -7.772736040794491 1101 72-73 -5.409902033614362 1101 74-75 -3.3867838557637846 1101 76-77 -0.5741191437495097 1101 78-79 -0.23023615405199038 1101 80-81 -0.23224323069759478 1101 82-83 -0.5596051489636054 1101 84-85 -0.5894654711846279 1101 86-87 -0.6280017043748387 1101 88-89 -0.6664130318875401 1101 90-91 -0.6210567605790587 1101 92-93 -0.7140580993789882 1101 94-95 -0.5567581696075514 1101 96-97 -0.9732608333498263 1101 98-99 -1.03174678970219 1101 100-101 -0.8079978068668972 1102 1 0.32661432182237604 1102 2 0.33806036600897826 1102 3 0.3775397535501632 1102 4 0.28810889150457086 1102 5 0.5300907988636325 1102 6 0.5207503221674159 1102 7 0.4638115994038401 1102 8 0.6064654386089003 1102 9 0.5577614008011409 1102 10-11 0.653157528255008 1102 12-13 0.6475786460012785 1102 14-15 0.7196763525113283 1102 16-17 0.7249497822899471 1102 18-19 0.5818539653512715 1102 20-21 0.6742933982846253 1102 22-23 0.6100837960106702 1102 24-25 0.5801293185838361 1102 26-27 0.7447386954622672 1102 28-29 0.6952649923259031 1102 30-31 0.6380143146717998 1102 32-33 0.7429067052753524 1102 34-35 0.6715060589247699 1102 36-37 0.8739931092784587 1102 38-39 0.8415288077267178 1102 40-41 0.7456052318866213 1102 42-43 0.6524562768451503 1102 44-45 0.6501570797841723 1102 46-47 0.8491426301757841 1102 48-49 0.9188095019710616 1102 50-51 0.9295939131707556 1102 52-53 0.6700088370688775 1102 54-55 0.3685633708592455 1102 56-57 0.5828330330439542 1102 58-59 0.6221036874721584 1102 60-61 0.8994890062014456 1102 62-63 0.5529089141893735 1102 64-65 0.37854875983708425 1102 66-67 0.4975141845318234 1102 68-69 -6.474728377678968 1102 70-71 -7.408117124464056 1102 72-73 -1.3746354007025943 1102 74-75 0.4683449927435923 1102 76-77 0.9204782023550706 1102 78-79 0.6089348586900485 1102 80-81 0.49801140672937194 1102 82-83 0.33255738299179427 1102 84-85 0.07154870164961125 1102 86-87 -0.08302154021832209 1102 88-89 0.1487305813087616 1102 90-91 0.05057614854775494 1102 92-93 0.031362484387187806 1102 94-95 0.1299248623779441 1102 96-97 -0.21201724422897428 1102 98-99 -0.426358311808837 1102 100-101 -0.3182462380032014 1103 1 0.3955860836777738 1103 2 0.21141816261773627 1103 3 0.38329403527384187 1103 4 0.3135017037456791 1103 5 0.44418325349651155 1103 6 0.4128721431384932 1103 7 0.3261734112994148 1103 8 0.43410810722389215 1103 9 0.5146259488784253 1103 10-11 0.5786505015030841 1103 12-13 0.5241461742165257 1103 14-15 0.7089678802353276 1103 16-17 0.645037651814981 1103 18-19 0.5910472731025109 1103 20-21 0.5368839842167503 1103 22-23 0.6053588998794197 1103 24-25 0.7209572685395003 1103 26-27 0.7399508328063078 1103 28-29 0.7161498437249065 1103 30-31 0.5465911489230919 1103 32-33 0.751851204186714 1103 34-35 0.6939000253786958 1103 36-37 0.9415245118812194 1103 38-39 0.8847015972374308 1103 40-41 0.9374945450623429 1103 42-43 0.7898713500944794 1103 44-45 0.9126157352355122 1103 46-47 0.8805856277778474 1103 48-49 0.8668012991501612 1103 50-51 0.5894070509019542 1103 52-53 0.6418613694680175 1103 54-55 0.38992606532693586 1103 56-57 0.8138795236749559 1103 58-59 0.9240629091018349 1103 60-61 0.8513744503183993 1103 62-63 0.4164907675491847 1103 64-65 0.39653181715847907 1103 66-67 0.4505884470429038 1103 68-69 -6.704082642958497 1103 70-71 -7.687099353853707 1103 72-73 -0.9253401053947989 1103 74-75 0.5444181817014488 1103 76-77 0.9797460291669431 1103 78-79 0.691946204112952 1103 80-81 0.43245466816638256 1103 82-83 0.2843124057430053 1103 84-85 0.05581650244225855 1103 86-87 0.168970686798378 1103 88-89 0.3841481716579196 1103 90-91 0.20464131421736198 1103 92-93 0.21979182605916492 1103 94-95 0.5791396932321646 1103 96-97 0.4451012775043637 1103 98-99 0.5964775911831666 1103 100-101 0.5358401593540769 1104 1 0.2404136698846706 1104 2 0.3138870271550971 1104 3 0.22679781890263584 1104 4 0.19586248241274973 1104 5 0.47657083546474155 1104 6 0.33590225633569304 1104 7 0.46989598466566207 1104 8 0.4281163227473641 1104 9 0.5174356205068165 1104 10-11 0.5439881451812383 1104 12-13 0.44280672599053617 1104 14-15 0.6571066091355249 1104 16-17 0.5690288659421938 1104 18-19 0.5773343458080191 1104 20-21 0.4700942872810572 1104 22-23 0.6220022694907072 1104 24-25 0.5589768941659514 1104 26-27 0.6497082473474052 1104 28-29 0.4606099753727477 1104 30-31 0.6970367538050368 1104 32-33 0.8702056996613194 1104 34-35 0.7203558186317451 1104 36-37 1.0413991441149903 1104 38-39 0.9542086346623222 1104 40-41 0.8777785042215882 1104 42-43 0.762854557514622 1104 44-45 0.8777644312237385 1104 46-47 0.9164299785146284 1104 48-49 1.028173924253018 1104 50-51 0.7956946846067936 1104 52-53 0.7390724236480821 1104 54-55 0.7455536677404844 1104 56-57 0.735378965792286 1104 58-59 0.8116612398171732 1104 60-61 0.9978455308031009 1104 62-63 0.7956320334129998 1104 64-65 0.6206315690721063 1104 66-67 0.6284801648228466 1104 68-69 -5.918016619571059 1104 70-71 -4.108075196599362 1104 72-73 0.6953713664181045 1104 74-75 1.318795160782404 1104 76-77 1.5179193062756795 1104 78-79 1.0705576372792294 1104 80-81 0.899665815846344 1104 82-83 0.5657135638949526 1104 84-85 0.7572195461752749 1104 86-87 0.8967991363209045 1104 88-89 0.9563795620635851 1104 90-91 0.7760089994094734 1104 92-93 0.8349155108274076 1104 94-95 1.0008744434004662 1104 96-97 0.7132254515886913 1104 98-99 0.8288505248356444 1104 100-101 1.051089211888545 1105 1 0.13384432681897707 1105 2 0.22850799817441114 1105 3 0.23746647004185917 1105 4 0.27381873957083513 1105 5 0.38306602470214557 1105 6 0.2419973843500074 1105 7 0.28042345324924156 1105 8 0.40577833962593957 1105 9 0.4250871872373452 1105 10-11 0.43711466586250936 1105 12-13 0.31410286101911566 1105 14-15 0.4654008863600083 1105 16-17 0.47716309228817266 1105 18-19 0.5622573683286163 1105 20-21 0.5202867969527247 1105 22-23 0.4887847850207905 1105 24-25 0.4736811233091984 1105 26-27 0.6609385939439889 1105 28-29 0.5886716576354729 1105 30-31 0.5395926066796193 1105 32-33 0.5979220784211279 1105 34-35 0.5486602821116477 1105 36-37 0.6521721647883396 1105 38-39 0.6316660028083732 1105 40-41 0.8405018233575063 1105 42-43 0.6627413475774873 1105 44-45 0.7488577647681822 1105 46-47 0.8688540961896294 1105 48-49 0.8558867404623598 1105 50-51 0.5607377518835861 1105 52-53 0.5717878523944364 1105 54-55 0.6573693281845614 1105 56-57 0.7794547082541925 1105 58-59 0.8178233965588362 1105 60-61 0.8575811895582639 1105 62-63 0.6640173008153987 1105 64-65 0.7976639381417847 1105 66-67 0.8818360873650803 1105 68-69 -2.6875502157414495 1105 70-71 -3.1386087611026348 1105 72-73 0.8950789733869797 1105 74-75 1.328436345976975 1105 76-77 1.4688749934256897 1105 78-79 1.3465391859067282 1105 80-81 1.1067341798703865 1105 82-83 0.8368194323391975 1105 84-85 0.9412292363125516 1105 86-87 0.803229276025661 1105 88-89 0.6677014794018561 1105 90-91 0.7574745007262997 1105 92-93 0.7443054702407252 1105 94-95 0.9545615511900465 1105 96-97 0.8884464616641097 1105 98-99 1.1062650104297482 1105 100-101 0.9256793866382438 1106 1 0.03423198876895128 1106 2 0.11237270442900282 1106 3 0.15219762414092486 1106 4 0.16105162012748053 1106 5 0.1462922030266398 1106 6 0.26076487247114954 1106 7 0.3108092442969763 1106 8 0.4180654901042544 1106 9 0.3403802998408949 1106 10-11 0.3858338544412021 1106 12-13 0.23122780702432522 1106 14-15 0.3189853837338674 1106 16-17 0.3330118628577807 1106 18-19 0.2774541844932088 1106 20-21 0.3391627561340371 1106 22-23 0.3410388095241075 1106 24-25 0.3992262987200448 1106 26-27 0.49666147755460344 1106 28-29 0.544230232007962 1106 30-31 0.5445989209746855 1106 32-33 0.5829915906934175 1106 34-35 0.47046640436410314 1106 36-37 0.5533093569492564 1106 38-39 0.6435929358218146 1106 40-41 0.5466713986351763 1106 42-43 0.4821918525835258 1106 44-45 0.6937915403314534 1106 46-47 0.7844901616822142 1106 48-49 0.8525663704639257 1106 50-51 0.6781011691415557 1106 52-53 0.4643377408281282 1106 54-55 0.268935974949958 1106 56-57 0.3004175488340657 1106 58-59 0.6190770386277258 1106 60-61 0.7579625922397071 1106 62-63 0.392509972656395 1106 64-65 0.29853266958751945 1106 66-67 0.4486483450763643 1106 68-69 -3.372081568486312 1106 70-71 -3.674187612753844 1106 72-73 0.6739148044665164 1106 74-75 1.2460850758269437 1106 76-77 1.2362048444963705 1106 78-79 0.991509347767284 1106 80-81 0.6819225769748911 1106 82-83 0.5746772767846835 1106 84-85 0.4553346011247328 1106 86-87 0.3807273432185667 1106 88-89 0.5913717631461921 1106 90-91 0.7497013146072931 1106 92-93 0.8211258856360502 1106 94-95 1.1510687417827086 1106 96-97 1.0913449844767342 1106 98-99 1.0063574550111483 1106 100-101 0.9734356108273587 1107 1 0.05193404606021801 1107 2 -0.003674575104312794 1107 3 0.1250325410209676 1107 4 -0.11972060555421393 1107 5 0.16900692120810845 1107 6 0.17322350155019706 1107 7 0.06582205288771092 1107 8 -7.795625775770532E-4 1107 9 0.11670275138625641 1107 10-11 0.0063052820673448196 1107 12-13 0.1342885462123462 1107 14-15 -0.07662511871973976 1107 16-17 -0.01929424473674146 1107 18-19 0.16247817696875444 1107 20-21 0.10197998735154812 1107 22-23 0.3569099709347938 1107 24-25 0.19161753499718515 1107 26-27 0.44513304283764654 1107 28-29 0.4437491122724495 1107 30-31 0.2833499002292612 1107 32-33 0.11016494504355734 1107 34-35 0.1025990849398255 1107 36-37 0.2361463770849781 1107 38-39 0.32452004028863257 1107 40-41 0.0964221835158483 1107 42-43 -0.1294076467707299 1107 44-45 0.055608159477934294 1107 46-47 0.20459620771515574 1107 48-49 -0.004067953723399853 1107 50-51 -0.07453299611999142 1107 52-53 -0.25171882906907683 1107 54-55 -0.07856270165321178 1107 56-57 0.2838213993280334 1107 58-59 0.3155532434800996 1107 60-61 0.1997776634845394 1107 62-63 -0.041156829107031 1107 64-65 -0.048576594544762486 1107 66-67 -0.02820269715772028 1107 68-69 -3.4504906889355063 1107 70-71 -3.3011765482110746 1107 72-73 0.6575360701537925 1107 74-75 0.8985848483681131 1107 76-77 0.6825771420196105 1107 78-79 0.6478957861401184 1107 80-81 0.34193742678707295 1107 82-83 0.24143753635951626 1107 84-85 0.09958936033149612 1107 86-87 0.27658236391436475 1107 88-89 0.24277212672583914 1107 90-91 0.46923770920168906 1107 92-93 0.23577602146146148 1107 94-95 0.5587471906198331 1107 96-97 0.47452264636539 1107 98-99 0.5824036622385478 1107 100-101 0.6578033254982749 1108 1 -0.0690724961232334 1108 2 -0.03265440337199621 1108 3 -0.010922208876809947 1108 4 -0.01454137698667779 1108 5 -0.05541382296526365 1108 6 -0.16407716629769453 1108 7 0.1134879926451049 1108 8 0.07693612503316416 1108 9 0.16060693565776063 1108 10-11 0.08753228220364662 1108 12-13 0.07644814822952384 1108 14-15 -0.06920212567080597 1108 16-17 0.22088225792102634 1108 18-19 0.047410710701889514 1108 20-21 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3.646059738207992 2311 70-71 5.0402729570445075 2311 72-73 3.856267648902577 2311 74-75 2.845351873581688 2311 76-77 2.431516750814648 2311 78-79 1.8120561748296993 2311 80-81 1.401104151097936 2311 82-83 1.4029719043537838 2311 84-85 1.309684968472535 2311 86-87 1.4152110241898619 2311 88-89 1.38979568171683 2311 90-91 1.6317945411395094 2311 92-93 1.845763310916034 2311 94-95 1.7842681893527086 2311 96-97 1.733545993837275 2311 98-99 1.8859123465988397 2311 100-101 2.14656997812072 2312 1 -0.19672160862991817 2312 2 -0.018185064614801405 2312 3 -0.05799133165807646 2312 4 -0.02536323878305069 2312 5 -1.0930680727634368 2312 6 -0.23587343599501764 2312 7 0.024918990432922783 2312 8 0.16654621509302814 2312 9 -2.6639881094770175 2312 10-11 -0.12104280088419017 2312 12-13 -1.0619557267560609 2312 14-15 -3.873481279800039 2312 16-17 -0.926787613437007 2312 18-19 -0.16137038825912242 2312 20-21 0.16068206113983052 2312 22-23 0.19038597768667387 2312 24-25 0.3985020298127111 2312 26-27 0.43569851185139186 2312 28-29 0.30904330084250375 2312 30-31 0.08445310736694722 2312 32-33 0.6310233969364916 2312 34-35 0.4015591765723272 2312 36-37 0.4920301484859948 2312 38-39 0.5423220658492767 2312 40-41 0.24953588016402506 2312 42-43 0.0706737373623767 2312 44-45 0.6338966813009321 2312 46-47 0.833511688785805 2312 48-49 1.0510381470723544 2312 50-51 1.089962422254743 2312 52-53 1.1377886462142612 2312 54-55 0.9654841997212777 2312 56-57 1.0553279100586792 2312 58-59 1.0787103449992692 2312 60-61 1.0896287606632242 2312 62-63 1.0862244103431706 2312 64-65 1.1526049824988824 2312 66-67 1.5420589377590872 2312 68-69 4.123415146608259 2312 70-71 4.908764372936744 2312 72-73 2.989712945003081 2312 74-75 2.5424177396147982 2312 76-77 2.452483098132465 2312 78-79 1.7656586267477863 2312 80-81 1.5383233684316373 2312 82-83 1.6169439265210812 2312 84-85 1.4443415908595902 2312 86-87 1.3917077557638962 2312 88-89 1.5046029682980624 2312 90-91 1.6022043115648437 2312 92-93 1.7558357031590752 2312 94-95 1.9204001927900798 2312 96-97 1.9874144958951696 2312 98-99 2.0758597750912138 2312 100-101 1.8230583291153515 2313 1 -0.20273353823715823 2313 2 -0.20772713465748538 2313 3 -0.13066499092371942 2313 4 0.0959740621096472 2313 5 0.07665415319894464 2313 6 0.15315563400727683 2313 7 -2.924621194570239 2313 8 -3.0628644407660737 2313 9 -0.9758409686780283 2313 10-11 -0.07208630490335821 2313 12-13 -0.016511463335255883 2313 14-15 -0.6276620281607208 2313 16-17 -0.3520747453493769 2313 18-19 0.15495068782914956 2313 20-21 -2.451598971323243 2313 22-23 -0.49407860376723534 2313 24-25 0.06744061791811617 2313 26-27 -1.2299123082754377 2313 28-29 -1.6331492694088823 2313 30-31 -1.097828194387482 2313 32-33 -1.2302134582790103 2313 34-35 -1.543385551376069 2313 36-37 -2.271303139965447 2313 38-39 -2.1349046370819593 2313 40-41 -1.141216654540905 2313 42-43 -0.08714379081222745 2313 44-45 -0.11562869267743991 2313 46-47 -0.28282621801245256 2313 48-49 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2314 8 -0.07992253684094663 2314 9 -0.07927197803983432 2314 10-11 -0.1486777980272933 2314 12-13 -2.2004515191370118 2314 14-15 -0.5025329211816469 2314 16-17 -1.9223079779677903 2314 18-19 -0.3692405860539125 2314 20-21 -0.5300547003679767 2314 22-23 -0.3947175972423409 2314 24-25 -0.24879284606000596 2314 26-27 -1.7602885983123784 2314 28-29 -0.8221844718899405 2314 30-31 -0.05504799437150609 2314 32-33 0.18055413263296316 2314 34-35 -0.010015769708026312 2314 36-37 -0.3506834815515276 2314 38-39 -0.2512833894471953 2314 40-41 -0.40689471750929584 2314 42-43 -0.5343575589181953 2314 44-45 -3.8529742807304643 2314 46-47 -3.9478977880359487 2314 48-49 -3.733152373803101 2314 50-51 -4.231847825424122 2314 52-53 -1.291459451469862 2314 54-55 -1.7028677693753558 2314 56-57 -1.3167113927157388 2314 58-59 -1.8275720828839965 2314 60-61 -1.0792095277346299 2314 62-63 -1.3721189377039948 2314 64-65 -1.2229991746028297 2314 66-67 -1.1185732341552885 2314 68-69 -1.6921798857098835 2314 70-71 1.2838131272022935 2314 72-73 1.0626035584216957 2314 74-75 0.7216969968516587 2314 76-77 0.8087276020335246 2314 78-79 -1.465673922679322 2314 80-81 -0.9114900340619876 2314 82-83 0.2573672934510789 2314 84-85 0.15107334956807605 2314 86-87 -0.12858898680350705 2314 88-89 -0.09230036224794347 2314 90-91 0.2901974492670405 2314 92-93 -0.2545520137884125 2314 94-95 0.37981908512194806 2314 96-97 0.6911134874194858 2314 98-99 0.28767147640279234 2314 100-101 0.5087052172619089 2315 1 -0.4117096576313273 2315 2 -0.3441129450832143 2315 3 -0.43174666100105696 2315 4 -0.13283149418414553 2315 5 -0.3282291604971945 2315 6 -0.14394780530787443 2315 7 -1.0427904760849884 2315 8 -3.0497500400550024 2315 9 -0.7731478670931722 2315 10-11 -1.4078734022300452 2315 12-13 -0.7067675737524155 2315 14-15 -0.48180562480970224 2315 16-17 -0.24039061191091804 2315 18-19 -0.13521166603612755 2315 20-21 -0.7268165308180485 2315 22-23 -0.3663665293998335 2315 24-25 -5.259204551183274 2315 26-27 -1.0053373133693029 2315 28-29 -0.9489910260670769 2315 30-31 -1.1438729967309555 2315 32-33 -0.3102168837152348 2315 34-35 -2.0936957600619124 2315 36-37 -3.6013397862271432 2315 38-39 -1.079371966983949 2315 40-41 -0.049753077853942784 2315 42-43 -1.614540045865489 2315 44-45 -5.246880187614586 2315 46-47 -3.2066120337535153 2315 48-49 -2.365783365650941 2315 50-51 -1.051080038123338 2315 52-53 0.01484473489699667 2315 54-55 -1.0115271402256134 2315 56-57 -1.0579018222358627 2315 58-59 -0.5920870929041442 2315 60-61 -1.7456443532161359 2315 62-63 -0.413092014140247 2315 64-65 0.05745574546483212 2315 66-67 0.44236801264840864 2315 68-69 3.2002522750973093 2315 70-71 3.773506689655953 2315 72-73 -2.1868569255187644 2315 74-75 -1.8276016875087997 2315 76-77 -0.4344981058554218 2315 78-79 -0.4440898082037492 2315 80-81 -0.56069617133533 2315 82-83 -1.2562403163688884 2315 84-85 -0.7290690189113711 2315 86-87 -0.308236076857316 2315 88-89 -0.2788177793761619 2315 90-91 -0.11297831728451513 2315 92-93 -1.1077338880689354 2315 94-95 0.22399166460686004 2315 96-97 0.10431887093278291 2315 98-99 -2.2854348829898683 2315 100-101 -3.5309620361886402 2316 1 -0.46492581594262106 2316 2 -0.4215786114902649 2316 3 -0.8672162258283045 2316 4 -0.5141273603478496 2316 5 -0.15019184595113444 2316 6 -0.5589531567198165 2316 7 -0.7921917736921387 2316 8 -0.9939055350979942 2316 9 -5.128838636396509 2316 10-11 -1.4355218526214415 2316 12-13 -0.5632297056583013 2316 14-15 -0.33378702774245284 2316 16-17 -0.37486501735966016 2316 18-19 -0.4725195396101256 2316 20-21 -3.9291294298491195 2316 22-23 -1.3386074460922899 2316 24-25 -0.5687880460559995 2316 26-27 -0.6588672319994586 2316 28-29 -0.0030360684277894734 2316 30-31 -0.07860360270405664 2316 32-33 -1.3433317889409793 2316 34-35 -0.7107396332033531 2316 36-37 -0.4678783334578185 2316 38-39 -5.61107069418772 2316 40-41 -5.408388370022635 2316 42-43 -3.924456781653756 2316 44-45 -2.4771396898700004 2316 46-47 -2.429407936539203 2316 48-49 -2.802413713276053 2316 50-51 -1.2143887944598042 2316 52-53 -0.9954059704118166 2316 54-55 -0.1814591076930192 2316 56-57 -3.8552566409800306 2316 58-59 -0.8368732530906335 2316 60-61 -0.9109076883172946 2316 62-63 -0.3337689802137156 2316 64-65 -0.05780016936968124 2316 66-67 -0.25019385070901023 2316 68-69 2.5719802155265654 2316 70-71 3.5650575944107885 2316 72-73 2.3385399437039247 2316 74-75 1.0936073519482292 2316 76-77 -1.6442527462064085 2316 78-79 -0.09392148127638933 2316 80-81 0.21465439312262902 2316 82-83 0.15990932835948257 2316 84-85 0.195141106167096 2316 86-87 0.3705862137003848 2316 88-89 -0.01318220381848434 2316 90-91 -0.13456534934275055 2316 92-93 0.0325064672141977 2316 94-95 0.5420676258956938 2316 96-97 0.8290120227744495 2316 98-99 0.6458095398265087 2316 100-101 0.8715590521324721 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 31.0 4 91.0 5 227.0 6 488.0 7 1339.0 8 2833.0 9 4670.0 10 7267.0 11 10341.0 12 13546.0 13 17044.0 14 20843.0 15 25997.0 16 30568.0 17 34338.0 18 32360.0 19 29100.0 20 29412.0 21 29562.0 22 31461.0 23 34902.0 24 40916.0 25 49859.0 26 62243.0 27 76981.0 28 92835.0 29 109785.0 30 127424.0 31 147245.0 32 168443.0 33 192024.0 34 216980.0 35 236177.0 36 228862.0 37 169628.0 38 59255.0 39 2590.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.77566053171134 20.57104031819162 15.798722236103933 22.854576913993107 2 16.23929246976263 21.57400747970805 37.91906495520176 24.26763509532756 3 19.39134188060147 24.932250829566403 30.402448252894875 25.273959036937256 4 13.760875205174376 17.89904007932125 35.829289781995 32.51079493350937 5 13.862656758250502 37.673270376219804 33.47957544159242 14.98449742393728 6 31.999472980857913 36.15462536296732 16.8133222910275 15.032579365147267 7 28.165793719916703 33.16105396984013 20.220466822605015 18.452685487638153 8 26.844010889447468 34.62507208013429 19.18247011134135 19.348446919076896 9 26.293849104837452 16.71677341085762 20.170074894895613 36.81930258940931 10-11 25.229261147115377 26.988760636838066 26.44836509117076 21.3336131248758 12-13 26.04037086405473 24.31228707485554 27.433185779380892 22.214156281708842 14-15 22.977521264709054 25.500026522067216 26.077523146920274 25.444929066303455 16-17 22.14005076445051 28.83174388742244 26.604623128389743 22.423582219737305 18-19 21.93635639528865 26.731208029862163 29.687116338719598 21.64531923612959 20-21 22.815886376041846 25.855550555337864 29.715452440800178 21.61311062782011 22-23 22.50688131919418 25.87414516893855 29.671217424019947 21.94775608784732 24-25 22.409216901731398 26.10627012063774 29.68313691904817 21.8013760585827 26-27 22.146693067172205 26.36280275147626 29.579118417903288 21.911385763448248 28-29 21.989331143094866 26.535124024392072 29.671464230026373 21.804080602486692 30-31 22.057535482438606 26.10284469090062 29.71436735844508 22.125252468215688 32-33 22.074733740036507 26.22324299651917 29.514912140735667 22.187111122708657 34-35 22.528135598440798 26.232263921068032 29.384032737738746 21.85556774275242 36-37 22.494984756786128 25.980918634569395 29.42990315887002 22.094193449774462 38-39 22.179470326563873 26.31464576895393 29.59537799959665 21.91050590488555 40-41 22.318556902071922 26.1126848177729 29.578164557842655 21.99059372231252 42-43 22.24090566483645 26.23940975258579 29.138777971557545 22.380906611020215 44-45 22.35979584873009 26.41462864325717 28.914501273483665 22.31107423452907 46-47 22.48741790411213 26.685431441036634 28.468898446094297 22.358252208756937 48-49 22.62757426576528 26.727776994110798 28.302705035287488 22.341943704836435 50-51 22.37265475231325 26.961411776390538 28.182785691474017 22.4831477798222 52-53 22.744343282285225 26.955501728593116 27.835699546246985 22.464455442874673 54-55 22.29567483878147 26.836264887369047 28.123766184714118 22.744294089135366 56-57 22.533198346265788 27.181630062977803 27.868938739776482 22.416232850979924 58-59 22.639068148896445 26.8176964766343 27.961287967911407 22.581947406557845 60-61 22.571873211793207 26.779393440255845 28.360106744246988 22.28862660370396 62-63 22.503060262193298 26.799234180481786 28.238215129748056 22.45949042757686 64-65 22.267861604224635 26.77460975868524 28.12779141041305 22.82973722667707 66-67 22.23211345508334 27.0563069678772 28.071854486181163 22.639725090858295 68-69 22.40468134937322 26.308923967731985 28.932516035613187 22.353878647281604 70-71 21.993611616969048 26.3508385967147 29.195061579133153 22.4604882071831 72-73 22.40394383953269 26.689125071171 28.251967239441143 22.654963849855168 74-75 22.29975857367355 27.10857464730377 27.65299839684016 22.93866838218252 76-77 21.655203840261184 27.192083362269038 28.213835297103255 22.93887750036652 78-79 22.184120883023137 27.22501362663616 27.61503062891311 22.97583486142759 80-81 22.08941962395991 27.358485521346392 27.60275351199405 22.94934134269965 82-83 22.29781367058522 27.27944150498826 27.45872492315432 22.964019901272202 84-85 22.135193699895474 27.202492390410903 27.352791266366953 23.309522643326673 86-87 22.25516856965511 27.11633404473488 27.503070405652885 23.12542697995713 88-89 22.01999772422579 27.149537187947292 27.368794344444414 23.461670743382502 90-91 21.997829367029908 27.297164102675985 27.452860174703016 23.25214635559109 92-93 22.17770544246687 27.09682980684433 27.42103393245252 23.304430818236284 94-95 21.87867656502864 26.898520383261836 27.598405644463853 23.62439740724567 96-97 21.99967861283995 26.866896715966714 27.486668959028698 23.646755712164634 98-99 21.82242402876442 26.921549869680472 27.22319721802465 24.032828883530453 100-101 21.68366091815592 26.74516409332071 27.621259720907226 23.94991526761614 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2032.0 1 1436.0 2 838.0 3 864.5 4 852.0 5 861.5 6 1300.5 7 2557.5 8 3863.5 9 4557.0 10 4363.0 11 3789.0 12 3684.0 13 3702.0 14 3920.0 15 4172.0 16 4336.5 17 4581.5 18 4758.5 19 5028.5 20 5591.0 21 6249.5 22 7144.5 23 8289.5 24 9643.5 25 11474.0 26 13803.5 27 16430.5 28 19043.5 29 21968.0 30 25254.5 31 29068.5 32 33172.0 33 36827.0 34 40922.5 35 44779.0 36 48976.5 37 53614.0 38 56806.0 39 58706.0 40 60800.0 41 64935.0 42 70159.0 43 74285.5 44 77333.0 45 80282.0 46 81963.5 47 84590.0 48 90948.0 49 93824.5 50 92177.0 51 90647.0 52 88443.5 53 87300.5 54 85068.5 55 78544.5 56 71085.5 57 63975.0 58 56997.0 59 50370.5 60 42371.5 61 33797.5 62 26612.5 63 20632.5 64 15735.5 65 11982.0 66 9064.0 67 6870.0 68 4897.5 69 3379.5 70 2476.5 71 1914.5 72 1594.0 73 1311.0 74 1020.5 75 772.0 76 533.0 77 305.5 78 164.5 79 103.0 80 68.5 81 38.0 82 21.0 83 12.0 84 8.5 85 6.5 86 3.0 87 3.0 88 2.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020019882994906053 2 0.01792378413432828 3 3.8499774990203943E-4 4 0.0794806465908877 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.00947522240036686 12-13 0.0 14-15 0.0 16-17 7.05829208153739E-4 18-19 8.555505553378654E-5 20-21 0.0 22-23 2.780539304848063E-4 24-25 0.002459707846596363 26-27 0.004042476373971414 28-29 0.0013261033607736914 30-31 2.35276402717913E-4 32-33 7.272179720371856E-4 34-35 0.0029088718881487424 36-37 0.08754421057494709 38-39 0.09569332961454026 40-41 0.0810848038821462 42-43 0.08459256115903145 44-45 0.08371562183981014 46-47 0.08198313196525096 48-49 0.07663594099438929 50-51 0.07622955448060381 52-53 0.0591613209016134 54-55 0.05019942883444926 56-57 0.02831872338168335 58-59 0.022308480730434843 60-61 0.01341075495492104 62-63 0.002117487624461217 64-65 0.006331074109500205 66-67 0.16937762119301392 68-69 0.7664663537633103 70-71 0.9305609502771128 72-73 0.3755011387377891 74-75 0.05785660630472315 76-77 0.21037988155758114 78-79 0.014629914496277499 80-81 0.0034008134574680152 82-83 0.0015613797634916045 84-85 2.1388763883446636E-5 86-87 0.0015827685273750512 88-89 4.277752776689327E-4 90-91 0.004662750526591367 92-93 0.00451302917940724 94-95 0.08281729375670538 96-97 0.03980448958709419 98-99 0.014715469551811286 100-101 0.013560476302105167 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2337676.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.19746899315448 #Duplication Level Percentage of deduplicated Percentage of total 1 73.13247937685743 36.7106536591231 2 13.978347271440466 14.033553074673568 3 5.339087525195942 8.040260414932836 4 2.6283699009445356 5.277500664208154 5 1.3808320802059217 3.465713776544488 6 0.8348520989202756 2.514447740965228 7 0.5563435779202511 1.9548927651534702 8 0.3644224178610246 1.4634466416791334 9 0.2704846083356187 1.2219878466047478 >10 1.339793248827277 12.133638944185519 >50 0.10165745423486529 3.5562765160982113 >100 0.06806543164979297 6.2481813370619275 >500 0.0041429568052288104 1.3725752420849482 >1k 8.631160010892429E-4 0.7177424703401939 >5k 8.631160010892429E-5 0.32425357044706093 >10k+ 1.7262320021784857E-4 0.9648753358973873 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11730 0.5017804007056581 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10540 0.4508751426630551 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7484 0.3201470178074292 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2725 0.11656876316478418 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2472 0.10574604863976017 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.1388763883446636E-5 0.0 12-13 0.0 0.0 0.0 4.277752776689327E-5 0.0 14-15 0.0 0.0 0.0 6.416629165033991E-5 0.0 16-17 0.0 0.0 0.0 1.0694381941723319E-4 0.0 18-19 0.0 0.0 0.0 1.2833258330067983E-4 0.0 20-21 0.0 0.0 0.0 1.711101110675731E-4 0.0 22-23 0.0 0.0 0.0 3.2083145825169954E-4 0.0 24-25 0.0 0.0 0.0 7.05829208153739E-4 0.0 26-27 0.0 0.0 0.0 0.0010694381941723319 0.0 28-29 0.0 0.0 0.0 0.003229703346400442 0.0 30-31 0.0 0.0 0.0 0.008277451622893848 0.0 32-33 0.0 0.0 0.0 0.01741045380112556 0.0 34-35 0.0 2.1388763883446636E-5 0.0 0.03122759526983209 0.0 36-37 0.0 4.277752776689327E-5 0.0 0.053407743416966254 0.0 38-39 0.0 4.277752776689327E-5 0.0 0.08656032743630854 0.0 40-41 0.0 4.277752776689327E-5 0.0 0.12178762155234515 0.0 42-43 0.0 4.277752776689327E-5 0.0 0.16047989541750013 0.0 44-45 0.0 4.277752776689327E-5 0.0 0.20819822764146956 0.0 46-47 4.277752776689327E-5 4.277752776689327E-5 0.0 0.2648570631687197 0.0 48-49 4.277752776689327E-5 4.277752776689327E-5 0.0 0.32523754361168955 0.0 50-51 4.277752776689327E-5 4.277752776689327E-5 0.0 0.3907941049144535 0.0 52-53 4.277752776689327E-5 4.277752776689327E-5 0.0 0.4552598392591617 0.0 54-55 4.277752776689327E-5 4.277752776689327E-5 0.0 0.5282169128655982 0.0 56-57 4.277752776689327E-5 4.277752776689327E-5 0.0 0.6131303054828813 0.0 58-59 4.277752776689327E-5 4.277752776689327E-5 0.0 0.6985570284333672 0.0 60-61 4.277752776689327E-5 4.277752776689327E-5 0.0 0.7851173558696757 0.0 62-63 4.277752776689327E-5 4.277752776689327E-5 0.0 0.8676993732236631 0.0 64-65 4.277752776689327E-5 4.277752776689327E-5 0.0 0.9659379657403335 4.277752776689327E-5 66-67 4.277752776689327E-5 4.277752776689327E-5 0.0 1.0698659694500008 4.277752776689327E-5 68-69 4.277752776689327E-5 4.277752776689327E-5 0.0 1.1883169438365282 4.277752776689327E-5 70-71 4.277752776689327E-5 4.277752776689327E-5 0.0 1.335557194410175 4.277752776689327E-5 72-73 4.277752776689327E-5 4.277752776689327E-5 0.0 1.5005928965348492 4.277752776689327E-5 74-75 4.277752776689327E-5 4.277752776689327E-5 0.0 1.6785046345173582 4.277752776689327E-5 76-77 4.277752776689327E-5 4.277752776689327E-5 0.0 1.8853339812702874 4.277752776689327E-5 78-79 4.277752776689327E-5 4.277752776689327E-5 0.0 2.1215514895990717 4.277752776689327E-5 80-81 4.277752776689327E-5 4.277752776689327E-5 0.0 2.3768049977841237 4.277752776689327E-5 82-83 4.277752776689327E-5 4.277752776689327E-5 0.0 2.640485678939254 4.277752776689327E-5 84-85 4.277752776689327E-5 4.277752776689327E-5 0.0 2.909427996009712 4.277752776689327E-5 86-87 4.277752776689327E-5 4.277752776689327E-5 0.0 3.1909255174797533 4.277752776689327E-5 88-89 4.277752776689327E-5 4.277752776689327E-5 0.0 3.5035436904002095 4.277752776689327E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8165 0.0 41.214462 1 GTATCAA 12510 0.0 32.36319 1 TATCAAC 16050 0.0 25.160067 2 ATCAACG 16355 0.0 24.656572 3 TCAACGC 16405 0.0 24.528833 4 CAACGCA 16550 0.0 24.205626 5 AACGCAG 17050 0.0 23.523588 6 GTACATG 11550 0.0 21.90306 1 TACATGG 11760 0.0 21.305117 2 GAGTACT 10455 0.0 21.285582 12-13 ACGCAGA 19565 0.0 20.499727 7 CGCAGAG 19700 0.0 20.335182 8 AGTACTT 10875 0.0 20.18021 12-13 GTACTTT 11160 0.0 19.962166 14-15 ACATGGG 11875 0.0 19.855785 3 AGAGTAC 17310 0.0 19.45731 10-11 GCAGAGT 20355 0.0 19.121838 9 GTACGAC 200 1.2992436E-5 18.976858 3 TACTTTT 12390 0.0 17.617014 14-15 ACTTTTT 12985 0.0 17.613026 16-17 >>END_MODULE