FastQCFastQC Report
Fri 27 May 2016
SRR939395_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939395_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3821137
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133300.34884904676278294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103060.2697102982698605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67900.17769580101420074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128050.038.970581
GTATCAA206000.036.177721
TATCAAC253800.029.042532
ATCAACG260900.028.3365943
TCAACGC264350.028.0538674
CAACGCA267050.027.5675625
AACGCAG272500.026.9957566
GTACATG185450.025.1742271
TACATGG182700.024.8708112
ACGCAGA300350.024.1022327
CGCAGAG300300.023.818288
ACATGGG187400.022.8464743
GAGTACT154200.022.75425112-13
GCAGAGT302350.022.025439
GTACTTT169500.020.93982514-15
AGTACTT160250.020.71369612-13
AACGCCG4750.020.4719626
AGAGTAC262800.019.9507610-11
CATGGGA126900.019.8575294
CATGGGG66150.019.4091284
Produced by FastQC (version 0.11.5)