FastQCFastQC Report
Fri 27 May 2016
SRR939395_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939395_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3821137
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194160.5081210121490017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172010.4501539724956211No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116200.3040979687459518No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46690.12218876214069269No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39120.1023779047963996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147200.036.9967651
GTATCAA226750.031.9603651
TATCAAC286100.025.1584242
ATCAACG285600.025.0654743
TCAACGC289000.024.7705844
CAACGCA293250.024.3954355
AACGCAG301000.023.7830546
GTACATG204950.022.8255231
TACATGG204300.022.4285722
GAGTACT175200.021.70104212-13
ACGCAGA338100.021.1733197
CGCAGAG340500.020.9127678
ACATGGG207600.020.9044043
AGTACTT182850.020.30082512-13
GTACTTT189300.020.21004314-15
GCAGAGT353950.019.4622089
AGAGTAC304400.019.15166910-11
CATGGGA142550.018.1798274
ACTTTTT221450.017.82151616-17
TACTTTT211700.017.42260614-15
Produced by FastQC (version 0.11.5)