##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939395_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3821137 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.99570232629712 31.0 31.0 31.0 30.0 34.0 2 31.24917295558887 31.0 31.0 34.0 30.0 34.0 3 31.236643962255215 31.0 31.0 34.0 28.0 34.0 4 35.17420652543994 35.0 35.0 37.0 33.0 37.0 5 34.79244083632699 35.0 35.0 37.0 32.0 37.0 6 34.74785515410727 35.0 35.0 37.0 32.0 37.0 7 34.619892194391355 35.0 35.0 37.0 30.0 37.0 8 34.63870753652643 35.0 35.0 37.0 30.0 37.0 9 35.9893173157623 37.0 35.0 39.0 30.0 39.0 10-11 35.846709631190926 37.0 35.0 39.0 30.0 39.0 12-13 35.80993549825615 37.0 35.0 39.0 30.0 39.0 14-15 36.66740750200791 38.0 35.5 40.0 31.0 41.0 16-17 36.67759569991864 38.0 35.5 40.0 31.0 41.0 18-19 36.652354521703884 38.0 35.0 40.0 30.0 41.0 20-21 36.56091616186491 38.0 35.0 40.0 30.0 41.0 22-23 36.4272142035211 38.0 35.0 40.0 30.0 41.0 24-25 36.20666244104831 38.0 34.0 40.0 29.5 41.0 26-27 35.83541704995136 38.0 34.0 40.0 28.0 41.0 28-29 35.553153550893356 38.0 34.0 40.0 27.0 41.0 30-31 35.33751629423389 38.0 34.0 40.0 27.0 41.0 32-33 35.12505741092245 38.0 33.5 40.0 27.0 41.0 34-35 35.0693840602941 38.0 33.0 40.0 26.5 41.0 36-37 35.05666009881352 38.0 33.5 40.0 26.0 41.0 38-39 35.03212015167213 38.0 33.0 40.0 26.0 41.0 40-41 34.811158825239715 38.0 33.0 40.0 25.5 41.0 42-43 34.4967903532378 38.0 33.0 40.0 24.5 41.0 44-45 34.31039792606232 38.0 33.0 40.0 24.0 41.0 46-47 33.965987348791735 37.0 33.0 40.0 23.5 41.0 48-49 33.64723209348422 37.0 32.5 40.0 22.5 41.0 50-51 33.38624459159669 37.0 32.0 40.0 21.5 41.0 52-53 33.01293855729328 37.0 31.0 40.0 19.0 41.0 54-55 32.805495851104 37.0 31.0 39.5 18.5 41.0 56-57 32.42226894769803 36.0 31.0 39.0 17.5 40.0 58-59 32.00030841605522 36.0 30.0 39.0 14.0 40.0 60-61 31.727501395527035 35.0 30.0 39.0 13.0 40.0 62-63 31.353019925744615 35.0 29.0 39.0 11.0 40.0 64-65 30.934473561141616 35.0 29.0 38.0 8.0 40.0 66-67 29.288433521226796 33.5 25.5 37.5 5.0 39.5 68-69 25.694951528825058 30.5 16.5 36.0 2.0 39.0 70-71 25.09610725812762 29.5 15.0 35.0 2.0 38.0 72-73 26.883665778013196 31.0 22.0 35.0 2.0 38.0 74-75 27.473848752347795 32.0 24.5 35.0 2.0 38.0 76-77 27.4895389775347 31.5 25.5 34.5 2.0 37.0 78-79 28.306103916190388 33.0 26.0 35.0 2.0 37.0 80-81 28.14915495047678 33.0 26.0 35.0 2.0 37.0 82-83 27.73708506133122 33.0 26.0 35.0 2.0 36.0 84-85 27.394970920958865 32.5 26.0 35.0 2.0 36.0 86-87 26.94375496089253 32.0 25.5 35.0 2.0 35.5 88-89 26.45921187855866 32.0 25.0 34.0 2.0 35.0 90-91 26.017240418231538 32.0 24.0 34.0 2.0 35.0 92-93 25.468467238939613 31.5 21.5 34.0 2.0 35.0 94-95 24.12730948929599 31.0 15.0 34.0 2.0 35.0 96-97 23.333389250372335 30.5 4.5 34.0 2.0 35.0 98-99 23.002122404928166 31.0 2.0 34.0 2.0 35.0 100-101 21.7408875944516 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3795112328904402 1101 2 0.3026800870575599 1101 3 0.3340313877766796 1101 4 0.2300069934347846 1101 5 0.36707137238368404 1101 6 0.5117461383907909 1101 7 0.5857805323209959 1101 8 0.5197755776324939 1101 9 0.6256885822678342 1101 10-11 0.714682303679659 1101 12-13 0.7006234467553085 1101 14-15 0.7355013693287233 1101 16-17 0.7133733863275822 1101 18-19 0.7782580202820952 1101 20-21 0.6966160198965881 1101 22-23 0.8500290063801188 1101 24-25 0.7799051090580846 1101 26-27 0.8776195966165758 1101 28-29 0.7297678317030147 1101 30-31 0.8211052887060006 1101 32-33 0.6888330496844262 1101 34-35 0.7577705132102466 1101 36-37 0.825336133445866 1101 38-39 0.8982539339676308 1101 40-41 0.986515816905019 1101 42-43 0.8229209957998762 1101 44-45 0.5275855728742016 1101 46-47 0.5915341917262893 1101 48-49 0.5868179917367016 1101 50-51 0.547831519336718 1101 52-53 0.7448647422196544 1101 54-55 0.6015434931772674 1101 56-57 0.5913592532690402 1101 58-59 0.5010154168743242 1101 60-61 0.44977939345564266 1101 62-63 0.3735575694440385 1101 64-65 0.07793896210972662 1101 66-67 -2.9721103479796476 1101 68-69 -8.082629190234371 1101 70-71 -11.406549637875255 1101 72-73 -11.218067514341312 1101 74-75 -9.489053591141872 1101 76-77 -4.575946847457011 1101 78-79 -1.5516176071097867 1101 80-81 -0.9611027062608919 1101 82-83 -0.5846371821868068 1101 84-85 -0.5979695378803136 1101 86-87 -0.7054964061039044 1101 88-89 -0.5671589670478348 1101 90-91 -0.7126713367504003 1101 92-93 -1.2557424391148828 1101 94-95 -4.81343648561797 1101 96-97 -1.685926659987004 1101 98-99 -1.001285267291422 1101 100-101 -0.9663679361156916 1102 1 0.4268751664555772 1102 2 0.27709294435778276 1102 3 0.3802559187875616 1102 4 0.2895649863984673 1102 5 0.4151073219407877 1102 6 0.5118433498169281 1102 7 0.4213218885635328 1102 8 0.5408674698136977 1102 9 0.7160374542854413 1102 10-11 0.7331403698942083 1102 12-13 0.6763546017726796 1102 14-15 0.6447216117100396 1102 16-17 0.7985343919741936 1102 18-19 0.7028011981497571 1102 20-21 0.880901904884638 1102 22-23 0.8294362718451112 1102 24-25 0.801428135781272 1102 26-27 0.7817699395015651 1102 28-29 0.618388306968555 1102 30-31 0.6477400369987336 1102 32-33 0.6792955632283721 1102 34-35 0.7138149541418599 1102 36-37 0.9493043200420175 1102 38-39 1.0242991590631192 1102 40-41 1.0788796477820313 1102 42-43 1.1170313027626904 1102 44-45 1.0391038821671046 1102 46-47 1.1375689986446034 1102 48-49 1.0746037370066759 1102 50-51 1.0317254779764298 1102 52-53 1.082278196355709 1102 54-55 1.010530322335974 1102 56-57 0.9435494354128835 1102 58-59 0.8872585686654162 1102 60-61 0.9035874114690117 1102 62-63 0.7293254059047989 1102 64-65 0.7817463577708104 1102 66-67 -2.229811770739193 1102 68-69 -7.393523031249504 1102 70-71 -10.99580961122613 1102 72-73 -8.963474149985792 1102 74-75 -7.223695835045461 1102 76-77 -3.5199929587821437 1102 78-79 -0.7960417663274164 1102 80-81 -0.0608359038384485 1102 82-83 0.15806489715439653 1102 84-85 0.047363380136598465 1102 86-87 0.1681913485548705 1102 88-89 0.2429456690410028 1102 90-91 0.14973276654265177 1102 92-93 -0.12556846675852995 1102 94-95 -3.580878530617774 1102 96-97 -0.4341601441577829 1102 98-99 0.3195626939136247 1102 100-101 0.32385304250837166 1103 1 0.39150622116815015 1103 2 0.27994212781533534 1103 3 0.38787817809416225 1103 4 0.21977155098237233 1103 5 0.4042692670414425 1103 6 0.4271615437198264 1103 7 0.49973654964198744 1103 8 0.47758812243926485 1103 9 0.6558996796685577 1103 10-11 0.688338462732375 1103 12-13 0.5640240899210553 1103 14-15 0.7329423565057738 1103 16-17 0.749772361943144 1103 18-19 0.6583075591075698 1103 20-21 0.8213414561650083 1103 22-23 0.7138517578921295 1103 24-25 0.8951588309997973 1103 26-27 0.846770161561686 1103 28-29 0.9338717379373662 1103 30-31 1.0123788790251709 1103 32-33 0.9701561770025435 1103 34-35 1.0354329632785664 1103 36-37 0.925795058309788 1103 38-39 0.9020438373770929 1103 40-41 0.9778441479733644 1103 42-43 1.0072484288910104 1103 44-45 0.9838216194170499 1103 46-47 0.9352896579624002 1103 48-49 0.8206081149371798 1103 50-51 0.7868458497719928 1103 52-53 0.9547998076726216 1103 54-55 0.9106368503560063 1103 56-57 0.9028174948325187 1103 58-59 0.9654639456839647 1103 60-61 0.843156152461134 1103 62-63 0.7506308578153984 1103 64-65 0.6392241867407122 1103 66-67 -2.7073066466258418 1103 68-69 -7.493457508304001 1103 70-71 -11.283250407868337 1103 72-73 -8.988451837506439 1103 74-75 -7.25799858755672 1103 76-77 -3.4900715437298757 1103 78-79 -0.695226684003952 1103 80-81 -0.1270432812232336 1103 82-83 0.20662500457065747 1103 84-85 0.015002741114489737 1103 86-87 0.15051615376555816 1103 88-89 0.2610757524686882 1103 90-91 0.2284624183828008 1103 92-93 0.41139441391488063 1103 94-95 1.1048590953100899 1103 96-97 1.1811712516756359 1103 98-99 0.8140542340313708 1103 100-101 0.9096037182707839 1104 1 0.3944901504955993 1104 2 0.27995983040433003 1104 3 0.3770309613911671 1104 4 0.18807926751707527 1104 5 0.5195819647985829 1104 6 0.5370351955141928 1104 7 0.5929303094853253 1104 8 0.4496354959979314 1104 9 0.6416273398208148 1104 10-11 0.6471237659118643 1104 12-13 0.6316557880934681 1104 14-15 0.742113698314931 1104 16-17 0.7867201089668256 1104 18-19 0.7960901173128789 1104 20-21 0.8628209467619925 1104 22-23 0.7428858813907482 1104 24-25 0.8883366585130403 1104 26-27 1.0055850048189612 1104 28-29 0.8974652907855827 1104 30-31 0.9266317400645505 1104 32-33 0.7376075769882462 1104 34-35 0.8969500799237906 1104 36-37 0.8896059298094414 1104 38-39 0.9317628782162473 1104 40-41 1.0219902624011112 1104 42-43 1.072442981238133 1104 44-45 0.8284003088258416 1104 46-47 0.8860258859889143 1104 48-49 0.9369924451776157 1104 50-51 0.8766588323500883 1104 52-53 0.9116050136881455 1104 54-55 0.8815091177125467 1104 56-57 0.8394907146360353 1104 58-59 1.072382248593378 1104 60-61 0.9123767623039925 1104 62-63 0.9336272886277506 1104 64-65 0.6472457841377057 1104 66-67 -2.3788545985584797 1104 68-69 -7.205428279124195 1104 70-71 -8.62869267963622 1104 72-73 -8.123673212554145 1104 74-75 -6.868744464388307 1104 76-77 -3.279647633480689 1104 78-79 -0.35124424997902537 1104 80-81 0.42129891304068323 1104 82-83 0.609209147449743 1104 84-85 0.5960210238358066 1104 86-87 0.7510928170921787 1104 88-89 0.8060135064106895 1104 90-91 0.7245938364430309 1104 92-93 0.8541033224954333 1104 94-95 1.6992629493008025 1104 96-97 1.7294329498236074 1104 98-99 1.3338908790700863 1104 100-101 1.2729517713764054 1105 1 0.5545608729766016 1105 2 0.4438141633937249 1105 3 0.5395281654392932 1105 4 0.3761568361068939 1105 5 0.47894855898260147 1105 6 0.6079200088654204 1105 7 0.6497758377041194 1105 8 0.5477784209524117 1105 9 0.8205627469108165 1105 10-11 0.8891644958491227 1105 12-13 0.8553623751860044 1105 14-15 1.0420491641683114 1105 16-17 1.061133475159174 1105 18-19 1.1005483688691413 1105 20-21 1.0772639163226785 1105 22-23 1.2059882308817436 1105 24-25 1.2096431958267857 1105 26-27 1.2881026435072656 1105 28-29 1.2463327880605917 1105 30-31 1.1369197521371959 1105 32-33 1.230959310724721 1105 34-35 1.2998072465744812 1105 36-37 1.3660127290483004 1105 38-39 1.1567492987163774 1105 40-41 1.188288494843448 1105 42-43 1.3796333190853076 1105 44-45 1.1430407514676872 1105 46-47 1.1736939407301037 1105 48-49 1.1026855966877633 1105 50-51 1.1201990252651939 1105 52-53 1.302349477586148 1105 54-55 1.2971677853275523 1105 56-57 1.3307926212552843 1105 58-59 1.4459883045752804 1105 60-61 1.4440948203352306 1105 62-63 1.5470665187970702 1105 64-65 1.5161736716273104 1105 66-67 2.127557587909312 1105 68-69 -4.739198568761939 1105 70-71 -5.072757543842979 1105 72-73 -1.2661405516724074 1105 74-75 1.5667514744292887 1105 76-77 1.499036340269793 1105 78-79 1.4264269059668848 1105 80-81 1.351596170081411 1105 82-83 1.568500821266344 1105 84-85 1.5514795898523488 1105 86-87 1.7531830220355964 1105 88-89 1.6864508044262578 1105 90-91 1.708174576085046 1105 92-93 1.883569693601494 1105 94-95 2.6725313915283735 1105 96-97 2.8307410330480245 1105 98-99 2.572722700761606 1105 100-101 2.459019626767841 1106 1 0.18025355974525326 1106 2 0.21130059671695633 1106 3 0.17112811643264791 1106 4 0.055167960546455674 1106 5 0.35109460797110614 1106 6 0.30548195123255084 1106 7 0.2949909209574315 1106 8 0.3359803811493265 1106 9 0.4911132782390766 1106 10-11 0.4796851834164926 1106 12-13 0.46169783899176053 1106 14-15 0.4737084903636841 1106 16-17 0.3655491082330471 1106 18-19 0.5152234133603528 1106 20-21 0.538570368263386 1106 22-23 0.5051454382975464 1106 24-25 0.520954034022985 1106 26-27 0.7310725380637493 1106 28-29 0.8639104141617011 1106 30-31 0.659112884478219 1106 32-33 0.5663196318537445 1106 34-35 0.6866908843832178 1106 36-37 0.6910431027193837 1106 38-39 0.6221473692002917 1106 40-41 0.7216221994392455 1106 42-43 0.7519272606674932 1106 44-45 0.632926011846493 1106 46-47 0.6174312003515325 1106 48-49 0.5452510543745817 1106 50-51 0.6168477595721242 1106 52-53 0.8716576537156655 1106 54-55 0.8383664364276839 1106 56-57 0.7389059079006373 1106 58-59 1.0452469986905726 1106 60-61 0.9267391709127821 1106 62-63 0.87056388468989 1106 64-65 0.8686646456435696 1106 66-67 1.442217108271123 1106 68-69 -5.145099781791231 1106 70-71 -5.8722479401825005 1106 72-73 -2.013014971455199 1106 74-75 0.715301087858947 1106 76-77 0.8522414873913462 1106 78-79 0.7166983577703725 1106 80-81 0.7363507516228758 1106 82-83 0.8325236915699357 1106 84-85 0.8398515748614592 1106 86-87 0.8242328401380377 1106 88-89 0.7427277254999503 1106 90-91 0.7387628521752596 1106 92-93 0.8034210362836731 1106 94-95 1.7385437042757417 1106 96-97 1.7841418048682414 1106 98-99 1.5643255476021736 1106 100-101 1.5268382844978738 1107 1 0.02433355782858726 1107 2 0.04339354018812003 1107 3 0.008685995669090119 1107 4 -0.07713214416827441 1107 5 0.18164023777151073 1107 6 0.1288711175307995 1107 7 0.20413426097956489 1107 8 0.14158045814084375 1107 9 0.18698547835575852 1107 10-11 0.12995064229712483 1107 12-13 0.23288992487651683 1107 14-15 0.24006018136642382 1107 16-17 0.09966115304870726 1107 18-19 0.38222799497046367 1107 20-21 0.26419806056625106 1107 22-23 0.28252828713794287 1107 24-25 0.49610295128918835 1107 26-27 0.39306736898146255 1107 28-29 0.35190383902127564 1107 30-31 0.3035144984923477 1107 32-33 0.31063293650630897 1107 34-35 0.37747559395634056 1107 36-37 0.36697585186005455 1107 38-39 0.36188857746472536 1107 40-41 0.35505487186105 1107 42-43 0.49418386157908145 1107 44-45 0.3788149972970558 1107 46-47 0.34313503141617474 1107 48-49 0.41266626320391 1107 50-51 0.3690873848754421 1107 52-53 0.5887278132787159 1107 54-55 0.391446755483031 1107 56-57 0.3629887403762737 1107 58-59 0.3825227729376621 1107 60-61 0.46278268063299777 1107 62-63 0.48031371853336324 1107 64-65 0.37065043156643185 1107 66-67 1.039984905409387 1107 68-69 -5.319946603654895 1107 70-71 -5.945370011704441 1107 72-73 -2.3808010123109433 1107 74-75 0.11918586441677093 1107 76-77 0.10341371976766212 1107 78-79 0.23257336211391078 1107 80-81 0.10859620349932086 1107 82-83 0.29815845419074094 1107 84-85 0.32401769995190577 1107 86-87 0.34039218287460216 1107 88-89 0.3544007809580343 1107 90-91 0.39735313473446965 1107 92-93 0.5543654904899924 1107 94-95 1.3060281718233107 1107 96-97 1.5648383576473677 1107 98-99 1.105525116259411 1107 100-101 1.0380940589340817 1108 1 0.07559980771927854 1108 2 -0.029267127280419913 1108 3 0.04258987980338702 1108 4 -0.020867387477338184 1108 5 0.033092848891143944 1108 6 0.21189921113545296 1108 7 0.17751776912452044 1108 8 0.07169128491152321 1108 9 0.11869056397962652 1108 10-11 0.2609923264944527 1108 12-13 0.2979534229898064 1108 14-15 0.29871612009408466 1108 16-17 0.014881643379204945 1108 18-19 0.21744473992698943 1108 20-21 0.30590638833103156 1108 22-23 0.27411563272376327 1108 24-25 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-3.915871443579082 2315 92-93 -4.802516001592835 2315 94-95 -2.9535181329931497 2315 96-97 -1.5400600617574582 2315 98-99 -2.917861965085912 2315 100-101 -2.2581251272979372 2316 1 -0.3557630265814673 2316 2 -0.34958111821991267 2316 3 -0.941741768263693 2316 4 -0.3258392899197986 2316 5 -0.29408551921915205 2316 6 -2.388777831369076 2316 7 -0.6045290806900709 2316 8 -0.4345502924272253 2316 9 -0.12107746235045624 2316 10-11 -0.09352427513973538 2316 12-13 -0.6322372117448865 2316 14-15 -1.1301885449225466 2316 16-17 -1.3850108988772334 2316 18-19 -0.20415092682481628 2316 20-21 -4.166993744963548 2316 22-23 -4.493883355822884 2316 24-25 -3.0062443371150422 2316 26-27 -2.3129855245573268 2316 28-29 -1.5040886388843333 2316 30-31 -0.3042041207338215 2316 32-33 -2.049221983539155 2316 34-35 -2.6058655462687526 2316 36-37 -4.921616282530103 2316 38-39 -2.360028823849696 2316 40-41 -1.5958745543259951 2316 42-43 -3.077266280318881 2316 44-45 -0.759557319812842 2316 46-47 -0.8191938337005169 2316 48-49 -0.31874841885744587 2316 50-51 -0.10450710898516746 2316 52-53 -2.5895996995389936 2316 54-55 -1.0411471305574764 2316 56-57 0.025448539616114374 2316 58-59 0.21692458489373223 2316 60-61 -1.2363531449948155 2316 62-63 -0.8760451367717472 2316 64-65 0.16734665084477385 2316 66-67 1.2463056208272043 2316 68-69 3.7546023124908423 2316 70-71 3.9980649357900155 2316 72-73 2.234166566952979 2316 74-75 1.1161863326470893 2316 76-77 -0.1399262260671037 2316 78-79 0.27195412385648865 2316 80-81 -0.21644810068771037 2316 82-83 0.14587332171234024 2316 84-85 0.3634709586224112 2316 86-87 0.07659688305698609 2316 88-89 0.30373431299004494 2316 90-91 0.4187766409954925 2316 92-93 0.5871858069228253 2316 94-95 1.2112984099612945 2316 96-97 1.2769022677881807 2316 98-99 0.17396134607476554 2316 100-101 -0.14012711284342672 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 35.0 4 116.0 5 291.0 6 652.0 7 1714.0 8 3633.0 9 6145.0 10 10006.0 11 14846.0 12 19414.0 13 24779.0 14 30455.0 15 38243.0 16 45260.0 17 51411.0 18 46805.0 19 39384.0 20 37439.0 21 38328.0 22 40883.0 23 46301.0 24 56272.0 25 70620.0 26 87825.0 27 109492.0 28 133410.0 29 161139.0 30 188994.0 31 219578.0 32 254640.0 33 295966.0 34 344888.0 35 399045.0 36 436895.0 37 385406.0 38 170398.0 39 10422.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.36485558641165 20.61094019411854 15.685886997240752 22.33831722222906 2 15.598754260699584 21.59493474736296 39.33792484625883 23.468386145678625 3 19.124098403171622 25.54972512108307 30.719128887553627 24.607047588191683 4 12.968496026182782 18.020395500082827 37.30544076278866 31.705667710945722 5 13.017880317571063 38.4990666371117 34.20496926431952 14.278083780997711 6 30.73514506284386 37.409127178638194 17.41722424503492 14.438503513483028 7 27.42414103446173 33.924405222843355 20.653459951841562 17.99799379085335 8 26.141093606431802 35.39914952015591 19.549207474110457 18.910549399301832 9 26.039683999814716 17.059320301784524 20.643175054964008 36.25782064343676 10-11 24.46222158483195 27.670128027338457 27.07793518002626 20.789715207803333 12-13 25.6196912070936 25.033923149052235 27.866234578870113 21.48015106498406 14-15 22.52682905637772 26.129801156043346 26.940894817432614 24.402474970146322 16-17 21.248570119307857 29.638001707874885 27.403161321946456 21.710266850870806 18-19 21.013503204449616 27.67783982227075 30.493827289730973 20.81482968354866 20-21 22.17965632981514 26.58603092877259 30.59931546507981 20.634997276332463 22-23 21.68804730110436 26.781806043593832 30.359981858802758 21.17016479649905 24-25 21.458822334818144 26.871661497611836 30.626852688087343 21.04266347948268 26-27 21.39353681473935 27.07029014275661 30.455097054030507 21.081075988473533 28-29 21.03265167127044 27.209438132015435 30.659833730192283 21.09807646652185 30-31 21.329780823434316 26.94241408671982 30.551375760100115 21.176429329745755 32-33 21.210230058005887 27.007625851944304 30.39450442431322 21.38763966573659 34-35 21.737480992150836 27.037445135691833 30.172922353754068 21.05215151840326 36-37 21.550247737797047 26.80470963128741 30.301265979634774 21.343776651280773 38-39 21.309623110891284 27.112679008179935 30.41018363467556 21.16751424625322 40-41 21.610260697932564 26.96848601358795 30.320786334545907 21.100466953933577 42-43 21.4329510818829 27.04951541910232 30.01940314597393 21.49813035304085 44-45 21.6099661389555 27.28486081764232 29.73253023157853 21.37264281182365 46-47 21.677765874412497 27.399471539481485 29.264578540751618 21.658184045354396 48-49 21.719128957266783 27.585220440645518 29.01907881603828 21.67657178604942 50-51 21.68450798016038 27.729313609673618 28.876574226801825 21.709604183364178 52-53 22.016671346048682 27.741288492282163 28.63422981911744 21.607810342551716 54-55 21.67465754366329 27.614347099499547 28.835663512957737 21.87533184387943 56-57 21.864496901070396 27.95069829981679 28.53566478676631 21.6491400123465 58-59 21.92732052681049 27.587703811620518 28.624753664506304 21.86022199706269 60-61 21.87699075530532 27.586653636188995 28.88324548927891 21.653110119226778 62-63 21.878814787015727 27.55498887287679 28.860618436963374 21.705577903144107 64-65 21.673358062775723 27.608293379280003 28.75446076716755 21.96388779077672 66-67 21.285695028060427 27.65344286496229 29.128136186824623 21.932725920152663 68-69 21.4393432607785 27.237658310322526 29.55681287015573 21.766185558743246 70-71 20.946369599715723 27.35726469461563 29.781249595745113 21.915116109923538 72-73 21.678114957706576 27.478548887124294 28.718722102007003 22.124614053162126 74-75 21.327154075360582 27.747772113851088 28.716842147057587 22.208231663730746 76-77 21.513595304150655 27.753064304224072 28.45454422912001 22.278796162505262 78-79 21.50864834009502 27.829446834438208 28.305500091473206 22.356404733993564 80-81 21.323879444334093 27.905460063181202 28.394262553999884 22.376397938484818 82-83 21.511673096253812 27.898319269892706 28.19336234215104 22.396645291702445 84-85 21.383598832441606 27.793838299155237 28.13876185789228 22.68380101051088 86-87 21.404159218492243 27.734151172435666 28.249849880656917 22.611839728415166 88-89 21.414560907464537 27.675035617941184 28.155197393007477 22.755206081586806 90-91 21.219852803008216 27.82013723101951 28.29248715389314 22.667522812079135 92-93 21.36022947508007 27.72956046194932 28.2313703614578 22.67883970151281 94-95 20.838548119582175 27.538149680188916 28.543217770522826 23.08008442970608 96-97 20.717174525406925 27.41759207289246 28.641305203082297 23.223928198618317 98-99 20.925112784159023 27.388608302631123 28.150182747504825 23.536096165705032 100-101 20.788551385052827 27.31276380073539 28.412381187433404 23.486303626778376 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2646.0 1 1796.0 2 982.5 3 1001.5 4 973.5 5 1062.5 6 1606.0 7 2986.5 8 4607.5 9 5569.0 10 5587.5 11 5185.5 12 5102.5 13 5342.0 14 5758.5 15 6175.0 16 6490.0 17 6874.0 18 7342.0 19 8007.5 20 8940.0 21 10060.0 22 11624.5 23 13979.0 24 16823.0 25 20370.5 26 25537.5 27 31419.5 28 36968.0 29 43178.0 30 49822.0 31 57519.5 32 65886.0 33 74294.0 34 83197.5 35 91559.5 36 101410.0 37 111089.5 38 115957.5 39 118315.5 40 121187.0 41 125645.0 42 130752.5 43 135043.5 44 138619.0 45 141648.0 46 143362.5 47 147125.0 48 154975.0 49 155008.5 50 146560.5 51 137375.0 52 127669.5 53 120963.5 54 114499.5 55 104099.0 56 92241.5 57 80195.0 58 69926.5 59 60328.5 60 49545.5 61 38810.0 62 29839.0 63 22542.5 64 16546.5 65 12329.0 66 9135.0 67 6678.0 68 4591.0 69 3131.0 70 2295.0 71 1690.5 72 1325.5 73 1075.5 74 834.5 75 654.0 76 444.5 77 271.5 78 178.5 79 112.0 80 68.0 81 37.5 82 21.0 83 15.5 84 8.5 85 4.5 86 3.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023396177629852057 2 0.019758516902168125 3 0.0 4 0.0 5 2.093617684997947E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.663830948746407E-4 18-19 0.0011514897267488708 20-21 6.542555265618583E-5 22-23 0.0 24-25 0.0 26-27 4.7106397912453804E-4 28-29 0.018842559164981523 30-31 0.004619044017526721 32-33 0.00832213029786684 34-35 0.009081066708678595 36-37 0.10194609614886878 38-39 0.10449769270246002 40-41 0.12021291045047587 42-43 0.1039612031706793 44-45 0.09868790362659073 46-47 0.10003567001130814 48-49 0.10256109634383692 50-51 0.10014035089555805 52-53 0.07769938633448631 54-55 0.0657265101984043 56-57 0.03744958634040078 58-59 0.01664426059573368 60-61 0.009185747592928492 62-63 0.001792660142779492 64-65 0.00485457600708899 66-67 0.40825544857459967 68-69 0.7894247183495384 70-71 0.8715730422646454 72-73 0.27162072440742113 74-75 0.14311185388014092 76-77 0.014956281337204084 78-79 0.009054896487616121 80-81 0.0016748941479983576 82-83 0.0 84-85 1.3085110531237169E-5 86-87 2.747873211559805E-4 88-89 7.589364108117558E-4 90-91 0.003532979843434035 92-93 0.001792660142779492 94-95 0.21619219619710048 96-97 0.219751346261597 98-99 0.014786174900298 100-101 0.008060428087242095 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3821137.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.50151410853312 #Duplication Level Percentage of deduplicated Percentage of total 1 68.35635089084423 31.10317464467589 2 15.111951262729189 13.752333271770745 3 6.384640264060823 8.715323971592166 4 3.252217629700855 5.9192330544741445 5 1.966659798088042 4.4742999274693975 6 1.215880618807646 3.3194645478580855 7 0.7890438430854256 2.513188269088188 8 0.557290628870586 2.0286053929686614 9 0.42189852699714087 1.7277319600676773 >10 1.7669157658713845 13.676305164920299 >50 0.10399729618926273 3.2662467276367053 >100 0.06693367400991655 5.745451136483358 >500 0.004650318335635994 1.4420038646508762 >1k 0.001395095475413881 1.0407903902477393 >5k 0.0 0.0 >10k+ 1.7438693442673494E-4 1.2758476760960653 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19416 0.5081210121490017 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17201 0.4501539724956211 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11620 0.3040979687459518 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4669 0.12218876214069269 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3912 0.1023779047963996 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.3085110531237169E-5 0.0 14-15 0.0 0.0 0.0 2.6170221062474338E-5 0.0 16-17 0.0 0.0 0.0 3.92553315937115E-5 0.0 18-19 0.0 0.0 0.0 7.8510663187423E-5 0.0 20-21 0.0 0.0 0.0 1.308511053123717E-4 0.0 22-23 0.0 0.0 0.0 4.841490896557752E-4 0.0 24-25 0.0 0.0 0.0 9.159577371866017E-4 0.0 26-27 0.0 0.0 0.0 0.0015309579321547487 0.0 28-29 0.0 0.0 0.0 0.0031535116380281576 0.0 30-31 0.0 0.0 0.0 0.007890321650336013 0.0 32-33 0.0 0.0 0.0 0.015610536863765942 0.0 34-35 0.0 0.0 0.0 0.02726937034709826 0.0 36-37 0.0 0.0 0.0 0.04819246208654649 0.0 38-39 0.0 0.0 0.0 0.07819662053467333 0.0 40-41 0.0 0.0 0.0 0.1123749292422648 0.0 42-43 0.0 1.3085110531237169E-5 0.0 0.14699813170791837 0.0 44-45 0.0 2.6170221062474338E-5 0.0 0.19089867754021905 0.0 46-47 0.0 2.6170221062474338E-5 0.0 0.24340922610207383 0.0 48-49 0.0 2.6170221062474338E-5 0.0 0.30169030840820416 0.0 50-51 0.0 2.6170221062474338E-5 0.0 0.366069052221891 0.0 52-53 0.0 2.6170221062474338E-5 0.0 0.42843268901376735 0.0 54-55 0.0 2.6170221062474338E-5 0.0 0.49839877502429253 0.0 56-57 0.0 2.6170221062474338E-5 0.0 0.5791077367809634 0.0 58-59 0.0 2.6170221062474338E-5 0.0 0.6631272315020372 0.0 60-61 0.0 2.6170221062474338E-5 0.0 0.7486122586026096 0.0 62-63 0.0 2.6170221062474338E-5 0.0 0.8345814347928379 1.3085110531237169E-5 64-65 0.0 3.92553315937115E-5 0.0 0.9252089103321864 2.6170221062474338E-5 66-67 0.0 5.2340442124948675E-5 0.0 1.0218817069369666 2.6170221062474338E-5 68-69 0.0 5.2340442124948675E-5 0.0 1.141793659845224 2.6170221062474338E-5 70-71 0.0 5.2340442124948675E-5 0.0 1.2988280713306013 2.6170221062474338E-5 72-73 0.0 5.2340442124948675E-5 0.0 1.4678876993941854 2.6170221062474338E-5 74-75 0.0 5.2340442124948675E-5 0.0 1.651079246831506 2.6170221062474338E-5 76-77 0.0 5.2340442124948675E-5 0.0 1.8564500566192732 2.6170221062474338E-5 78-79 0.0 5.2340442124948675E-5 0.0 2.0943635362982276 2.6170221062474338E-5 80-81 0.0 5.2340442124948675E-5 0.0 2.353487980148317 2.6170221062474338E-5 82-83 0.0 5.2340442124948675E-5 0.0 2.6255535983138003 2.6170221062474338E-5 84-85 0.0 5.2340442124948675E-5 0.0 2.898888472200814 2.6170221062474338E-5 86-87 0.0 5.2340442124948675E-5 0.0 3.179825795306476 2.6170221062474338E-5 88-89 0.0 5.2340442124948675E-5 0.0 3.4874567438958612 2.6170221062474338E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14720 0.0 36.996765 1 GTATCAA 22675 0.0 31.960365 1 TATCAAC 28610 0.0 25.158424 2 ATCAACG 28560 0.0 25.065474 3 TCAACGC 28900 0.0 24.770584 4 CAACGCA 29325 0.0 24.395435 5 AACGCAG 30100 0.0 23.783054 6 GTACATG 20495 0.0 22.825523 1 TACATGG 20430 0.0 22.428572 2 GAGTACT 17520 0.0 21.701042 12-13 ACGCAGA 33810 0.0 21.173319 7 CGCAGAG 34050 0.0 20.912767 8 ACATGGG 20760 0.0 20.904404 3 AGTACTT 18285 0.0 20.300825 12-13 GTACTTT 18930 0.0 20.210043 14-15 GCAGAGT 35395 0.0 19.462208 9 AGAGTAC 30440 0.0 19.151669 10-11 CATGGGA 14255 0.0 18.179827 4 ACTTTTT 22145 0.0 17.821516 16-17 TACTTTT 21170 0.0 17.422606 14-15 >>END_MODULE