FastQCFastQC Report
Fri 27 May 2016
SRR939394_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939394_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3792942
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130910.34514105409468426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95970.2530225877432347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67570.17814667347932028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120300.038.9947971
GTATCAA198300.037.6873441
TATCAAC243800.030.1163482
ATCAACG253000.029.1120193
TCAACGC257550.028.7711184
CAACGCA257900.028.3923655
AACGCAG265550.027.5508656
ACGCAGA294000.024.3563947
CGCAGAG297250.024.0414228
GTACATG178100.022.9939441
GCAGAGT297250.022.5385279
GAGTACT152400.022.3503312-13
TACATGG177350.022.3308052
ACATGGG183700.021.1216223
AGTACTT161150.021.04720712-13
GTACTTT167950.020.72005814-15
AGAGTAC256200.020.16118610-11
AACGCGG4250.019.4356755
GTATAGG10250.019.2130931
CAGAGTA294200.018.522410-11
Produced by FastQC (version 0.11.5)