FastQCFastQC Report
Fri 27 May 2016
SRR939394_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939394_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3792942
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193240.5094725940971415No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164000.43238204011556197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113960.3004527883632283No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44840.11821957730964512No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39680.10461536190113109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144450.036.670211
GTATCAA224100.033.2313421
TATCAAC283150.026.0786612
ATCAACG285050.025.8667583
TCAACGC287250.025.6413654
CAACGCA291350.025.3050165
AACGCAG297750.024.7451736
GTACATG205150.022.32661
TACATGG203700.021.8759462
GAGTACT177100.021.81503112-13
ACGCAGA336150.021.7773697
CGCAGAG337000.021.736518
AGTACTT183700.020.83771912-13
ACATGGG204950.020.7430293
GTACTTT189900.020.35709614-15
GCAGAGT351900.020.3311169
AGAGTAC302350.019.7884610-11
CATGGGA140100.018.1227914
TACTTTT215000.017.69389214-15
ACTTTTT223650.017.6344816-17
Produced by FastQC (version 0.11.5)