FastQCFastQC Report
Fri 27 May 2016
SRR939393_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939393_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1490188
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135770.9110930969783678No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103710.6959524570054249No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86640.5814031518170862No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76430.5128883067102943No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27140.18212467151795614No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26840.18011150270972523No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26220.17595095383938136No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25370.17024697554939378No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23080.15487978697989785No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA18390.12340724794455465No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17210.11548878396551308No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17050.11441509393445659No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15650.10502030616271235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG106450.028.259271
TACATGG107500.027.4231532
GTATCAA191350.025.0804821
ACATGGG111150.024.8941763
CATGGGA72350.023.5353284
TAAGGTA1652.2883378E-623.1907524
GAGTACT105350.023.02003312-13
AGTACTT108500.022.7709412-13
GGTATCA141450.022.5718081
ATGGGAT27550.021.8818135
TATCAAC228500.021.2181422
GTACTTT116400.021.1644814-15
ATGGGAG25500.020.6148995
AACGCAG237450.020.4468926
CATGGGG34700.020.4005134
ATCAACG234900.020.4000173
CAACGCA236250.020.3525225
TCAACGC236000.020.2470744
ACTTTTT134050.020.018616-17
GCACCGT3403.274181E-1119.9517446
Produced by FastQC (version 0.11.5)