FastQCFastQC Report
Fri 27 May 2016
SRR939393_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939393_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1490188
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191731.2866161853403733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162031.0873124733255133No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA132240.8874048106681841No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123640.8296939714988981No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43730.29345290661312534No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40250.2701001484376468No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39060.2621145788316642No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38850.26070536066590255No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32800.22010645636657925No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT25840.17340094001562217No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25030.16796538423339874No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23600.1583692795808314No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA22480.150853449363436No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18800.1261585786491369No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17280.11595852335410028No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15560.10441635552024307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG122450.025.077051
TACATGG124200.024.1854972
ACATGGG122700.023.281793
GGTATCA157000.023.210631
CATGGGA76200.022.754634
GTATCAA204800.022.0970171
TATACGT1754.1446365E-621.6568645
GAGTACT122800.021.00669512-13
AGTACTT125400.021.0056212-13
ATGGGAT29900.020.7560045
GTGTACG1150.003781351720.597569
ATGGGAG26000.020.407435
GTATAGG4200.020.3087621
CTATACG1708.119379E-519.5071034
TACCTGG8750.019.493142
GTACTTT133850.019.16630214-15
TATAAGC8950.019.0556223
CGCATAG1654.838512E-1019.01417566-67
CATGGGG42050.018.5892374
TACTTTT143300.018.19991514-15
Produced by FastQC (version 0.11.5)