FastQCFastQC Report
Fri 27 May 2016
SRR939392_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939392_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479830
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133540.9024009514606407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96360.6511558760127852No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84190.5689166998912037No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75460.5099234371515647No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26580.17961522607326516No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25870.1748173776717596No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25470.1721143644878128No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23510.15886959988647345No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22710.15346357351857984No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17390.11751349817208734No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17090.11548623828412724No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16590.11210747180419373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG106000.029.0139351
TACATGG108800.027.6608472
GTATCAA190750.026.4860361
ACATGGG111400.025.7306773
ATCGACG1551.266335E-624.8226623
CATGGGA71150.024.259884
GAGTACT109550.023.72816712-13
ATGGGAG24450.023.396965
GGTATCA140350.023.128311
TATCAAC224000.022.5520572
AGTACTT114550.022.48272912-13
TAAGGTG4750.022.4535665
TCAACGC235750.021.8639664
ATCAACG232400.021.8533463
GTACTTT122750.021.59501514-15
CAACGCA236700.021.546355
AACGCAG240900.021.0525516
ATGGGAT27600.020.9023045
CATGGGG38150.020.3726734
ACTTTTT142950.020.32582316-17
Produced by FastQC (version 0.11.5)