##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939392_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1479830 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.135426366542102 31.0 27.0 31.0 10.0 33.0 2 27.086219363034942 31.0 26.0 31.0 16.0 34.0 3 27.350681497199 31.0 26.0 31.0 16.0 34.0 4 31.188520978761073 35.0 30.0 37.0 19.0 37.0 5 31.630250096294844 35.0 32.0 37.0 19.0 37.0 6 31.505768905887837 35.0 31.0 37.0 17.0 37.0 7 31.48906496016434 35.0 31.0 37.0 17.0 37.0 8 31.42595230533237 35.0 30.0 37.0 17.0 37.0 9 32.45344667968618 35.0 31.0 39.0 17.0 39.0 10-11 31.57660069061987 35.0 30.0 38.5 14.0 39.0 12-13 31.312811944615262 35.0 29.0 38.0 15.5 39.0 14-15 32.03158538480771 36.0 29.0 39.0 16.5 40.0 16-17 31.975437381320827 36.0 29.5 39.0 16.5 40.0 18-19 32.058497259820385 36.0 30.0 39.0 16.5 40.0 20-21 32.0270754073103 36.0 29.5 39.0 16.0 40.0 22-23 32.14401890757722 36.0 30.0 39.0 16.0 40.0 24-25 32.11150706500071 36.0 30.0 39.0 15.5 41.0 26-27 32.153773406404795 36.0 30.0 39.0 16.0 41.0 28-29 31.709028064034385 36.0 30.0 39.0 10.5 40.5 30-31 31.343807396795576 35.5 29.0 39.0 10.0 40.0 32-33 31.121720062439607 35.0 29.0 39.0 9.5 40.0 34-35 30.610428224863668 35.0 27.0 38.0 9.0 40.0 36-37 30.16598291695668 35.0 26.0 38.0 9.0 40.0 38-39 29.739598805268173 35.0 25.0 38.0 8.0 40.0 40-41 29.536738341566263 34.5 25.0 38.0 7.5 40.0 42-43 29.19407127845766 34.0 24.5 38.0 3.5 40.0 44-45 28.747490590135353 34.0 24.0 38.0 2.0 40.0 46-47 28.049354317725683 33.0 23.0 38.0 2.0 40.0 48-49 27.635064162775457 33.0 22.0 38.0 2.0 40.0 50-51 26.93227465316962 32.5 20.0 37.5 2.0 39.5 52-53 27.025752958110054 33.0 20.0 37.5 2.0 39.5 54-55 27.153013183946804 33.0 20.0 38.0 2.0 40.0 56-57 26.696461417865564 33.0 18.5 38.0 2.0 40.0 58-59 26.311733442354864 32.5 18.0 37.0 2.0 40.0 60-61 25.70759614280019 31.5 14.0 37.0 2.0 39.0 62-63 25.145876553387886 31.0 11.0 36.0 2.0 39.0 64-65 24.611445233574127 31.0 8.0 36.0 2.0 39.0 66-67 24.221640999303975 30.5 7.5 35.0 2.0 38.5 68-69 23.5412442645439 30.0 4.0 35.0 2.0 38.0 70-71 22.92290905036389 29.0 2.0 34.5 2.0 37.5 72-73 22.438072616449187 29.0 2.0 34.0 2.0 37.0 74-75 21.72577492009217 28.5 2.0 34.0 2.0 36.0 76-77 20.957343411067487 27.0 2.0 34.0 2.0 36.0 78-79 20.453079069893164 26.0 2.0 33.0 2.0 35.0 80-81 19.940789144699053 26.0 2.0 33.0 2.0 35.0 82-83 19.230414980099063 25.5 2.0 33.0 2.0 35.0 84-85 18.60334565456843 24.5 2.0 32.0 2.0 35.0 86-87 17.86304271436584 22.5 2.0 32.0 2.0 34.0 88-89 17.083972821202437 19.5 2.0 31.5 2.0 34.0 90-91 16.285489887351925 15.5 2.0 31.0 2.0 34.0 92-93 15.480212929863566 4.5 2.0 31.0 2.0 34.0 94-95 14.450906860923215 2.0 2.0 30.5 2.0 34.0 96-97 13.378803646364783 2.0 2.0 29.5 2.0 34.0 98-99 12.387849279984863 2.0 2.0 29.0 2.0 34.0 100-101 11.098526182061452 2.0 2.0 26.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.9139606452512155 1101 2 3.624273109294048 1101 3 3.391758860581092 1101 4 3.148213062797332 1101 5 2.323062685076632 1101 6 2.590893680314096 1101 7 2.5763152568984644 1101 8 2.5717124178769204 1101 9 2.610873053634222 1101 10-11 3.524764329932264 1101 12-13 3.271977027662661 1101 14-15 2.782209868880919 1101 16-17 2.666643430687202 1101 18-19 2.873970991715556 1101 20-21 2.782952031096187 1101 22-23 2.7389139921765597 1101 24-25 2.9036142387047086 1101 26-27 2.688745238283051 1101 28-29 2.956699074608892 1101 30-31 2.938278781891512 1101 32-33 2.7925644742496054 1101 34-35 2.9853326433102296 1101 36-37 2.7884293401734688 1101 38-39 2.6647047703639615 1101 40-41 2.6535226767858013 1101 42-43 2.8406772810730168 1101 44-45 2.616482431270935 1101 46-47 2.3451141921754335 1101 48-49 2.1313004134153175 1101 50-51 2.4282910079211746 1101 52-53 2.1293560548054167 1101 54-55 2.097983551245214 1101 56-57 2.231007015508361 1101 58-59 2.3073921429285313 1101 60-61 2.1689278205843934 1101 62-63 2.339235085324308 1101 64-65 2.2008299644435994 1101 66-67 1.8636926508019585 1101 68-69 2.1156291035843857 1101 70-71 1.7343471018070957 1101 72-73 1.7879448230871766 1101 74-75 1.910845168931207 1101 76-77 2.0222667374063015 1101 78-79 1.7104591697244373 1101 80-81 1.9996423439053501 1101 82-83 1.4705378470770505 1101 84-85 1.4855170226285423 1101 86-87 1.406110927490598 1101 88-89 1.6159443779479865 1101 90-91 1.6537699164844089 1101 92-93 1.3657358724436879 1101 94-95 1.441202703423615 1101 96-97 1.7882383430128819 1101 98-99 1.5869227274730378 1101 100-101 1.2539397007036914 1102 1 3.644300315749007 1102 2 2.4839672744754715 1102 3 2.801517107704644 1102 4 3.011005297658947 1102 5 2.1622875228719103 1102 6 2.711641161602408 1102 7 2.4688372373321847 1102 8 2.562088920406236 1102 9 2.67300788397138 1102 10-11 1.9677686403350556 1102 12-13 3.216530028100909 1102 14-15 3.1943681286932843 1102 16-17 2.9500414666110117 1102 18-19 3.018049161685628 1102 20-21 3.1189938048677988 1102 22-23 3.108251653497721 1102 24-25 2.993780651767569 1102 26-27 2.9218081758185868 1102 28-29 3.156883510610214 1102 30-31 3.3278023411856665 1102 32-33 2.9146903120817775 1102 34-35 2.984880432026358 1102 36-37 2.94521942276452 1102 38-39 3.114929634709199 1102 40-41 2.8987087730214647 1102 42-43 2.786292452201284 1102 44-45 2.704049189759072 1102 46-47 2.7294946454763007 1102 48-49 2.668627929204785 1102 50-51 2.5758329128909487 1102 52-53 2.721432168360618 1102 54-55 2.6154965236577397 1102 56-57 2.603169316431096 1102 58-59 2.349646572202726 1102 60-61 2.7342401980709994 1102 62-63 2.8413871834777744 1102 64-65 2.749488622677724 1102 66-67 2.5475061073546783 1102 68-69 2.149739074886295 1102 70-71 2.2651729813261525 1102 72-73 2.045544058724257 1102 74-75 2.209927850669974 1102 76-77 2.236965035071318 1102 78-79 1.624966066334423 1102 80-81 1.7380590630685226 1102 82-83 1.714544021838524 1102 84-85 1.502102815367163 1102 86-87 1.385344033701113 1102 88-89 1.3375527329781498 1102 90-91 1.1914084460581282 1102 92-93 1.1521385359905612 1102 94-95 1.059698093213191 1102 96-97 1.018459745519543 1102 98-99 0.7100712558640794 1102 100-101 0.24214376160584372 1103 1 3.927682037253337 1103 2 3.6604135167126337 1103 3 3.2920222883651427 1103 4 3.1757405151406672 1103 5 2.683265697903888 1103 6 2.594076595335764 1103 7 2.473529258856761 1103 8 2.3205028214034975 1103 9 2.6812474652234073 1103 10-11 3.3248809878817305 1103 12-13 2.474616187588854 1103 14-15 3.3107302964833423 1103 16-17 2.755898881894339 1103 18-19 2.4474219707956486 1103 20-21 2.5667819829308343 1103 22-23 2.9412040659062946 1103 24-25 2.857199772163046 1103 26-27 2.6469428063898484 1103 28-29 2.3124394172971634 1103 30-31 2.9747069933820995 1103 32-33 2.9148252144967053 1103 34-35 2.7378849840483994 1103 36-37 3.136639663155865 1103 38-39 3.3622356640655937 1103 40-41 3.2618693021379563 1103 42-43 3.057805139824378 1103 44-45 2.9299548369032884 1103 46-47 2.734538194830197 1103 48-49 2.577787633275779 1103 50-51 2.6809266513800694 1103 52-53 2.685754557268311 1103 54-55 2.8095820212063884 1103 56-57 3.00410201201921 1103 58-59 2.9197186923249987 1103 60-61 2.788106018760203 1103 62-63 2.923003994017698 1103 64-65 2.798133346124377 1103 66-67 2.52847942997737 1103 68-69 2.509708499270946 1103 70-71 2.497143970171045 1103 72-73 2.5198580612551176 1103 74-75 2.490518417423221 1103 76-77 2.4572108972710502 1103 78-79 2.309580530863581 1103 80-81 2.3403422779459824 1103 82-83 2.4970723241612944 1103 84-85 2.539591754728356 1103 86-87 2.136807911795543 1103 88-89 1.9111978981707445 1103 90-91 1.9989862918874355 1103 92-93 1.8226118633705202 1103 94-95 1.8363803976862432 1103 96-97 1.7567127014795112 1103 98-99 1.5712515738396071 1103 100-101 1.4183907084617697 1104 1 3.8957922333217674 1104 2 3.6817440606344576 1104 3 2.940321601702923 1104 4 2.894279455745796 1104 5 1.7820493785046345 1104 6 2.2752110976633304 1104 7 2.3159082743948964 1104 8 2.325846360511843 1104 9 2.4292597411389707 1104 10-11 3.3178709476106114 1104 12-13 3.3487959368907276 1104 14-15 2.9914077061417004 1104 16-17 2.9392586287539046 1104 18-19 2.6710533430868146 1104 20-21 2.467791478381038 1104 22-23 2.125320993062669 1104 24-25 2.322456200311766 1104 26-27 2.365448031168736 1104 28-29 2.2947879242060623 1104 30-31 2.0152381132574604 1104 32-33 2.1752325016777156 1104 34-35 2.832342638983519 1104 36-37 3.2721063304487608 1104 38-39 3.2116983579232112 1104 40-41 3.232902375182878 1104 42-43 2.9959659532446885 1104 44-45 3.2777963195139748 1104 46-47 3.3466413042009826 1104 48-49 2.725975231487798 1104 50-51 2.6752271670812426 1104 52-53 3.000829288984267 1104 54-55 2.7722778917054107 1104 56-57 3.0905668355093106 1104 58-59 3.242025552640161 1104 60-61 3.2354147242200852 1104 62-63 3.0421854140142344 1104 64-65 3.178621309742148 1104 66-67 2.981107855404673 1104 68-69 3.1459769658147785 1104 70-71 3.152261596469522 1104 72-73 2.803897458785176 1104 74-75 2.9248000656809374 1104 76-77 2.94488818213053 1104 78-79 2.8257641274599266 1104 80-81 3.0531646247285202 1104 82-83 3.159198648807461 1104 84-85 2.785788743720559 1104 86-87 2.9541536811806353 1104 88-89 3.1077433846783755 1104 90-91 2.820735583016381 1104 92-93 2.6405103374693812 1104 94-95 2.478301381208764 1104 96-97 2.392680073953173 1104 98-99 2.3765002389612704 1104 100-101 1.9133475924618804 1105 1 3.653442275587196 1105 2 3.275568859317559 1105 3 3.0940431837645868 1105 4 2.846506176393568 1105 5 0.0160352307091145 1105 6 1.923286908492173 1105 7 2.1841691633035545 1105 8 2.176582668738032 1105 9 2.6906380389545106 1105 10-11 2.850217346151343 1105 12-13 3.339050419250473 1105 14-15 3.2505170178825935 1105 16-17 3.0806342571740686 1105 18-19 3.1137658936940973 1105 20-21 3.0749187640508353 1105 22-23 2.8990741629696792 1105 24-25 2.27896730054756 1105 26-27 2.66433355859677 1105 28-29 2.845239496924833 1105 30-31 2.8134451693592553 1105 32-33 2.752126660150818 1105 34-35 3.23299886534825 1105 36-37 3.2363386568268417 1105 38-39 3.4318789870107196 1105 40-41 3.355756848644642 1105 42-43 3.4288440063656545 1105 44-45 3.235137156814325 1105 46-47 3.2743496282655826 1105 48-49 3.4763845387796977 1105 50-51 3.6002816672369597 1105 52-53 3.6690701574275657 1105 54-55 3.351530016099236 1105 56-57 3.587069927558961 1105 58-59 3.4515374981844964 1105 60-61 3.594083585722956 1105 62-63 3.3430396205364588 1105 64-65 3.386255780534512 1105 66-67 3.4231285877088062 1105 68-69 3.496137129537697 1105 70-71 3.553387191522244 1105 72-73 3.3326935614679165 1105 74-75 3.201207059496838 1105 76-77 3.259921327123191 1105 78-79 3.1543889439389936 1105 80-81 3.1993985527438227 1105 82-83 3.506509232960152 1105 84-85 3.299066761249591 1105 86-87 3.016099225514843 1105 88-89 2.918047250852691 1105 90-91 2.9874059571766356 1105 92-93 2.9344485854562112 1105 94-95 2.953189044153108 1105 96-97 2.886360280386416 1105 98-99 2.849021038000288 1105 100-101 2.6469664028013398 1106 1 3.0113391336787956 1106 2 2.586606020792889 1106 3 2.138421525319167 1106 4 1.4321601848323837 1106 5 1.811438820952045 1106 6 2.2258667045481175 1106 7 2.0189462978859503 1106 8 0.6567856854551657 1106 9 1.8668581187773157 1106 10-11 1.1359507137250198 1106 12-13 1.7139542074454006 1106 14-15 1.8073631253616114 1106 16-17 1.7327662537072257 1106 18-19 1.8434060847266913 1106 20-21 1.836227173265769 1106 22-23 2.5012425398702867 1106 24-25 2.686573650705032 1106 26-27 2.653065809792512 1106 28-29 2.377088368598443 1106 30-31 2.420446606740434 1106 32-33 2.4505849529129087 1106 34-35 2.6826561052197064 1106 36-37 1.7837859677113492 1106 38-39 2.68117902160774 1106 40-41 2.1320257922943036 1106 42-43 2.1301133958531544 1106 44-45 2.2720687581568306 1106 46-47 2.561698177152593 1106 48-49 2.3030361896280347 1106 50-51 2.125837627018818 1106 52-53 2.191520232506658 1106 54-55 2.442484323071067 1106 56-57 2.6385385173917157 1106 58-59 2.598144672250289 1106 60-61 2.661127252863995 1106 62-63 2.9212916508819653 1106 64-65 2.6587554327809997 1106 66-67 2.2688476528046984 1106 68-69 2.5953586966755253 1106 70-71 2.7180126342392157 1106 72-73 2.69866846614779 1106 74-75 2.838710938286013 1106 76-77 2.9722667551453448 1106 78-79 2.8091232067550465 1106 80-81 2.675117510838632 1106 82-83 2.6706025236357185 1106 84-85 2.553895720022073 1106 86-87 2.6133113123569203 1106 88-89 2.7709007913401145 1106 90-91 2.2642311281108594 1106 92-93 2.4239355509412324 1106 94-95 2.5247722321433077 1106 96-97 2.5085881498878972 1106 98-99 2.0196697498856118 1106 100-101 1.6506716718912244 1107 1 3.059832507768135 1107 2 3.3565663663010064 1107 3 -0.18813099699413272 1107 4 0.9635112525356995 1107 5 1.4637284394917423 1107 6 2.0755651651827733 1107 7 2.0638781111304247 1107 8 1.900278340500865 1107 9 2.053537146875094 1107 10-11 1.677395356543915 1107 12-13 2.339450653533948 1107 14-15 2.4821892162426096 1107 16-17 1.262333863697961 1107 18-19 0.1750261435964795 1107 20-21 -1.1930685005393187 1107 22-23 0.8248907760715376 1107 24-25 1.474725417869525 1107 26-27 1.5121409054865076 1107 28-29 2.3096952383719866 1107 30-31 2.8796448960285232 1107 32-33 1.3321295886943538 1107 34-35 0.8590197255891425 1107 36-37 1.029046207963713 1107 38-39 2.1286433218558827 1107 40-41 2.4142683976188977 1107 42-43 1.589423557622677 1107 44-45 1.440950691190924 1107 46-47 2.369907628445798 1107 48-49 1.7242316838251988 1107 50-51 1.9972911737398285 1107 52-53 1.5812998303317798 1107 54-55 2.204420638752648 1107 56-57 2.7753384825104526 1107 58-59 2.6419589080904835 1107 60-61 2.9422025596814443 1107 62-63 2.59951770902353 1107 64-65 2.041492992561068 1107 66-67 2.4903457955302706 1107 68-69 2.7418772250532726 1107 70-71 0.9044587380935951 1107 72-73 2.0111131992296087 1107 74-75 2.6787545169203177 1107 76-77 2.6184144788367654 1107 78-79 2.6707794013925117 1107 80-81 2.304550912098641 1107 82-83 2.327550818278386 1107 84-85 2.6923404974704184 1107 86-87 2.970216081911083 1107 88-89 2.6976296705805574 1107 90-91 2.676642433312759 1107 92-93 2.8601529377224555 1107 94-95 2.8841032002820217 1107 96-97 2.6931387323132476 1107 98-99 2.5153897191044248 1107 100-101 2.2915497236195304 1108 1 2.333202496735858 1108 2 2.4492625774736965 1108 3 1.5372772943615445 1108 4 1.6779911987007665 1108 5 1.7484623221743014 1108 6 -0.14724156288936285 1108 7 0.5056051965060249 1108 8 -0.552925064299636 1108 9 1.1492462053138226 1108 10-11 2.4056529117133927 1108 12-13 1.6281149875693366 1108 14-15 1.6388829540496772 1108 16-17 -0.04741473947325403 1108 18-19 0.08068014578999438 1108 20-21 0.9229273324300422 1108 22-23 -0.6065863602596018 1108 24-25 0.7234972276484548 1108 26-27 1.131224805801299 1108 28-29 1.9992478163986647 1108 30-31 0.5088513565436408 1108 32-33 1.0161189139136262 1108 34-35 1.049971761060668 1108 36-37 0.2989227095344411 1108 38-39 1.7103230266696414 1108 40-41 0.1634616016060484 1108 42-43 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2312 40-41 -0.31070022105129524 2312 42-43 -2.6332031232928124 2312 44-45 -3.584873174734465 2312 46-47 -1.4779843625299485 2312 48-49 -0.3837863519579976 2312 50-51 -1.4876158378670254 2312 52-53 -1.303424936322589 2312 54-55 -1.4067273136155833 2312 56-57 -0.5022935205089425 2312 58-59 -0.7682565629051119 2312 60-61 -0.3647073759020145 2312 62-63 -0.8137685735187006 2312 64-65 -2.7420778741149334 2312 66-67 0.3178150079012987 2312 68-69 -0.2973107406308202 2312 70-71 -2.9211591348459436 2312 72-73 -3.370512926151523 2312 74-75 -1.0976277988521908 2312 76-77 -0.7965487435169187 2312 78-79 -1.0314751245161666 2312 80-81 -2.33511376354987 2312 82-83 -0.8233538863766547 2312 84-85 0.8714194872460403 2312 86-87 1.1366291004981584 2312 88-89 1.5168109253248652 2312 90-91 2.1442687749706284 2312 92-93 2.1706176570503875 2312 94-95 1.5287801422138418 2312 96-97 1.188515817157338 2312 98-99 1.7481000426400328E-4 2312 100-101 0.72794819653617 2313 1 -7.55355809062139 2313 2 -4.286611430950931 2313 3 -2.014023042166837 2313 4 -0.004308155853991025 2313 5 0.47165267765705465 2313 6 -2.4300359525605764 2313 7 -1.091742021477586 2313 8 -2.131538488481098 2313 9 0.5280944312217315 2313 10-11 -1.6193863776117432 2313 12-13 -2.4926584256505606 2313 14-15 -3.8571940820923487 2313 16-17 -3.431297396739545 2313 18-19 -1.063113334569497 2313 20-21 -3.080095402439156 2313 22-23 -1.7482472139745973 2313 24-25 -2.924716950555883 2313 26-27 -2.3426475334757733 2313 28-29 -3.082000639536048 2313 30-31 -4.631781590851219 2313 32-33 -6.250422826193454 2313 34-35 -5.153706799320332 2313 36-37 -2.362553562204443 2313 38-39 -1.6761380898738096 2313 40-41 -3.9077442017957758 2313 42-43 -3.2036285761758343 2313 44-45 -3.230923017038382 2313 46-47 -1.88220984208116 2313 48-49 -2.977637973411195 2313 50-51 -3.8305901983066697 2313 52-53 -1.0034227593274387 2313 54-55 -2.313801595411281 2313 56-57 -2.7971763573513186 2313 58-59 -2.1692400204632314 2313 60-61 -2.3932113856549506 2313 62-63 -4.011210808313066 2313 64-65 -5.206327531918504 2313 66-67 -4.173050796675287 2313 68-69 -3.1833137503266116 2313 70-71 -2.201210171775948 2313 72-73 -2.118764526923268 2313 74-75 -3.2941710026815265 2313 76-77 -3.032322760535582 2313 78-79 -3.247633394784671 2313 80-81 -3.510722777942526 2313 82-83 -1.7036511278516748 2313 84-85 -2.032899875220931 2313 86-87 -3.247337738423248 2313 88-89 -3.619138339803211 2313 90-91 -3.4266723536380823 2313 92-93 -1.7478514935677456 2313 94-95 -0.730258809671847 2313 96-97 -1.0453138710697445 2313 98-99 -0.6576362485868401 2313 100-101 -0.4663982831266136 2314 1 -7.51087599206986 2314 2 -3.927739714269247 2314 3 -24.92607515943068 2314 4 -13.269238504457498 2314 5 -6.6075503163134215 2314 6 -5.229612499791848 2314 7 -4.295636955291432 2314 8 -3.606169440036993 2314 9 -3.71908624657992 2314 10-11 -14.344297320851052 2314 12-13 -13.122962674039222 2314 14-15 -4.910170379826081 2314 16-17 -2.897024058323858 2314 18-19 -3.4421260866659686 2314 20-21 -4.649240873365763 2314 22-23 -4.632439119165529 2314 24-25 -5.0847869484767365 2314 26-27 -3.9794823799654786 2314 28-29 -3.3105958297779203 2314 30-31 -3.9292062218027795 2314 32-33 -3.9227178570341223 2314 34-35 -3.1322749606620732 2314 36-37 -3.9419655103643976 2314 38-39 -1.1705860765672753 2314 40-41 -2.8808514857378356 2314 42-43 -4.463206856028908 2314 44-45 -5.317524306109004 2314 46-47 -4.682759776241532 2314 48-49 -3.2521323943689886 2314 50-51 -2.6184656574514484 2314 52-53 -0.8797010876939275 2314 54-55 -2.982526385764043 2314 56-57 -2.6078513868058906 2314 58-59 -0.8265690106912409 2314 60-61 -3.6308415055523824 2314 62-63 -4.887890758413562 2314 64-65 -4.38785223910542 2314 66-67 -5.093462121091395 2314 68-69 -4.614187684764204 2314 70-71 -5.155095749432057 2314 72-73 -4.245275719660118 2314 74-75 -3.7387174022934175 2314 76-77 -3.5712180408737915 2314 78-79 -4.763493468247837 2314 80-81 -6.603988076993051 2314 82-83 -6.202197804683056 2314 84-85 -4.8714468985766715 2314 86-87 -4.059067588031677 2314 88-89 -3.470181378128805 2314 90-91 -5.497555644233412 2314 92-93 -5.456606431539884 2314 94-95 -6.003513712991548 2314 96-97 -5.3521528758517825 2314 98-99 -5.8895047079395315 2314 100-101 -4.908923059618401 2315 1 -24.499812162282627 2315 2 -24.563484395120312 2315 3 -13.225649627515788 2315 4 -28.753493823606433 2315 5 -15.504571592909167 2315 6 -12.042378457238655 2315 7 -9.216488019121218 2315 8 -8.112552418760398 2315 9 -5.721639438069282 2315 10-11 -29.199190937872327 2315 12-13 -28.934026503826455 2315 14-15 -23.596553358549485 2315 16-17 -14.195747462579178 2315 18-19 -9.46467927815533 2315 20-21 -7.144560022301931 2315 22-23 -3.749034863846383 2315 24-25 -5.649893331455459 2315 26-27 -5.569695145922928 2315 28-29 -17.369744765948134 2315 30-31 -16.501546647334692 2315 32-33 -9.480590197459659 2315 34-35 -7.336104747896115 2315 36-37 -4.888774021002693 2315 38-39 -5.335692459545996 2315 40-41 -8.780000421908046 2315 42-43 -8.153419621986355 2315 44-45 -4.951141327385599 2315 46-47 -3.852547010284084 2315 48-49 -4.810004734794521 2315 50-51 -2.9444231042599576 2315 52-53 -6.768211725991797 2315 54-55 -6.365505109168296 2315 56-57 -7.935936493188869 2315 58-59 -7.331249861755072 2315 60-61 -4.765735122573659 2315 62-63 -3.3202311839454737 2315 64-65 -4.286150111445849 2315 66-67 -6.776866376410542 2315 68-69 -6.420570663487606 2315 70-71 -7.12743617496397 2315 72-73 -6.488254443064376 2315 74-75 -6.781696125697675 2315 76-77 -6.563983998320598 2315 78-79 -6.717110489524433 2315 80-81 -7.7963285025249665 2315 82-83 -7.177091421704333 2315 84-85 -6.546340515597949 2315 86-87 -6.427578226329548 2315 88-89 -6.0714579738734855 2315 90-91 -5.706491814446176 2315 92-93 -5.562563878348394 2315 94-95 -5.7226626491617605 2315 96-97 -5.717259258830505 2315 98-99 -5.632908963258681 2315 100-101 -5.256582634086486 2316 1 -1.0401967776672407 2316 2 1.633630989495071 2316 3 2.0758479174923963 2316 4 2.2109292533166425 2316 5 -1.3073375503559745 2316 6 -2.1551894228687694 2316 7 -2.613537937288161 2316 8 -3.8855475087767637 2316 9 -2.991684446252588 2316 10-11 -2.551114014795406 2316 12-13 -3.3498918884418423 2316 14-15 -1.6215983667327905 2316 16-17 -1.7642621925300759 2316 18-19 -3.91219564476744 2316 20-21 -1.2535575673101107 2316 22-23 -2.0964509849593114 2316 24-25 -1.5595312201624978 2316 26-27 -2.135296618918016 2316 28-29 -1.7501007397615531 2316 30-31 1.0240220924361765 2316 32-33 -1.3829694936953345 2316 34-35 -7.322696400923938 2316 36-37 -2.734852171575529 2316 38-39 -0.6373147999715272 2316 40-41 -0.8654709620062455 2316 42-43 -1.1309932717408522 2316 44-45 -0.4552104763217706 2316 46-47 0.5954664921709067 2316 48-49 1.3200975565311772 2316 50-51 1.885044980846427 2316 52-53 1.3727314000311175 2316 54-55 -0.11230134484751275 2316 56-57 1.1863302825885498 2316 58-59 -2.3496459337681657 2316 60-61 -2.3969667101350325 2316 62-63 -3.8770417404102844 2316 64-65 -4.235859604080872 2316 66-67 -1.9540533649539178 2316 68-69 -1.6676942314090475 2316 70-71 -2.3636616250458005 2316 72-73 -2.1122028882362223 2316 74-75 -1.6209453073670694 2316 76-77 -1.387637844474444 2316 78-79 -1.7960548430432546 2316 80-81 -2.0416517431141656 2316 82-83 -2.718971536270619 2316 84-85 -2.00097022099893 2316 86-87 -1.6081981117632633 2316 88-89 -3.270488252105892 2316 90-91 -3.4271280664920045 2316 92-93 -3.6783842547804735 2316 94-95 -5.080612730994822 2316 96-97 -5.081539127897608 2316 98-99 -5.263663873242315 2316 100-101 -4.980807265837713 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20605.0 3 7604.0 4 6744.0 5 7437.0 6 9063.0 7 11577.0 8 14314.0 9 16941.0 10 20092.0 11 22909.0 12 26077.0 13 28660.0 14 31198.0 15 33812.0 16 35790.0 17 35518.0 18 32622.0 19 32275.0 20 33986.0 21 36844.0 22 39775.0 23 42735.0 24 46348.0 25 50656.0 26 54675.0 27 58554.0 28 62563.0 29 66850.0 30 71944.0 31 76434.0 32 80868.0 33 84777.0 34 86465.0 35 80469.0 36 66161.0 37 39177.0 38 7264.0 39 47.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.004946662411776 19.208153609999837 14.622381991950931 20.164517735637453 2 16.064544854630046 21.719525005487682 39.3937442483189 22.82218589156337 3 19.95315108094263 27.68431413878276 29.537875113660473 22.824659666614142 4 12.438577556083882 17.72314065645908 36.94864684830505 32.88963493915198 5 12.822382056665868 39.00357529163468 32.693882745055035 15.480159906644412 6 29.546933358945605 36.68146136041741 17.806863637654907 15.96474164298208 7 27.48678828724932 35.36092173890693 20.175010805432546 16.977279168411204 8 27.166875801165517 32.68728248039153 19.617605178869184 20.528236539573772 9 28.092227729737335 17.383913786945744 20.70409311789089 33.819765365426036 10-11 24.994973309562674 27.539114525708985 25.79941563219955 21.666496532528786 12-13 27.412093083127836 25.791255543128127 26.83560433405604 19.961047039687994 14-15 24.055516806017852 26.139366579757528 27.347378701307168 22.457737912917448 16-17 20.99710789134437 29.96636469517202 27.528464544614632 21.50806286886898 18-19 20.569054190660786 27.184818998640758 32.31982808447373 19.92629872622473 20-21 21.98053945947153 25.769265769360487 32.31936179332233 19.930832977845647 22-23 21.430188231098647 26.073625755311415 32.307078603138685 20.189107410451253 24-25 21.311933059626213 26.39651708650469 32.56463885853777 19.726910995331323 26-27 20.875821912060484 26.41934636842775 32.936645434784374 19.768186284727392 28-29 20.231283979750504 26.9037578295902 33.03602095614003 19.828937234519266 30-31 20.24471390414063 26.694179040973058 32.882804664511845 20.178302390374462 32-33 20.162625453147463 27.044409128197078 32.52306009567875 20.269905322976708 34-35 21.126563741366684 26.742261315850378 32.239193723244 19.891981219538934 36-37 21.099123355170228 26.43816137017919 32.219767831725875 20.24294744292471 38-39 20.71540897840643 26.940376364971897 32.43276712905081 19.911447527570864 40-41 20.462906674758635 27.01094073545585 32.56770850776569 19.958444082019827 42-43 20.394389201133688 27.281399885115565 31.760708846439833 20.563502067310914 44-45 20.504041056730415 27.378776755719898 31.39909537164528 20.718086815904407 46-47 20.93239951278928 27.646027879280012 30.689707673568815 20.73186493436189 48-49 21.092410955242013 27.851301895188513 30.291139261923817 20.765147887645657 50-51 20.83398764833772 28.20900896998183 30.25206244601901 20.70494093566143 52-53 20.976522713125 28.447402475850623 29.852918726010984 20.72315608501339 54-55 20.34014482760722 28.147688549899453 30.02727237954812 21.484894242945213 56-57 20.955067472222993 28.64606844611866 29.443695091471067 20.95516899018727 58-59 21.17039680093436 27.863830616525238 29.618288997916846 21.34748358462356 60-61 20.95195409025331 28.076556836652028 30.24705410767412 20.724434965420542 62-63 21.057997802518987 28.135571318001485 30.024775786573716 20.78165509290582 64-65 20.774825478557634 28.254054915268682 29.942220269282434 21.028899336891246 66-67 20.701842926248787 28.494813334501444 29.480629875342107 21.322713863907662 68-69 20.691907418256307 28.38106422733318 29.726907668557683 21.20012068585283 70-71 20.77385372388561 28.48657892377668 29.575285395561885 21.16428195677583 72-73 20.857320244078622 28.2220729765987 29.49182992991283 21.42877684940985 74-75 20.484638365359793 29.0281888541906 29.11007516283363 21.377097617615977 76-77 20.812638867971145 28.50720632755665 29.23428644919357 21.445868355278634 78-79 20.54259551822776 28.64697345674249 29.298844832884562 21.511586192145188 80-81 20.549854369884923 29.05600007581024 29.116851330029718 21.277294224275114 82-83 20.73889325067006 28.76495790129139 28.847733979478573 21.648414868559982 84-85 20.43199295103146 28.714463311323353 28.766910993914042 22.08663274373115 86-87 20.65307942755476 28.66983699238188 29.043366509639217 21.633717070424137 88-89 20.74631037075284 28.463182883395604 28.67249139385392 22.118015351997634 90-91 20.452732018730284 28.514889277054046 29.037220316362877 21.995158387852793 92-93 20.749163503901055 28.673012160787252 28.851290973421268 21.726533361890425 94-95 20.45698129380087 28.35980709507196 28.825007769142385 22.358203841984782 96-97 20.634179617081852 28.145482490068197 28.59065742011818 22.629680472731767 98-99 20.54118442161406 28.45040959932606 28.119973968755733 22.888432010304147 100-101 20.580445802751115 28.228642569367114 28.63261236510633 22.558299262775446 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7915.0 1 5687.0 2 3767.5 3 4061.0 4 3625.0 5 3423.0 6 4515.0 7 7221.0 8 10357.5 9 11442.5 10 9721.5 11 7469.0 12 6374.0 13 5905.0 14 5624.5 15 5329.0 16 5060.0 17 4990.0 18 5090.0 19 5180.0 20 5340.5 21 5680.5 22 6291.5 23 7050.0 24 7811.0 25 8822.5 26 10242.0 27 11869.5 28 13274.5 29 14724.5 30 16737.5 31 19146.0 32 22278.5 33 25526.5 34 28608.5 35 31179.0 36 33874.5 37 36912.0 38 38141.5 39 38479.5 40 38914.0 41 39800.0 42 41009.0 43 42400.0 44 44314.5 45 45871.0 46 46974.0 47 50141.0 48 57449.0 49 58338.5 50 52038.5 51 47655.0 52 44615.0 53 43207.5 54 42114.0 55 39406.0 56 36224.5 57 33109.0 58 29954.5 59 26883.0 60 23740.0 61 20425.5 62 16810.0 63 13365.0 64 10455.0 65 8096.0 66 6219.5 67 4832.0 68 3612.0 69 2579.0 70 1905.5 71 1490.0 72 1192.5 73 973.5 74 802.5 75 621.5 76 450.5 77 325.0 78 215.0 79 152.0 80 116.5 81 81.0 82 50.0 83 35.5 84 29.0 85 18.5 86 7.5 87 5.0 88 4.0 89 3.5 90 3.0 91 2.5 92 2.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.073913895515025 2 2.4115607873877405 3 0.9353101369751932 4 0.8881425569153214 5 0.16677591344951784 6 0.0927133522093754 7 0.09507848874532886 8 0.10237662434198523 9 0.09771392659967698 10-11 1.695836683943426 12-13 1.3076164153990661 14-15 0.5008007676557442 16-17 0.08946973638863924 18-19 0.09659893366129893 20-21 0.12254786022718826 22-23 0.13822533669407971 24-25 0.14978071805545232 26-27 0.1471790678659035 28-29 0.5466506287884418 30-31 0.18086536967084058 32-33 0.16123473642242692 34-35 0.14353000006757533 36-37 0.13376536494056748 38-39 0.41406783211585113 40-41 0.12308846286397761 42-43 0.12318982585837564 44-45 0.12670374299750647 46-47 0.13717791908530033 48-49 0.15356493651297784 50-51 0.16610016015353113 52-53 0.18390625950278072 54-55 0.18208172560361663 56-57 0.1526526695633958 58-59 0.13673867944290896 60-61 0.11572275193772258 62-63 0.11987863470804079 64-65 0.11599305325611727 66-67 0.11247913611698641 68-69 0.10893143131305624 70-71 0.12416966813755634 72-73 0.13700898076130366 74-75 0.14673982822351214 76-77 0.16775575572869858 78-79 0.16677591344951784 80-81 0.16603258482393246 82-83 0.15880202455687478 84-85 0.14643573924031814 86-87 0.14657088989951547 88-89 0.14238121946439794 90-91 0.15021995769784366 92-93 0.14113107586682255 94-95 0.19144090875303246 96-97 0.19478588756816662 98-99 0.21238926092862018 100-101 0.23529729766256935 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1479830.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.9667760582387 #Duplication Level Percentage of deduplicated Percentage of total 1 84.06108373527395 50.40872183566025 2 8.800083688993459 10.554252957432597 3 2.9317971114174504 5.274312624856841 4 1.3260996778346805 3.1808768964645906 5 0.7280112547651798 2.182824394119045 6 0.41941195858461683 1.50904697979546 7 0.2996318010299675 1.257756717860356 8 0.21292074782750053 1.0214536642499552 9 0.153020761456237 0.8258545561056596 >10 0.939591789427908 10.727625025681977 >50 0.08371475252242869 3.4414262514200313 >100 0.04108658556363012 4.509161095255712 >500 0.0015896468599704166 0.6209388806365829 >1k 0.0014673663322803847 1.6292679486141044 >5k 3.668415830700962E-4 1.8772621968796834 >10k+ 1.2228052769003205E-4 0.979217974967044 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13354 0.9024009514606407 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9636 0.6511558760127852 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8419 0.5689166998912037 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7546 0.5099234371515647 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2658 0.17961522607326516 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2587 0.1748173776717596 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2547 0.1721143644878128 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2351 0.15886959988647345 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2271 0.15346357351857984 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1739 0.11751349817208734 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1709 0.11548623828412724 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1659 0.11210747180419373 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 6.757532959867012E-5 0.0 4 0.0 0.0 0.0 6.757532959867012E-5 0.0 5 6.757532959867012E-5 0.0 0.0 6.757532959867012E-5 0.0 6 6.757532959867012E-5 6.757532959867012E-5 0.0 6.757532959867012E-5 0.0 7 6.757532959867012E-5 6.757532959867012E-5 0.0 6.757532959867012E-5 0.0 8 6.757532959867012E-5 6.757532959867012E-5 0.0 6.757532959867012E-5 0.0 9 1.3515065919734025E-4 6.757532959867012E-5 0.0 6.757532959867012E-5 0.0 10-11 1.3515065919734025E-4 6.757532959867012E-5 0.0 6.757532959867012E-5 3.378766479933506E-5 12-13 1.3515065919734025E-4 6.757532959867012E-5 0.0 6.757532959867012E-5 6.757532959867012E-5 14-15 1.3515065919734025E-4 6.757532959867012E-5 0.0 1.3515065919734025E-4 6.757532959867012E-5 16-17 1.3515065919734025E-4 6.757532959867012E-5 0.0 2.703013183946805E-4 6.757532959867012E-5 18-19 1.3515065919734025E-4 6.757532959867012E-5 0.0 5.06814971990026E-4 6.757532959867012E-5 20-21 1.3515065919734025E-4 6.757532959867012E-5 0.0 8.446916199833765E-4 6.757532959867012E-5 22-23 1.3515065919734025E-4 6.757532959867012E-5 0.0 0.0016218079103680829 6.757532959867012E-5 24-25 1.3515065919734025E-4 6.757532959867012E-5 0.0 0.0035814924687295165 6.757532959867012E-5 26-27 1.3515065919734025E-4 6.757532959867012E-5 0.0 0.0058790536750843005 6.757532959867012E-5 28-29 1.3515065919734025E-4 6.757532959867012E-5 0.0 0.00902130650142246 6.757532959867012E-5 30-31 1.3515065919734025E-4 6.757532959867012E-5 0.0 0.015576113472493462 6.757532959867012E-5 32-33 1.3515065919734025E-4 6.757532959867012E-5 0.0 0.02713149483386605 6.757532959867012E-5 34-35 1.3515065919734025E-4 6.757532959867012E-5 0.0 0.042910334295155526 6.757532959867012E-5 36-37 1.3515065919734025E-4 6.757532959867012E-5 0.0 0.06703472696188076 6.757532959867012E-5 38-39 1.3515065919734025E-4 6.757532959867012E-5 0.0 0.10328889129156726 6.757532959867012E-5 40-41 1.3515065919734025E-4 6.757532959867012E-5 0.0 0.1450504449835454 6.757532959867012E-5 42-43 1.3515065919734025E-4 6.757532959867012E-5 0.0 0.18718366298831624 6.757532959867012E-5 44-45 1.3515065919734025E-4 6.757532959867012E-5 0.0 0.2336079144226026 6.757532959867012E-5 46-47 1.3515065919734025E-4 6.757532959867012E-5 0.0 0.29317556746383033 6.757532959867012E-5 48-49 1.3515065919734025E-4 6.757532959867012E-5 0.0 0.35980484244811906 6.757532959867012E-5 50-51 1.3515065919734025E-4 6.757532959867012E-5 0.0 0.4326848354202848 6.757532959867012E-5 52-53 1.3515065919734025E-4 6.757532959867012E-5 0.0 0.5002263773541555 6.757532959867012E-5 54-55 1.3515065919734025E-4 6.757532959867012E-5 0.0 0.5760458971638635 6.757532959867012E-5 56-57 1.3515065919734025E-4 6.757532959867012E-5 0.0 0.665042606245312 6.757532959867012E-5 58-59 1.3515065919734025E-4 6.757532959867012E-5 0.0 0.7640404641073637 6.757532959867012E-5 60-61 1.3515065919734025E-4 6.757532959867012E-5 0.0 0.8777359561571262 6.757532959867012E-5 62-63 1.3515065919734025E-4 6.757532959867012E-5 0.0 0.9948440023516214 6.757532959867012E-5 64-65 1.3515065919734025E-4 6.757532959867012E-5 0.0 1.1134387057972877 6.757532959867012E-5 66-67 1.3515065919734025E-4 6.757532959867012E-5 0.0 1.246190440793875 6.757532959867012E-5 68-69 1.3515065919734025E-4 6.757532959867012E-5 0.0 1.395937371184528 6.757532959867012E-5 70-71 1.3515065919734025E-4 6.757532959867012E-5 0.0 1.5737618510234284 6.757532959867012E-5 72-73 1.3515065919734025E-4 6.757532959867012E-5 0.0 1.7518904198455227 6.757532959867012E-5 74-75 1.3515065919734025E-4 6.757532959867012E-5 0.0 1.9341072961083368 6.757532959867012E-5 76-77 1.3515065919734025E-4 6.757532959867012E-5 0.0 2.1248724515653823 6.757532959867012E-5 78-79 1.3515065919734025E-4 6.757532959867012E-5 0.0 2.343951670124271 6.757532959867012E-5 80-81 1.3515065919734025E-4 6.757532959867012E-5 0.0 2.5917504037625942 6.757532959867012E-5 82-83 1.3515065919734025E-4 6.757532959867012E-5 0.0 2.8410357946520883 6.757532959867012E-5 84-85 1.3515065919734025E-4 6.757532959867012E-5 0.0 3.0937675273511145 6.757532959867012E-5 86-87 1.3515065919734025E-4 6.757532959867012E-5 0.0 3.3536622449876 6.757532959867012E-5 88-89 1.3515065919734025E-4 6.757532959867012E-5 0.0 3.639945128832366 6.757532959867012E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10600 0.0 29.013935 1 TACATGG 10880 0.0 27.660847 2 GTATCAA 19075 0.0 26.486036 1 ACATGGG 11140 0.0 25.730677 3 ATCGACG 155 1.266335E-6 24.822662 3 CATGGGA 7115 0.0 24.25988 4 GAGTACT 10955 0.0 23.728167 12-13 ATGGGAG 2445 0.0 23.39696 5 GGTATCA 14035 0.0 23.12831 1 TATCAAC 22400 0.0 22.552057 2 AGTACTT 11455 0.0 22.482729 12-13 TAAGGTG 475 0.0 22.453566 5 TCAACGC 23575 0.0 21.863966 4 ATCAACG 23240 0.0 21.853346 3 GTACTTT 12275 0.0 21.595015 14-15 CAACGCA 23670 0.0 21.54635 5 AACGCAG 24090 0.0 21.052551 6 ATGGGAT 2760 0.0 20.902304 5 CATGGGG 3815 0.0 20.372673 4 ACTTTTT 14295 0.0 20.325823 16-17 >>END_MODULE