FastQCFastQC Report
Fri 27 May 2016
SRR939392_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939392_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479830
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184451.2464269544474704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155881.0533642377840697No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA128050.8653020955109709No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122320.8265814316509329No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44040.2976017515525432No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39110.26428711406039884No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37150.2510423494590595No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36410.24604177506875788No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31900.21556530141975766No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24380.16474865356155774No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23610.15954535318246013No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23520.15893717521607212No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA22540.15231479291540245No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17700.11960833338964612No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17230.11643229289850861No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15810.10683659609549746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG120750.025.6261651
GTATCAA200300.025.1395571
TATAACG950.001233564624.976152
GGTATCA147900.024.6442851
TACATGG122450.024.492742
ACATGGG122000.023.0979563
TAGACCG1050.00222222822.5737975
GAGTACT124000.022.32319612-13
AGTACTT127650.021.88911412-13
CATGGGA78650.021.717484
GTATAGG5250.021.6957721
TAAGGTG4300.020.9463845
ATCAACG244050.020.663383
TAGTCGA1150.003767228720.6108595
TCAACGC245150.020.5734464
TATCAAC244300.020.570822
GTACTTT135550.020.43855514-15
CAACGCA248250.020.2986875
ATGGGAT31450.020.1979855
AACGCAG251700.020.0769586
Produced by FastQC (version 0.11.5)