FastQCFastQC Report
Fri 27 May 2016
SRR939391_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939391_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2829532
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185440.6553733974381629No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136170.4812456618267615No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102160.36104910635398363No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90280.31906336454226353No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT68160.24088789241471734No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT42730.15101437269484846No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA39340.1390335928344334No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT31460.11118446442733286No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA30980.10948807081877852No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30970.10945272928526698No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29770.10521174526388109No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28650.10125349351058763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG174550.027.646551
TACATGG174150.026.9235632
ACATGGG180750.024.9828723
GAGTACT162200.023.87784412-13
CATGGGA117850.022.599264
GTACTTT178150.022.15762714-15
GTATCAA358300.021.8212281
AGTACTT167900.021.75398812-13
ATGGGAG42800.020.2232995
CATGGGG69200.020.2127694
ACTTTTT211700.020.05596416-17
TACTTTT191250.019.30270214-15
TATCAAC412650.019.1112862
GGTATCA278700.018.5402581
ATCAACG427300.018.3647733
ATGGGAT45950.018.3107645
TCAACGC431800.018.2154354
CAACGCA430450.018.0412065
AACGCAG433150.017.8950796
AGTACAT133200.017.8022522
Produced by FastQC (version 0.11.5)