##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939391_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2829532 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.12112815829614 31.0 26.0 31.0 10.0 33.0 2 27.177378096448457 31.0 26.0 31.0 16.0 34.0 3 27.687998934099348 31.0 26.0 31.0 16.0 34.0 4 31.4256544898591 35.0 32.0 37.0 19.0 37.0 5 31.72271739637509 35.0 32.0 37.0 19.0 37.0 6 31.811928262341617 35.0 32.0 37.0 17.0 37.0 7 31.231278882868263 35.0 30.0 37.0 17.0 37.0 8 31.165357380655177 35.0 30.0 37.0 17.0 37.0 9 32.151060670103746 35.0 30.0 39.0 17.0 39.0 10-11 31.840227995300992 35.0 30.0 39.0 16.0 39.0 12-13 31.632870912928357 35.0 30.0 39.0 16.5 39.0 14-15 32.31672340160846 36.0 30.0 39.0 16.5 41.0 16-17 32.32691890390355 36.0 30.0 39.0 17.0 40.0 18-19 32.47889863058626 36.5 30.0 39.0 17.5 40.5 20-21 32.27278804410058 36.0 30.0 39.0 16.0 41.0 22-23 32.075016292446946 36.0 30.0 39.0 13.5 41.0 24-25 31.901105023728306 36.0 30.0 39.0 12.5 41.0 26-27 31.86054160193276 36.0 30.0 39.0 15.0 41.0 28-29 31.849622835154364 36.0 30.0 39.0 12.5 41.0 30-31 31.46293203257641 36.0 29.5 39.0 10.0 40.0 32-33 30.840045279572735 35.0 27.5 39.0 9.0 40.0 34-35 30.375307648049215 35.0 26.5 39.0 9.0 40.0 36-37 30.056465521506738 35.0 25.5 38.0 8.0 40.0 38-39 29.594592851397334 35.0 25.0 38.0 8.0 40.0 40-41 29.462154518839156 34.5 25.0 38.0 6.0 40.0 42-43 28.915580739146968 34.0 24.0 38.0 2.0 40.0 44-45 28.65218364733108 34.0 24.0 38.0 2.0 40.0 46-47 27.91907530998059 33.0 22.5 38.0 2.0 40.0 48-49 27.26768119250816 33.0 20.5 38.0 2.0 40.0 50-51 26.72210598784534 32.5 19.5 37.5 2.0 39.5 52-53 27.053162325077082 33.0 21.5 37.5 2.0 39.0 54-55 26.90544902831988 33.0 19.5 38.0 2.0 40.0 56-57 26.3738038657983 32.5 17.5 37.0 2.0 39.5 58-59 25.850411128059342 32.0 14.5 37.0 2.0 39.0 60-61 25.231035556410035 31.0 12.0 36.0 2.0 39.0 62-63 24.683184003573736 31.0 8.5 36.0 2.0 39.0 64-65 24.13114200510897 30.0 7.5 35.0 2.0 38.0 66-67 23.671179544885867 30.0 6.5 35.0 2.0 38.0 68-69 23.224676554285303 29.5 2.0 35.0 2.0 38.0 70-71 22.702238037951155 29.0 2.0 34.0 2.0 37.0 72-73 22.028949310345315 29.0 2.0 34.0 2.0 36.5 74-75 21.385426989339578 28.0 2.0 34.0 2.0 36.0 76-77 20.714944202786892 27.0 2.0 33.5 2.0 35.5 78-79 19.960101882572808 26.0 2.0 33.0 2.0 35.0 80-81 19.220538414126437 25.5 2.0 32.5 2.0 35.0 82-83 18.37856861134633 24.0 2.0 32.0 2.0 34.5 84-85 17.572637630533954 21.0 2.0 31.5 2.0 34.0 86-87 16.968395303534294 19.5 2.0 31.0 2.0 34.0 88-89 16.096342964136824 15.0 2.0 31.0 2.0 34.0 90-91 15.226858010441301 6.0 2.0 30.5 2.0 34.0 92-93 14.368480724020792 2.0 2.0 30.0 2.0 34.0 94-95 13.460999557524001 2.0 2.0 29.0 2.0 34.0 96-97 12.420607365458316 2.0 2.0 29.0 2.0 33.0 98-99 11.436869772103655 2.0 2.0 27.0 2.0 33.0 100-101 10.183857083079463 2.0 2.0 24.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.703160841120912 1101 2 3.4471262352954177 1101 3 3.041963315612218 1101 4 2.9630466746970683 1101 5 2.545988740877732 1101 6 2.2928189643157033 1101 7 2.7482796291722416 1101 8 2.722368889790868 1101 9 2.9398181043431393 1101 10-11 2.979394545063883 1101 12-13 2.977829908433801 1101 14-15 2.622234829941064 1101 16-17 2.40108829270779 1101 18-19 2.438138472714421 1101 20-21 2.5305976419454836 1101 22-23 2.711380003698874 1101 24-25 2.858007704654497 1101 26-27 2.9757589702755247 1101 28-29 2.5207631746039674 1101 30-31 2.5714428342786597 1101 32-33 2.8712815227847024 1101 34-35 2.926313186639973 1101 36-37 3.039623997565389 1101 38-39 2.923189856038224 1101 40-41 2.7904914076367398 1101 42-43 2.6461507766144905 1101 44-45 2.4045598743363428 1101 46-47 2.4528270586618106 1101 48-49 2.187967075149988 1101 50-51 2.3115904312975992 1101 52-53 2.0373222549628203 1101 54-55 2.176693570231354 1101 56-57 2.1730696446042153 1101 58-59 2.16235966960199 1101 60-61 1.952388102551133 1101 62-63 1.9158775505532404 1101 64-65 1.8279939927635596 1101 66-67 1.7301308758376415 1101 68-69 1.979778994450907 1101 70-71 1.7212997740791707 1101 72-73 1.4566637914468679 1101 74-75 1.3560351984406118 1101 76-77 1.2752297962448509 1101 78-79 1.155101670749719 1101 80-81 1.1917551312937285 1101 82-83 1.1768645113469418 1101 84-85 1.0977847675768828 1101 86-87 1.0441371794146228 1101 88-89 0.5096636377970469 1101 90-91 0.3336847110987069 1101 92-93 0.007308289634188725 1101 94-95 0.01954461125463247 1101 96-97 0.14656313502644913 1101 98-99 -0.1180183371528809 1101 100-101 -0.3239791219978301 1102 1 3.598555359463532 1102 2 3.5970234056528234 1102 3 3.0379730557272815 1102 4 2.8888760892211494 1102 5 2.4143743714641346 1102 6 2.116885776254346 1102 7 2.7546402720128107 1102 8 2.723510453578509 1102 9 3.118763962763765 1102 10-11 3.11052939402866 1102 12-13 3.1060340741702923 1102 14-15 3.0469379366496767 1102 16-17 2.654186681874691 1102 18-19 2.701214377431981 1102 20-21 2.968675736279195 1102 22-23 3.0786170461651317 1102 24-25 3.0597240893271405 1102 26-27 2.882798514852926 1102 28-29 2.855892926098445 1102 30-31 2.9534769075595655 1102 32-33 2.9528993275359134 1102 34-35 3.3564196513746367 1102 36-37 3.164661232015437 1102 38-39 2.9798508659221206 1102 40-41 2.8416056094541524 1102 42-43 3.139061962929862 1102 44-45 2.7867342147570078 1102 46-47 2.791404550110233 1102 48-49 2.7335790843878662 1102 50-51 2.453503185575695 1102 52-53 2.3085041845162273 1102 54-55 2.462612000609134 1102 56-57 2.4354674817244337 1102 58-59 2.506106136856637 1102 60-61 2.490888798289319 1102 62-63 2.4651049508060723 1102 64-65 2.3698136307338515 1102 66-67 2.218775164298002 1102 68-69 1.965885194017023 1102 70-71 1.7077467086494877 1102 72-73 1.7832947042610705 1102 74-75 1.6691397163376251 1102 76-77 1.3774731814638557 1102 78-79 1.2888997295315328 1102 80-81 1.3002832636356665 1102 82-83 1.1978878995205555 1102 84-85 1.31462832669202 1102 86-87 0.9223843691877214 1102 88-89 0.6435649213401575 1102 90-91 0.4035262224014069 1102 92-93 0.42643221049366886 1102 94-95 0.2277663509930168 1102 96-97 0.221608304777126 1102 98-99 -0.10246328208101652 1102 100-101 -0.3993651913855025 1103 1 3.710188131869323 1103 2 3.7613146964194435 1103 3 3.003389862689442 1103 4 2.975283554835677 1103 5 2.505527888802959 1103 6 2.5265609918287986 1103 7 2.974058363049817 1103 8 2.923407044843234 1103 9 3.0427589664065025 1103 10-11 3.014102656689815 1103 12-13 3.0986294404751114 1103 14-15 2.9562921865218144 1103 16-17 2.6782794117550495 1103 18-19 2.658274785350507 1103 20-21 2.8450238452372716 1103 22-23 3.028675896061525 1103 24-25 3.0154068096151008 1103 26-27 3.0054904242484675 1103 28-29 2.775171880756357 1103 30-31 2.848064932840746 1103 32-33 3.0313092607962595 1103 34-35 3.1143875520479725 1103 36-37 3.2953786621864474 1103 38-39 3.2196922881919114 1103 40-41 3.0279435598875075 1103 42-43 2.943515344821691 1103 44-45 2.7220504287887053 1103 46-47 2.59036186479932 1103 48-49 2.932825686975182 1103 50-51 2.7474976442923484 1103 52-53 2.5059978001877106 1103 54-55 2.6428643777166414 1103 56-57 2.649120435054165 1103 58-59 2.70352840325722 1103 60-61 2.719679034825404 1103 62-63 2.5962576857413033 1103 64-65 2.382904843726582 1103 66-67 2.325446670982572 1103 68-69 2.4630709794611754 1103 70-71 2.3791415455490856 1103 72-73 2.1871835787788036 1103 74-75 2.142411781179163 1103 76-77 2.17774738317895 1103 78-79 2.1223695770964106 1103 80-81 1.9537868732938755 1103 82-83 1.9075074457010288 1103 84-85 1.8880071291946976 1103 86-87 1.5893817374279848 1103 88-89 1.7747164370827946 1103 90-91 1.5031911352683611 1103 92-93 1.346866747696641 1103 94-95 1.2917390826374895 1103 96-97 1.1772246055729294 1103 98-99 0.641591192716259 1103 100-101 0.5726919745408843 1104 1 3.850445854562249 1104 2 3.7449194478344054 1104 3 3.184458082202916 1104 4 3.2322818096930774 1104 5 2.7502409680517417 1104 6 2.5044727044237014 1104 7 3.1010949869104216 1104 8 3.068036570292609 1104 9 3.4076840342939647 1104 10-11 3.432913319536084 1104 12-13 3.5083796861848633 1104 14-15 3.275997661459421 1104 16-17 3.0337619224634977 1104 18-19 3.0934287877356113 1104 20-21 3.327594992878666 1104 22-23 3.4320588574944644 1104 24-25 3.48717437401352 1104 26-27 3.3056865759468828 1104 28-29 3.109187868326483 1104 30-31 3.2217368830646542 1104 32-33 3.4297513964613238 1104 34-35 3.749403813414915 1104 36-37 3.630669542172729 1104 38-39 3.6293715360579206 1104 40-41 3.541847226268164 1104 42-43 3.625485783087999 1104 44-45 3.4537699143835816 1104 46-47 3.4385759127395623 1104 48-49 3.526992649769607 1104 50-51 3.282207683897184 1104 52-53 2.933017322150125 1104 54-55 2.8893365910850832 1104 56-57 3.2863841292639044 1104 58-59 3.5303535835761366 1104 60-61 3.578250653913287 1104 62-63 3.2300476349016485 1104 64-65 2.9629074506217705 1104 66-67 2.926086972708319 1104 68-69 2.9716105897043406 1104 70-71 2.784898539642896 1104 72-73 2.7133684677745826 1104 74-75 2.8101386996501247 1104 76-77 2.5830145181574515 1104 78-79 2.5393422540053514 1104 80-81 2.515552448712416 1104 82-83 2.6499938973359463 1104 84-85 2.5427958999468707 1104 86-87 2.275542556854411 1104 88-89 2.0113787249422757 1104 90-91 1.877138754775677 1104 92-93 1.7527244390985075 1104 94-95 1.4949342872818807 1104 96-97 1.4043532093506617 1104 98-99 1.035924451705574 1104 100-101 0.9920053437313356 1105 1 3.7734906576782734 1105 2 3.370500439268426 1105 3 1.9527873817656243 1105 4 2.6321921931839043 1105 5 2.354141958252505 1105 6 2.6045840056615823 1105 7 3.041348729067689 1105 8 2.8404546230861847 1105 9 3.24632509037108 1105 10-11 3.3956439510138807 1105 12-13 3.5528902639133157 1105 14-15 3.3700648841184275 1105 16-17 3.1446735032391118 1105 18-19 2.845207192417867 1105 20-21 3.3139794497584276 1105 22-23 3.3923418894511777 1105 24-25 3.146165674429234 1105 26-27 3.3206301802365523 1105 28-29 3.2885609124615875 1105 30-31 3.3554443550466466 1105 32-33 3.7666241708219665 1105 34-35 4.042897161547302 1105 36-37 3.9327890959400698 1105 38-39 4.070072281663169 1105 40-41 3.770751839154652 1105 42-43 3.8078141166011825 1105 44-45 3.6835038910647917 1105 46-47 3.767041486948095 1105 48-49 3.9903480275309384 1105 50-51 3.6099499028307243 1105 52-53 3.3355781241767737 1105 54-55 3.6271583549476354 1105 56-57 3.6763421472180404 1105 58-59 3.6297686611427196 1105 60-61 3.764498168134253 1105 62-63 3.706271049270093 1105 64-65 3.625508487583442 1105 66-67 3.609713615120981 1105 68-69 3.725770820322971 1105 70-71 3.7336816769401153 1105 72-73 3.6067607404060915 1105 74-75 3.6296840911314234 1105 76-77 3.5188368678159954 1105 78-79 3.515984666662657 1105 80-81 3.370340817065042 1105 82-83 3.252882950658794 1105 84-85 3.2055169223150273 1105 86-87 2.884255038933677 1105 88-89 3.086553667261928 1105 90-91 3.032380652549138 1105 92-93 2.972844835589896 1105 94-95 2.583395956183823 1105 96-97 2.529007769566892 1105 98-99 2.2461305080139287 1105 100-101 1.9603408095676116 1106 1 3.2776794293838236 1106 2 2.50504395689919 1106 3 2.5585549326338466 1106 4 1.1609276784294593 1106 5 1.7059306021216898 1106 6 2.112759141487725 1106 7 2.647254769336147 1106 8 2.728346388293385 1106 9 3.0090586580710905 1106 10-11 2.8120794469124633 1106 12-13 3.262395963284785 1106 14-15 2.9623478286161316 1106 16-17 1.6592713513268933 1106 18-19 2.7258996394190405 1106 20-21 2.113157035781647 1106 22-23 2.976841729655714 1106 24-25 3.135712920620776 1106 26-27 2.960043198240175 1106 28-29 2.3861160418959066 1106 30-31 1.9597045020584751 1106 32-33 1.9235113552259229 1106 34-35 2.485396362569986 1106 36-37 2.939630472311645 1106 38-39 1.4875166195773026 1106 40-41 1.3140521480925464 1106 42-43 2.795971142274986 1106 44-45 2.9031225125626072 1106 46-47 3.0232788364740344 1106 48-49 3.1517734030502744 1106 50-51 2.9673452368032436 1106 52-53 2.5761608449608353 1106 54-55 2.6426073801953187 1106 56-57 2.928184056241154 1106 58-59 3.1786479623038986 1106 60-61 3.077285000988198 1106 62-63 2.769410497443097 1106 64-65 2.7361615183707 1106 66-67 2.902913785569474 1106 68-69 3.145259474845183 1106 70-71 2.77305101751751 1106 72-73 2.8812371439429008 1106 74-75 2.740681214813108 1106 76-77 2.629172438285792 1106 78-79 2.5791624657464958 1106 80-81 2.6214540587823087 1106 82-83 2.509287552768093 1106 84-85 2.5244526845393835 1106 86-87 2.158665393679602 1106 88-89 2.2952071241704175 1106 90-91 2.3920205801085306 1106 92-93 2.1394827390734363 1106 94-95 2.3699987046657647 1106 96-97 2.3653079719744756 1106 98-99 1.957873343850725 1106 100-101 1.5657248513274915 1107 1 2.599319653348335 1107 2 2.9244152511657227 1107 3 -0.6687635709757274 1107 4 1.533381407976755 1107 5 0.44840738720492723 1107 6 1.3986831360695042 1107 7 2.2144942428684082 1107 8 1.2828544706887577 1107 9 -1.767796075881126 1107 10-11 -1.0983586953468532 1107 12-13 1.4740308247976337 1107 14-15 1.3303072784055772 1107 16-17 -0.9972980355115411 1107 18-19 0.21649067737634908 1107 20-21 0.3979960487215095 1107 22-23 0.44188083574652737 1107 24-25 -0.0416746583737968 1107 26-27 0.5270303058614623 1107 28-29 0.7309600907511289 1107 30-31 1.5494484944902958 1107 32-33 -0.10337258558413609 1107 34-35 1.0654148082426929 1107 36-37 1.951003368782949 1107 38-39 -0.10401166048759336 1107 40-41 0.7757965029278289 1107 42-43 0.2706472156749591 1107 44-45 0.9671485579271035 1107 46-47 0.8746186252745041 1107 48-49 0.6741007112187525 1107 50-51 1.4826068696070571 1107 52-53 1.5769244215885898 1107 54-55 1.2998222722823307 1107 56-57 1.0025765406402343 1107 58-59 0.9986224883260988 1107 60-61 1.5630797908579481 1107 62-63 1.7095724615205832 1107 64-65 1.7601660309986897 1107 66-67 1.023354677990067 1107 68-69 0.4834490249165171 1107 70-71 0.3043148985496593 1107 72-73 0.8689752912713082 1107 74-75 0.6395580543397976 1107 76-77 0.6903161010921792 1107 78-79 1.345484123489392 1107 80-81 1.6380938302608072 1107 82-83 1.5257530678915465 1107 84-85 1.7443883060939704 1107 86-87 1.4172734347448106 1107 88-89 1.4525351118453855 1107 90-91 1.4909938359563544 1107 92-93 1.4355739337163342 1107 94-95 1.413929913522841 1107 96-97 1.4693794442395731 1107 98-99 1.3359920117028743 1107 100-101 1.1178485967672565 1108 1 2.9381906189636027 1108 2 3.0681388442239026 1108 3 2.512005345373833 1108 4 2.731905704604735 1108 5 2.14548535740078 1108 6 2.08007761774056 1108 7 2.069362666349921 1108 8 2.3290647663304718 1108 9 2.8183034101542788 1108 10-11 -0.6096212444952123 1108 12-13 1.9897582468788606 1108 14-15 1.4067354222523747 1108 16-17 1.4626719546522011 1108 18-19 0.21329855904581763 1108 20-21 -0.11437517664497676 1108 22-23 0.9127600079180773 1108 24-25 -0.438580744463529 1108 26-27 0.7449081515876976 1108 28-29 0.24441457099817043 1108 30-31 0.16892867560413904 1108 32-33 1.2867867724479787 1108 34-35 -0.9139204923435287 1108 36-37 0.6007643185494338 1108 38-39 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2312 50-51 -0.20239441951359538 2312 52-53 1.0780464745398604 2312 54-55 -0.9189787827684448 2312 56-57 -3.079385633772901 2312 58-59 -0.4635100484517807 2312 60-61 0.9459704385538927 2312 62-63 -3.947506342928353 2312 64-65 -1.5169887812296174 2312 66-67 1.72807426032373 2312 68-69 -0.0036347448720697173 2312 70-71 -0.9340016339010901 2312 72-73 -0.790962413632851 2312 74-75 -0.6805279775017006 2312 76-77 -1.234864051148083 2312 78-79 0.6903725594716015 2312 80-81 0.9797553791111859 2312 82-83 -0.34572189478815574 2312 84-85 -0.6791916439724872 2312 86-87 0.32173784852174947 2312 88-89 -1.7790147409380594 2312 90-91 -1.604929288971329 2312 92-93 -1.1896325116242927 2312 94-95 -1.069902675009546 2312 96-97 -0.9421669621341557 2312 98-99 -0.20783196226433098 2312 100-101 -0.03752436619230082 2313 1 -1.9109170861514357 2313 2 -0.7710336464275116 2313 3 -2.1388100124907297 2313 4 -2.1357700051829163 2313 5 -1.6152148634742574 2313 6 -3.2521019288278232 2313 7 0.5229782363619364 2313 8 -2.9821180900067574 2313 9 -3.877304600426239 2313 10-11 -4.875788290476159 2313 12-13 -3.4963105802008236 2313 14-15 -3.3198818999902144 2313 16-17 -1.6455999163406645 2313 18-19 -4.352988054852524 2313 20-21 -6.338556562719781 2313 22-23 -5.349937218723888 2313 24-25 -1.699415571556635 2313 26-27 -5.085683243013289 2313 28-29 -5.9910289581687515 2313 30-31 -5.3535537181710815 2313 32-33 -4.730609520342366 2313 34-35 0.3016117225161956 2313 36-37 0.5381143551381591 2313 38-39 -2.4365934674881338 2313 40-41 -5.940696917612023 2313 42-43 -3.0217197855916815 2313 44-45 -1.5182696661422916 2313 46-47 -3.7075341621940296 2313 48-49 -3.138209638309963 2313 50-51 -2.2460699155012307 2313 52-53 -4.254778789301529 2313 54-55 -3.7247739324181772 2313 56-57 -2.9946753773254997 2313 58-59 -0.9414387890820706 2313 60-61 0.9607326850624105 2313 62-63 0.7823908767772032 2313 64-65 1.251955868077065 2313 66-67 0.8895824104233014 2313 68-69 -1.7558118936874898 2313 70-71 -0.34918188852460474 2313 72-73 -2.0301765932942466 2313 74-75 -2.204923671048597 2313 76-77 -1.0957433689029692 2313 78-79 -0.5736015339062028 2313 80-81 -0.8858845247094287 2313 82-83 -0.7096604019423616 2313 84-85 -0.9574613325707446 2313 86-87 -0.815026546532879 2313 88-89 -1.3386220369311168 2313 90-91 -3.1129418541028464 2313 92-93 -3.530370138260917 2313 94-95 -2.922711943623499 2313 96-97 -2.4561330690421546 2313 98-99 -1.9649888607354988 2313 100-101 -1.6425144982587945 2314 1 -2.2480278537191083 2314 2 -0.7762721899268143 2314 3 -2.5794739355024205 2314 4 -1.5159271855680458 2314 5 -2.520995728944399 2314 6 -2.7795369967097585 2314 7 -0.9435798103157609 2314 8 0.7157025454942705 2314 9 -1.4514796053070995 2314 10-11 -2.3305826293445904 2314 12-13 -1.3784816944707536 2314 14-15 -3.87628047681644 2314 16-17 -1.9307775852267817 2314 18-19 -1.2046994494672383 2314 20-21 -1.4115293199226144 2314 22-23 -2.056366692231837 2314 24-25 -2.46255999798419 2314 26-27 -2.261657627663638 2314 28-29 -1.8705911736756953 2314 30-31 -1.5013069107355754 2314 32-33 -8.339522083103567 2314 34-35 -8.737942420584343 2314 36-37 -5.113782289904535 2314 38-39 -3.369023832332008 2314 40-41 -2.622768210408463 2314 42-43 -1.800555854985479 2314 44-45 -1.3513094995224897 2314 46-47 -2.580496379701483 2314 48-49 -2.2343074874341333 2314 50-51 -1.6294572624580006 2314 52-53 -1.0437730689698448 2314 54-55 -0.09851106759930772 2314 56-57 -0.21255144162171646 2314 58-59 -2.884869335823673 2314 60-61 -3.7857184568737594 2314 62-63 -3.1365192261043013 2314 64-65 -3.6765082186055373 2314 66-67 -3.354558426580404 2314 68-69 -4.785665441934288 2314 70-71 -6.614372757036255 2314 72-73 -6.341303307106138 2314 74-75 -6.27817529488409 2314 76-77 -7.464071547232393 2314 78-79 -6.61144061480031 2314 80-81 -5.6458975131705955 2314 82-83 -4.78649857853431 2314 84-85 -4.770343489144306 2314 86-87 -4.6815073144599 2314 88-89 -4.9059069706495855 2314 90-91 -5.1437910238378795 2314 92-93 -5.337712749489896 2314 94-95 -4.700398185968329 2314 96-97 -4.825925042499164 2314 98-99 -4.725842616812493 2314 100-101 -4.605849880256995 2315 1 -24.77920775502014 2315 2 -12.942478864336499 2315 3 -8.30797269452573 2315 4 -5.731321622802604 2315 5 -2.3419801079192126 2315 6 -2.3726732885172623 2315 7 -7.587447790533197 2315 8 -1.700677767639057 2315 9 -4.866196149836355 2315 10-11 -3.318906733874435 2315 12-13 -3.722699023771302 2315 14-15 -3.3260554481588116 2315 16-17 -5.698585745511696 2315 18-19 -6.886230994243753 2315 20-21 -5.677002880280504 2315 22-23 -7.179100868386815 2315 24-25 -23.86533177989841 2315 26-27 -10.575898720631972 2315 28-29 -7.106506945863476 2315 30-31 -5.419067868909469 2315 32-33 -6.070565501471979 2315 34-35 -6.924500578084803 2315 36-37 -5.383036474274778 2315 38-39 -4.088430894516275 2315 40-41 -4.107685921914687 2315 42-43 -3.616226488055112 2315 44-45 -8.36252974648821 2315 46-47 -4.75811732202402 2315 48-49 -4.466700253549117 2315 50-51 -6.39108003049315 2315 52-53 -9.419307517600625 2315 54-55 -5.46866516611918 2315 56-57 -12.322744090388557 2315 58-59 -7.6570420763929405 2315 60-61 -4.863710088356029 2315 62-63 -5.0098838032700606 2315 64-65 -5.1112965753884545 2315 66-67 -8.040806395597642 2315 68-69 -5.361537544929785 2315 70-71 -5.456521346341358 2315 72-73 -5.664401757032472 2315 74-75 -5.147006219550946 2315 76-77 -5.890579597876538 2315 78-79 -5.379100530607246 2315 80-81 -4.602975334950216 2315 82-83 -3.990486321578402 2315 84-85 -3.8474645880646943 2315 86-87 -3.561513155136403 2315 88-89 -4.856005718624171 2315 90-91 -4.699793020627512 2315 92-93 -4.825406453008959 2315 94-95 -5.1935563301924965 2315 96-97 -5.411057803169982 2315 98-99 -5.11246109678423 2315 100-101 -4.508999680021141 2316 1 -1.72106212903795 2316 2 -0.19964465456446234 2316 3 -4.236284100852217 2316 4 -0.3227843174133511 2316 5 -4.477317710650851 2316 6 -0.7976141035454347 2316 7 -3.9623161166505874 2316 8 -3.6282588228299844 2316 9 -2.728871929592831 2316 10-11 -2.1808210809566475 2316 12-13 -4.170954764232395 2316 14-15 -3.5059014241789512 2316 16-17 -1.5263274363369987 2316 18-19 -5.5126677026073665 2316 20-21 -2.3252060292175187 2316 22-23 -5.513308039457005 2316 24-25 -2.281120447283289 2316 26-27 -2.131958892410143 2316 28-29 -5.0084095781967015 2316 30-31 -8.530735644454463 2316 32-33 -5.835314016710779 2316 34-35 -2.7624664045182072 2316 36-37 -4.692166045536812 2316 38-39 -4.57885843083486 2316 40-41 -2.830997934266154 2316 42-43 -2.424140368003904 2316 44-45 -0.13565714230263382 2316 46-47 -1.6587502930081683 2316 48-49 -1.702158645122406 2316 50-51 0.38096913503906293 2316 52-53 1.348976230057854 2316 54-55 1.7446080294219328 2316 56-57 -0.10867252846190922 2316 58-59 -2.645988362812311 2316 60-61 -5.570316618964451 2316 62-63 -1.7924294255068638 2316 64-65 -3.16849818091249 2316 66-67 -2.4754255336064332 2316 68-69 0.4644093495953392 2316 70-71 1.4138097253657023 2316 72-73 1.982644482413459 2316 74-75 2.0177384202491346 2316 76-77 1.1778914713231892 2316 78-79 1.1433891084463532 2316 80-81 1.995750946194395 2316 82-83 1.6432990248936221 2316 84-85 1.0638433959112774 2316 86-87 0.4046179813867177 2316 88-89 -1.3028812566989263 2316 90-91 -0.9718263202696722 2316 92-93 -1.3485947114180092 2316 94-95 -2.5959554868554147 2316 96-97 -4.070979342442222 2316 98-99 -2.006612721633438 2316 100-101 -1.3162534349051036 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41392.0 3 13913.0 4 14904.0 5 17335.0 6 19015.0 7 24226.0 8 30406.0 9 35898.0 10 40752.0 11 44963.0 12 50398.0 13 54601.0 14 58496.0 15 62839.0 16 66789.0 17 68647.0 18 65577.0 19 65584.0 20 69366.0 21 73910.0 22 79475.0 23 85749.0 24 91965.0 25 98383.0 26 105554.0 27 112835.0 28 120119.0 29 127048.0 30 135501.0 31 143174.0 32 150863.0 33 155990.0 34 159585.0 35 150000.0 36 121915.0 37 63451.0 38 8886.0 39 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.36063637575887 18.29118718925248 14.281490914046485 21.066685520942166 2 16.38537457286838 20.839031480196876 38.67909431580029 24.096499631134456 3 20.599941395920585 25.566422721230396 29.109503866439873 24.724132016409143 4 12.9200422357243 16.496411783324596 35.813439139127745 34.770106841823356 5 13.688323536676428 37.98355675041297 32.25715260654846 16.07096710636214 6 31.51164634077295 35.03668852124877 17.233095254271834 16.218569883706447 7 28.80004031481552 33.98081806679661 19.394917813569176 17.8242238048187 8 27.759924274346364 32.029589520859695 18.9889283699682 21.221557834825738 9 27.914899822079736 16.95760245457052 19.932590624483485 35.19490709886626 10-11 26.008382197944215 26.17057681756087 25.3936531624112 22.427387822083716 12-13 28.069221915461846 24.032221303703285 26.369645085590065 21.5289116952448 14-15 24.36646691503986 24.92813495259753 26.454110965325473 24.251287167037137 16-17 22.227930120461036 28.49068994215046 26.70852209655605 22.572857840832455 18-19 21.924168148441936 26.379493398334997 30.295875434953473 21.400463018269594 20-21 23.454522025205144 24.78699817080575 30.25263201121802 21.50584779277109 22-23 22.744652837847568 24.991941140544196 30.265093359197547 21.998312662410687 24-25 22.743539650995185 25.38097747943834 30.383627157579056 21.491855711987423 26-27 22.558242918220813 25.223637928652646 30.860812088881335 21.357307064245205 28-29 21.739153538777703 26.030143027669045 30.76519900360479 21.465504429948467 30-31 21.81680983950522 25.72802449817489 30.486535000074376 21.968630662245513 32-33 21.85650231205047 25.9275213890795 30.18238151294858 22.033594785921448 34-35 22.734881575179244 25.60903265468343 30.085224436095377 21.57086133404195 36-37 22.671678545066587 25.33398197106119 30.28253844197572 21.711801041896504 38-39 22.464394507264636 25.59526784084606 30.186015108743614 21.754322543145687 40-41 22.184604213882356 25.872216382059893 30.304867104463266 21.63831229959448 42-43 22.148989102569438 26.121268025367307 29.38265247486724 22.347090397196016 44-45 22.17990848762416 26.0340459459536 29.323046333981935 22.4629992324403 46-47 22.622562618107068 26.380295415759015 28.69585359301378 22.301288373120137 48-49 22.865809689460608 26.396852869851724 28.35822541095624 22.37911202973143 50-51 22.336358869710626 26.829298103902726 28.579503585296035 22.25483944109061 52-53 22.615261081746883 27.138851917840647 27.823182477282295 22.422704523130175 54-55 21.895709918642012 26.650767498773533 28.311672843899853 23.141849738684602 56-57 22.465883694655084 27.102850079513217 27.77577273033155 22.655493495500153 58-59 22.903797382735803 26.46623296438361 27.663190355356466 22.966779297524123 60-61 22.53543743421734 26.513246824064083 28.580864980565067 22.370450761153506 62-63 22.564825632855374 26.579270667374754 28.208036820676224 22.647866879093645 64-65 22.222424802963186 26.994694056369568 28.16817938426272 22.614701756404525 66-67 22.27218054537092 26.777157631266146 27.96274966424631 22.98791215911662 68-69 22.216837878755953 26.81050122847426 28.090320522274624 22.882340370495157 70-71 22.402975676988827 26.945692159720775 27.73842811955969 22.91290404373071 72-73 22.35789701220949 26.59881253798301 27.942458942907212 23.100831506900285 74-75 21.97985667782906 27.63466697557203 27.519252562373552 22.86622378422535 76-77 22.384974922603416 26.854661976318834 27.517175674261118 23.243187426816633 78-79 22.192737736890315 27.032682025912973 27.698712994245415 23.075867242951293 80-81 22.14628727216563 27.58209334850458 27.465634436271557 22.805984943058235 82-83 22.26825575353694 27.167511172436665 27.33897510309714 23.225257970929256 84-85 22.05083786149673 27.143903772417747 27.12686726417231 23.678391101913206 86-87 22.35356154928465 26.986796537486423 27.32606422216863 23.3335776910603 88-89 22.192121724453365 26.99149941795998 26.923343895166923 23.89303496241973 90-91 22.025666269776544 27.000819460332153 27.22150878816747 23.752005481723838 92-93 22.240076160011107 27.129011247980806 27.248701890435694 23.382210701572394 94-95 21.99907055559806 27.018052615620903 27.173709758316118 23.80916707046492 96-97 21.787875164414682 26.862609111562836 27.129640701325503 24.219875022696975 98-99 22.08314220904771 27.047324233425517 26.461398740980396 24.408134816546376 100-101 22.259543045634455 26.656835383260514 26.859488163460952 24.22413340764408 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9854.0 1 6748.5 2 3785.0 3 3983.0 4 3698.0 5 3641.0 6 5043.5 7 8575.5 8 12618.5 9 13893.5 10 11866.5 11 9516.0 12 8445.0 13 7939.5 14 7759.0 15 7500.5 16 7313.0 17 7369.5 18 7502.0 19 7738.5 20 8171.5 21 8965.0 22 9946.5 23 11009.0 24 12369.5 25 14066.5 26 16127.0 27 18677.5 28 21459.0 29 24497.0 30 27505.5 31 30915.5 32 34825.0 33 37706.0 34 40382.0 35 43305.0 36 46288.0 37 49084.0 38 51688.5 39 54138.5 40 57129.5 41 62388.5 42 68128.0 43 72714.5 44 77360.5 45 83074.5 46 89710.0 47 101852.5 48 123197.5 49 127749.5 50 114170.0 51 105856.5 52 102673.0 53 102629.0 54 101281.0 55 97373.0 56 91710.0 57 84788.0 58 78049.5 59 70684.0 60 62097.0 61 53016.5 62 43552.0 63 34265.0 64 26581.0 65 20860.5 66 16235.0 67 12565.5 68 9372.5 69 6718.0 70 4875.5 71 3676.5 72 2971.0 73 2374.0 74 1871.0 75 1480.5 76 1096.0 77 776.0 78 534.5 79 338.0 80 238.5 81 174.0 82 110.5 83 73.0 84 57.5 85 34.0 86 17.0 87 13.0 88 12.5 89 12.0 90 8.0 91 3.5 92 2.0 93 2.5 94 2.0 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.527933241256858 2 3.709518040439196 3 1.0988389599410786 4 0.960052757841226 5 0.9858874188381683 6 0.12652268997134508 7 1.816448797893079 8 0.9465522920398144 9 0.14521836119895445 10-11 1.0317077170358913 12-13 0.8371172335213032 14-15 0.661169408934057 16-17 0.14180790321509 18-19 0.15456619681275913 20-21 0.16932128705383082 22-23 0.6152784276693106 24-25 1.398482151818746 26-27 0.22767015888139805 28-29 0.2387850711707802 30-31 0.2169263326938872 32-33 0.23724771446302784 34-35 0.7175214841182217 36-37 0.2910198577008494 38-39 0.28227282815674115 40-41 0.2685073008539928 42-43 0.21121867503177205 44-45 0.17656630142369834 46-47 0.21514158525155397 48-49 0.6300511886771382 50-51 0.24357384896159504 52-53 0.26520286747066296 54-55 0.2975757121672418 56-57 0.21565403748747144 58-59 0.2016411194501423 60-61 0.18958965652270413 62-63 0.17783859663011412 64-65 0.1720955974344874 66-67 0.18692137074258217 68-69 0.19439610508027474 70-71 0.201588107149875 72-73 0.2667225534116596 74-75 0.23540995472042725 76-77 0.23224688747114364 78-79 0.22583239913879752 80-81 0.23495051478477713 82-83 0.24995299576042965 84-85 0.21853437246866264 86-87 0.2050869189675183 88-89 0.20650058030798027 90-91 0.22380026096188343 92-93 0.21215522566982808 94-95 0.22406532246322008 96-97 0.24807989448431758 98-99 0.26207514175489094 100-101 0.3160946757272934 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2829532.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.8638495723822 #Duplication Level Percentage of deduplicated Percentage of total 1 74.47441964871744 37.13581258354112 2 12.159742905938852 12.126631822011532 3 5.031198859294157 7.526248292657545 4 2.649136848952007 5.283846453311898 5 1.5864342131559541 3.9552858480644506 6 1.0169421686747968 3.0425190793567323 7 0.6843937614136838 2.3888555297186134 8 0.46757895709964015 1.8652229424022246 9 0.33358486951144223 1.4970443177647172 >10 1.465763577628388 12.29716894732582 >50 0.07660474763860453 2.6966460212450154 >100 0.047966307439332195 4.501375982254734 >500 0.0036294201094066924 1.1898441581947106 >1k 0.002209212240505646 2.2029211371136945 >5k 1.5780087432183185E-4 0.6233472486994692 >10k+ 2.3670131148274777E-4 1.6672296363378822 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18544 0.6553733974381629 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13617 0.4812456618267615 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10216 0.36104910635398363 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9028 0.31906336454226353 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6816 0.24088789241471734 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4273 0.15101437269484846 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3934 0.1390335928344334 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3146 0.11118446442733286 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3098 0.10948807081877852 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3097 0.10945272928526698 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2977 0.10521174526388109 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2865 0.10125349351058763 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 3.53415335115489E-5 8 0.0 0.0 0.0 0.0 3.53415335115489E-5 9 0.0 0.0 0.0 0.0 3.53415335115489E-5 10-11 0.0 0.0 0.0 0.0 3.53415335115489E-5 12-13 0.0 0.0 0.0 7.06830670230978E-5 3.53415335115489E-5 14-15 0.0 0.0 0.0 7.06830670230978E-5 2.1204920106929345E-4 16-17 0.0 0.0 0.0 1.2369536729042117E-4 2.1204920106929345E-4 18-19 0.0 0.0 0.0 2.297199678250679E-4 2.1204920106929345E-4 20-21 0.0 0.0 0.0 5.654645361847825E-4 2.1204920106929345E-4 22-23 0.0 0.0 0.0 0.001166270605881114 2.1204920106929345E-4 24-25 0.0 0.0 0.0 0.0021204920106929342 2.1204920106929345E-4 26-27 0.0 0.0 0.0 0.0034811410508875673 3.8875686862703795E-4 28-29 0.0 0.0 0.0 0.006361476032078803 3.8875686862703795E-4 30-31 0.0 0.0 0.0 0.014065930337596465 3.8875686862703795E-4 32-33 0.0 0.0 0.0 0.024986464192665078 3.8875686862703795E-4 34-35 0.0 0.0 0.0 0.04140260650877954 3.8875686862703795E-4 36-37 0.0 0.0 0.0 0.0668131691035832 4.0642763538281245E-4 38-39 0.0 0.0 0.0 0.10088240740871636 4.240984021385869E-4 40-41 0.0 0.0 0.0 0.140005485006001 4.240984021385869E-4 42-43 0.0 0.0 0.0 0.17905787953626254 4.240984021385869E-4 44-45 0.0 0.0 0.0 0.22885410025403494 4.240984021385869E-4 46-47 0.0 0.0 0.0 0.2892174394917605 4.240984021385869E-4 48-49 0.0 0.0 0.0 0.3550410456570203 4.240984021385869E-4 50-51 0.0 0.0 0.0 0.42823336155943814 4.240984021385869E-4 52-53 0.0 0.0 0.0 0.5005421391240672 4.594399356501358E-4 54-55 0.0 0.0 0.0 0.5742999195626697 4.594399356501358E-4 56-57 0.0 0.0 0.0 0.6663469435934988 4.947814691616847E-4 58-59 0.0 0.0 0.0 0.769915307549093 4.947814691616847E-4 60-61 0.0 3.53415335115489E-5 0.0 0.8895640692524418 4.947814691616847E-4 62-63 0.0 3.53415335115489E-5 0.0 1.0066328990094475 5.301230026732336E-4 64-65 0.0 3.53415335115489E-5 0.0 1.124108156401836 5.301230026732336E-4 66-67 0.0 3.53415335115489E-5 0.0 1.2622758816652366 5.301230026732336E-4 68-69 0.0 3.53415335115489E-5 0.0 1.4233625914108763 5.47793769429008E-4 70-71 0.0 3.53415335115489E-5 0.0 1.6036220830865315 5.654645361847825E-4 72-73 0.0 3.53415335115489E-5 0.0 1.7835281594270713 5.654645361847825E-4 74-75 0.0 3.53415335115489E-5 0.0 1.9705908963036998 5.654645361847825E-4 76-77 0.0 3.53415335115489E-5 1.767076675577445E-5 2.1682560932337926 5.654645361847825E-4 78-79 0.0 3.53415335115489E-5 3.53415335115489E-5 2.3927455140991514 5.654645361847825E-4 80-81 0.0 3.53415335115489E-5 3.53415335115489E-5 2.645578844840772 5.654645361847825E-4 82-83 0.0 3.53415335115489E-5 3.53415335115489E-5 2.896768794274106 5.654645361847825E-4 84-85 0.0 3.53415335115489E-5 3.53415335115489E-5 3.1597451451335417 6.538183699636548E-4 86-87 0.0 3.53415335115489E-5 3.53415335115489E-5 3.431503867070597 7.068306702309781E-4 88-89 0.0 3.53415335115489E-5 3.53415335115489E-5 3.720438574294265 7.068306702309781E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 17455 0.0 27.64655 1 TACATGG 17415 0.0 26.923563 2 ACATGGG 18075 0.0 24.982872 3 GAGTACT 16220 0.0 23.877844 12-13 CATGGGA 11785 0.0 22.59926 4 GTACTTT 17815 0.0 22.157627 14-15 GTATCAA 35830 0.0 21.821228 1 AGTACTT 16790 0.0 21.753988 12-13 ATGGGAG 4280 0.0 20.223299 5 CATGGGG 6920 0.0 20.212769 4 ACTTTTT 21170 0.0 20.055964 16-17 TACTTTT 19125 0.0 19.302702 14-15 TATCAAC 41265 0.0 19.111286 2 GGTATCA 27870 0.0 18.540258 1 ATCAACG 42730 0.0 18.364773 3 ATGGGAT 4595 0.0 18.310764 5 TCAACGC 43180 0.0 18.215435 4 CAACGCA 43045 0.0 18.041206 5 AACGCAG 43315 0.0 17.895079 6 AGTACAT 13320 0.0 17.802252 2 >>END_MODULE