FastQCFastQC Report
Fri 27 May 2016
SRR939391_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939391_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2829532
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262750.9285987930159475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215770.7625642685786908No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166250.5875529946295006No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA136320.4817757848294347No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT103660.36635033638071596No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT64020.22625649754093607No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56510.19971500587376284No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA51770.1829631189892887No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49360.1744458094130054No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT48800.17246668353635866No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA46030.1626770787536596No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42790.15122642189591778No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC41120.1453243857994891No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31190.11023024302252103No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT30490.10775633567671261No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT30370.10733223727457403No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT29990.10598925900113516No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC28630.10118281044356453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG192950.025.1565971
TACATGG192150.024.6622922
ACATGGG198100.022.6732253
AGTACTT191300.022.29114212-13
GAGTACT189200.022.25056612-13
GTACTTT200650.021.07530414-15
CATGGGA129500.020.7810754
GTATCAA394850.020.5766831
TACTTTT211800.020.14478114-15
ACTTTTT228600.018.95449816-17
TAAGGTG10750.018.9517565
GTAAGGT8550.018.8409254
ATGGGAG42250.018.8396135
CATGGGG71700.018.634574
GGTATCA308450.018.579681
ACGCATA3650.018.49649664-65
ATTCGCG5400.018.42547880-81
ATGGGAT50900.018.0581555
TATCAAC460650.017.5707652
CAATTCG5800.017.56444578-79
Produced by FastQC (version 0.11.5)