##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939391_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2829532 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.987017287664532 31.0 31.0 31.0 30.0 34.0 2 31.225957154752095 31.0 31.0 34.0 30.0 34.0 3 31.209219051065688 31.0 31.0 34.0 28.0 34.0 4 35.14696812052311 35.0 35.0 37.0 33.0 37.0 5 34.772759240750766 35.0 35.0 37.0 32.0 37.0 6 34.7384853749666 35.0 35.0 37.0 32.0 37.0 7 34.60832533436625 35.0 35.0 37.0 30.0 37.0 8 34.63605359472874 35.0 35.0 37.0 30.0 37.0 9 35.95604326086434 37.0 35.0 39.0 30.0 39.0 10-11 35.80689103357021 37.0 35.0 39.0 30.0 39.0 12-13 35.75918385089831 37.0 35.0 39.0 30.0 39.0 14-15 36.55607217023875 38.0 35.0 40.0 30.0 41.0 16-17 36.590297794829674 38.0 35.0 40.0 30.5 41.0 18-19 36.588581256547016 38.0 35.0 40.0 30.0 41.0 20-21 36.4912937899271 38.0 35.0 40.0 30.0 41.0 22-23 36.342802802724975 38.0 35.0 40.0 30.0 41.0 24-25 36.08761908329717 38.0 34.0 40.0 29.5 41.0 26-27 35.67095954383976 38.0 34.0 40.0 27.5 41.0 28-29 35.375083229311414 38.0 34.0 40.0 27.0 41.0 30-31 35.111136046526426 37.5 33.5 40.0 27.0 41.0 32-33 34.83131698104138 37.0 33.0 40.0 26.0 41.0 34-35 34.70509999533492 37.0 33.0 40.0 25.5 41.0 36-37 34.62957990932776 37.0 33.0 40.0 25.0 41.0 38-39 34.55987085496824 37.0 33.0 40.0 25.0 41.0 40-41 34.301889323039994 37.0 33.0 40.0 24.5 41.0 42-43 33.93748754210944 37.0 33.0 40.0 23.5 41.0 44-45 33.689487519490854 37.0 32.5 40.0 23.0 41.0 46-47 33.26065900650708 37.0 32.0 40.0 21.0 41.0 48-49 32.8577863759802 36.5 31.0 40.0 19.5 41.0 50-51 32.54381360592494 36.0 31.0 39.0 18.0 41.0 52-53 32.13959711358628 36.0 31.0 39.0 15.0 40.0 54-55 31.889844681028524 35.0 30.0 39.0 14.0 40.0 56-57 31.473955940417 35.0 30.0 39.0 12.5 40.0 58-59 31.0226201718164 35.0 29.0 38.0 8.5 40.0 60-61 30.685623099509037 34.5 28.5 38.0 8.0 40.0 62-63 30.28058473980856 34.0 28.0 38.0 7.0 40.0 64-65 29.827850683434576 34.0 28.0 37.0 4.0 40.0 66-67 28.18060795919608 33.0 24.5 36.5 2.0 39.0 68-69 24.6843340170742 29.5 13.0 35.0 2.0 38.0 70-71 24.084391164333887 28.5 10.5 34.0 2.0 37.0 72-73 25.81363437487189 31.0 20.0 35.0 2.0 37.0 74-75 26.391815501644793 31.0 22.0 35.0 2.0 37.0 76-77 26.400947400488842 30.5 24.0 34.0 2.0 36.0 78-79 27.21130437825054 32.0 26.0 35.0 2.0 36.0 80-81 27.07895457623381 32.0 25.5 35.0 2.0 36.0 82-83 26.69094677140955 32.0 25.0 35.0 2.0 36.0 84-85 26.376922402715362 32.0 24.5 35.0 2.0 35.0 86-87 25.950774191633105 32.0 23.5 34.0 2.0 35.0 88-89 25.460206493512 31.5 21.0 34.0 2.0 35.0 90-91 25.00931832543332 31.0 19.5 34.0 2.0 35.0 92-93 24.47688522342211 31.0 17.5 34.0 2.0 35.0 94-95 23.160148568738578 30.0 4.5 34.0 2.0 35.0 96-97 22.355066138145816 29.5 2.0 34.0 2.0 35.0 98-99 22.013001620055896 30.0 2.0 34.0 2.0 35.0 100-101 20.80170165949705 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.36324054381400117 1101 2 0.33297258884244485 1101 3 0.3313956789327541 1101 4 0.28754648049353193 1101 5 0.35689479978619687 1101 6 0.48018192621844236 1101 7 0.46720355676463754 1101 8 0.5855907183154727 1101 9 0.4897515405783395 1101 10-11 0.5850060631394953 1101 12-13 0.6520677474179664 1101 14-15 0.9001065959005459 1101 16-17 0.7973878942275476 1101 18-19 0.699276653800645 1101 20-21 0.711704143133268 1101 22-23 0.8085735635291869 1101 24-25 0.6762864488193756 1101 26-27 0.7700789749981354 1101 28-29 0.719040444019079 1101 30-31 0.7374320713795868 1101 32-33 0.7650165729432956 1101 34-35 0.8104140437964702 1101 36-37 0.9565008975766389 1101 38-39 0.9844135412279797 1101 40-41 1.0463293396346174 1101 42-43 1.001569350837137 1101 44-45 0.8273202568070204 1101 46-47 0.9886060613650329 1101 48-49 0.8842546439503991 1101 50-51 0.8258089621744276 1101 52-53 0.9141252952872065 1101 54-55 0.6766127194894551 1101 56-57 0.5466681013745607 1101 58-59 0.5420734311972808 1101 60-61 0.371373417662447 1101 62-63 0.3551504429354928 1101 64-65 -0.1078366501150434 1101 66-67 -2.9278434358643253 1101 68-69 -7.59585926957044 1101 70-71 -10.906928486813523 1101 72-73 -11.011697903199005 1101 74-75 -9.641114644662863 1101 76-77 -4.752099729850613 1101 78-79 -1.781125169304211 1101 80-81 -1.1815407762810395 1101 82-83 -0.9360457436875471 1101 84-85 -0.7764074876626772 1101 86-87 -0.8307501837001681 1101 88-89 -0.7742651975718537 1101 90-91 -0.9043365738307294 1101 92-93 -1.3986777026552168 1101 94-95 -5.195203103208289 1101 96-97 -2.0614488178661503 1101 98-99 -1.1548909801657992 1101 100-101 -0.9520267767291273 1102 1 0.4508739210713628 1102 2 0.2830254140180024 1102 3 0.40633542881822393 1102 4 0.23296192915191938 1102 5 0.3617571744539916 1102 6 0.4610571931705181 1102 7 0.37628784063284115 1102 8 0.47915748338864717 1102 9 0.6481710921054358 1102 10-11 0.6254808707002226 1102 12-13 0.6297101750887464 1102 14-15 0.5211967042574628 1102 16-17 0.6005659876173652 1102 18-19 0.618162844906827 1102 20-21 0.7470450416727132 1102 22-23 0.7005340064396961 1102 24-25 0.7016205271709808 1102 26-27 0.6965320734202223 1102 28-29 0.5973275846833062 1102 30-31 0.38070436326343327 1102 32-33 0.3528672654876033 1102 34-35 0.6379049434444397 1102 36-37 0.7957287434355678 1102 38-39 0.8171482148478617 1102 40-41 0.6676150317497829 1102 42-43 0.8632002625537964 1102 44-45 0.6278715791046849 1102 46-47 0.6146009647727766 1102 48-49 0.37601570557289676 1102 50-51 0.3856728481712679 1102 52-53 0.5891723747782862 1102 54-55 0.4633257226045231 1102 56-57 0.4076188601451527 1102 58-59 0.35601477758181943 1102 60-61 0.2655223242921636 1102 62-63 0.21718349768818968 1102 64-65 0.24802278465008598 1102 66-67 -2.6677467810490256 1102 68-69 -7.761535176087989 1102 70-71 -10.85882596755862 1102 72-73 -9.061123301824384 1102 74-75 -7.540354546032457 1102 76-77 -4.289367161335381 1102 78-79 -1.5956988153696088 1102 80-81 -1.0146357762075056 1102 82-83 -0.7728647922724434 1102 84-85 -0.7757675599957388 1102 86-87 -0.8094090542357932 1102 88-89 -0.8088586083528391 1102 90-91 -0.7547180719145175 1102 92-93 -1.136435717912505 1102 94-95 -4.742473458571464 1102 96-97 -1.6842325586436964 1102 98-99 -0.9119353429962764 1102 100-101 -0.7568250763138344 1103 1 0.29429937730383315 1103 2 0.23953649616062833 1103 3 0.3354071504199432 1103 4 0.21354581002641027 1103 5 0.3914091045100818 1103 6 0.41083091102517955 1103 7 0.3944535982412418 1103 8 0.38620115126277454 1103 9 0.6283503070556691 1103 10-11 0.6022681328304529 1103 12-13 0.4635500741099108 1103 14-15 0.7248823870774075 1103 16-17 0.6654133044183084 1103 18-19 0.5685860213551663 1103 20-21 0.5646223427238013 1103 22-23 0.590795965340817 1103 24-25 0.7340111878588687 1103 26-27 0.7817423449163954 1103 28-29 0.7331003704387271 1103 30-31 0.6794421172689624 1103 32-33 0.6678275961411231 1103 34-35 0.8292153443658492 1103 36-37 0.7292664205153443 1103 38-39 0.6839057307991396 1103 40-41 0.9685452120885074 1103 42-43 0.877699802017041 1103 44-45 0.5983219552096983 1103 46-47 0.5980841916511608 1103 48-49 0.6240611278121548 1103 50-51 0.6820419760096499 1103 52-53 0.8269296138138174 1103 54-55 0.5290725384975374 1103 56-57 0.6085758839242814 1103 58-59 0.5380497732860263 1103 60-61 0.37195857981457436 1103 62-63 0.4519133224891547 1103 64-65 0.20827715911311273 1103 66-67 -3.2666748775225685 1103 68-69 -7.66286247395858 1103 70-71 -10.95967699579385 1103 72-73 -8.851745314802532 1103 74-75 -7.187338178742948 1103 76-77 -3.9707437400307626 1103 78-79 -1.2071098537485199 1103 80-81 -0.6120132717357194 1103 82-83 -0.44994991905575077 1103 84-85 -0.8114319118220727 1103 86-87 -0.5005624443545251 1103 88-89 -0.25838960086466756 1103 90-91 -0.32232076397657394 1103 92-93 -0.20611114340011838 1103 94-95 0.5589960819689175 1103 96-97 0.555444537667853 1103 98-99 0.284141729906775 1103 100-101 0.4023636284074712 1104 1 0.4115304967666269 1104 2 0.28879828502750016 1104 3 0.39430040794457 1104 4 0.25786698117771323 1104 5 0.48363150658195053 1104 6 0.5209165786358483 1104 7 0.6608333469307226 1104 8 0.5557204922196988 1104 9 0.7051491687599807 1104 10-11 0.798465030604369 1104 12-13 0.7933424441666901 1104 14-15 0.961090756854567 1104 16-17 0.8736963757860678 1104 18-19 0.876750991840872 1104 20-21 0.9873378869761069 1104 22-23 0.84390104173346 1104 24-25 0.8928661845674739 1104 26-27 1.0243824431670063 1104 28-29 1.1289249482382147 1104 30-31 1.0377809258450412 1104 32-33 0.8528296163288474 1104 34-35 0.9279455180229306 1104 36-37 0.9398814979815313 1104 38-39 0.8778685110767768 1104 40-41 1.0410875840177667 1104 42-43 1.2358791385133259 1104 44-45 1.1070366414543642 1104 46-47 1.0509293483186894 1104 48-49 1.0724942756183253 1104 50-51 1.0809353770903627 1104 52-53 1.2563735170911414 1104 54-55 1.1136182271963833 1104 56-57 0.8928249836820292 1104 58-59 1.0776858149330977 1104 60-61 0.7712468121274298 1104 62-63 0.8163837441112776 1104 64-65 0.631044872476977 1104 66-67 -2.5533689037587948 1104 68-69 -7.155685554197689 1104 70-71 -8.482217640889099 1104 72-73 -7.899876547528994 1104 74-75 -6.79415420060009 1104 76-77 -3.385851488230905 1104 78-79 -0.5086905414195897 1104 80-81 0.322749176240805 1104 82-83 0.3697933173723733 1104 84-85 0.5309669185911794 1104 86-87 0.7884774234573726 1104 88-89 0.6520958496226363 1104 90-91 0.49131218476516736 1104 92-93 0.7152297473632743 1104 94-95 1.5430300452568453 1104 96-97 1.7214573133267344 1104 98-99 1.4444172556341037 1104 100-101 1.3840428558843563 1105 1 0.4628318374172693 1105 2 0.34182155757165233 1105 3 0.5209004788567171 1105 4 0.342413888534594 1105 5 0.4099011680021434 1105 6 0.5834040533926625 1105 7 0.5887091170725967 1105 8 0.549879284041161 1105 9 0.7454268566129372 1105 10-11 0.859632049262494 1105 12-13 0.8614356782230246 1105 14-15 1.0364681584896616 1105 16-17 0.9724777145868302 1105 18-19 0.9382130666891513 1105 20-21 0.9865934122141553 1105 22-23 1.1241433762846356 1105 24-25 1.096361536738776 1105 26-27 1.0567446911848108 1105 28-29 1.2214622670396977 1105 30-31 1.0893520409642292 1105 32-33 1.2517488227294393 1105 34-35 1.3433472250530016 1105 36-37 1.2425801676080113 1105 38-39 1.152625688362285 1105 40-41 1.1322764929470424 1105 42-43 1.2342725159355155 1105 44-45 0.9440990801127853 1105 46-47 0.9547043409962441 1105 48-49 0.9190165487123068 1105 50-51 1.0056112623360107 1105 52-53 1.286515752468084 1105 54-55 1.231665471129034 1105 56-57 1.3518301582132572 1105 58-59 1.4276556001926224 1105 60-61 1.4546535241162033 1105 62-63 1.4410538741682686 1105 64-65 1.2924342366827872 1105 66-67 1.9843702320379677 1105 68-69 -5.082247445599336 1105 70-71 -5.343233020603087 1105 72-73 -1.5592713277784185 1105 74-75 1.0964682847164724 1105 76-77 1.2648349721739116 1105 78-79 1.176950108711189 1105 80-81 1.2316404802535104 1105 82-83 1.3508699487371238 1105 84-85 1.2024471839464859 1105 86-87 1.5112515271638145 1105 88-89 1.5168677745790866 1105 90-91 1.4517598048404992 1105 92-93 1.5031595751700841 1105 94-95 2.3532628220839875 1105 96-97 2.344313942327748 1105 98-99 2.1740663432825436 1105 100-101 2.058354365747821 1106 1 0.2247792114451812 1106 2 0.194628631184095 1106 3 0.20123924523575099 1106 4 0.11324375963292255 1106 5 0.3673998363732949 1106 6 0.37838647589186536 1106 7 0.40399408564615413 1106 8 0.41824404381531366 1106 9 0.6213062643044225 1106 10-11 0.6179435438827099 1106 12-13 0.46954050404597325 1106 14-15 0.574577245524921 1106 16-17 0.5301489620567352 1106 18-19 0.35352679844583434 1106 20-21 0.5988316139825614 1106 22-23 0.5664124718423196 1106 24-25 0.608301453645133 1106 26-27 0.5957646123523759 1106 28-29 0.8262550655908854 1106 30-31 0.6549107924290212 1106 32-33 0.6670284647876059 1106 34-35 0.777321870839728 1106 36-37 0.6495280742874598 1106 38-39 0.6868783782525583 1106 40-41 0.8320740853620876 1106 42-43 0.7981970924775439 1106 44-45 0.8817138665195188 1106 46-47 0.7983668529809052 1106 48-49 0.6077818624440638 1106 50-51 0.6180113901723772 1106 52-53 0.866254753213795 1106 54-55 0.6903721931914504 1106 56-57 0.5824767971820464 1106 58-59 0.8198607775655837 1106 60-61 0.7875681933395988 1106 62-63 0.7989552271031783 1106 64-65 0.9315628187643874 1106 66-67 1.689355850446404 1106 68-69 -4.967340659618724 1106 70-71 -5.970172404857909 1106 72-73 -2.080210925093855 1106 74-75 0.8451451782926931 1106 76-77 0.9687084141681623 1106 78-79 0.753516636888456 1106 80-81 0.83832951828483 1106 82-83 0.9699623105140525 1106 84-85 0.9525100368281301 1106 86-87 0.9589557990291624 1106 88-89 0.8706581229331896 1106 90-91 0.8208308605558514 1106 92-93 0.8965696103250842 1106 94-95 1.7329080761587718 1106 96-97 1.7679679318877781 1106 98-99 1.4458123750285772 1106 100-101 1.2444798584505001 1107 1 0.08045742370973841 1107 2 0.012427388832691122 1107 3 -0.10830638520580749 1107 4 -0.048545957832324405 1107 5 0.04779087551235506 1107 6 0.10742209395303348 1107 7 0.07578550628326752 1107 8 0.053263330707721934 1107 9 0.032963527243992985 1107 10-11 0.09501960724816882 1107 12-13 0.15563613232617257 1107 14-15 0.09837915015413756 1107 16-17 -0.06211485397914629 1107 18-19 0.23155160006984943 1107 20-21 0.1822277407644748 1107 22-23 0.118167749575953 1107 24-25 0.3947508606811354 1107 26-27 0.2553415021656633 1107 28-29 0.21587211314918875 1107 30-31 0.1852996080113769 1107 32-33 0.18710816659672957 1107 34-35 0.12311100903994543 1107 36-37 0.2093762691170511 1107 38-39 0.25010860800868073 1107 40-41 0.3208477155760008 1107 42-43 0.324202546981347 1107 44-45 0.18008407720385122 1107 46-47 -0.016783850777386533 1107 48-49 0.17757464250880162 1107 50-51 0.310005559789019 1107 52-53 0.36557794194545323 1107 54-55 0.30219283830901134 1107 56-57 0.42528911645327483 1107 58-59 0.3665149363370688 1107 60-61 0.34835987074827557 1107 62-63 0.4657285622941387 1107 64-65 0.3685171079327034 1107 66-67 1.2948640305953418 1107 68-69 -5.26677854033802 1107 70-71 -5.662108471745807 1107 72-73 -2.1400111761017904 1107 74-75 0.2502975790031101 1107 76-77 0.35167277703312294 1107 78-79 0.2833652681364036 1107 80-81 0.20815701485956595 1107 82-83 0.4489404737359628 1107 84-85 0.5024553186740484 1107 86-87 0.5266905192813667 1107 88-89 0.42269033454696014 1107 90-91 0.47059061795285473 1107 92-93 0.5656475661498028 1107 94-95 1.4218025870006556 1107 96-97 1.4581732527293845 1107 98-99 1.3352444371820162 1107 100-101 1.1204291331615472 1108 1 0.11278925127712469 1108 2 0.03900312938737116 1108 3 0.08798526399028006 1108 4 0.036544744128860884 1108 5 0.019254632965363783 1108 6 0.1855643321383127 1108 7 0.253687824002526 1108 8 0.20715377107174504 1108 9 0.3036924392687652 1108 10-11 0.3014400244850961 1108 12-13 0.37655762944253723 1108 14-15 0.2828599509790095 1108 16-17 0.12120400184969782 1108 18-19 0.21154252855519928 1108 20-21 0.30537777148631307 1108 22-23 0.22725603598265565 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0.8401165328675084 2203 98-99 0.4670517218311261 2203 100-101 0.0971560424468727 2204 1 0.29252658711202173 2204 2 0.2595309592810544 2204 3 0.25616787812411346 2204 4 0.2266385527592547 2204 5 0.1673321814331601 2204 6 0.39226852225713316 2204 7 0.401505209868688 2204 8 0.4591637773256565 2204 9 0.645768736954814 2204 10-11 0.4394025010329372 2204 12-13 0.585127985915328 2204 14-15 0.6306097946312903 2204 16-17 0.6262383983475175 2204 18-19 0.6039615404376235 2204 20-21 0.6218192974400409 2204 22-23 0.5847807389219923 2204 24-25 0.7170208773981201 2204 26-27 0.750944935385057 2204 28-29 0.7185733781508077 2204 30-31 0.5920724316846204 2204 32-33 0.6460345370151543 2204 34-35 0.7532568709626446 2204 36-37 0.8132541602820069 2204 38-39 0.6443961401327343 2204 40-41 0.8036220362925874 2204 42-43 0.9143164719794683 2204 44-45 0.8341625721762753 2204 46-47 0.8103819966738968 2204 48-49 0.7792119089076692 2204 50-51 0.737244961969715 2204 52-53 0.8822178281701625 2204 54-55 0.651235678699237 2204 56-57 0.48464578702889227 2204 58-59 0.6201684207054434 2204 60-61 0.4649099343726135 2204 62-63 0.49649953961393223 2204 64-65 0.6562535285020807 2204 66-67 1.8725826862504178 2204 68-69 4.398055362703474 2204 70-71 -0.6592867446568107 2204 72-73 1.1431975366904474 2204 74-75 1.3495012517494374 2204 76-77 0.934827646166589 2204 78-79 0.6524275201886027 2204 80-81 0.59971374032677 2204 82-83 0.3318589597261372 2204 84-85 0.3954850349843362 2204 86-87 0.26510989102217764 2204 88-89 0.299168140879452 2204 90-91 0.37771829079898467 2204 92-93 0.560463604474112 2204 94-95 0.9422005510217168 2204 96-97 0.9993273733411776 2204 98-99 0.711358162574367 2204 100-101 0.32376584315930046 2205 1 0.5653844359013469 2205 2 0.5544893798754487 2205 3 0.6396666662738681 2205 4 0.43472006439537125 2205 5 0.6466477095880876 2205 6 0.6589113441033803 2205 7 0.7559923501374115 2205 8 0.683668754147547 2205 9 0.9426682828051085 2205 10-11 0.9100181680212032 2205 12-13 0.9338920869106957 2205 14-15 1.1465286594923398 2205 16-17 1.0692242561727667 2205 18-19 1.0986817795133277 2205 20-21 1.1583802311530107 2205 22-23 1.2250794423598848 2205 24-25 1.2878451708776595 2205 26-27 1.4457946894853464 2205 28-29 1.4410255041832372 2205 30-31 1.4750697256112488 2205 32-33 1.2996258493430872 2205 34-35 1.4913116042379357 2205 36-37 1.6094833999930387 2205 38-39 1.4987046795590473 2205 40-41 1.5952281261342236 2205 42-43 1.6840186156602002 2205 44-45 1.480956087012622 2205 46-47 1.3761951128938676 2205 48-49 1.5271032894757042 2205 50-51 1.6419016671488933 2205 52-53 1.6882128395827252 2205 54-55 1.738804589445877 2205 56-57 1.7393812271776063 2205 58-59 1.8018199725796933 2205 60-61 1.8060627216281802 2205 62-63 1.914149961996685 2205 64-65 1.9576563569370258 2205 66-67 2.938352081737161 2205 68-69 5.767682536386321 2205 70-71 5.838707090202121 2205 72-73 3.92409157626971 2205 74-75 2.8247368683812013 2205 76-77 2.074191554207154 2205 78-79 1.7169925953703782 2205 80-81 1.6554591811814205 2205 82-83 1.6453147330748443 2205 84-85 1.5481091260966515 2205 86-87 1.767575241517509 2205 88-89 1.586698507728876 2205 90-91 1.6395341836855408 2205 92-93 1.7742251405825478 2205 94-95 2.4542651673475753 2205 96-97 2.5044621357949985 2205 98-99 2.1484227553691504 2205 100-101 1.6716333161573935 2206 1 0.2010593506447833 2206 2 0.08842649584325812 2206 3 0.09085638714595845 2206 4 0.22990066102136808 2206 5 0.276594289695268 2206 6 0.36254867420394987 2206 7 0.34643398479746423 2206 8 0.3000821059106471 2206 9 0.4471552516746655 2206 10-11 0.4956902503206848 2206 12-13 0.4694912337785766 2206 14-15 0.480083678771841 2206 16-17 0.3876628997720175 2206 18-19 0.41312488326763486 2206 20-21 0.33378036106777387 2206 22-23 0.449394699035949 2206 24-25 0.480629614340188 2206 26-27 0.6920489239890415 2206 28-29 0.5692400448174766 2206 30-31 0.5434746159201325 2206 32-33 0.6946147839287349 2206 34-35 0.7378489887214315 2206 36-37 0.8786824603769716 2206 38-39 0.848004877074807 2206 40-41 0.8328276393315193 2206 42-43 0.8261331861295176 2206 44-45 0.6876440536577562 2206 46-47 0.6888269159521485 2206 48-49 0.7492237797528674 2206 50-51 0.5750336609250795 2206 52-53 0.8718949411806065 2206 54-55 1.051956476420031 2206 56-57 1.099943032992801 2206 58-59 1.0698160940197852 2206 60-61 1.0529251290544757 2206 62-63 1.122019482633874 2206 64-65 1.1021785047603885 2206 66-67 2.074052771720506 2206 68-69 5.075587651402426 2206 70-71 5.100144404441007 2206 72-73 2.92217047116338 2206 74-75 2.037416256497778 2206 76-77 1.1900289757012494 2206 78-79 0.9242252409863205 2206 80-81 0.9470769881900196 2206 82-83 0.7519634231639323 2206 84-85 0.5759304290963989 2206 86-87 0.7784693402149614 2206 88-89 0.740487704032347 2206 90-91 0.6481575120715419 2206 92-93 0.6786269473041209 2206 94-95 1.4169180249058577 2206 96-97 1.4799664996469701 2206 98-99 1.2239208406370423 2206 100-101 1.0063878754480022 2207 1 0.08785586895836417 2207 2 0.06482254811029975 2207 3 0.07316227382985474 2207 4 0.09535334856904143 2207 5 0.05368239070743641 2207 6 0.24741383191212662 2207 7 0.11811425713866441 2207 8 0.15815774738879185 2207 9 0.4820282843548469 2207 10-11 0.25632174717259915 2207 12-13 0.19830011105425882 2207 14-15 0.29464649448231484 2207 16-17 0.14623390545337855 2207 18-19 0.2366523583808302 2207 20-21 0.325607753066798 2207 22-23 0.28613187042191157 2207 24-25 0.4045008367256031 2207 26-27 0.33734116135398295 2207 28-29 0.24576427885784824 2207 30-31 0.19941552326872625 2207 32-33 0.1544360125761699 2207 34-35 0.2764436098309062 2207 36-37 0.3939346682155076 2207 38-39 0.31028679727761954 2207 40-41 0.3355808908410509 2207 42-43 0.5240041921903966 2207 44-45 0.3634327842881717 2207 46-47 0.39618019478687927 2207 48-49 0.3632569324362436 2207 50-51 0.24075537839005534 2207 52-53 0.6277708088507126 2207 54-55 0.5516966023436822 2207 56-57 0.6718364039741935 2207 58-59 0.6645558973542158 2207 60-61 0.7163084067886132 2207 62-63 0.6411336052101859 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58-59 0.8663376622406744 2311 60-61 0.9293024627679429 2311 62-63 0.8528805483882209 2311 64-65 0.8775059373947975 2311 66-67 1.9138047350204062 2311 68-69 4.475354496040396 2311 70-71 4.79219379579644 2311 72-73 2.869227789153168 2311 74-75 2.031414800761656 2311 76-77 1.3448383045075474 2311 78-79 1.0003928536654811 2311 80-81 0.81598784271144 2311 82-83 0.8366972005615771 2311 84-85 0.6792529200034458 2311 86-87 0.9230374358356599 2311 88-89 0.9987885316852854 2311 90-91 0.8969081492260713 2311 92-93 1.0750156850038017 2311 94-95 1.7140749117904086 2311 96-97 1.8578697644699922 2311 98-99 1.5361519398258032 2311 100-101 1.503702539073604 2312 1 -0.08095069583894698 2312 2 -0.13874524404777944 2312 3 -0.09159100626634853 2312 4 -0.02322199604866171 2312 5 0.04283735462254157 2312 6 0.17889920151939265 2312 7 0.18277376587408867 2312 8 0.1780535658996527 2312 9 -1.4707134942642597 2312 10-11 -1.0890338462455134 2312 12-13 -2.0825049425597015 2312 14-15 -1.943822953906718 2312 16-17 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82-83 -7.116098415569869 2315 84-85 -5.1085446036987285 2315 86-87 -5.466628708015328 2315 88-89 -3.9933852163889014 2315 90-91 -3.336347097635816 2315 92-93 -4.1531089262722 2315 94-95 -2.5093592992571025 2315 96-97 -1.3140951306692124 2315 98-99 -2.8983735615108834 2315 100-101 -1.9538163125201713 2316 1 -0.33929675978728113 2316 2 -0.33881984863287684 2316 3 -1.0781421512912068 2316 4 -0.3251509463744 2316 5 -0.317666327653356 2316 6 -2.1064734691197415 2316 7 -0.27516642650959966 2316 8 -0.28093099641814945 2316 9 -0.02289629105018065 2316 10-11 -0.06841496280233628 2316 12-13 -0.7247560451923789 2316 14-15 -0.9853089707311611 2316 16-17 -1.539705511069421 2316 18-19 -0.30568966639639683 2316 20-21 -4.264749183842952 2316 22-23 -4.207711746068135 2316 24-25 -2.774032835782357 2316 26-27 -2.158942238729466 2316 28-29 -1.3443098067627588 2316 30-31 -0.3694903680091386 2316 32-33 -1.9380461334929677 2316 34-35 -2.096731765858891 2316 36-37 -4.318123766139401 2316 38-39 -2.1623673687523066 2316 40-41 -1.4570289690666485 2316 42-43 -2.733366487282325 2316 44-45 -0.6724125980699256 2316 46-47 -0.7112983031205928 2316 48-49 -0.2721826996268035 2316 50-51 -0.010248449804471704 2316 52-53 -2.4482584582464106 2316 54-55 -0.5905793674384903 2316 56-57 0.3216330135268528 2316 58-59 0.34126307356437025 2316 60-61 -1.1983986847191375 2316 62-63 -0.42025953682560413 2316 64-65 0.5452883030517839 2316 66-67 1.3564897660747377 2316 68-69 3.8886991149700627 2316 70-71 4.1616579303643455 2316 72-73 2.4631956831223256 2316 74-75 1.4421743387462556 2316 76-77 0.15850206289705682 2316 78-79 0.49161606030223126 2316 80-81 0.050930809271445554 2316 82-83 0.4007524601492314 2316 84-85 0.602864089664596 2316 86-87 0.20919942642129996 2316 88-89 0.5490314603781812 2316 90-91 0.5884121699946974 2316 92-93 0.9372482279177241 2316 94-95 1.4254100445140772 2316 96-97 1.451454221872428 2316 98-99 0.5761473796433094 2316 100-101 0.32415928313995934 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 41.0 4 142.0 5 335.0 6 834.0 7 1923.0 8 3937.0 9 6162.0 10 9701.0 11 13867.0 12 17784.0 13 22081.0 14 26721.0 15 33352.0 16 39828.0 17 44345.0 18 39960.0 19 33343.0 20 32147.0 21 32551.0 22 33920.0 23 38800.0 24 46733.0 25 56418.0 26 70015.0 27 86811.0 28 104553.0 29 124317.0 30 145408.0 31 168756.0 32 193540.0 33 224732.0 34 260775.0 35 296662.0 36 295925.0 37 230946.0 38 87252.0 39 4906.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.08133903637456 19.266145851744497 14.99388454876454 21.658630563116404 2 16.50749246370418 21.736734422632075 37.28854539593125 24.467227717732495 3 20.29473425287291 25.452901751950503 29.8831043437572 24.369259651419387 4 13.399848455504301 17.180968442837894 35.304990365897964 34.114192735759836 5 13.72436896741472 38.44735289866264 32.26222456104885 15.56605357287379 6 31.803846007042864 35.70106293196189 16.398754281626786 16.09633677936846 7 28.438236429204544 34.24142225640141 19.10453742880448 18.21580388558956 8 27.662560451693068 32.90088254877485 18.65410958419979 20.78244741533229 9 27.07005964237195 16.916012966101814 19.833032459078037 36.18089493244819 10-11 25.929287952919424 26.560293363001374 25.233148096575686 22.277270587503516 12-13 27.609071040723343 24.181472413105773 26.515904396910866 21.69355214926002 14-15 23.784003856468136 25.217315089562515 26.403429966510362 24.595251087458987 16-17 22.0418345983282 28.757292746059054 26.666693761918964 22.534178893693777 18-19 21.731645610778042 26.55925875072694 30.17308574838571 21.53600989010931 20-21 23.070378094461592 25.118509039130515 30.030171077821734 21.780941788586166 22-23 22.437668137345682 25.225019543868033 30.24646832055619 22.090843998230095 24-25 22.518388199886058 25.667619238799915 30.23035258127492 21.583639980039102 26-27 22.420336198841074 25.50552815393163 30.63472692856305 21.43940871866425 28-29 21.6257377578242 26.204528720460967 30.653963522941407 21.515769998773422 30-31 21.760559920633376 25.933050847158107 30.30282734755519 22.003561884653326 32-33 21.701689686641345 26.025949339797165 30.15710346299874 22.11525751056275 34-35 22.59959293163623 25.682772617309613 29.99139879427602 21.726235656778137 36-37 22.595081979972164 25.469227705006535 30.175372682870915 21.760317632150386 38-39 22.301257524539327 25.758238298158375 30.18524120095451 21.755262976347783 40-41 22.008826487601077 25.97885287506601 30.2960119809529 21.716308656380008 42-43 22.04858402436595 26.228088702655906 29.356477309754197 22.366849963223938 44-45 22.0491219852847 26.179442024283993 29.24693938341214 22.524496607019167 46-47 22.490618435890067 26.545329117562517 28.615915271850884 22.348137174696532 48-49 22.581813209585416 26.494331630525863 28.428205340927367 22.495649818961358 50-51 22.144821076822062 26.894832397491342 28.617431425045847 22.342915100640752 52-53 22.695882932157016 27.0394701473322 27.824887032106364 22.439759888404414 54-55 21.88252052833474 26.570449878115497 28.32354968406929 23.223479909480467 56-57 22.564992774406818 27.013059099607123 27.743626231333884 22.678321894652175 58-59 22.887619974044764 26.463366549415067 27.712385009869738 22.936628466670424 60-61 22.568640604643832 26.488918468229972 28.629976580796253 22.312464346329943 62-63 22.63198342742261 26.496243939065415 28.275696692711964 22.596075940800006 64-65 22.24868634157505 26.921661160507988 28.207513410493185 22.622139087423783 66-67 21.92782234940751 26.65346196648895 28.592239142407777 22.82647654169577 68-69 21.750856647797082 26.434161464795597 29.1378519879323 22.67712989947502 70-71 21.45676250239552 26.636651706725733 29.16846200813809 22.738123782740654 72-73 22.333676312915742 26.44480665891877 28.15381597713123 23.067701051034266 74-75 21.745508072312546 27.437563032452232 27.917684727224657 22.89924416801056 76-77 22.257411743913753 26.84448371846419 27.63448062563513 23.263623911986922 78-79 22.030998252740925 26.955964370547576 27.90438302774708 23.108654348964414 80-81 22.036287785934718 27.514762367237655 27.61827918227017 22.830670664557452 82-83 22.114734874883904 27.116180343604523 27.50500789529859 23.264076886212983 84-85 21.8983740067262 27.034682767326895 27.3613622323409 23.70558099360601 86-87 22.149660022622157 26.874673200622084 27.533900106607945 23.441766670147814 88-89 21.966394516927586 26.916229444088962 27.137504623152747 23.9798714158307 90-91 21.786479015307492 26.896586823183384 27.451556964711816 23.865377196797308 92-93 21.941459071871776 26.9775247522478 27.55370622378835 23.527309952092082 94-95 21.39406602117259 26.720189050874367 27.880310990388423 24.005433937564614 96-97 20.922586589014635 26.56832175483575 28.022827344783018 24.486264311366597 98-99 21.569855572029034 26.66918570095752 27.122806279606092 24.638152447407354 100-101 21.493287715897154 26.25694621778497 27.54202191156733 24.707744154750554 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10664.0 1 6941.0 2 3544.5 3 4072.5 4 3879.5 5 3797.0 6 5610.0 7 10037.0 8 14184.0 9 15419.5 10 13279.0 11 10162.5 12 8790.0 13 8198.5 14 7812.5 15 7415.0 16 7123.5 17 7079.0 18 7212.5 19 7394.0 20 7640.0 21 8367.5 22 9401.5 23 10508.0 24 11812.5 25 13303.5 26 15272.5 27 17860.5 28 20894.0 29 24064.5 30 26878.5 31 30347.5 32 34643.5 33 37518.0 34 39965.5 35 43007.0 36 46240.0 37 49082.5 38 51224.0 39 53229.5 40 55854.5 41 61093.5 42 67131.0 43 71536.0 44 75417.0 45 80343.5 46 87228.5 47 102398.5 48 129135.0 49 131074.0 50 111414.0 51 103238.5 52 100943.0 53 102166.5 54 102821.5 55 99463.5 56 93344.5 57 86578.5 58 80240.5 59 73357.0 60 65199.5 61 54983.5 62 44177.0 63 34088.5 64 26339.0 65 20935.0 66 16083.5 67 12423.5 68 8822.5 69 5717.0 70 4045.5 71 3046.0 72 2462.5 73 2165.5 74 1793.5 75 1414.5 76 1016.0 77 593.5 78 337.5 79 208.0 80 138.0 81 92.5 82 61.5 83 32.5 84 13.0 85 6.5 86 3.5 87 3.0 88 2.5 89 2.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02233584917929891 2 0.01964989263242119 3 0.0 4 0.0 5 1.0602460053464671E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.827322680923912E-4 18-19 0.001360649040194633 20-21 3.53415335115489E-5 22-23 0.0 24-25 0.0 26-27 5.654645361847825E-4 28-29 0.01841293895951698 30-31 0.0046297408900129065 32-33 0.008835383377887227 34-35 0.009807275549454823 36-37 0.10128883504409918 38-39 0.10415149925853463 40-41 0.11943671250227952 42-43 0.10478764686174251 44-45 0.09945107530149862 46-47 0.10052899207360086 48-49 0.10339165628803632 50-51 0.10051132130684509 52-53 0.07704454305517662 54-55 0.06555854466392322 56-57 0.03691423175281283 58-59 0.015214530176721806 60-61 0.008128552707656249 62-63 0.001360649040194633 64-65 0.004400020922187838 66-67 0.4098204225999211 68-69 0.7905547631198375 70-71 0.877159190989888 72-73 0.27186474653758996 74-75 0.1449886412311294 76-77 0.014384004139200405 78-79 0.008605663410062159 80-81 0.0014136613404619562 82-83 0.0 84-85 0.0 86-87 1.9437843431351897E-4 88-89 8.305260375213993E-4 90-91 0.003551824117910665 92-93 0.0014666736407292795 94-95 0.21788055409869903 96-97 0.22402998092970855 98-99 0.01433099183893308 100-101 0.00722734360311175 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2829532.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.53514532715543 #Duplication Level Percentage of deduplicated Percentage of total 1 64.58231451835766 22.303596174555423 2 14.517618396277914 10.027361222392859 3 6.705869125798763 6.947644944130352 4 3.870952535608202 5.347356334870003 5 2.4376998063057678 4.209315853737417 6 1.7145451466306332 3.5527239485314763 7 1.1652946988967037 2.8170535242753045 8 0.8794532579785701 2.4297636858184193 9 0.6522304193391885 2.027238508680336 >10 3.1603237265022184 18.547293589552307 >50 0.1790721740982039 4.277040890090077 >100 0.11881365806072992 7.895811940274172 >500 0.01019492462013309 2.4048001326340813 >1k 0.0047853727808766814 3.4153507799786356 >5k 3.120895291876097E-4 0.6190197544710078 >10k+ 5.201492153126827E-4 3.178628716008266 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26275 0.9285987930159475 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21577 0.7625642685786908 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16625 0.5875529946295006 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13632 0.4817757848294347 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10366 0.36635033638071596 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6402 0.22625649754093607 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5651 0.19971500587376284 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5177 0.1829631189892887 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4936 0.1744458094130054 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4880 0.17246668353635866 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4603 0.1626770787536596 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4279 0.15122642189591778 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4112 0.1453243857994891 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3119 0.11023024302252103 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3049 0.10775633567671261 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3037 0.10733223727457403 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2999 0.10598925900113516 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2863 0.10118281044356453 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 3.53415335115489E-5 3 0.0 0.0 0.0 0.0 3.53415335115489E-5 4 0.0 0.0 0.0 0.0 3.53415335115489E-5 5 0.0 0.0 0.0 0.0 3.53415335115489E-5 6 0.0 0.0 0.0 0.0 3.53415335115489E-5 7 0.0 0.0 0.0 0.0 3.53415335115489E-5 8 0.0 0.0 0.0 0.0 3.53415335115489E-5 9 0.0 0.0 0.0 0.0 3.53415335115489E-5 10-11 0.0 0.0 0.0 3.53415335115489E-5 3.53415335115489E-5 12-13 0.0 0.0 0.0 7.06830670230978E-5 7.06830670230978E-5 14-15 0.0 0.0 0.0 7.06830670230978E-5 2.827322680923912E-4 16-17 0.0 0.0 0.0 1.2369536729042117E-4 2.827322680923912E-4 18-19 0.0 0.0 0.0 2.297199678250679E-4 2.827322680923912E-4 20-21 0.0 0.0 0.0 5.83135302940557E-4 3.180738016039401E-4 22-23 0.0 0.0 0.0 0.0012369536729042118 3.5341533511548906E-4 24-25 0.0 0.0 0.0 0.002297199678250679 3.5341533511548906E-4 26-27 0.0 0.0 0.0 0.0037285317854684097 4.240984021385869E-4 28-29 0.0 0.0 0.0 0.006926940568263585 4.240984021385869E-4 30-31 0.0 0.0 0.0 0.01570931164588349 4.240984021385869E-4 32-33 0.0 0.0 0.0 0.028149531441948705 4.240984021385869E-4 34-35 0.0 0.0 0.0 0.04714560570440624 4.240984021385869E-4 36-37 0.0 0.0 0.0 0.07702687228842084 4.594399356501358E-4 38-39 0.0 0.0 0.0 0.11705115899024998 4.947814691616847E-4 40-41 0.0 0.0 0.0 0.1622176388180095 4.947814691616847E-4 42-43 0.0 0.0 0.0 0.20669495874229377 4.947814691616847E-4 44-45 0.0 0.0 0.0 0.2628879970256566 4.947814691616847E-4 46-47 0.0 0.0 0.0 0.3317863166064211 4.947814691616847E-4 48-49 0.0 0.0 0.0 0.4041834480048291 4.947814691616847E-4 50-51 0.0 0.0 0.0 0.48433804600902197 5.124522359174591E-4 52-53 0.0 0.0 0.0 0.5634147272411127 5.301230026732336E-4 54-55 0.0 0.0 0.0 0.6456191341889754 5.301230026732336E-4 56-57 0.0 0.0 0.0 0.744133658852418 5.654645361847825E-4 58-59 0.0 0.0 0.0 0.8410048022075736 6.008060696963314E-4 60-61 0.0 0.0 0.0 0.9432301878897288 6.008060696963314E-4 62-63 0.0 0.0 0.0 1.0426635924244716 6.008060696963314E-4 64-65 0.0 0.0 0.0 1.1436520244337225 6.008060696963314E-4 66-67 0.0 0.0 0.0 1.2506485171399369 6.008060696963314E-4 68-69 0.0 0.0 0.0 1.3841865015133243 6.008060696963314E-4 70-71 0.0 0.0 0.0 1.5597809107654552 6.008060696963314E-4 72-73 0.0 0.0 0.0 1.7484693581836148 6.184768364521059E-4 74-75 0.0 0.0 0.0 1.958026981140344 6.538183699636548E-4 76-77 0.0 0.0 0.0 2.184248137147769 6.714891367194292E-4 78-79 0.0 0.0 0.0 2.445581106698917 7.068306702309781E-4 80-81 0.0 0.0 0.0 2.73667164746679 7.068306702309781E-4 82-83 0.0 0.0 0.0 3.0329220521273483 7.068306702309781E-4 84-85 0.0 0.0 0.0 3.335551603586741 7.245014369867526E-4 86-87 0.0 0.0 0.0 3.6423691267672536 7.598429704983015E-4 88-89 0.0 0.0 0.0 3.9723353543978295 7.775137372540759E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 19295 0.0 25.156597 1 TACATGG 19215 0.0 24.662292 2 ACATGGG 19810 0.0 22.673225 3 AGTACTT 19130 0.0 22.291142 12-13 GAGTACT 18920 0.0 22.250566 12-13 GTACTTT 20065 0.0 21.075304 14-15 CATGGGA 12950 0.0 20.781075 4 GTATCAA 39485 0.0 20.576683 1 TACTTTT 21180 0.0 20.144781 14-15 ACTTTTT 22860 0.0 18.954498 16-17 TAAGGTG 1075 0.0 18.951756 5 GTAAGGT 855 0.0 18.840925 4 ATGGGAG 4225 0.0 18.839613 5 CATGGGG 7170 0.0 18.63457 4 GGTATCA 30845 0.0 18.57968 1 ACGCATA 365 0.0 18.496496 64-65 ATTCGCG 540 0.0 18.425478 80-81 ATGGGAT 5090 0.0 18.058155 5 TATCAAC 46065 0.0 17.570765 2 CAATTCG 580 0.0 17.564445 78-79 >>END_MODULE