FastQCFastQC Report
Fri 27 May 2016
SRR939390_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939390_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2818999
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187170.6639590861862668No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126460.44859895303261904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101340.3594893080841816No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA89100.3160696403226819No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT63220.22426400293153706No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT41420.14693158812755874No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA36860.13075563347131375No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33280.11805609012277053No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT30880.10954242977737841No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29700.10535654677422732No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA29490.1046116014940055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT3350.026.0825336
GTACATG169700.025.5706271
TACATGG170200.025.0973072
ACATGGG178350.023.2396833
GAGTACT160050.023.2228312-13
GTATCAA361950.022.6671411
AGTACTT164350.022.33711812-13
GTACTTT174000.021.66606914-15
ATGGGAG42450.020.6014335
CATGGGA116900.020.47784
CATGGGG70100.020.4078544
ACTTTTT208400.020.06849316-17
TACTTTT186200.020.02987914-15
TATCAAC413550.019.5682622
GTAAGGT7800.019.5636314
ATCAACG431500.018.976833
TCAACGC433900.018.9470564
CAACGCA433700.018.7977185
GTCGGGA8350.018.7730032
AACGCAG436950.018.6082346
Produced by FastQC (version 0.11.5)