FastQCFastQC Report
Fri 27 May 2016
SRR939390_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939390_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2818999
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255270.9055341984867679No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202800.7194042991856329No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163070.5784677468846211No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135150.47942549819989294No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT98820.35054996472151995No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT63140.2239802142533573No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55730.19769428793695917No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA50320.17850307857505449No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT48340.171479308790106No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46350.16442006542038504No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA44690.1585314503481555No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43040.15267830886069841No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC40100.1422490749375931No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30590.10851369581897688No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT30220.1072011731823956No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT29340.1040794977224185No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT29220.10365381470514888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG194250.024.6048341
TACATGG193250.024.3383272
ACGCATA4050.023.0856264-65
ACATGGG194750.022.9786513
CGCATAG4300.022.3027666-67
AGTACTT180700.022.09399812-13
GAGTACT178800.021.74536712-13
CATGGGA125850.021.537184
ATGGGAG44950.020.8908465
GTACTTT189250.020.53210314-15
GTATCAA380800.019.9173621
TACTTTT201600.019.76821914-15
ATAACGC3054.623871E-918.6711793
GGACCGT3054.658432E-918.6595846
ACTTTTT218300.018.4509416-17
CATGGGG72250.018.3961164
AACGCAT5550.018.1421364-65
CAATTCG6100.017.88099178-79
AGGTAAG8550.017.7631662
ATTCGCG6150.017.73388780-81
Produced by FastQC (version 0.11.5)