##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939390_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2818999 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.88716207419726 31.0 31.0 31.0 28.0 34.0 2 31.112825155312223 31.0 31.0 34.0 28.0 34.0 3 31.112397698615716 31.0 31.0 34.0 28.0 34.0 4 35.0232688979315 35.0 35.0 37.0 32.0 37.0 5 34.5980683214148 35.0 35.0 37.0 30.0 37.0 6 34.669917938956345 35.0 35.0 37.0 31.0 37.0 7 34.506323343853616 35.0 35.0 37.0 30.0 37.0 8 34.41221121398056 35.0 35.0 37.0 30.0 37.0 9 35.883390167928404 37.0 35.0 39.0 30.0 39.0 10-11 35.67734663971147 37.0 35.0 39.0 30.0 39.0 12-13 35.65026645983202 37.0 35.0 39.0 30.0 39.0 14-15 36.40845544819278 38.0 35.0 40.0 30.0 41.0 16-17 36.354252874867996 38.0 35.0 40.0 30.0 41.0 18-19 36.39530131085537 38.0 35.0 40.0 30.0 41.0 20-21 36.27926650559294 38.0 34.5 40.0 29.5 41.0 22-23 36.102643349642904 38.0 34.0 40.0 29.0 41.0 24-25 35.89955920523562 38.0 34.0 40.0 29.0 41.0 26-27 35.38011968078031 38.0 34.0 40.0 27.0 41.0 28-29 35.15824393694358 38.0 33.0 40.0 27.0 41.0 30-31 34.81794388717414 37.0 33.0 40.0 26.0 40.5 32-33 34.48743720732075 37.0 33.0 40.0 25.0 41.0 34-35 34.658001120255804 37.0 33.0 40.0 25.0 41.0 36-37 34.42911313554918 37.0 33.0 40.0 25.0 41.0 38-39 34.19133813101743 37.0 33.0 40.0 24.0 41.0 40-41 33.99164277816345 37.0 33.0 40.0 24.0 41.0 42-43 33.77144511225438 37.0 32.5 40.0 23.5 41.0 44-45 33.30956130172448 37.0 31.5 40.0 22.0 41.0 46-47 32.80720142149749 36.0 31.0 39.0 20.0 41.0 48-49 32.31726864748799 36.0 30.5 39.0 16.5 40.0 50-51 32.02073484240328 36.0 30.0 39.0 15.0 40.0 52-53 31.87812358216516 35.0 30.0 39.0 14.5 40.0 54-55 31.51241415835905 35.0 30.0 39.0 11.0 40.0 56-57 30.985442172913153 35.0 28.5 38.5 8.5 40.0 58-59 30.501540972522516 34.5 28.0 38.0 8.0 40.0 60-61 30.084555900871194 34.0 28.0 38.0 7.0 40.0 62-63 29.909305217916007 34.0 28.0 38.0 4.0 40.0 64-65 29.43303544981747 34.0 27.0 37.0 2.0 39.5 66-67 28.567962244754256 33.5 26.0 36.5 2.0 39.0 68-69 25.288484671331915 31.0 15.5 35.0 2.0 38.0 70-71 24.024032289475805 29.0 10.5 34.0 2.0 37.0 72-73 24.96787210637535 30.0 16.0 34.0 2.0 37.0 74-75 25.66380637240382 30.5 20.5 34.0 2.0 36.5 76-77 25.310047821939634 30.5 21.0 34.0 2.0 36.0 78-79 26.36109058570081 31.5 23.5 34.5 2.0 36.0 80-81 26.37938324916043 32.0 24.0 35.0 2.0 36.0 82-83 26.07934625021151 32.0 23.5 34.5 2.0 35.5 84-85 25.728518704689144 31.5 22.5 34.0 2.0 35.0 86-87 25.307117171733655 31.0 20.0 34.0 2.0 35.0 88-89 24.825507742287243 31.0 19.0 34.0 2.0 35.0 90-91 24.231147297320785 31.0 17.0 34.0 2.0 35.0 92-93 23.68537360247379 30.5 9.0 34.0 2.0 35.0 94-95 22.876442666350716 30.0 2.0 34.0 2.0 35.0 96-97 22.20696655089271 29.5 2.0 34.0 2.0 35.0 98-99 21.37741641625272 29.0 2.0 34.0 2.0 35.0 100-101 20.025884542704695 27.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2734898975151623 1101 2 0.26499063654327415 1101 3 0.39224417356251706 1101 4 0.29277943185353905 1101 5 0.5084654016309926 1101 6 0.3664384503251128 1101 7 0.5118620740427389 1101 8 0.6948714587739246 1101 9 0.6076458084037313 1101 10-11 0.7125051683823074 1101 12-13 0.746571588129413 1101 14-15 0.7252996692803535 1101 16-17 0.7433139002492624 1101 18-19 0.5501500581227603 1101 20-21 0.6889307146790316 1101 22-23 0.6492731471633135 1101 24-25 0.5962375102704485 1101 26-27 0.6356746358707355 1101 28-29 0.7165574167706836 1101 30-31 0.5742077559131928 1101 32-33 0.7719958745399893 1101 34-35 0.8139010139810736 1101 36-37 0.883872784955301 1101 38-39 0.9405160835475925 1101 40-41 0.9799725947148659 1101 42-43 0.6559961855550185 1101 44-45 0.9062027811910554 1101 46-47 0.7278730471651187 1101 48-49 0.6974720256921145 1101 50-51 0.5118066652819699 1101 52-53 0.39396196668556627 1101 54-55 0.5262906639384397 1101 56-57 0.5059916264278073 1101 58-59 0.5546082115792856 1101 60-61 0.6501118560501737 1101 62-63 0.3117064086390755 1101 64-65 0.2250372892861563 1101 66-67 0.1471131006644626 1101 68-69 -6.846139096599185 1101 70-71 -7.900491538687632 1101 72-73 -5.179477778093808 1101 74-75 -3.2282253738537143 1101 76-77 -0.488997057415439 1101 78-79 -0.19643114979512788 1101 80-81 -0.3167149250569423 1101 82-83 -0.529881561515726 1101 84-85 -0.5575875546414828 1101 86-87 -0.6142622685181323 1101 88-89 -0.680013908089105 1101 90-91 -0.8488551263111823 1101 92-93 -0.7841240558314091 1101 94-95 -0.7227314540128233 1101 96-97 -0.9296015100341251 1101 98-99 -1.2902680394117958 1101 100-101 -1.030576965971914 1102 1 0.3065287219834971 1102 2 0.32318696535412883 1102 3 0.2618240054952885 1102 4 0.2278622918131603 1102 5 0.516523239291196 1102 6 0.3760060762581716 1102 7 0.42940960702240716 1102 8 0.5257714165223888 1102 9 0.625836503676247 1102 10-11 0.5233347069016219 1102 12-13 0.5048927937066097 1102 14-15 0.616433418869569 1102 16-17 0.6788145809684778 1102 18-19 0.6002293971209269 1102 20-21 0.5237450426448547 1102 22-23 0.5988689407880301 1102 24-25 0.4613011223074466 1102 26-27 0.522586048480477 1102 28-29 0.4579338779696869 1102 30-31 0.40811414528458556 1102 32-33 0.5780462947080522 1102 34-35 0.3770886577536672 1102 36-37 0.4948347116254084 1102 38-39 0.48460415453017447 1102 40-41 0.40760885592326446 1102 42-43 0.41408829390673674 1102 44-45 0.42818203099152896 1102 46-47 0.3957365277528808 1102 48-49 0.46023245947456815 1102 50-51 0.284326265300745 1102 52-53 -0.008220090494845067 1102 54-55 -0.20681670191293122 1102 56-57 -0.02772134479420174 1102 58-59 -0.09828226304631826 1102 60-61 -0.022288500740589967 1102 62-63 -0.0350050133598927 1102 64-65 -0.2109234637301327 1102 66-67 -0.2631990912514084 1102 68-69 -6.9337565793464755 1102 70-71 -7.716901096924335 1102 72-73 -1.780781942217562 1102 74-75 -0.3805844180300433 1102 76-77 0.03931522839273782 1102 78-79 -0.2046786499124913 1102 80-81 -0.41539667708736516 1102 82-83 -0.28117117233211886 1102 84-85 -0.5281138210139567 1102 86-87 -0.4875452129808373 1102 88-89 -0.5562112697153196 1102 90-91 -0.7410658206447813 1102 92-93 -0.6004462942239677 1102 94-95 -0.3723206751761481 1102 96-97 -0.7532219973351921 1102 98-99 -0.8839537818651984 1102 100-101 -0.7372836933556037 1103 1 0.37988183507310325 1103 2 0.17091583548082312 1103 3 0.3643988432595755 1103 4 0.3124048556238179 1103 5 0.3879081861857756 1103 6 0.4557930145383793 1103 7 0.5199085304124083 1103 8 0.525735800628425 1103 9 0.5563445787562529 1103 10-11 0.5195783464079682 1103 12-13 0.5272359729386551 1103 14-15 0.6198298993600915 1103 16-17 0.6931897936671305 1103 18-19 0.6576414739708838 1103 20-21 0.5794070501501309 1103 22-23 0.6237242100213294 1103 24-25 0.6703191623424729 1103 26-27 0.7012687026789521 1103 28-29 0.6112659362185795 1103 30-31 0.6716678800469609 1103 32-33 0.901080911336976 1103 34-35 0.8515933655901122 1103 36-37 0.829398831025614 1103 38-39 0.9471318751246613 1103 40-41 0.9842281591211517 1103 42-43 0.5483754704944843 1103 44-45 0.7121879536489075 1103 46-47 0.8001597347291778 1103 48-49 0.8294552026574991 1103 50-51 0.6744522424387505 1103 52-53 0.6053468221482916 1103 54-55 0.4432978058440469 1103 56-57 0.6310117308785159 1103 58-59 0.6973447005456244 1103 60-61 0.5275489259929991 1103 62-63 0.28091030501483516 1103 64-65 0.35272814563516874 1103 66-67 0.15406268865235972 1103 68-69 -6.4989447753248974 1103 70-71 -7.412180013495217 1103 72-73 -1.0837213804198917 1103 74-75 0.32316236338288107 1103 76-77 0.5639360174644636 1103 78-79 0.37845767082532333 1103 80-81 0.22930352373312246 1103 82-83 0.18135686721067046 1103 84-85 -0.27901849129954925 1103 86-87 -0.10590023072339605 1103 88-89 -0.06235331036570457 1103 90-91 -0.22041615059148612 1103 92-93 -0.14141350282987375 1103 94-95 0.002565898833200464 1103 96-97 0.16517959749138456 1103 98-99 0.31158390809642 1103 100-101 0.2694068852198086 1104 1 0.2149649486755223 1104 2 0.3159199046235628 1104 3 0.16114966840293832 1104 4 0.2511746293107606 1104 5 0.4487358984307548 1104 6 0.3279636821795151 1104 7 0.4117400511403133 1104 8 0.5437715110228041 1104 9 0.5800343441288973 1104 10-11 0.4212831433028441 1104 12-13 0.39928118232977994 1104 14-15 0.524433698539724 1104 16-17 0.4838361713338628 1104 18-19 0.40797127987571 1104 20-21 0.5082975235787544 1104 22-23 0.49969298867505074 1104 24-25 0.44931159482655403 1104 26-27 0.6227544678035102 1104 28-29 0.5564045888001203 1104 30-31 0.5041748826602657 1104 32-33 0.699969869837588 1104 34-35 0.4113019588149669 1104 36-37 0.6777011582735355 1104 38-39 0.6335597440169636 1104 40-41 0.7899751527328931 1104 42-43 0.7163617100031345 1104 44-45 0.8110539263123115 1104 46-47 0.661757860157671 1104 48-49 0.5842207303553337 1104 50-51 0.5396936335407752 1104 52-53 0.3837615704648982 1104 54-55 0.33811649012383427 1104 56-57 0.47641076040263997 1104 58-59 0.6676512733635853 1104 60-61 0.6418592544914183 1104 62-63 0.3161813074068718 1104 64-65 0.29935233914029524 1104 66-67 0.22956204915577771 1104 68-69 -6.508925594618081 1104 70-71 -4.443010370054374 1104 72-73 0.16724697916660958 1104 74-75 0.7393196363783581 1104 76-77 1.016576863862916 1104 78-79 0.6369181666153771 1104 80-81 0.47728314826564855 1104 82-83 0.31453423070790265 1104 84-85 0.34818768809809697 1104 86-87 0.13910370222249568 1104 88-89 0.3468960061356512 1104 90-91 0.11572704593595518 1104 92-93 0.056017342696918604 1104 94-95 0.288728466743045 1104 96-97 0.13121351804964476 1104 98-99 0.057221837367933404 1104 100-101 0.26658630088923374 1105 1 0.1576380241987856 1105 2 0.2068167309657163 1105 3 0.14898500016135685 1105 4 0.18865395024327114 1105 5 0.3343455007970988 1105 6 0.23689306895417417 1105 7 0.2591587245036848 1105 8 0.3821145552462326 1105 9 0.41305873219393163 1105 10-11 0.4085160938252841 1105 12-13 0.23424960952954166 1105 14-15 0.3609348582218317 1105 16-17 0.3890011406626712 1105 18-19 0.49775896375397366 1105 20-21 0.5195448683864967 1105 22-23 0.5013684067230741 1105 24-25 0.44797200483456123 1105 26-27 0.537425604457404 1105 28-29 0.5063686899389523 1105 30-31 0.5474611338927389 1105 32-33 0.7000130890886425 1105 34-35 0.599083194132831 1105 36-37 0.7156950157633304 1105 38-39 0.5719446013148541 1105 40-41 0.6778195419228652 1105 42-43 0.6062828979411208 1105 44-45 0.5511914586543725 1105 46-47 0.5764777994108456 1105 48-49 0.7209139158581408 1105 50-51 0.5914401048646987 1105 52-53 0.5828335766322255 1105 54-55 0.45817605410982765 1105 56-57 0.675149626792706 1105 58-59 0.8930051335664722 1105 60-61 0.7700880840892843 1105 62-63 0.34773756439639314 1105 64-65 0.5122964841518396 1105 66-67 0.6175766270711911 1105 68-69 -2.7459486526054953 1105 70-71 -3.1471342724094704 1105 72-73 0.7510910854669426 1105 74-75 1.2750205988648737 1105 76-77 1.179628622741678 1105 78-79 0.8918499174210339 1105 80-81 0.6769190726034253 1105 82-83 0.7021827891287487 1105 84-85 0.7265881539561327 1105 86-87 0.515645326703865 1105 88-89 0.39872188507873574 1105 90-91 0.37404768232184793 1105 92-93 0.375420842587733 1105 94-95 0.4316878232766399 1105 96-97 0.4194236785853853 1105 98-99 0.4713085835745652 1105 100-101 0.4187372961313933 1106 1 0.10678648770016963 1106 2 0.17230520981263453 1106 3 0.07576552507136469 1106 4 0.23286341100579477 1106 5 0.24880469483369438 1106 6 0.23072443691677336 1106 7 0.3047301635987125 1106 8 0.36678909167284246 1106 9 0.291899771816702 1106 10-11 0.3853508743899212 1106 12-13 0.3218328656799585 1106 14-15 0.42827477507120904 1106 16-17 0.5021744632690286 1106 18-19 0.49271300164105725 1106 20-21 0.4870619334659594 1106 22-23 0.43118348226300895 1106 24-25 0.5191680668093284 1106 26-27 0.6001928994302048 1106 28-29 0.49508974220896107 1106 30-31 0.47598934476389587 1106 32-33 0.574967876043111 1106 34-35 0.6146524149951418 1106 36-37 0.6564984133012643 1106 38-39 0.6935075821561085 1106 40-41 0.5281073534790366 1106 42-43 0.5384065288238773 1106 44-45 0.6027140519339582 1106 46-47 0.5685303392459176 1106 48-49 0.707245194843118 1106 50-51 0.5236450571519988 1106 52-53 0.35958355945747655 1106 54-55 0.163647825856998 1106 56-57 0.24123651116070377 1106 58-59 0.5327339297862075 1106 60-61 0.7044717464742654 1106 62-63 0.2500272068222458 1106 64-65 0.3655686670997582 1106 66-67 0.4732687943093872 1106 68-69 -2.911950159844963 1106 70-71 -3.063402815965876 1106 72-73 0.6850679339045342 1106 74-75 1.144016575421137 1106 76-77 1.1833759254783018 1106 78-79 0.8050757368221291 1106 80-81 0.5654782269932497 1106 82-83 0.6104731442032438 1106 84-85 0.4012108681625044 1106 86-87 0.28617805018874165 1106 88-89 0.3706569992887516 1106 90-91 0.5663683551747383 1106 92-93 0.40825513563779126 1106 94-95 0.6140208619492071 1106 96-97 0.6701551573768434 1106 98-99 0.6806322584909061 1106 100-101 0.7142600044611065 1107 1 0.048337623639540084 1107 2 0.12636007111589365 1107 3 0.09699166342174692 1107 4 -0.2095970511548515 1107 5 0.20946245749062342 1107 6 0.08797132279556763 1107 7 0.07720714253704841 1107 8 0.13486660362394076 1107 9 0.34299282460355585 1107 10-11 0.13702802287177462 1107 12-13 0.15782819692005745 1107 14-15 0.1495001079375058 1107 16-17 0.013683089241624202 1107 18-19 0.17853524088246076 1107 20-21 0.10098035011936446 1107 22-23 0.20625036465428082 1107 24-25 0.15775214770083323 1107 26-27 0.5722212128488522 1107 28-29 0.3308998121097346 1107 30-31 0.30811454267895755 1107 32-33 0.24600061113398652 1107 34-35 0.09326120683214612 1107 36-37 0.3926129017611757 1107 38-39 0.5798802282649334 1107 40-41 0.3127720685520998 1107 42-43 0.13661103949121411 1107 44-45 0.35522748882351607 1107 46-47 0.42809740025872856 1107 48-49 0.38629298639080645 1107 50-51 0.46312735076140044 1107 52-53 0.20974730130984653 1107 54-55 0.11246263222188446 1107 56-57 0.4106569174686463 1107 58-59 0.5902417380836056 1107 60-61 0.5205389116557555 1107 62-63 0.34383959782337925 1107 64-65 0.061048388384847385 1107 66-67 0.3271889764968243 1107 68-69 -2.835420844904821 1107 70-71 -2.933932569785995 1107 72-73 0.951593092982197 1107 74-75 1.0865345643842623 1107 76-77 0.6503275121839813 1107 78-79 0.5471806866089004 1107 80-81 0.4571910148803333 1107 82-83 0.33824787904115183 1107 84-85 0.3126448997779754 1107 86-87 0.4591792142220932 1107 88-89 0.4868411120512981 1107 90-91 0.3479033836094132 1107 92-93 0.44401650640625334 1107 94-95 0.43587472275415706 1107 96-97 0.5288962162313879 1107 98-99 0.7288285075039163 1107 100-101 1.017482583007233 1108 1 0.10218974325863073 1108 2 0.07376510458098551 1108 3 0.0652777925767225 1108 4 0.06602065677292757 1108 5 0.12482294265933547 1108 6 -0.06693720610047649 1108 7 0.22249619647898555 1108 8 0.23168698021112988 1108 9 0.27638452456614004 1108 10-11 0.3771995232614387 1108 12-13 0.1853339718105147 1108 14-15 0.2314829616828007 1108 16-17 0.3082957180260735 1108 18-19 0.10908240243225009 1108 20-21 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2311 5 0.3497201127611902 2311 6 0.30688635698169975 2311 7 -2.762757730083294 2311 8 -0.775842861017253 2311 9 -3.123535501103042 2311 10-11 -1.3493378461957803 2311 12-13 -0.8325566568425486 2311 14-15 -1.7830043238497453 2311 16-17 0.038216678878967514 2311 18-19 0.39622290088517076 2311 20-21 -0.7105883922338094 2311 22-23 0.11943153655313665 2311 24-25 0.26803404396908803 2311 26-27 0.5662358141133197 2311 28-29 0.5019930898233582 2311 30-31 0.3723998405329283 2311 32-33 0.7258565136861534 2311 34-35 0.43372163322634094 2311 36-37 0.5863590349297425 2311 38-39 0.5995767199676081 2311 40-41 0.5456228158738909 2311 42-43 0.778091914357482 2311 44-45 0.8307777401283971 2311 46-47 0.8934122458129465 2311 48-49 1.1903592925209878 2311 50-51 0.7754774961447204 2311 52-53 1.0594512086110512 2311 54-55 1.431951585241265 2311 56-57 1.2655247048659994 2311 58-59 1.3138877579477146 2311 60-61 1.7467208646171066 2311 62-63 1.6200606992604314 2311 64-65 1.639318232807117 2311 66-67 1.9528279997627855 2311 68-69 4.11689988054885 2311 70-71 5.43891647756023 2311 72-73 4.132409195673798 2311 74-75 3.2696393681501412 2311 76-77 2.8183741830515494 2311 78-79 2.1866162102681237 2311 80-81 1.9072807267317522 2311 82-83 1.810555508689486 2311 84-85 1.8805416921910698 2311 86-87 1.834366136615209 2311 88-89 1.9947135790127923 2311 90-91 2.1952866471865384 2311 92-93 2.3647411155412783 2311 94-95 2.551127140519881 2311 96-97 2.4264619275063417 2311 98-99 2.6436606172090045 2311 100-101 2.94665374139619 2312 1 -0.14070092546961988 2312 2 -0.06043234195431424 2312 3 6.983670388258645E-4 2312 4 0.05274633510840232 2312 5 -1.4165777323654396 2312 6 -0.23595233565275464 2312 7 0.07639702637015944 2312 8 0.1477941317899507 2312 9 -2.71614774890287 2312 10-11 -0.02866173626904356 2312 12-13 -1.0217302486002993 2312 14-15 -3.923805636323401 2312 16-17 -0.8439305862449586 2312 18-19 -0.08053697863627463 2312 20-21 0.3146967402417289 2312 22-23 0.3944272320627533 2312 24-25 0.4829606182922248 2312 26-27 0.35372143913712506 2312 28-29 0.37886057318073085 2312 30-31 0.16926251566503936 2312 32-33 0.6898036690329334 2312 34-35 0.5114068181307303 2312 36-37 0.8273754539913369 2312 38-39 0.841461365690364 2312 40-41 0.5724921018945608 2312 42-43 0.20128936057325575 2312 44-45 0.6789400007153361 2312 46-47 0.8862513764777376 2312 48-49 1.1020918844374492 2312 50-51 1.1182888792726722 2312 52-53 1.0655883505170323 2312 54-55 1.1942833184160975 2312 56-57 1.0982000660978208 2312 58-59 1.1657397501939037 2312 60-61 1.2837961731505096 2312 62-63 1.3137491201981462 2312 64-65 1.4244008402736483 2312 66-67 1.8075241566531233 2312 68-69 4.2833005280250696 2312 70-71 5.045425237661462 2312 72-73 3.202230893742186 2312 74-75 2.4510619289361166 2312 76-77 2.433535053060659 2312 78-79 1.9001570924814253 2312 80-81 1.6948317193590299 2312 82-83 1.7739564739061713 2312 84-85 1.7651408321016646 2312 86-87 1.8332144537869084 2312 88-89 1.7430936249116336 2312 90-91 1.7439891339521232 2312 92-93 2.153123607671052 2312 94-95 2.3778306320596627 2312 96-97 2.3561668197323478 2312 98-99 2.346200873038935 2312 100-101 2.4719943857927227 2313 1 -0.34361683932552367 2313 2 -0.1379291777336782 2313 3 -0.14965766816856174 2313 4 0.02583355165702983 2313 5 -0.09310259685950228 2313 6 -0.12185681966740702 2313 7 -2.8625407616705374 2313 8 -3.0567980943721125 2313 9 -0.8042628395783211 2313 10-11 -0.12460042285177764 2313 12-13 0.03535374092637511 2313 14-15 -0.6055550880195213 2313 16-17 -0.17261340090375654 2313 18-19 0.19961007308302925 2313 20-21 -2.135371375042176 2313 22-23 -0.3476767089619699 2313 24-25 0.13792047375255834 2313 26-27 -1.3382018273047507 2313 28-29 -1.5196307457437257 2313 30-31 -0.7511295578415016 2313 32-33 -0.6887941222927552 2313 34-35 -1.2720445494889034 2313 36-37 -1.9873775435642358 2313 38-39 -1.8038053467085504 2313 40-41 -0.7955647284742255 2313 42-43 0.0036922359077919964 2313 44-45 0.1417495387874368 2313 46-47 0.06815783148783083 2313 48-49 -2.079336314323921 2313 50-51 -0.3372888446140543 2313 52-53 -0.35066608180728664 2313 54-55 -0.3330376736516101 2313 56-57 -0.32915329280692873 2313 58-59 -0.18195254263418903 2313 60-61 -0.04359438346247302 2313 62-63 0.33522234267908857 2313 64-65 0.24203303706401869 2313 66-67 0.8215699146651225 2313 68-69 1.6711301142126445 2313 70-71 4.086567352230404 2313 72-73 3.177733545890476 2313 74-75 2.0088633028358878 2313 76-77 1.9990730671861279 2313 78-79 1.4179830072068889 2313 80-81 1.2988524534582169 2313 82-83 1.2547682535025082 2313 84-85 1.0863478735795233 2313 86-87 1.097931702895611 2313 88-89 0.7081422717846095 2313 90-91 1.523580635483512 2313 92-93 1.5607211188062102 2313 94-95 1.7617206427841978 2313 96-97 1.9151541725115457 2313 98-99 1.8446453325127585 2313 100-101 1.7063423631069519 2314 1 -0.15010334418249371 2314 2 -0.12817079428822709 2314 3 -0.13594194685141758 2314 4 -0.02966035090553021 2314 5 -0.06882689426046795 2314 6 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2314 68-69 -1.3363979776577217 2314 70-71 1.556844999839761 2314 72-73 1.1426189530191735 2314 74-75 0.660524600463642 2314 76-77 0.8583387277543757 2314 78-79 -1.5256079354238032 2314 80-81 -0.9490028720205714 2314 82-83 0.3152650350202002 2314 84-85 0.3714404086220604 2314 86-87 0.05342554010164591 2314 88-89 0.19529726283403193 2314 90-91 0.4086237283113512 2314 92-93 0.01398303052391725 2314 94-95 0.41166616811671375 2314 96-97 0.5513952505282909 2314 98-99 0.4436217512232865 2314 100-101 0.822280108398175 2315 1 -0.30298632819524585 2315 2 -0.2685766520073649 2315 3 -0.330110881667121 2315 4 -0.10153376512337076 2315 5 -0.21318215047160294 2315 6 -0.11531903254224574 2315 7 -0.9613116482763786 2315 8 -2.954326081877557 2315 9 -0.6715071609601182 2315 10-11 -1.335016280448798 2315 12-13 -0.501110881084692 2315 14-15 -0.3626199262308134 2315 16-17 -0.3014564607253192 2315 18-19 -0.0820483810013215 2315 20-21 -0.544264500513961 2315 22-23 -0.1980002527626965 2315 24-25 -5.092893437706223 2315 26-27 -0.9971354693034087 2315 28-29 -0.7072189868725332 2315 30-31 -1.0647644619047512 2315 32-33 -0.06681743694813491 2315 34-35 -2.02836806975305 2315 36-37 -3.4143249847186112 2315 38-39 -1.1406924501803601 2315 40-41 -0.2630001163821518 2315 42-43 -1.5717497052541418 2315 44-45 -5.052595379582691 2315 46-47 -3.46668890340127 2315 48-49 -2.3982931734693054 2315 50-51 -0.9855081348519228 2315 52-53 -0.21313138637892948 2315 54-55 -1.2573634769632065 2315 56-57 -1.0506660555128953 2315 58-59 -0.4539441905136279 2315 60-61 -1.4671194389835627 2315 62-63 -0.3876012389482639 2315 64-65 0.2323801006245283 2315 66-67 0.43753855386460927 2315 68-69 3.0979156573778788 2315 70-71 3.665510610599757 2315 72-73 -1.9259788439289487 2315 74-75 -1.4843675791635107 2315 76-77 0.024870074342924653 2315 78-79 -0.002330062819456913 2315 80-81 -0.15646546678306805 2315 82-83 -0.6807396909310093 2315 84-85 -0.26458753702437576 2315 86-87 0.1849361529888327 2315 88-89 0.2669120824328921 2315 90-91 0.5359353181684945 2315 92-93 -0.7329558652847474 2315 94-95 0.3027094141583433 2315 96-97 0.06239820809211949 2315 98-99 -2.208663825399146 2315 100-101 -3.6383827549541756 2316 1 -0.5740030996837149 2316 2 -0.45584359076466896 2316 3 -0.8960817099823899 2316 4 -0.4533921218743373 2316 5 -0.17222191584918534 2316 6 -0.5662780405265408 2316 7 -0.5517907062183056 2316 8 -0.800546921344079 2316 9 -5.051281571058695 2316 10-11 -1.4960415268915455 2316 12-13 -0.7582055886987646 2316 14-15 -0.3751754037275745 2316 16-17 -0.3438415470360354 2316 18-19 -0.545535545906084 2316 20-21 -3.6261177834436253 2316 22-23 -1.0904489012305092 2316 24-25 -0.35097112850421297 2316 26-27 -0.5933359577284065 2316 28-29 0.10027493569315027 2316 30-31 0.15647226810104797 2316 32-33 -1.2085228542281143 2316 34-35 -0.43048206903936403 2316 36-37 -0.04750944878926333 2316 38-39 -5.198844575609687 2316 40-41 -4.8270886127468415 2316 42-43 -3.4740596878581833 2316 44-45 -2.0600589149767785 2316 46-47 -2.2590107063362836 2316 48-49 -2.6760386126057583 2316 50-51 -1.1255444879452803 2316 52-53 -0.6378032606819772 2316 54-55 0.06587183683312503 2316 56-57 -3.6131535103503083 2316 58-59 -0.5398609909953613 2316 60-61 -0.29013786085786464 2316 62-63 0.09940531799004759 2316 64-65 0.32059907783136055 2316 66-67 0.16235037642583094 2316 68-69 2.8788118837929737 2316 70-71 4.045937926715553 2316 72-73 2.8084765845022943 2316 74-75 1.4816590054299148 2316 76-77 -0.9326110242787813 2316 78-79 0.42621464256437136 2316 80-81 0.5977287992412883 2316 82-83 0.7235655300868764 2316 84-85 0.8696353877269551 2316 86-87 1.0604326626458231 2316 88-89 0.7308910177110022 2316 90-91 0.7801762521976698 2316 92-93 0.6526152462724149 2316 94-95 1.0208073444852985 2316 96-97 1.0974555255460636 2316 98-99 0.9352523283097618 2316 100-101 0.9431184270789217 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12.0 3 45.0 4 147.0 5 383.0 6 977.0 7 2184.0 8 4276.0 9 6909.0 10 10581.0 11 15217.0 12 19274.0 13 24082.0 14 28726.0 15 35398.0 16 41722.0 17 46070.0 18 41666.0 19 36364.0 20 35613.0 21 36394.0 22 37968.0 23 42302.0 24 49218.0 25 59723.0 26 74058.0 27 90624.0 28 109552.0 29 128937.0 30 149671.0 31 172419.0 32 198377.0 33 227327.0 34 259876.0 35 284093.0 36 269987.0 37 201402.0 38 73757.0 39 3668.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.83881505534825 19.39624478476824 15.124429335938098 21.640510823945412 2 16.48140638155423 21.852624139292743 37.25933282170769 24.40663665744534 3 20.273645077297463 25.583686297880792 29.945121994480274 24.19754663034147 4 13.317279235141289 17.26947059468929 35.42059097876619 33.99265919140323 5 13.63810345445316 38.59100340227152 32.245772346850785 15.525120796424547 6 31.752157414741898 35.79770691653314 16.42221228173547 16.027923386989496 7 28.31547652198529 34.28653220522604 19.202241646768943 18.195749626019733 8 27.6805348281429 32.89994072363985 18.685817199651368 20.733707248565892 9 26.993659806193616 16.951158904277726 19.936970534576282 36.11821075495238 10-11 25.88391084037497 26.58369557794017 25.287668486980923 22.24472509470394 12-13 27.491797620360988 24.22457404206245 26.604337213315794 21.679291124260775 14-15 23.735872201444554 25.28938463617759 26.441282880909146 24.533460281468706 16-17 22.014144817474953 28.763174520740282 26.68017153704013 22.542509124744633 18-19 21.683385970258215 26.60210351412222 30.221369515469508 21.493141000150054 20-21 23.077872677500064 25.147383876333407 30.008080882611164 21.76666256355536 22-23 22.414771381893132 25.298834707376734 30.234677898106465 22.05171601262366 24-25 22.5147012575688 25.67860451528553 30.241107186991766 21.565587040153904 26-27 22.45849008746304 25.608880043846817 30.584287161149454 21.348342707540695 28-29 21.708363685769235 26.241070974060882 30.57844071577296 21.472124624396923 30-31 21.738939185585497 25.970164184428175 30.314124415638272 21.97677221434806 32-33 21.762520798750042 26.04946222560327 30.137430939104025 22.050586036542658 34-35 22.58337556291575 25.79938071950695 29.921567382361275 21.695676335216028 36-37 22.562182629604283 25.522451251108386 30.187894812988343 21.72747130629899 38-39 22.293789060162837 25.809086094497697 30.152062220361593 21.745062624977873 40-41 22.007116568511794 25.994965221259392 30.300922285410397 21.69699592481842 42-43 21.997783581640473 26.318450289371647 29.35541917484126 22.328346954146625 44-45 22.084484410992903 26.199536379675187 29.23105024257614 22.48492896675577 46-47 22.501618867474395 26.609353077836285 28.568129469636293 22.320898585053026 48-49 22.545841169146172 26.530146764515592 28.437473097905432 22.486538968432804 50-51 22.190314008066927 26.906807188240833 28.586594503101598 22.31628430059064 52-53 22.656063797522087 27.061860106796537 27.817659708097516 22.46441638758386 54-55 21.87446043312281 26.664740714214858 28.30323174939715 23.15756710326518 56-57 22.551143730435445 27.04936850524123 27.731001045335486 22.66848671898785 58-59 22.883933177733574 26.480427144753904 27.692043761899725 22.943595915612796 60-61 22.508621697425564 26.530010922028918 28.622350228330184 22.339017152215334 62-63 22.58472471109249 26.593786987548683 28.22507479322447 22.596413508134354 64-65 22.209135568372197 27.000116183470553 28.16723679344264 22.62351145471461 66-67 22.18162065105925 26.71399233644604 28.21405370953706 22.89033330295765 68-69 22.050385979553518 26.49108002823753 28.793953225165698 22.66458076704326 70-71 21.72676105726053 26.613048658744727 28.953115998812066 22.707074285182678 72-73 22.232917481937957 26.479524935865523 28.228371957005294 23.05918562519123 74-75 22.011476455697103 27.4616305369196 27.60807202820223 22.918820979181067 76-77 21.61212206726114 26.95702070498931 28.11836103730907 23.31249619044048 78-79 22.079616998834343 26.9708539561478 27.87900580487029 23.07052324014757 80-81 22.00951005547887 27.549379884643393 27.62279476010417 22.818315299773566 82-83 22.10779620347867 27.109855481599705 27.530615133512526 23.251733181409097 84-85 21.892331094000202 27.07135949916885 27.31614540506294 23.72016400176801 86-87 22.152980363033173 26.93459446775006 27.461050567349076 23.451374601867688 88-89 21.97022302842099 26.90299949574147 27.062489213780605 24.064288262056934 90-91 21.814205707867767 26.901685456037345 27.406371535371658 23.877737300723233 92-93 21.90559844306572 26.98601533307815 27.577475111507326 23.5309111123488 94-95 21.624461639172928 26.692419709153803 27.649061871202363 24.0340567804709 96-97 21.299212766078252 26.53044676163817 27.737486728025303 24.432853744258274 98-99 21.561135971520887 26.71563140493581 27.027166063477004 24.696066560066303 100-101 21.47077924439305 26.21432143336734 27.57298204104382 24.74191728119579 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10488.0 1 6982.5 2 3701.0 3 4148.0 4 3926.5 5 3752.5 6 5589.5 7 10020.0 8 14054.5 9 15080.5 10 12900.5 11 9885.0 12 8564.5 13 7950.0 14 7599.5 15 7216.5 16 7015.5 17 7116.0 18 7138.5 19 7316.5 20 7735.0 21 8436.0 22 9401.5 23 10517.0 24 11783.0 25 13370.5 26 15414.0 27 17949.0 28 20772.5 29 23911.0 30 27226.0 31 30972.5 32 35029.0 33 37827.0 34 40261.5 35 43179.5 36 46027.5 37 48692.5 38 51243.0 39 53049.5 40 55696.0 41 61390.5 42 67360.0 43 71468.5 44 74904.5 45 80132.5 46 86977.5 47 101002.5 48 127704.5 49 130385.0 50 111303.0 51 102914.5 52 100400.5 53 101870.5 54 102406.5 55 98870.0 56 92455.5 57 85596.5 58 79687.5 59 73196.5 60 64773.0 61 54288.0 62 43623.0 63 33970.5 64 26338.5 65 20994.5 66 16197.5 67 12493.0 68 8915.0 69 5827.0 70 4125.5 71 3119.0 72 2613.5 73 2221.0 74 1797.0 75 1405.0 76 1015.0 77 619.5 78 354.5 79 226.0 80 143.5 81 90.0 82 55.5 83 35.0 84 18.5 85 8.0 86 4.0 87 2.5 88 2.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020290890489851186 2 0.0188719470989525 3 6.73998110676875E-4 4 0.09535299586839158 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.012291597123659853 12-13 0.0 14-15 0.0 16-17 5.675773563594737E-4 18-19 7.094716954493421E-5 20-21 0.0 22-23 2.660518857935033E-4 24-25 0.0038134103630402135 26-27 0.0033345169686119077 28-29 0.0011528915051051808 30-31 1.5963113147610198E-4 32-33 6.917349030631085E-4 34-35 0.002926570743728536 36-37 0.08501244590721742 38-39 0.09757009491667078 40-41 0.0777580978212479 42-43 0.08132319309088085 44-45 0.07997519686952709 46-47 0.07857399027101464 48-49 0.07348353085616562 50-51 0.07325295255514458 52-53 0.056243368656746597 54-55 0.04772970831135449 56-57 0.02713729235093734 58-59 0.02330614519551089 60-61 0.013604119760241134 62-63 0.00214615187873426 64-65 0.006438455636202781 66-67 0.15067405132105405 68-69 0.7054631803700534 70-71 0.8594894854521055 72-73 0.3498582298184568 74-75 0.05290885168813469 76-77 0.18990783607940265 78-79 0.014810221642505016 80-81 0.003121675459977106 82-83 0.0017736792386233552 84-85 1.7736792386233554E-4 86-87 0.0019687839548719246 88-89 3.015254705659704E-4 90-91 0.004185883003151119 92-93 0.004469671681330855 94-95 0.07502663179376794 96-97 0.03657326590041359 98-99 0.013196173535357764 100-101 0.01339127825160633 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2818999.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.4841518673222 #Duplication Level Percentage of deduplicated Percentage of total 1 65.25677669324634 23.155813745550848 2 14.381191094139629 10.206087376348643 3 6.6301793439143735 7.057988722411208 4 3.815965025271887 5.416251299085506 5 2.4170682057623383 4.28838076434734 6 1.5843289469100637 3.3731141375970815 7 1.1720514337508787 2.9112475750070232 8 0.834918117464974 2.3701089021524715 9 0.6215569440553871 1.984987889734504 >10 2.98923071125348 18.042571863259774 >50 0.16796580951151657 4.146816696960719 >100 0.11424579394749841 7.801411269442323 >500 0.009306275705756243 2.270208219441182 >1k 0.0043974709378830435 3.257981041262269 >5k 4.0906706398912026E-4 0.9725679426026963 >10k+ 4.0906706398912026E-4 2.7444625547964376 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25527 0.9055341984867679 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20280 0.7194042991856329 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16307 0.5784677468846211 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13515 0.47942549819989294 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9882 0.35054996472151995 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6314 0.2239802142533573 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5573 0.19769428793695917 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5032 0.17850307857505449 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4834 0.171479308790106 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4635 0.16442006542038504 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4469 0.1585314503481555 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4304 0.15267830886069841 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4010 0.1422490749375931 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3059 0.10851369581897688 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3022 0.1072011731823956 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2934 0.1040794977224185 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2922 0.10365381470514888 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 3.547358477246711E-5 3 0.0 0.0 0.0 0.0 3.547358477246711E-5 4 0.0 0.0 0.0 0.0 3.547358477246711E-5 5 0.0 0.0 0.0 0.0 3.547358477246711E-5 6 0.0 0.0 0.0 0.0 3.547358477246711E-5 7 0.0 0.0 0.0 0.0 3.547358477246711E-5 8 0.0 0.0 0.0 0.0 3.547358477246711E-5 9 0.0 0.0 0.0 0.0 7.094716954493421E-5 10-11 0.0 0.0 0.0 0.0 7.094716954493421E-5 12-13 0.0 0.0 0.0 1.7736792386233554E-5 7.094716954493421E-5 14-15 0.0 0.0 0.0 5.321037715870066E-5 3.1926226295220396E-4 16-17 0.0 0.0 0.0 1.0642075431740132E-4 3.5473584772467107E-4 18-19 0.0 0.0 0.0 1.5963113147610198E-4 3.5473584772467107E-4 20-21 0.0 0.0 0.0 4.0794622488337176E-4 4.0794622488337176E-4 22-23 0.0 0.0 0.0 0.0013657330137399836 4.966301868145395E-4 24-25 0.0 0.0 0.0 0.0025540981036176317 4.966301868145395E-4 26-27 0.0 0.0 0.0 0.0037956735706539805 5.321037715870066E-4 28-29 0.0 0.0 0.0 0.006952822615403554 5.321037715870066E-4 30-31 0.0 0.0 0.0 0.015005326358753585 5.321037715870066E-4 32-33 0.0 0.0 0.0 0.02836113102558745 5.321037715870066E-4 34-35 0.0 0.0 0.0 0.04755234038749216 5.321037715870066E-4 36-37 0.0 0.0 0.0 0.07422847613638742 5.498405639732401E-4 38-39 0.0 0.0 0.0 0.1163533580536921 5.675773563594737E-4 40-41 0.0 0.0 0.0 0.16147575788427027 5.675773563594737E-4 42-43 0.0 0.0 0.0 0.20587094922701285 5.675773563594737E-4 44-45 0.0 0.0 0.0 0.25853148582174024 5.675773563594737E-4 46-47 0.0 0.0 0.0 0.32390930255739714 5.675773563594737E-4 48-49 0.0 0.0 0.0 0.3970913079429968 5.675773563594737E-4 50-51 0.0 0.0 0.0 0.4784145010338776 5.675773563594737E-4 52-53 0.0 0.0 0.0 0.553246028111397 6.030509411319408E-4 54-55 0.0 0.0 0.0 0.63625421647897 6.030509411319408E-4 56-57 0.0 0.0 0.0 0.7359882000667612 6.030509411319408E-4 58-59 0.0 0.0 0.0 0.8347821336580822 6.030509411319408E-4 60-61 0.0 0.0 0.0 0.9425863577816097 6.030509411319408E-4 62-63 0.0 0.0 0.0 1.0468786970126631 6.030509411319408E-4 64-65 0.0 0.0 0.0 1.1546474475514181 6.030509411319408E-4 66-67 0.0 0.0 0.0 1.2742821121965635 6.030509411319408E-4 68-69 0.0 0.0 0.0 1.4084964201831927 6.030509411319408E-4 70-71 0.0 0.0 0.0 1.5781665761499029 6.030509411319408E-4 72-73 0.0 0.0 0.0 1.764402896205355 6.030509411319408E-4 74-75 0.0 0.0 0.0 1.9672763275190945 6.030509411319408E-4 76-77 0.0 0.0 0.0 2.1930124842186887 6.030509411319408E-4 78-79 0.0 0.0 0.0 2.450071816272372 6.030509411319408E-4 80-81 0.0 0.0 0.0 2.735634173690732 6.030509411319408E-4 82-83 0.0 0.0 0.0 3.0264643584478037 6.030509411319408E-4 84-85 0.0 0.0 0.0 3.3359004384180344 6.562613182906415E-4 86-87 0.0 0.0 0.0 3.662807258888705 6.73998110676875E-4 88-89 0.0 0.0 0.0 4.00221851799167 6.73998110676875E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 19425 0.0 24.604834 1 TACATGG 19325 0.0 24.338327 2 ACGCATA 405 0.0 23.08562 64-65 ACATGGG 19475 0.0 22.978651 3 CGCATAG 430 0.0 22.30276 66-67 AGTACTT 18070 0.0 22.093998 12-13 GAGTACT 17880 0.0 21.745367 12-13 CATGGGA 12585 0.0 21.53718 4 ATGGGAG 4495 0.0 20.890846 5 GTACTTT 18925 0.0 20.532103 14-15 GTATCAA 38080 0.0 19.917362 1 TACTTTT 20160 0.0 19.768219 14-15 ATAACGC 305 4.623871E-9 18.671179 3 GGACCGT 305 4.658432E-9 18.659584 6 ACTTTTT 21830 0.0 18.45094 16-17 CATGGGG 7225 0.0 18.396116 4 AACGCAT 555 0.0 18.14213 64-65 CAATTCG 610 0.0 17.880991 78-79 AGGTAAG 855 0.0 17.763166 2 ATTCGCG 615 0.0 17.733887 80-81 >>END_MODULE