FastQCFastQC Report
Fri 27 May 2016
SRR939389_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939389_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2917467
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95490.32730447336679386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74160.2541931065544186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50280.1723412809810702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA150600.032.861451
GGTATCA100500.031.7311331
TATCAAC183900.026.9407862
ATCAACG188650.026.1173533
TCAACGC190800.026.054674
GTACATG115300.025.9185961
CAACGCA190850.025.7828085
AACGCAG194550.025.3126586
TACATGG117200.024.6384772
ACATGGG120750.023.0215053
GAGTACT104700.022.74097412-13
ACGCAGA213300.022.5770787
CGCAGAG214650.022.29948
AGTACTT109650.021.2119212-13
GCAGAGT211850.020.862369
GTACTTT116050.020.68948214-15
AGAGTAC169000.020.29705410-11
CATGGGA69750.019.9809154
ACTTTTT145150.018.04802716-17
TACTTTT132800.017.65214-15
Produced by FastQC (version 0.11.5)