FastQCFastQC Report
Fri 27 May 2016
SRR939389_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939389_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2917467
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134630.4614619462705148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122290.41916498112917816No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82700.28346507432646195No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33720.1155797134980447No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33630.11527122671824565No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32000.10968418837299616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110000.033.478921
GTATCAA157700.030.1447981
ATCAACG196850.024.06453
TATCAAC197650.024.0418872
TCAACGC199000.023.8283144
CAACGCA201500.023.485665
AACGCAG206200.022.9503426
GTACATG127750.022.7798351
TACATGG128150.021.516362
GAGTACT122950.021.19221112-13
ACATGGG129500.020.6310673
ACGCAGA233800.020.1802797
CGCAGAG234050.020.0980058
GTACTTT130000.019.91539414-15
AGTACTT125650.019.62457812-13
AGAGTAC192600.019.35700610-11
GCAGAGT243700.018.6607049
CATGGGA73450.017.7358994
GTGTAGC16350.017.6829681
ACTTTTT153600.017.4721816-17
Produced by FastQC (version 0.11.5)