##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939389_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2917467 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.998178214183742 31.0 31.0 31.0 30.0 34.0 2 31.253973052651496 31.0 31.0 34.0 30.0 34.0 3 31.248576590583543 31.0 31.0 34.0 28.0 34.0 4 35.177472101655304 35.0 35.0 37.0 33.0 37.0 5 34.791965084780735 35.0 35.0 37.0 32.0 37.0 6 34.74824770939997 35.0 35.0 37.0 32.0 37.0 7 34.618183856064185 35.0 35.0 37.0 30.0 37.0 8 34.63820464807314 35.0 35.0 37.0 30.0 37.0 9 35.99922021397329 37.0 35.0 39.0 30.0 39.0 10-11 35.850117584877566 37.0 35.0 39.0 30.0 39.0 12-13 35.809288674044986 37.0 35.0 39.0 30.0 39.0 14-15 36.68092663944442 38.0 36.0 40.0 31.0 41.0 16-17 36.68660365310045 38.0 35.5 40.0 31.0 41.0 18-19 36.642614466590366 38.0 35.0 40.0 30.0 41.0 20-21 36.53735723488903 38.0 35.0 40.0 30.0 41.0 22-23 36.3988015974131 38.0 35.0 40.0 30.0 41.0 24-25 36.16998032882634 38.0 34.0 40.0 29.5 41.0 26-27 35.794438463228545 38.0 34.0 40.0 27.5 41.0 28-29 35.51590986290505 38.0 34.0 40.0 27.0 41.0 30-31 35.30652771736578 38.0 34.0 40.0 27.0 41.0 32-33 35.09861962448933 37.5 33.0 40.0 27.0 41.0 34-35 35.056902614494014 38.0 33.0 40.0 26.5 41.0 36-37 35.057531756143256 38.0 33.0 40.0 26.0 41.0 38-39 35.0282980064556 38.0 33.0 40.0 26.0 41.0 40-41 34.81985640283163 38.0 33.0 40.0 25.5 41.0 42-43 34.518778961338725 38.0 33.0 40.0 24.5 41.0 44-45 34.342042086508606 37.5 33.0 40.0 24.0 41.0 46-47 34.030953734866586 37.0 33.0 40.0 23.5 41.0 48-49 33.72500802922535 37.0 32.5 40.0 23.0 41.0 50-51 33.462248587559 37.0 32.0 40.0 22.5 41.0 52-53 33.08840991174878 37.0 31.0 40.0 20.5 41.0 54-55 32.86668006870343 36.5 31.0 39.0 19.5 41.0 56-57 32.46734735988445 36.0 31.0 39.0 18.5 40.0 58-59 32.0340754496966 35.5 30.0 39.0 16.5 40.0 60-61 31.739311875678457 35.0 30.0 39.0 14.5 40.0 62-63 31.35128143694513 35.0 29.0 38.0 13.0 40.0 64-65 30.92010518028139 34.5 29.0 38.0 9.5 40.0 66-67 29.230984446439326 33.5 25.5 37.5 7.0 39.5 68-69 25.584322290534907 30.0 16.0 35.0 4.0 39.0 70-71 24.971207900552088 29.5 15.0 35.0 2.0 38.0 72-73 26.79782599083383 31.0 21.5 35.0 2.0 38.0 74-75 27.404095916080628 32.0 24.5 35.0 2.0 38.0 76-77 27.449372520751737 31.5 25.5 34.5 2.0 36.5 78-79 28.282562407732463 33.0 26.0 35.0 2.0 37.0 80-81 28.139191291623863 33.0 26.0 35.0 2.0 37.0 82-83 27.734664865103873 33.0 26.0 35.0 2.0 36.0 84-85 27.40648308961164 32.5 26.0 35.0 2.0 36.0 86-87 26.957984100591368 32.0 25.5 35.0 2.0 35.5 88-89 26.47725372729152 32.0 25.0 34.0 2.0 35.0 90-91 26.032597969402907 32.0 24.0 34.0 2.0 35.0 92-93 25.477668470628803 31.5 21.5 34.0 2.0 35.0 94-95 24.111136646961214 31.0 15.0 34.0 2.0 35.0 96-97 23.315768438854665 30.0 4.5 34.0 2.0 35.0 98-99 22.976102557458233 30.5 2.0 34.0 2.0 35.0 100-101 21.710337769030463 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.37604206665556106 1101 2 0.2517784367443312 1101 3 0.3701991344844693 1101 4 0.22383872550389583 1101 5 0.43182924038311654 1101 6 0.4914897759851442 1101 7 0.5646619439092149 1101 8 0.41646525758577013 1101 9 0.6165752548915009 1101 10-11 0.7843746743413504 1101 12-13 0.6288867653028163 1101 14-15 0.7974874441728304 1101 16-17 0.6583680514072441 1101 18-19 0.7885246894064224 1101 20-21 0.8471209268571016 1101 22-23 0.7605559430923918 1101 24-25 0.7828817786372753 1101 26-27 0.8488427435577606 1101 28-29 0.7430829498449256 1101 30-31 0.8164407965581546 1101 32-33 0.6980685081778546 1101 34-35 0.8060174418572785 1101 36-37 0.7629559432539423 1101 38-39 0.6911076114358963 1101 40-41 0.9521525845126817 1101 42-43 0.9204905983010647 1101 44-45 0.5620800028450716 1101 46-47 0.7340495835997842 1101 48-49 0.6759954659261922 1101 50-51 0.5010810073044141 1101 52-53 0.6722417556898392 1101 54-55 0.5438560768276233 1101 56-57 0.6699804367699542 1101 58-59 0.6904573116105972 1101 60-61 0.3439633157434976 1101 62-63 0.3670609096095916 1101 64-65 0.23640394391773256 1101 66-67 -3.1132857817210216 1101 68-69 -7.876189544181685 1101 70-71 -11.151887398375 1101 72-73 -11.192423436522134 1101 74-75 -9.72540090031912 1101 76-77 -4.684850706406554 1101 78-79 -1.4484660626917538 1101 80-81 -1.0011403851132563 1101 82-83 -0.6574095562873623 1101 84-85 -0.7140060395523804 1101 86-87 -0.552519748138085 1101 88-89 -0.5687206838291523 1101 90-91 -0.6424427674895412 1101 92-93 -1.325910508382016 1101 94-95 -4.895861161273764 1101 96-97 -1.9096210767331243 1101 98-99 -1.2017798739392411 1101 100-101 -0.7358806854143722 1102 1 0.366401816599943 1102 2 0.2025060142106625 1102 3 0.3652742241299656 1102 4 0.19532911012409215 1102 5 0.44736328975952233 1102 6 0.4650900787116541 1102 7 0.47240231689494294 1102 8 0.45746194118522254 1102 9 0.5708395122762937 1102 10-11 0.5658517311086797 1102 12-13 0.5623782574248537 1102 14-15 0.5015717300067877 1102 16-17 0.5792782986215528 1102 18-19 0.6220624892368178 1102 20-21 0.8164273831044255 1102 22-23 0.6724362872566516 1102 24-25 0.647325695318635 1102 26-27 0.7358678481003835 1102 28-29 0.6127650820051471 1102 30-31 0.2775688431264811 1102 32-33 0.46556312904763075 1102 34-35 0.7298253486357709 1102 36-37 0.9397545103271057 1102 38-39 0.7933597253322375 1102 40-41 0.7293230344100223 1102 42-43 0.9984549847009063 1102 44-45 0.750723014849747 1102 46-47 0.7227477805415532 1102 48-49 0.5868462717373575 1102 50-51 0.5244539899241616 1102 52-53 0.538686651768721 1102 54-55 0.4526029193815191 1102 56-57 0.6695887678339361 1102 58-59 0.5563384486204619 1102 60-61 0.35624761885151557 1102 62-63 0.5015823003780966 1102 64-65 0.40469638445205547 1102 66-67 -2.5198604054371145 1102 68-69 -7.410259837621631 1102 70-71 -11.080266527499848 1102 72-73 -9.303120727326036 1102 74-75 -7.851402703587521 1102 76-77 -4.005706279099396 1102 78-79 -0.9773278648572585 1102 80-81 -0.3525166107738116 1102 82-83 -0.33952084951343764 1102 84-85 -0.3327763542196145 1102 86-87 -0.25450597552304544 1102 88-89 -0.16627136935237274 1102 90-91 -0.18296741671140282 1102 92-93 -0.6537353561555577 1102 94-95 -4.225183928803364 1102 96-97 -1.2795091265721084 1102 98-99 -0.5014043081159905 1102 100-101 -0.2339808383689217 1103 1 0.34781301016694144 1103 2 0.30184615348227695 1103 3 0.35421676800738666 1103 4 0.28031135586683575 1103 5 0.42936216762854684 1103 6 0.458140300234426 1103 7 0.48467556278644963 1103 8 0.40658411671935824 1103 9 0.7423490781421336 1103 10-11 0.7980546619191813 1103 12-13 0.6480362760325917 1103 14-15 0.7804115163819176 1103 16-17 0.8184892735396261 1103 18-19 0.8233602353822462 1103 20-21 0.8507983375989667 1103 22-23 0.7961555155357658 1103 24-25 0.9520533646214488 1103 26-27 0.9152491893554711 1103 28-29 0.9692802491599721 1103 30-31 1.0367360429088706 1103 32-33 0.963305804165671 1103 34-35 1.0757411004778135 1103 36-37 0.9204512351578558 1103 38-39 0.9157881382168895 1103 40-41 1.1137145100398627 1103 42-43 1.0442191867511283 1103 44-45 0.7187867947156903 1103 46-47 0.8827607291166615 1103 48-49 0.7786570226038734 1103 50-51 0.7738242331047971 1103 52-53 0.776468590626088 1103 54-55 0.7263750747395719 1103 56-57 0.6437773893549021 1103 58-59 0.7638580965107131 1103 60-61 0.6415395597289653 1103 62-63 0.6260171311578482 1103 64-65 0.4688011478300105 1103 66-67 -2.8584553913789286 1103 68-69 -7.5195619701535605 1103 70-71 -11.742643325514596 1103 72-73 -9.461280858432577 1103 74-75 -7.71798683220727 1103 76-77 -3.8707466149854746 1103 78-79 -1.1254931728305486 1103 80-81 -0.45306213875851853 1103 82-83 -0.09645502640690395 1103 84-85 -0.19241809847766334 1103 86-87 0.030348677362173504 1103 88-89 0.04714252237667793 1103 90-91 0.006243168783253594 1103 92-93 0.11002211054553968 1103 94-95 0.9349016792803155 1103 96-97 1.0680820825540849 1103 98-99 0.6677095643193454 1103 100-101 0.7380171531921249 1104 1 0.4000702785132795 1104 2 0.24056679077832044 1104 3 0.368895957265984 1104 4 0.17847509396627714 1104 5 0.46735169830935064 1104 6 0.4871102637821281 1104 7 0.622880821240841 1104 8 0.4110006359807912 1104 9 0.6397330702743034 1104 10-11 0.7304949518657082 1104 12-13 0.640140643407058 1104 14-15 0.6502889051679972 1104 16-17 0.7052768562357556 1104 18-19 0.7323236300901996 1104 20-21 0.7018548535322395 1104 22-23 0.6440455649391197 1104 24-25 0.8447532918956995 1104 26-27 0.9498645836909603 1104 28-29 0.9120239848993776 1104 30-31 0.880764882039955 1104 32-33 0.7152099706452262 1104 34-35 0.9164825065050195 1104 36-37 1.0224163369087762 1104 38-39 0.9178288720218006 1104 40-41 1.0184804764126127 1104 42-43 1.190428262480964 1104 44-45 0.9058309587418947 1104 46-47 0.7827402294377137 1104 48-49 0.8580142055494662 1104 50-51 0.805731268956194 1104 52-53 0.9372976116395719 1104 54-55 0.7589400151758099 1104 56-57 0.8033511573863947 1104 58-59 0.8957599709920281 1104 60-61 0.7879861459637887 1104 62-63 0.8384545070200886 1104 64-65 0.6597514289642987 1104 66-67 -2.5972901620226096 1104 68-69 -7.286450148049951 1104 70-71 -8.875646340882259 1104 72-73 -8.300765990867891 1104 74-75 -7.198328767629686 1104 76-77 -3.5665136402653026 1104 78-79 -0.5434465855848281 1104 80-81 0.20320193715308577 1104 82-83 0.4235338129807822 1104 84-85 0.5079295698563868 1104 86-87 0.6316874648348367 1104 88-89 0.6262691932851929 1104 90-91 0.5682934807691247 1104 92-93 0.7838518549284395 1104 94-95 1.5245243510504238 1104 96-97 1.7359963719286817 1104 98-99 1.3851936131585454 1104 100-101 1.3782000620392196 1105 1 0.4920315119528915 1105 2 0.4234117053529971 1105 3 0.5812817658810303 1105 4 0.3159411866545767 1105 5 0.4769389787031528 1105 6 0.6191868684084838 1105 7 0.5474918703244356 1105 8 0.558645252823375 1105 9 0.7509241137562341 1105 10-11 0.7840153653142892 1105 12-13 0.7971444456300674 1105 14-15 1.0558180510682433 1105 16-17 1.0539234413330405 1105 18-19 0.9417136054198707 1105 20-21 1.0425615643983122 1105 22-23 1.1231049463776728 1105 24-25 1.1602429384155712 1105 26-27 1.2433231103007572 1105 28-29 1.1902204804084633 1105 30-31 1.19159910741854 1105 32-33 1.185830028426743 1105 34-35 1.3878380699898614 1105 36-37 1.2682938408702285 1105 38-39 1.2693863063078012 1105 40-41 1.2687922126588091 1105 42-43 1.2485815561422626 1105 44-45 1.2150704374037815 1105 46-47 1.3954136027500255 1105 48-49 1.1166579322651629 1105 50-51 1.1764693069170349 1105 52-53 1.4766422749068724 1105 54-55 1.4858255878304902 1105 56-57 1.4360824081297707 1105 58-59 1.5159996146343282 1105 60-61 1.4531724970958244 1105 62-63 1.5638825928252515 1105 64-65 1.489912917931715 1105 66-67 2.121551464454061 1105 68-69 -4.767772159122821 1105 70-71 -5.237416062755976 1105 72-73 -1.498575684042688 1105 74-75 1.3525288427633129 1105 76-77 1.4967413849229878 1105 78-79 1.45191957501401 1105 80-81 1.3697745074328225 1105 82-83 1.4295904166095887 1105 84-85 1.3172427141912308 1105 86-87 1.5538068300822907 1105 88-89 1.68220023914591 1105 90-91 1.6025333431063977 1105 92-93 1.7839723289977414 1105 94-95 2.4436380033714684 1105 96-97 2.413229554477592 1105 98-99 2.107108959022714 1105 100-101 2.0752368411611357 1106 1 0.12469192670337037 1106 2 0.1724796072314163 1106 3 0.08022355478877685 1106 4 0.12331310613191704 1106 5 0.308092675330343 1106 6 0.34206404575366633 1106 7 0.35795877278126653 1106 8 0.2569341937588874 1106 9 0.3487915718687091 1106 10-11 0.40149576091076966 1106 12-13 0.3451403872577785 1106 14-15 0.4938643430624339 1106 16-17 0.39436919853238095 1106 18-19 0.36077065020420207 1106 20-21 0.46567318860062556 1106 22-23 0.4669609689831802 1106 24-25 0.4343298046504813 1106 26-27 0.737325158451263 1106 28-29 0.7142554317174685 1106 30-31 0.6441683036142933 1106 32-33 0.6556980365055551 1106 34-35 0.7976407458605621 1106 36-37 0.7735543163066438 1106 38-39 0.6808011895347761 1106 40-41 0.7852357320640877 1106 42-43 0.9149605967354759 1106 44-45 0.7235128948050971 1106 46-47 0.6583904377885688 1106 48-49 0.6808596276786361 1106 50-51 0.7029188949163938 1106 52-53 0.7988559368292911 1106 54-55 0.7544549863759329 1106 56-57 0.6884987042013009 1106 58-59 0.8914787016902075 1106 60-61 0.910455995261021 1106 62-63 0.8904644572111451 1106 64-65 0.7442609707664083 1106 66-67 1.5686378281335251 1106 68-69 -5.055189545644854 1106 70-71 -6.40623747541089 1106 72-73 -2.556625816319073 1106 74-75 0.38025449490012164 1106 76-77 0.7263433230802185 1106 78-79 0.5977488389125156 1106 80-81 0.5835329110132932 1106 82-83 0.7386684170078368 1106 84-85 0.6689125257803497 1106 86-87 0.6295113559259669 1106 88-89 0.7352032449964163 1106 90-91 0.5388574344766646 1106 92-93 0.6536431077690388 1106 94-95 1.5086096516585101 1106 96-97 1.6513519409522104 1106 98-99 1.4580007578867509 1106 100-101 1.1813265330851692 1107 1 -0.0028430089050281993 1107 2 0.04175175236500195 1107 3 -0.01765217347153225 1107 4 -0.03429679943689479 1107 5 0.004633147068560106 1107 6 0.08771954760518241 1107 7 0.041341783030354406 1107 8 0.056654070012989166 1107 9 0.11679554274567039 1107 10-11 0.16434152330194252 1107 12-13 0.10542031442560784 1107 14-15 0.08722246161374869 1107 16-17 -0.01867645918097338 1107 18-19 0.11029480842005768 1107 20-21 0.20867414937593054 1107 22-23 0.08209428577119837 1107 24-25 0.18793344920785415 1107 26-27 0.28721642620779164 1107 28-29 0.1907496093208394 1107 30-31 0.1204646745374518 1107 32-33 0.20430564948265584 1107 34-35 0.3753018600159095 1107 36-37 0.19146749268877272 1107 38-39 0.18947052802501219 1107 40-41 0.47222496695767546 1107 42-43 0.45983322643399305 1107 44-45 0.3576879918040916 1107 46-47 0.182713533499971 1107 48-49 0.4277820751738659 1107 50-51 0.3830500124872813 1107 52-53 0.4374567432908094 1107 54-55 0.3803165511864819 1107 56-57 0.41493992858779905 1107 58-59 0.3975908052475283 1107 60-61 0.25655428920912016 1107 62-63 0.3536964955045505 1107 64-65 0.4553034979740396 1107 66-67 1.0440609009927293 1107 68-69 -5.47965073744853 1107 70-71 -5.867950702411871 1107 72-73 -1.9847264053161062 1107 74-75 0.43614901110921167 1107 76-77 0.37381815494454074 1107 78-79 0.4173520952281997 1107 80-81 0.3963687216398135 1107 82-83 0.4879655275559678 1107 84-85 0.5312326875640991 1107 86-87 0.7269575611092378 1107 88-89 0.6848195491813165 1107 90-91 0.7059161105725273 1107 92-93 0.7031999470885815 1107 94-95 1.455797970989753 1107 96-97 1.5545647637395383 1107 98-99 1.2704542241723829 1107 100-101 1.1038602769390309 1108 1 0.13412811459802043 1108 2 0.05920108168249527 1108 3 0.05082734654508769 1108 4 0.045532394226178496 1108 5 0.15582651454185736 1108 6 0.24915650185177896 1108 7 0.14381589844047227 1108 8 0.09195328069278474 1108 9 0.2788725214388208 1108 10-11 0.26209614497948763 1108 12-13 0.3134231165922401 1108 14-15 0.3002911836020701 1108 16-17 0.2042625602404584 1108 18-19 0.3565862816846348 1108 20-21 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2314 3 -0.207462802114879 2314 4 -0.21050255482463598 2314 5 -1.4626826981884662 2314 6 -2.985373352279993 2314 7 -0.8646683631452348 2314 8 -0.3056728708985048 2314 9 -0.01732635488725265 2314 10-11 0.04410078624135849 2314 12-13 -0.21421616488458284 2314 14-15 -2.811582202425285 2314 16-17 -3.131007540262054 2314 18-19 -0.6265509757832675 2314 20-21 -0.19048712126971168 2314 22-23 -0.1623418093312594 2314 24-25 -2.309870121826414 2314 26-27 -1.3550558092629714 2314 28-29 -0.22719388564470933 2314 30-31 -0.07782536642262983 2314 32-33 0.0020538802501874898 2314 34-35 -0.08779832656773578 2314 36-37 0.13113866662005336 2314 38-39 -1.3543488888183077 2314 40-41 -4.105032184495247 2314 42-43 -5.215935741062914 2314 44-45 -2.203312369770721 2314 46-47 -3.3219919762377046 2314 48-49 -0.9786977042379021 2314 50-51 -0.9099789079773046 2314 52-53 -2.3007222445205286 2314 54-55 -1.4729175422819836 2314 56-57 -4.0553536646377815 2314 58-59 -5.271454319840789 2314 60-61 -2.618912859925306 2314 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-3.077038127196708 2315 20-21 -2.138652351978081 2315 22-23 -1.6097523944927943 2315 24-25 -1.0909099295921862 2315 26-27 -0.482958423321044 2315 28-29 -0.5299218653434679 2315 30-31 -2.936051343965744 2315 32-33 -2.5529094704101496 2315 34-35 -1.6988403684254152 2315 36-37 -4.946026611839891 2315 38-39 -2.5458233553649166 2315 40-41 -1.9146180588949377 2315 42-43 -1.6625460608620912 2315 44-45 -1.551970976652406 2315 46-47 -1.5201248040864286 2315 48-49 -4.76439115954242 2315 50-51 -4.141018971555127 2315 52-53 -2.7916562654766537 2315 54-55 -4.354032729331749 2315 56-57 -6.424435026512917 2315 58-59 -7.4127133073708045 2315 60-61 -6.320549886502473 2315 62-63 -7.076183939651514 2315 64-65 -6.361909794713075 2315 66-67 -4.40002910372365 2315 68-69 -2.159940732169936 2315 70-71 0.44276039294327774 2315 72-73 -1.3633423985195279 2315 74-75 -2.7443846891228603 2315 76-77 -5.121401671955052 2315 78-79 -4.061108195063248 2315 80-81 -3.9374587901433244 2315 82-83 -7.05767650879449 2315 84-85 -4.824302418210809 2315 86-87 -5.308468833735855 2315 88-89 -3.7781568907973195 2315 90-91 -3.6143757894402775 2315 92-93 -4.515552180747097 2315 94-95 -2.6293416150310307 2315 96-97 -1.5154261158491664 2315 98-99 -2.659311424985386 2315 100-101 -2.1339677220989515 2316 1 -0.4140433228329137 2316 2 -0.3468483375824434 2316 3 -0.9165546370141513 2316 4 -0.44036203474958313 2316 5 -0.3455123917723455 2316 6 -2.564817188336086 2316 7 -0.4922681212006452 2316 8 -0.4460584833075103 2316 9 -0.15303354903544175 2316 10-11 -0.08521039470215896 2316 12-13 -0.6124029251196177 2316 14-15 -1.0375397307462038 2316 16-17 -1.6571470780985251 2316 18-19 -0.2615762679860225 2316 20-21 -4.157579814084734 2316 22-23 -4.196323495078396 2316 24-25 -2.718197349233165 2316 26-27 -2.163467263187698 2316 28-29 -1.3823196897754073 2316 30-31 -0.5851565562891636 2316 32-33 -2.083399003157915 2316 34-35 -2.5200492135803216 2316 36-37 -4.772371050840867 2316 38-39 -2.275337978387185 2316 40-41 -1.794733073447624 2316 42-43 -3.1172807201837323 2316 44-45 -1.032203255760507 2316 46-47 -0.9325801158390234 2316 48-49 -0.6752263196518129 2316 50-51 -0.4777782791329983 2316 52-53 -2.924225080139287 2316 54-55 -1.2459308236642386 2316 56-57 -0.3669011841767471 2316 58-59 -0.3249099436416465 2316 60-61 -1.6222984529364233 2316 62-63 -1.1791829288963989 2316 64-65 0.018757867409352258 2316 66-67 1.1458639677645763 2316 68-69 3.7315947924299273 2316 70-71 3.720151931336506 2316 72-73 2.1066411430346825 2316 74-75 1.1546009138529314 2316 76-77 -0.19161635015477074 2316 78-79 0.09465497992510308 2316 80-81 -0.3892526060781307 2316 82-83 -0.3731373702212615 2316 84-85 -0.0041076956895302885 2316 86-87 -0.19837709748207644 2316 88-89 0.3110160560876487 2316 90-91 0.4316728116167745 2316 92-93 0.6995435915426462 2316 94-95 1.1010400822193773 2316 96-97 1.0399591235341639 2316 98-99 0.12900322816995669 2316 100-101 -0.1615961094506453 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 39.0 4 102.0 5 248.0 6 522.0 7 1320.0 8 2972.0 9 4782.0 10 7243.0 11 10727.0 12 14396.0 13 18299.0 14 22107.0 15 27446.0 16 32305.0 17 36391.0 18 34337.0 19 30440.0 20 29724.0 21 29915.0 22 31506.0 23 36034.0 24 43476.0 25 54922.0 26 68888.0 27 84974.0 28 104339.0 29 124797.0 30 147895.0 31 171148.0 32 198980.0 33 229522.0 34 267384.0 35 308619.0 36 331428.0 37 283084.0 38 119859.0 39 7289.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.884565632310135 19.97146145530807 15.126525795601072 23.017447116780726 2 16.22012631873419 21.08569111048107 38.66218566447267 24.031996906312063 3 19.681113788090833 24.492136500601376 30.097855434183145 25.728894277124642 4 13.405670055565325 17.39872293328425 36.22741919617257 32.96818781497785 5 13.704989574846365 37.66001488279371 33.885913891624334 14.749081650735588 6 32.14274574485332 36.29963937895441 16.886257839420292 14.671357036771967 7 28.45787116015365 32.9206808508888 20.20735795811915 18.414090030838395 8 26.454455183212012 35.07494686315218 19.2156757899918 19.25492216364401 9 26.058323881641165 16.876146328304657 20.24303959564924 36.82249019440494 10-11 25.182341394092887 26.853328589492186 26.743095980177323 21.2212340362376 12-13 26.051297238323517 24.176605939330248 27.582488508010545 22.18960831433569 14-15 22.815630819474563 25.623957357529665 26.24041677249477 25.319995050501 16-17 21.983424277183623 28.88430841970557 26.825513773198907 22.306753529911898 18-19 21.780070202066167 27.07470310826066 29.6034136830103 21.54181300666287 20-21 22.67378220109811 26.141688345007562 29.655627579614023 21.528901874280304 22-23 22.26986629154674 26.165694419165668 29.563162154019224 22.001277135268367 24-25 22.225512747873413 26.323451130724013 29.666299567398706 21.784736554003867 26-27 22.050845685708946 26.505721596391375 29.66506767028112 21.778365047618557 28-29 21.72165907654165 26.81053964623298 29.77231681881199 21.695484458413382 30-31 21.986431871510856 26.384273544000443 29.61765148971593 22.011643094772776 32-33 21.91267317545218 26.48981321877743 29.50852630563206 22.08898730013833 34-35 22.454464991502142 26.47594993291526 29.350635505343124 21.718949570239467 36-37 22.282965238205037 26.30656957848853 29.486433869143692 21.924031314162743 38-39 22.037601447304876 26.635247775472166 29.513267390261916 21.81388338696105 40-41 22.210336772335065 26.505125214186904 29.435860953277142 21.84867706020089 42-43 22.052262773522262 26.595497922719968 29.098853687292152 22.253385616465618 44-45 22.213976232335167 26.665489811799294 28.94949748640269 22.171036469462845 46-47 22.278014249126223 26.935717325937652 28.5992991237417 22.186969301194427 48-49 22.452133493524556 26.960248020756005 28.443692401971088 22.14392608374835 50-51 22.192994645709277 27.189760798219925 28.388994968236243 22.228249587834558 52-53 22.64069974637847 27.159217616945565 28.073418004195638 22.12666463248033 54-55 22.219787007925937 26.965145052167607 28.285494234458657 22.5295737054478 56-57 22.343397546864026 27.328002655338047 28.07755959894932 22.25104019884861 58-59 22.544465365737434 26.972532732491324 28.08586167206745 22.397140229703798 60-61 22.483751139147085 27.015829709572987 28.43667627689454 22.06374287438539 62-63 22.30224162294221 27.05656629536225 28.365754987073327 22.275437094622212 64-65 22.175905184632676 27.01700491510568 28.273255058644313 22.533834841617324 66-67 21.795658601479715 27.11897455843403 28.59275423925708 22.49261260082918 68-69 21.73272219016389 26.700777117030576 29.18860550858146 22.377895184224077 70-71 21.330504038592863 26.756704458421837 29.450004677639726 22.462786825345578 72-73 22.172443227857965 26.896313022323554 28.364992899776603 22.56625085004187 74-75 21.855533575061557 27.25470266433108 28.18344432853481 22.70631943207255 76-77 22.06780267619409 27.24491121690281 27.845243127224634 22.84204297967846 78-79 22.090485400151238 27.23777964104206 27.88982120661369 22.781913752193013 80-81 22.02245477168183 27.375352194747414 27.820180159181746 22.782012874389014 82-83 22.08148369801612 27.411449726766403 27.74422127139741 22.762845303820058 84-85 21.996152827092814 27.268328999094077 27.669241845751813 23.066276328061296 86-87 22.01440464101938 27.22026418505808 27.756397897145206 23.008933276777334 88-89 21.96511404312466 27.17113478247606 27.58756022813081 23.276190946268468 90-91 21.832843906316572 27.263684218283878 27.743999106040157 23.15947276935939 92-93 21.984180521392904 27.189317533049795 27.739993199461445 23.08650874609586 94-95 21.48729498285111 27.06803491337613 28.04102472847595 23.403645375296808 96-97 21.2598085711822 26.929429818880894 28.24859911908802 23.562162490848884 98-99 21.59006355543999 26.967242183438493 27.607235985416136 23.83545827570538 100-101 21.422014386121752 26.76146694016569 27.909035065828984 23.907483607883574 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2751.0 1 1897.0 2 1121.0 3 1244.0 4 1183.0 5 1162.0 6 1679.5 7 2936.0 8 4347.0 9 5242.0 10 4983.5 11 4234.5 12 3958.0 13 3982.5 14 4252.0 15 4408.0 16 4553.0 17 4820.0 18 5195.5 19 5704.5 20 6227.5 21 7064.0 22 8269.5 23 9788.5 24 11623.0 25 14072.0 26 17639.0 27 21802.0 28 25575.5 29 29365.5 30 33794.5 31 38667.0 32 44025.0 33 49122.0 34 54387.0 35 59884.5 36 65638.0 37 71625.0 38 75351.0 39 77136.0 40 79722.5 41 84462.0 42 90042.5 43 94921.5 44 99360.0 45 103089.5 46 105677.5 47 110048.5 48 118004.5 49 119390.0 50 113721.5 51 109224.5 52 105384.0 53 103149.0 54 99554.0 55 91843.5 56 82835.0 57 74087.5 58 66207.5 59 58011.5 60 48947.5 61 39832.0 62 31636.0 63 24749.0 64 18735.0 65 13957.0 66 10444.0 67 8129.0 68 6051.0 69 4146.5 70 2988.0 71 2336.5 72 1928.0 73 1603.0 74 1247.0 75 944.5 76 670.5 77 397.5 78 242.0 79 144.5 80 91.0 81 65.5 82 37.5 83 23.5 84 13.5 85 11.5 86 11.5 87 5.5 88 2.5 89 3.0 90 1.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024336179295258522 2 0.01919473296527433 3 0.0 4 0.0 5 1.371052354662452E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.5707231649920977E-4 18-19 0.001079703729296681 20-21 1.713815443328065E-5 22-23 0.0 24-25 0.0 26-27 4.113157063987356E-4 28-29 0.017789404301745314 30-31 0.00395891367408783 32-33 0.00790068919374238 34-35 0.0089461166141725 36-37 0.10293175552628359 38-39 0.10572527469890833 40-41 0.12133813338762701 42-43 0.10553675500014224 44-45 0.10005254558149243 46-47 0.10188632810585346 48-49 0.1039771829467137 50-51 0.10221195304008579 52-53 0.07871554331205803 54-55 0.06555344070729849 56-57 0.0369327228037198 58-59 0.015407200835519306 60-61 0.00899753107747234 62-63 0.0016966772888947846 64-65 0.004593025388119214 66-67 0.4175539946124498 68-69 0.8033338509055972 70-71 0.8929492604372218 72-73 0.2759757008391183 74-75 0.14663404933114926 76-77 0.01419039187075638 78-79 0.008397695672307519 80-81 0.001610986516728381 82-83 0.0 84-85 0.0 86-87 1.5424338989952587E-4 88-89 6.169735595981035E-4 90-91 0.003770393975321743 92-93 0.0017480917521946263 94-95 0.22248752085284942 96-97 0.2269091646966358 98-99 0.01396759586312373 100-101 0.007232301170844435 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2917467.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.82869178245423 #Duplication Level Percentage of deduplicated Percentage of total 1 75.56458209384252 41.43107181293251 2 13.889682562986986 15.231062484042848 3 4.651174001215926 7.650533572176972 4 2.0608641216576995 4.519779349275531 5 1.08707959674997 2.9801576076599368 6 0.6437646954466756 2.117806564024277 7 0.40015230740981084 1.5357879270307275 8 0.2779200055908675 1.2190392261375704 9 0.21350628898129712 1.053564346095403 >10 1.0725767560710138 10.753174609196007 >50 0.08371659945753443 3.1545673611712406 >100 0.05056310769528961 5.034271758365354 >500 0.003344953334460127 1.2547288306220352 >1k 8.835725788216497E-4 0.8892449389628406 >5k 6.311232705868926E-5 0.28617229532347627 >10k+ 1.262246541173785E-4 0.8890373169831622 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13463 0.4614619462705148 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12229 0.41916498112917816 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8270 0.28346507432646195 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3372 0.1155797134980447 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3363 0.11527122671824565 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3200 0.10968418837299616 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.42763088665613E-5 0.0 12-13 0.0 0.0 0.0 5.141446329984195E-5 0.0 14-15 0.0 0.0 0.0 6.85526177331226E-5 0.0 16-17 0.0 3.42763088665613E-5 0.0 6.85526177331226E-5 0.0 18-19 0.0 3.42763088665613E-5 0.0 6.85526177331226E-5 0.0 20-21 3.42763088665613E-5 3.42763088665613E-5 0.0 1.713815443328065E-4 0.0 22-23 3.42763088665613E-5 3.42763088665613E-5 0.0 4.970064785651388E-4 0.0 24-25 3.42763088665613E-5 3.42763088665613E-5 0.0 0.0010968418837299615 0.0 26-27 3.42763088665613E-5 3.42763088665613E-5 0.0 0.0016624009800282231 0.0 28-29 3.42763088665613E-5 3.42763088665613E-5 0.0 0.003221973033456762 0.0 30-31 3.42763088665613E-5 3.42763088665613E-5 0.0 0.006786709155579137 0.0 32-33 3.42763088665613E-5 3.42763088665613E-5 0.0 0.014378911569522465 0.0 34-35 3.42763088665613E-5 3.42763088665613E-5 0.0 0.027763810181914657 0.0 36-37 3.42763088665613E-5 3.42763088665613E-5 0.0 0.04988916755527997 0.0 38-39 3.42763088665613E-5 3.42763088665613E-5 0.0 0.08231455574304697 0.0 40-41 3.42763088665613E-5 3.42763088665613E-5 0.0 0.11455142423204787 0.0 42-43 3.42763088665613E-5 3.42763088665613E-5 0.0 0.15014737098997177 0.0 44-45 3.42763088665613E-5 3.42763088665613E-5 0.0 0.19417528972906978 0.0 46-47 3.42763088665613E-5 3.42763088665613E-5 0.0 0.24946297593083314 0.0 48-49 3.42763088665613E-5 3.42763088665613E-5 0.0 0.30812687855595283 0.0 50-51 3.42763088665613E-5 3.42763088665613E-5 0.0 0.3701669976044287 0.0 52-53 3.42763088665613E-5 3.42763088665613E-5 0.0 0.43191576802753895 0.0 54-55 3.42763088665613E-5 3.42763088665613E-5 0.0 0.5001598989808624 0.0 56-57 3.42763088665613E-5 3.42763088665613E-5 0.0 0.5820288627086442 0.0 58-59 3.42763088665613E-5 3.42763088665613E-5 0.0 0.6644291092238576 0.0 60-61 3.42763088665613E-5 5.141446329984195E-5 0.0 0.7462295203339061 0.0 62-63 6.85526177331226E-5 6.85526177331226E-5 0.0 0.8292638785631509 0.0 64-65 6.85526177331226E-5 6.85526177331226E-5 1.713815443328065E-5 0.921055833707802 0.0 66-67 6.85526177331226E-5 6.85526177331226E-5 6.85526177331226E-5 1.0178521299469712 0.0 68-69 6.85526177331226E-5 6.85526177331226E-5 6.85526177331226E-5 1.1365681257063063 0.0 70-71 6.85526177331226E-5 6.85526177331226E-5 6.85526177331226E-5 1.2875381281090754 0.0 72-73 6.85526177331226E-5 6.85526177331226E-5 6.85526177331226E-5 1.4495622401213106 0.0 74-75 6.85526177331226E-5 6.85526177331226E-5 6.85526177331226E-5 1.6246456258117057 0.0 76-77 6.85526177331226E-5 6.85526177331226E-5 6.85526177331226E-5 1.8277327558460814 0.0 78-79 6.85526177331226E-5 6.85526177331226E-5 6.85526177331226E-5 2.0628168202073924 0.0 80-81 6.85526177331226E-5 6.85526177331226E-5 6.85526177331226E-5 2.3222713401728283 0.0 82-83 6.85526177331226E-5 6.85526177331226E-5 6.85526177331226E-5 2.5884954311394095 0.0 84-85 6.85526177331226E-5 6.85526177331226E-5 6.85526177331226E-5 2.8505378124242706 0.0 86-87 1.028289265996839E-4 6.85526177331226E-5 1.028289265996839E-4 3.122263250963935 0.0 88-89 1.028289265996839E-4 6.85526177331226E-5 1.028289265996839E-4 3.425745689668469 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11000 0.0 33.47892 1 GTATCAA 15770 0.0 30.144798 1 ATCAACG 19685 0.0 24.0645 3 TATCAAC 19765 0.0 24.041887 2 TCAACGC 19900 0.0 23.828314 4 CAACGCA 20150 0.0 23.48566 5 AACGCAG 20620 0.0 22.950342 6 GTACATG 12775 0.0 22.779835 1 TACATGG 12815 0.0 21.51636 2 GAGTACT 12295 0.0 21.192211 12-13 ACATGGG 12950 0.0 20.631067 3 ACGCAGA 23380 0.0 20.180279 7 CGCAGAG 23405 0.0 20.098005 8 GTACTTT 13000 0.0 19.915394 14-15 AGTACTT 12565 0.0 19.624578 12-13 AGAGTAC 19260 0.0 19.357006 10-11 GCAGAGT 24370 0.0 18.660704 9 CATGGGA 7345 0.0 17.735899 4 GTGTAGC 1635 0.0 17.682968 1 ACTTTTT 15360 0.0 17.47218 16-17 >>END_MODULE