FastQCFastQC Report
Fri 27 May 2016
SRR939388_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939388_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2894792
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92110.3181921188119906No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68910.23804819137264438No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48520.16761135169642585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141750.033.975091
GGTATCA94850.032.80411
TATCAAC176200.026.991872
ATCAACG182200.026.1865123
TCAACGC185050.025.8506954
CAACGCA185650.025.5671335
AACGCAG190700.024.8898746
GTACATG115100.023.9658181
TACATGG115200.023.1720092
ACGCAGA208550.022.3639077
CGCAGAG212500.022.0178958
GAGTACT105450.021.55309712-13
ACATGGG122500.021.223163
AGAGTAC164350.020.52396210-11
GCAGAGT212750.020.4863439
AGTACTT110400.020.4560112-13
AACGCGG4050.020.3717546
GTACTTT116200.019.98792614-15
CATGGGA72000.017.9513514
ACTTTTT146750.017.6941616-17
Produced by FastQC (version 0.11.5)