FastQCFastQC Report
Fri 27 May 2016
SRR939388_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939388_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2894792
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133670.4617602922766126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115680.3996142037148092No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80600.2784310582591081No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32920.11372146945272751No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32080.11081970656268224No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31700.10950700430289982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111350.032.6100431
GTATCAA160850.029.125691
TATCAAC199500.023.4789852
ATCAACG199000.023.4623763
TCAACGC200700.023.2680644
CAACGCA203550.022.966275
AACGCAG208450.022.4718976
GTACATG131100.022.1217081
TACATGG133350.021.5311582
GAGTACT120450.021.01534812-13
ACATGGG133250.020.8670183
AGTACTT125000.020.04181912-13
ACGCAGA234800.019.8288757
GTACTTT128300.019.65563814-15
CGCAGAG236550.019.6420968
AGAGTAC192900.019.03411110-11
GCAGAGT245450.018.4662869
TAGCGCG2854.3221007E-818.3172554
CATGGGA74200.018.228614
ACTTTTT150000.017.36515216-17
Produced by FastQC (version 0.11.5)