##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939388_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2894792 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.899894362012883 31.0 31.0 31.0 28.0 34.0 2 31.151743544959363 31.0 31.0 34.0 28.0 34.0 3 31.1545199102388 31.0 31.0 34.0 28.0 34.0 4 35.058265671592295 35.0 35.0 37.0 32.0 37.0 5 34.61939027052721 35.0 35.0 37.0 30.0 37.0 6 34.67722205947785 35.0 35.0 37.0 31.0 37.0 7 34.52035379398589 35.0 35.0 37.0 30.0 37.0 8 34.41753362590473 35.0 35.0 37.0 30.0 37.0 9 35.93180857208394 37.0 35.0 39.0 30.0 39.0 10-11 35.72671404370331 37.0 35.0 39.0 30.0 39.0 12-13 35.70979417519463 37.0 35.0 39.0 30.0 39.0 14-15 36.54885981445299 38.0 35.0 40.0 30.5 41.0 16-17 36.46081635571744 38.0 35.0 40.0 30.0 41.0 18-19 36.45135799048774 38.0 35.0 40.0 30.0 41.0 20-21 36.32894332304359 38.0 34.5 40.0 29.5 41.0 22-23 36.16975191999978 38.0 34.0 40.0 29.0 41.0 24-25 35.9963807416906 38.0 34.0 40.0 29.0 41.0 26-27 35.52435425412257 38.0 34.0 40.0 27.0 41.0 28-29 35.31668423845306 38.0 34.0 40.0 27.0 41.0 30-31 35.021591706761654 37.5 33.0 40.0 27.0 41.0 32-33 34.79091727488538 37.0 33.0 40.0 25.5 41.0 34-35 35.04266506885469 38.0 33.0 40.0 26.5 41.0 36-37 34.886987562491534 38.0 33.0 40.0 25.5 41.0 38-39 34.704070102446046 38.0 33.0 40.0 25.0 41.0 40-41 34.54852334813693 37.5 33.0 40.0 25.0 41.0 42-43 34.38448928282239 37.0 33.0 40.0 24.5 41.0 44-45 34.001556243073765 37.0 33.0 40.0 24.0 41.0 46-47 33.604823766267145 37.0 32.0 40.0 23.0 41.0 48-49 33.208242250220394 37.0 31.0 40.0 21.0 41.0 50-51 32.97223392906986 37.0 31.0 39.0 20.0 40.5 52-53 32.8640035277146 36.0 31.0 39.0 20.0 40.0 54-55 32.51685665153144 36.0 31.0 39.0 18.5 40.0 56-57 32.0119635538581 35.5 30.0 39.0 16.5 40.0 58-59 31.54036144911275 35.0 29.0 39.0 13.5 40.0 60-61 31.15837459133506 35.0 29.0 38.0 12.5 40.0 62-63 31.010880574493783 35.0 29.0 38.0 11.0 40.0 64-65 30.561372976020383 34.0 28.0 38.0 8.0 40.0 66-67 29.70311891147965 34.0 27.0 37.5 7.0 39.5 68-69 26.293636641250906 31.0 19.0 36.0 2.0 39.0 70-71 24.982701520523754 30.0 13.5 35.0 2.0 38.0 72-73 25.97987592890957 31.0 19.5 35.0 2.0 38.0 74-75 26.711484452078075 31.0 22.5 35.0 2.0 37.0 76-77 26.381420323118206 30.5 23.0 34.0 2.0 36.0 78-79 27.482051214733218 32.0 26.0 35.0 2.0 37.0 80-81 27.496495948586286 32.0 26.0 35.0 2.0 36.5 82-83 27.171622347995985 32.0 25.5 35.0 2.0 36.0 84-85 26.81718617434344 32.0 25.0 34.5 2.0 36.0 86-87 26.377425735596894 32.0 24.5 34.0 2.0 35.0 88-89 25.89675113099663 31.5 23.5 34.0 2.0 35.0 90-91 25.31056946405821 31.0 20.5 34.0 2.0 35.0 92-93 24.74192567203447 31.0 19.0 34.0 2.0 35.0 94-95 23.906956527446532 31.0 13.0 34.0 2.0 35.0 96-97 23.240444218444708 30.0 4.5 34.0 2.0 35.0 98-99 22.38508414421485 30.0 2.0 34.0 2.0 35.0 100-101 20.9627527642746 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.312817010566107 1101 2 0.23265906370459177 1101 3 0.3890884031377979 1101 4 0.3584626957200783 1101 5 0.5412992091942712 1101 6 0.4935338884619327 1101 7 0.6362463928157496 1101 8 0.7356023800223142 1101 9 0.8265918632261346 1101 10-11 0.870377471429201 1101 12-13 0.7226549656872194 1101 14-15 0.748865444446551 1101 16-17 0.808599171979715 1101 18-19 0.6169751647702029 1101 20-21 0.8219918959996093 1101 22-23 0.7044827949416046 1101 24-25 0.8175895105908069 1101 26-27 0.7163857093094066 1101 28-29 0.7288665438067952 1101 30-31 0.5717121395106446 1101 32-33 0.8130835496726618 1101 34-35 0.8315131227102057 1101 36-37 0.9142439642099092 1101 38-39 0.7923718008710452 1101 40-41 0.8871306645971941 1101 42-43 0.7252903630594432 1101 44-45 0.9663193299892896 1101 46-47 0.9688571189963966 1101 48-49 1.0147125535103356 1101 50-51 0.6453905757919429 1101 52-53 0.5110931818623285 1101 54-55 0.5052563234632075 1101 56-57 0.5774499229099099 1101 58-59 0.5410690285378976 1101 60-61 0.45720573848273105 1101 62-63 0.19558099133296025 1101 64-65 0.0705812159278203 1101 66-67 -0.03337648114669278 1101 68-69 -7.08448441157741 1101 70-71 -8.036776280752466 1101 72-73 -5.129918975921573 1101 74-75 -2.855027423966458 1101 76-77 -0.37160856229899153 1101 78-79 -0.05709217287467183 1101 80-81 -0.08637549353466412 1101 82-83 -0.4067734260432907 1101 84-85 -0.33010972629803703 1101 86-87 -0.3468937262993741 1101 88-89 -0.40760761852966354 1101 90-91 -0.6757815087173213 1101 92-93 -0.6053600575007785 1101 94-95 -0.6869995668472306 1101 96-97 -0.8463569024791937 1101 98-99 -0.8859590089970375 1101 100-101 -0.8333155077270362 1102 1 0.25773575654381986 1102 2 0.29381807562129936 1102 3 0.31960508724929326 1102 4 0.33272118817991725 1102 5 0.45507806606175194 1102 6 0.3897524020806671 1102 7 0.375791210678436 1102 8 0.5334219298945726 1102 9 0.44620026007194724 1102 10-11 0.5205661927590555 1102 12-13 0.4925711793510743 1102 14-15 0.6384606016392951 1102 16-17 0.5456430869062459 1102 18-19 0.6301366486345117 1102 20-21 0.49111746500285847 1102 22-23 0.5974558541916153 1102 24-25 0.49854681731387984 1102 26-27 0.5792808142188264 1102 28-29 0.4204929748199149 1102 30-31 0.3144361111610081 1102 32-33 0.5926732534379795 1102 34-35 0.4207801534161746 1102 36-37 0.5334377266348724 1102 38-39 0.6304829198263704 1102 40-41 0.5222165637527851 1102 42-43 0.4729010354066858 1102 44-45 0.45042095200026466 1102 46-47 0.40163298517563106 1102 48-49 0.4196066720962861 1102 50-51 0.29887775102908876 1102 52-53 0.07546240038516316 1102 54-55 -0.13680320995668183 1102 56-57 0.052477369099516835 1102 58-59 0.14284748023516514 1102 60-61 0.26946285377309565 1102 62-63 0.07245298516946264 1102 64-65 0.05415335413179179 1102 66-67 -0.2225835036878685 1102 68-69 -7.084134287212663 1102 70-71 -7.601847385056171 1102 72-73 -1.6675974017329622 1102 74-75 -0.02863641628971081 1102 76-77 0.39856615200996615 1102 78-79 0.287729270861405 1102 80-81 0.027495600946732424 1102 82-83 -0.08798094583882232 1102 84-85 -0.2724510883452531 1102 86-87 -0.20336780410228883 1102 88-89 -0.34173512641564585 1102 90-91 -0.35138130899705544 1102 92-93 -0.2566022213962249 1102 94-95 -0.2986067727381574 1102 96-97 -0.6222657985233617 1102 98-99 -0.8255308893654174 1102 100-101 -0.7122853829121034 1103 1 0.3642403107269061 1103 2 0.21746571927324254 1103 3 0.30183795658446044 1103 4 0.3371022573079827 1103 5 0.47945369842231145 1103 6 0.4899521459115732 1103 7 0.4677999933925179 1103 8 0.4601225123884163 1103 9 0.5059216079312847 1103 10-11 0.559430029035596 1103 12-13 0.46842222735330097 1103 14-15 0.6069722608149135 1103 16-17 0.6666820911626772 1103 18-19 0.6517093187085834 1103 20-21 0.642386321069786 1103 22-23 0.6181600857298477 1103 24-25 0.5857350687521077 1103 26-27 0.6618215494169846 1103 28-29 0.676948592160123 1103 30-31 0.5152310405641458 1103 32-33 0.750549374672353 1103 34-35 0.740612988642944 1103 36-37 0.8808826833207704 1103 38-39 0.8112726518538764 1103 40-41 0.8600412810230651 1103 42-43 0.5163408671280436 1103 44-45 0.7387537241146376 1103 46-47 0.8093800623195619 1103 48-49 0.9198962175564134 1103 50-51 0.7401617501158384 1103 52-53 0.4755868248059798 1103 54-55 0.31098121825484526 1103 56-57 0.5679121106769749 1103 58-59 0.825210917063611 1103 60-61 0.7500779979008563 1103 62-63 0.3812256685386899 1103 64-65 0.29715124075659105 1103 66-67 0.31239412207496997 1103 68-69 -6.802756442554642 1103 70-71 -7.739696496556203 1103 72-73 -0.8850505927079304 1103 74-75 0.49165046222622877 1103 76-77 0.7310224570577297 1103 78-79 0.6032925718102291 1103 80-81 0.3327570386354033 1103 82-83 0.2838891537996382 1103 84-85 0.1401492138820366 1103 86-87 0.16943483569761852 1103 88-89 0.2882219175814136 1103 90-91 0.1468938824471948 1103 92-93 0.14053519587013952 1103 94-95 0.4308106206444151 1103 96-97 0.3021970140264685 1103 98-99 0.4070087379034142 1103 100-101 0.48622449170591153 1104 1 0.30268250409361386 1104 2 0.3651550183971466 1104 3 0.2684876749824596 1104 4 0.25695480737056187 1104 5 0.4481317334661128 1104 6 0.3735480470379855 1104 7 0.35350165171542614 1104 8 0.5084806162682654 1104 9 0.5667132224494367 1104 10-11 0.42273689699554495 1104 12-13 0.3748186995059939 1104 14-15 0.609042426647207 1104 16-17 0.5379878248923333 1104 18-19 0.5771111882329834 1104 20-21 0.5339048207568879 1104 22-23 0.5852003313493697 1104 24-25 0.5047512217558605 1104 26-27 0.7196416761378899 1104 28-29 0.6000184451012984 1104 30-31 0.4543168118407408 1104 32-33 0.5752695326823698 1104 34-35 0.3362334908331803 1104 36-37 0.6253038347600679 1104 38-39 0.54774852012671 1104 40-41 0.5693136136263135 1104 42-43 0.47857666969374435 1104 44-45 0.7679202604100013 1104 46-47 0.6069209337213124 1104 48-49 0.7067301505977142 1104 50-51 0.6110767610670322 1104 52-53 0.6346055200500373 1104 54-55 0.4679970192560958 1104 56-57 0.5226117352076436 1104 58-59 0.5961130212563397 1104 60-61 0.6001749941461618 1104 62-63 0.27675210783781523 1104 64-65 0.29425738906697774 1104 66-67 0.3091179565555713 1104 68-69 -6.5938644682117165 1104 70-71 -4.508821183665088 1104 72-73 0.16734791323700193 1104 74-75 0.9299285833150925 1104 76-77 1.1395853090602337 1104 78-79 0.6735530536625411 1104 80-81 0.4317737783099922 1104 82-83 0.14116093884344139 1104 84-85 0.3294457955466221 1104 86-87 0.08723501561000901 1104 88-89 0.20787832402697148 1104 90-91 0.1396275729978882 1104 92-93 0.24350472809792478 1104 94-95 0.3306930909197625 1104 96-97 0.25528700933310233 1104 98-99 0.5351251333977807 1104 100-101 0.705571527062606 1105 1 0.11456500721179452 1105 2 0.17916382725277558 1105 3 0.20058055773892747 1105 4 0.2323166135757404 1105 5 0.32521818497843213 1105 6 0.165550502603935 1105 7 0.15899880637709884 1105 8 0.41741946098295557 1105 9 0.403011164451307 1105 10-11 0.3626652763220193 1105 12-13 0.3058213402138179 1105 14-15 0.4462199874516912 1105 16-17 0.40697378334260037 1105 18-19 0.548781801344937 1105 20-21 0.4850703863166004 1105 22-23 0.4729555835320909 1105 24-25 0.4850466204929944 1105 26-27 0.5301383923056449 1105 28-29 0.5124302591019259 1105 30-31 0.4760058416973081 1105 32-33 0.45815379952053803 1105 34-35 0.4784397486286309 1105 36-37 0.5440121291161688 1105 38-39 0.5579557328644782 1105 40-41 0.6947299392421584 1105 42-43 0.5471484949977778 1105 44-45 0.6134545408902028 1105 46-47 0.7463101116913862 1105 48-49 0.5671174868933164 1105 50-51 0.3176257651986134 1105 52-53 0.40382646251963905 1105 54-55 0.4625602793122212 1105 56-57 0.7430311302809223 1105 58-59 0.6697505232052059 1105 60-61 0.634308919933499 1105 62-63 0.3177341393314208 1105 64-65 0.5289984827682588 1105 66-67 0.6632032095178388 1105 68-69 -2.9708264848939727 1105 70-71 -3.284963801376346 1105 72-73 0.6164248835363466 1105 74-75 1.0084020885380411 1105 76-77 1.031569104273828 1105 78-79 0.8555664228549347 1105 80-81 0.8164948961771579 1105 82-83 0.6372542016279787 1105 84-85 0.6478464133358308 1105 86-87 0.4918207362991289 1105 88-89 0.6361643487385393 1105 90-91 0.7164858615073939 1105 92-93 0.5349480064733534 1105 94-95 0.8346820701059166 1105 96-97 0.6885992462367483 1105 98-99 0.7622236678536609 1105 100-101 0.6523077193762958 1106 1 0.12615314654733467 1106 2 0.21813627035824013 1106 3 0.16248183092848834 1106 4 0.2355370359554385 1106 5 0.28633105885048593 1106 6 0.2373978666175276 1106 7 0.3487061936043432 1106 8 0.40248889207623506 1106 9 0.5188806212570896 1106 10-11 0.39142424010818644 1106 12-13 0.293397251043487 1106 14-15 0.2634971450343997 1106 16-17 0.35689976205895135 1106 18-19 0.3285155751956452 1106 20-21 0.33337841465397133 1106 22-23 0.4101855741325551 1106 24-25 0.28289029259050125 1106 26-27 0.5906166820211638 1106 28-29 0.4599018581857024 1106 30-31 0.4293610834912016 1106 32-33 0.5741342075333264 1106 34-35 0.3991578221008325 1106 36-37 0.47749947329160847 1106 38-39 0.5891197646356545 1106 40-41 0.45071878502574947 1106 42-43 0.4810497537069267 1106 44-45 0.5721396477648781 1106 46-47 0.6148650720175226 1106 48-49 0.7560417092735818 1106 50-51 0.7498219073644847 1106 52-53 0.4903547552241818 1106 54-55 0.6286345702444791 1106 56-57 0.577454847852767 1106 58-59 0.773125836992147 1106 60-61 1.0414732370077893 1106 62-63 0.5889318615246673 1106 64-65 0.6220134096192211 1106 66-67 0.6542401816104828 1106 68-69 -2.977928144866876 1106 70-71 -2.993125192137004 1106 72-73 1.0341071597854814 1106 74-75 1.392426279032069 1106 76-77 1.4250442536005536 1106 78-79 1.2237426912527525 1106 80-81 0.8646301087147634 1106 82-83 0.6288509196247425 1106 84-85 0.6515830425657612 1106 86-87 0.6093748120486673 1106 88-89 0.6243840994427217 1106 90-91 0.7195146631296723 1106 92-93 0.7222325187317509 1106 94-95 1.192685805463217 1106 96-97 1.0724361209584572 1106 98-99 1.167539629789374 1106 100-101 1.1630604959204653 1107 1 0.033955916145732346 1107 2 0.08910045813655998 1107 3 0.10776902677399036 1107 4 -0.12075997275973549 1107 5 0.15478075626742083 1107 6 0.21227565290117667 1107 7 0.09622762891412151 1107 8 0.11956978564937515 1107 9 0.2682034504624653 1107 10-11 0.1186866119792569 1107 12-13 0.1575869201900275 1107 14-15 0.13753489407390873 1107 16-17 0.10206712049494371 1107 18-19 0.2638401173860885 1107 20-21 0.28853786228783207 1107 22-23 0.2922946920985581 1107 24-25 0.1893740841337106 1107 26-27 0.6238675515450538 1107 28-29 0.4286778506387563 1107 30-31 0.363652622101327 1107 32-33 0.45957480548367613 1107 34-35 0.32724275386249957 1107 36-37 0.4070546490923235 1107 38-39 0.49009430595733505 1107 40-41 0.32318423659048534 1107 42-43 0.08714904347225172 1107 44-45 0.28126614803215233 1107 46-47 0.35241920326529197 1107 48-49 0.3816680577638394 1107 50-51 0.34945064136018544 1107 52-53 0.24396321548977795 1107 54-55 0.10595100948410874 1107 56-57 0.4583934941652963 1107 58-59 0.5714871051325652 1107 60-61 0.5362254827455111 1107 62-63 0.20824030002022198 1107 64-65 0.11350311382267719 1107 66-67 0.5498493287054096 1107 68-69 -2.791457098504875 1107 70-71 -2.873729571287239 1107 72-73 1.0633144238233214 1107 74-75 1.2805154114909882 1107 76-77 1.0479164369103948 1107 78-79 0.9374109526853438 1107 80-81 0.7068979639777488 1107 82-83 0.6904051367261772 1107 84-85 0.6970399527127462 1107 86-87 0.5020869089324869 1107 88-89 0.5992591261442364 1107 90-91 0.4710613067112419 1107 92-93 0.5974313364041137 1107 94-95 0.8136598050891415 1107 96-97 0.7728903277370129 1107 98-99 0.9030567294889913 1107 100-101 1.1250091288541384 1108 1 0.029588268342319424 1108 2 0.05122367124688765 1108 3 0.01992873715862231 1108 4 0.02160344726693353 1108 5 -0.07617526135253883 1108 6 -0.11065855917963319 1108 7 0.1544504859123137 1108 8 0.22141131575013162 1108 9 0.26324171985876177 1108 10-11 0.2604619617623811 1108 12-13 0.08479036401376305 1108 14-15 0.17081218053321834 1108 16-17 0.26874964484051844 1108 18-19 0.2403681506682247 1108 20-21 0.16239980132683485 1108 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-2.3909719867062336 2316 46-47 -2.642949435622075 2316 48-49 -2.5838978995353834 2316 50-51 -1.3387212369365216 2316 52-53 -0.9072830669064871 2316 54-55 -0.12884027000743714 2316 56-57 -3.379575487507527 2316 58-59 -0.7459185060284845 2316 60-61 -0.43463645217149605 2316 62-63 -0.2524143739723037 2316 64-65 -0.19627971164287317 2316 66-67 -0.29134663417202233 2316 68-69 2.5206721888424077 2316 70-71 3.6472219618539903 2316 72-73 2.3836876148785286 2316 74-75 0.9860417432529793 2316 76-77 -1.5287225602831533 2316 78-79 -0.1905402249532493 2316 80-81 -0.03393107972017262 2316 82-83 0.17185844882569867 2316 84-85 0.3383950246948011 2316 86-87 0.6083365295851628 2316 88-89 0.2989504458104655 2316 90-91 0.1653680621283229 2316 92-93 0.03501634633217421 2316 94-95 0.1770707805825822 2316 96-97 0.44455705702609194 2316 98-99 0.49528928601535327 2316 100-101 0.4533159536375848 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 46.0 4 111.0 5 224.0 6 580.0 7 1458.0 8 3117.0 9 5121.0 10 8000.0 11 11583.0 12 15195.0 13 19399.0 14 23480.0 15 28861.0 16 34428.0 17 38225.0 18 35931.0 19 32769.0 20 32623.0 21 33212.0 22 35262.0 23 39149.0 24 46749.0 25 57486.0 26 72117.0 27 89112.0 28 108402.0 29 129903.0 30 151715.0 31 175094.0 32 202295.0 33 233592.0 34 268404.0 35 300159.0 36 306012.0 37 248122.0 38 101170.0 39 5681.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.68804510704077 20.108137765952115 15.182165042596218 23.021652084410892 2 16.225187414895824 21.215639162794332 38.55980502009677 23.99936840221308 3 19.628938602179026 24.666252357262753 30.147469045111247 25.55733999544697 4 13.358512565065942 17.489158269555396 36.344986594358495 32.807342571020165 5 13.663192381352443 37.730482880980745 33.88295255755854 14.723372180108276 6 32.12462242537633 36.296597475742644 16.918797620001712 14.65998247887931 7 28.365526780507892 32.99010775212865 20.26535930733538 18.379006160028077 8 26.438065325591616 35.095854900801164 19.219308330270362 19.24677144333686 9 26.05682895351376 16.865356820110044 20.348266818479534 36.72954740789666 10-11 25.14304923408804 26.920376802841844 26.73184409654588 21.204729866524232 12-13 25.94015390397652 24.222914807005132 27.621725498757772 22.21520579026058 14-15 22.737851286033678 25.63957963128266 26.24976164090546 25.3728074417782 16-17 22.017297696524967 28.936566303777646 26.797784355521504 22.248351644175884 18-19 21.751243657351825 27.15022185558634 29.60373443517549 21.494800051886347 20-21 22.672820706979984 26.162276944250223 29.6616302656633 21.503272083106488 22-23 22.29411006751101 26.23483178555106 29.529132886115626 21.9419252608223 24-25 22.185766544016676 26.351260084423632 29.717928052324826 21.74504531923486 26-27 22.078648890657647 26.516311947954325 29.696395604638564 21.708643556749465 28-29 21.801480780341702 26.807167785211718 29.719985180105763 21.67136625434082 30-31 21.984035440258644 26.387865497530562 29.63050476598105 21.99759429622974 32-33 21.949046126210153 26.490538988349698 29.51797635394806 22.042438531492085 34-35 22.44136718358661 26.515626781266516 29.304887190772135 21.73811884437474 36-37 22.238201729728384 26.297071549238897 29.506912620848986 21.957814100183732 38-39 22.011801888717144 26.683212824742125 29.560394341455993 21.744590945084735 40-41 22.18825071353709 26.469248543582246 29.495776340975222 21.84672440190544 42-43 22.02072565730994 26.62399558560717 29.107056978097795 22.248221778985098 44-45 22.214773546822588 26.68260736983238 28.969283491207605 22.133335592137428 46-47 22.28449395759034 26.941995667657352 28.588085348982457 22.18542502576985 48-49 22.439443289485215 26.941779164996454 28.49116460511198 22.12761294040635 50-51 22.17658484492981 27.181650340289625 28.429364558195637 22.212400256584928 52-53 22.51857881565667 27.221784395868763 28.117369723616353 22.142267064858213 54-55 22.180229071771652 27.03442989027518 28.274005479501298 22.511335558451872 56-57 22.28906451524287 27.338184933133995 28.12553181894909 22.24721873267404 58-59 22.569970110358963 27.02041862773705 28.05105062926151 22.358560632642472 60-61 22.44257985476147 26.982121751195663 28.50636968972271 22.068928704320154 62-63 22.341279800547515 27.06298993220253 28.324128945604322 22.271601321645633 64-65 22.096288752251603 27.05095270306218 28.30155468481721 22.551203859869013 66-67 21.9957575646442 27.244880412936833 28.209150471522022 22.550211550896943 68-69 22.040079989492465 26.702799371287462 28.866917003508018 22.390203635712055 70-71 21.601137471478268 26.726502405888088 29.225576729229342 22.446783393404303 72-73 22.111125825610532 26.906357773115364 28.45816929748049 22.524347103793612 74-75 22.07648254202845 27.297776184797122 27.86765464494322 22.758086628231208 76-77 21.48337644595439 27.29156113491963 28.341926148573354 22.883136270552622 78-79 22.096508134591694 27.216673861598533 27.918166762455137 22.768651241354636 80-81 22.005486590292445 27.35413484119565 27.83672471079845 22.803653857713456 82-83 22.11510841818118 27.400763557216962 27.73094859452859 22.753179430073263 84-85 21.9668704410498 27.29071729469514 27.6739752655334 23.068436998721666 86-87 21.990461977455585 27.24022620417103 27.7969275200105 22.97238429836289 88-89 21.900748934288615 27.14753798216099 27.6580949156758 23.293618167874587 90-91 21.80306435991849 27.258666931060972 27.757256319510383 23.181012389510155 92-93 22.012628045772434 27.172252569206556 27.748795371351743 23.06632401366927 94-95 21.720750688481427 26.990857353839004 27.857517705536683 23.430874252142882 96-97 21.621806551607058 26.879420675998954 27.94694022481462 23.55183254757937 98-99 21.572755796612373 26.93441386242913 27.635150063856408 23.85768027710209 100-101 21.386679325482724 26.75294285515381 27.914036826997275 23.94634099236619 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2659.0 1 1871.5 2 1152.5 3 1272.5 4 1180.5 5 1129.5 6 1649.0 7 2976.0 8 4435.5 9 5192.5 10 4891.5 11 4134.0 12 3923.5 13 3904.5 14 4017.0 15 4343.0 16 4525.5 17 4761.0 18 5077.0 19 5519.5 20 6234.0 21 7137.5 22 8254.0 23 9727.5 24 11577.5 25 13975.0 26 17593.0 27 21854.0 28 25640.0 29 29350.0 30 33337.0 31 38310.0 32 43991.0 33 49015.0 34 54246.5 35 59368.5 36 65317.5 37 71535.0 38 74766.5 39 76444.5 40 78992.0 41 83860.5 42 89450.5 43 94003.0 44 98368.0 45 102162.0 46 104713.5 47 108654.5 48 116486.5 49 118251.5 50 112712.0 51 108258.0 52 104482.0 53 102316.5 54 98818.5 55 90694.5 56 81931.5 57 73802.5 58 65759.0 59 57645.0 60 48717.0 61 39489.5 62 31563.5 63 24708.0 64 18557.0 65 13998.5 66 10493.5 67 8032.5 68 5907.0 69 4111.0 70 3021.5 71 2354.0 72 1934.0 73 1581.0 74 1237.5 75 966.0 76 684.5 77 401.5 78 247.0 79 169.5 80 107.5 81 69.5 82 46.5 83 28.5 84 17.0 85 11.5 86 8.0 87 4.5 88 1.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017997838877542843 2 0.018826913988984353 3 5.181719446509456E-4 4 0.09416911474123184 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011537961967561055 12-13 0.0 14-15 0.0 16-17 4.66354750185851E-4 18-19 1.2090678708522063E-4 20-21 0.0 22-23 1.7272398155031518E-4 24-25 0.003627203612556619 26-27 0.003592658816246556 28-29 0.0011917954726971746 30-31 1.7272398155031518E-4 32-33 5.872615372710717E-4 34-35 0.002832673297425169 36-37 0.08795105140542049 38-39 0.09955810296560168 40-41 0.08067937178215223 42-43 0.0841165790150035 44-45 0.08311477992201168 46-47 0.08157753648621385 48-49 0.07577401070612327 50-51 0.07539401794671258 52-53 0.059140691282827916 54-55 0.05053903700162223 56-57 0.02875854292812748 58-59 0.02229866601814569 60-61 0.013161567394134018 62-63 0.002331773750929255 64-65 0.0064253321136717246 66-67 0.15444978430229184 68-69 0.7138509433492977 70-71 0.8729297303571378 72-73 0.35442961014124674 74-75 0.053319893104582294 76-77 0.1952990059389414 78-79 0.013593377348009805 80-81 0.0033853900383861778 82-83 0.0017790570099682466 84-85 1.899963797053467E-4 86-87 0.002141777371223908 88-89 4.145375557207565E-4 90-91 0.004335371936912911 92-93 0.004283554742447816 94-95 0.0757912831042783 96-97 0.03711838363516273 98-99 0.014077004496350687 100-101 0.012660667847638104 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2894792.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.851764271133916 #Duplication Level Percentage of deduplicated Percentage of total 1 76.25753728548973 42.591179963663784 2 13.59930568550115 15.190904307954032 3 4.48468948591543 7.514334599897442 4 1.9593623080234372 4.377353669978796 5 1.055391625495693 2.947274214045715 6 0.6119604216608654 2.0507441528319825 7 0.41119850305362476 1.6076313302835936 8 0.27667544951835377 1.2362249588887284 9 0.19410126098168184 0.9756808085770865 >10 1.0168067368721552 10.382709605434712 >50 0.08091736069002506 3.1070594063886436 >100 0.04779928740547502 4.793020801263428 >500 0.003128374553344181 1.1542349228960982 >1k 9.385123659027129E-4 0.9186354117634511 >5k 6.256749106018085E-5 0.2816570837953977 >10k+ 1.251349821203617E-4 0.8713547623372508 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13367 0.4617602922766126 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11568 0.3996142037148092 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8060 0.2784310582591081 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3292 0.11372146945272751 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3208 0.11081970656268224 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3170 0.10950700430289982 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 3.4544796310063035E-5 0.0 0.0 7 0.0 0.0 3.4544796310063035E-5 0.0 0.0 8 0.0 0.0 3.4544796310063035E-5 3.4544796310063035E-5 0.0 9 0.0 0.0 3.4544796310063035E-5 3.4544796310063035E-5 0.0 10-11 0.0 0.0 3.4544796310063035E-5 3.4544796310063035E-5 0.0 12-13 3.4544796310063035E-5 0.0 3.4544796310063035E-5 3.4544796310063035E-5 0.0 14-15 3.4544796310063035E-5 0.0 3.4544796310063035E-5 6.908959262012607E-5 0.0 16-17 3.4544796310063035E-5 0.0 3.4544796310063035E-5 1.2090678708522062E-4 0.0 18-19 3.4544796310063035E-5 0.0 3.4544796310063035E-5 1.7272398155031518E-4 0.0 20-21 3.4544796310063035E-5 0.0 3.4544796310063035E-5 2.0726877786037822E-4 0.0 22-23 3.4544796310063035E-5 0.0 3.4544796310063035E-5 4.3180995387578794E-4 0.0 24-25 3.4544796310063035E-5 0.0 3.4544796310063035E-5 7.427131206663553E-4 0.0 26-27 3.4544796310063035E-5 0.0 3.4544796310063035E-5 0.0012263402690072377 0.0 28-29 3.4544796310063035E-5 0.0 3.4544796310063035E-5 0.0025563149269446648 0.0 30-31 3.4544796310063035E-5 0.0 3.4544796310063035E-5 0.006166246141346252 0.0 32-33 3.4544796310063035E-5 0.0 3.4544796310063035E-5 0.01431881807052113 0.0 34-35 3.4544796310063035E-5 0.0 3.4544796310063035E-5 0.02784310582591081 0.0 36-37 3.4544796310063035E-5 0.0 3.4544796310063035E-5 0.05116084333520336 0.0 38-39 3.4544796310063035E-5 0.0 3.4544796310063035E-5 0.08266569756998085 0.0 40-41 3.4544796310063035E-5 0.0 3.4544796310063035E-5 0.11674413912985804 0.0 42-43 3.4544796310063035E-5 0.0 3.4544796310063035E-5 0.15153074901409153 0.0 44-45 3.4544796310063035E-5 0.0 3.4544796310063035E-5 0.19505719236477093 0.0 46-47 3.4544796310063035E-5 0.0 3.4544796310063035E-5 0.24920616058079476 0.0 48-49 3.4544796310063035E-5 0.0 3.4544796310063035E-5 0.3080704934931422 0.0 50-51 3.4544796310063035E-5 0.0 3.4544796310063035E-5 0.3701302200641704 0.0 52-53 3.4544796310063035E-5 0.0 3.4544796310063035E-5 0.4340035484414769 0.0 54-55 3.4544796310063035E-5 0.0 3.4544796310063035E-5 0.5059430867571832 0.0 56-57 3.4544796310063035E-5 0.0 3.4544796310063035E-5 0.5906469273094578 0.0 58-59 3.4544796310063035E-5 0.0 3.4544796310063035E-5 0.6747635063244612 0.0 60-61 3.4544796310063035E-5 0.0 3.4544796310063035E-5 0.7592946228951856 0.0 62-63 3.4544796310063035E-5 0.0 3.4544796310063035E-5 0.8485929213566985 0.0 64-65 3.4544796310063035E-5 0.0 3.4544796310063035E-5 0.943660200801992 0.0 66-67 3.4544796310063035E-5 0.0 3.4544796310063035E-5 1.0408865300166643 0.0 68-69 3.4544796310063035E-5 0.0 3.4544796310063035E-5 1.1579588447114681 0.0 70-71 3.4544796310063035E-5 0.0 3.4544796310063035E-5 1.303772429936244 0.0 72-73 3.4544796310063035E-5 0.0 3.4544796310063035E-5 1.4619185074437127 0.0 74-75 3.4544796310063035E-5 0.0 3.4544796310063035E-5 1.6365251803929262 0.0 76-77 3.4544796310063035E-5 0.0 3.4544796310063035E-5 1.836453189037416 0.0 78-79 3.4544796310063035E-5 0.0 3.4544796310063035E-5 2.0689569406022956 0.0 80-81 3.4544796310063035E-5 0.0 3.4544796310063035E-5 2.3237248133890107 0.0 82-83 3.4544796310063035E-5 0.0 3.4544796310063035E-5 2.5828453305107932 0.0 84-85 3.4544796310063035E-5 0.0 3.4544796310063035E-5 2.847959369792372 0.0 86-87 3.4544796310063035E-5 0.0 3.4544796310063035E-5 3.126010435292069 0.0 88-89 3.4544796310063035E-5 0.0 3.4544796310063035E-5 3.4413180636121696 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11135 0.0 32.610043 1 GTATCAA 16085 0.0 29.12569 1 TATCAAC 19950 0.0 23.478985 2 ATCAACG 19900 0.0 23.462376 3 TCAACGC 20070 0.0 23.268064 4 CAACGCA 20355 0.0 22.96627 5 AACGCAG 20845 0.0 22.471897 6 GTACATG 13110 0.0 22.121708 1 TACATGG 13335 0.0 21.531158 2 GAGTACT 12045 0.0 21.015348 12-13 ACATGGG 13325 0.0 20.867018 3 AGTACTT 12500 0.0 20.041819 12-13 ACGCAGA 23480 0.0 19.828875 7 GTACTTT 12830 0.0 19.655638 14-15 CGCAGAG 23655 0.0 19.642096 8 AGAGTAC 19290 0.0 19.034111 10-11 GCAGAGT 24545 0.0 18.466286 9 TAGCGCG 285 4.3221007E-8 18.317255 4 CATGGGA 7420 0.0 18.22861 4 ACTTTTT 15000 0.0 17.365152 16-17 >>END_MODULE