FastQCFastQC Report
Fri 27 May 2016
SRR939387_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939387_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2317536
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120480.5198624746282258No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95190.4107379561741436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62960.27166784032696795No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40090.17298544661226406No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35890.15486275078359085No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24090.10394660536017565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG134000.027.5782382
GTACATG137150.027.4815351
GTATCAA215850.025.4851651
ACATGGG142500.024.912373
GGTATCA158750.024.6882231
GAGTACT121950.022.83962412-13
CATGGGA94750.022.3321344
TATCAAC252650.021.4089742
CAACGCG3850.021.3302235
ATGGGAG37400.021.1826085
AGTACTT126350.020.94489112-13
ATCAACG260550.020.8513283
GTACTTT133550.020.69417814-15
CATGGGG47250.020.6688164
AACGCGG4000.020.6272836
TCAACGC263750.020.5829584
CAACGCA262300.020.3134845
AACGCAG266600.020.080226
AGAGTAC210550.018.58175810-11
ACTTTTT163650.018.34265716-17
Produced by FastQC (version 0.11.5)