FastQCFastQC Report
Fri 27 May 2016
SRR939387_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939387_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2317536
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172500.7443250072490784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153070.6604859644035734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106890.46122260883973326No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64670.2790463664857849No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT56770.24495843861756625No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39950.17238135675130828No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34640.14946909131077143No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32240.13911326512295819No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23480.10131449953743976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA168250.025.1254161
GTATCAA230450.025.0891861
TACATGG152900.023.520872
GTACATG155250.023.4135481
GAGTACT137900.022.09118312-13
ACATGGG158200.021.5311163
ATCAACG275500.020.9272963
TCAACGC276300.020.8324094
TATCAAC277800.020.7917272
GTACTTT146950.020.66620314-15
AGTACTT141450.020.66590712-13
CAACGCA279200.020.5142215
AACGCAG284250.020.1997626
CATGGGA103850.019.9351314
AGAGTAC244950.018.29646510-11
ACTTTTT170900.018.13022616-17
ACGCAGA317000.018.0381577
AGTACAT117650.017.9623032
CGCAGAG318100.017.93118
ATGGGAG38050.017.9288315
Produced by FastQC (version 0.11.5)