##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939387_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2317536 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.01062594065421 31.0 31.0 33.0 30.0 34.0 2 31.26486061057951 31.0 31.0 34.0 30.0 34.0 3 31.2493134950223 31.0 31.0 34.0 28.0 34.0 4 35.18048867417809 35.0 35.0 37.0 33.0 37.0 5 34.82384696505254 35.0 35.0 37.0 32.0 37.0 6 34.79044511066926 35.0 35.0 37.0 32.0 37.0 7 34.66934364773622 35.0 35.0 37.0 30.0 37.0 8 34.68663269955677 35.0 35.0 37.0 31.0 37.0 9 36.02793095770681 37.0 35.0 39.0 30.0 39.0 10-11 35.89545728739489 37.0 35.0 39.0 30.0 39.0 12-13 35.85308124663435 37.0 35.0 39.0 30.0 39.0 14-15 36.678798732792075 38.0 36.0 40.0 31.0 41.0 16-17 36.703082929456116 38.0 35.5 40.0 31.0 41.0 18-19 36.713783086864666 38.5 35.5 40.0 31.0 41.0 20-21 36.63183031461 38.0 35.0 40.0 30.0 41.0 22-23 36.48992917477873 38.0 35.0 40.0 30.0 41.0 24-25 36.2657488384215 38.0 34.5 40.0 29.5 41.0 26-27 35.89604756948759 38.0 34.0 40.0 28.0 41.0 28-29 35.6195795879762 38.0 34.0 40.0 27.0 41.0 30-31 35.384071056501384 38.0 34.0 40.0 27.0 41.0 32-33 35.14524973937837 37.5 34.0 40.0 27.0 41.0 34-35 35.058090144015026 38.0 33.0 40.0 26.5 41.0 36-37 34.99936915758806 38.0 33.0 40.0 25.5 41.0 38-39 34.95824746627453 38.0 33.0 40.0 26.0 41.0 40-41 34.7395345746517 38.0 33.0 40.0 25.0 41.0 42-43 34.413961638567855 37.5 33.0 40.0 24.5 41.0 44-45 34.19030297695484 37.0 33.0 40.0 24.0 41.0 46-47 33.808479781975336 37.0 33.0 40.0 23.5 41.0 48-49 33.4495052072546 37.0 32.0 40.0 21.5 41.0 50-51 33.17718818607349 37.0 32.0 40.0 20.0 41.0 52-53 32.81379167357055 37.0 31.0 40.0 18.0 41.0 54-55 32.60701991252779 36.5 31.0 39.5 17.5 41.0 56-57 32.23659157829695 36.0 31.0 39.0 15.0 40.0 58-59 31.819203671485578 35.5 30.0 39.0 13.0 40.0 60-61 31.53524087651713 35.0 30.0 39.0 11.0 40.0 62-63 31.16839414792262 35.0 29.0 38.5 8.0 40.0 64-65 30.75505687937534 35.0 29.0 38.0 7.5 40.0 66-67 29.087835313022104 33.5 25.5 37.5 4.5 39.5 68-69 25.45330191203071 30.0 15.5 35.5 2.0 39.0 70-71 24.84779222415531 29.5 14.0 35.0 2.0 38.0 72-73 26.677011705535534 31.0 21.5 35.0 2.0 38.0 74-75 27.29422433998868 32.0 23.5 35.0 2.0 38.0 76-77 27.311749202601384 31.5 25.5 34.5 2.0 37.0 78-79 28.12488112374522 33.0 26.0 35.0 2.0 37.0 80-81 27.96774807381633 33.0 26.0 35.0 2.0 37.0 82-83 27.55369344855916 33.0 26.0 35.0 2.0 36.0 84-85 27.222112623061733 33.0 26.0 35.0 2.0 36.0 86-87 26.780839866133686 32.0 25.0 35.0 2.0 35.5 88-89 26.2944103133673 32.0 24.5 34.5 2.0 35.0 90-91 25.841915940032862 32.0 23.5 34.0 2.0 35.0 92-93 25.298040246192507 31.5 21.0 34.0 2.0 35.0 94-95 23.952291571738257 31.0 10.5 34.0 2.0 35.0 96-97 23.175095877690786 30.0 4.5 34.0 2.0 35.0 98-99 22.8696501370421 30.5 2.0 34.0 2.0 35.0 100-101 21.644005530011185 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.40263864813217154 1101 2 0.30700075505239965 1101 3 0.3032479272158817 1101 4 0.18717194287710015 1101 5 0.24423119568025697 1101 6 0.42925622835701205 1101 7 0.48914365996322573 1101 8 0.4025854875102155 1101 9 0.5335686040452217 1101 10-11 0.724850632715345 1101 12-13 0.566325648084586 1101 14-15 0.6382675885016695 1101 16-17 0.6123787770198277 1101 18-19 0.7135712955302651 1101 20-21 0.7815447618711318 1101 22-23 0.7802909839222778 1101 24-25 0.6937641192066195 1101 26-27 0.7994127362124743 1101 28-29 0.6665192798815411 1101 30-31 0.6501934870350681 1101 32-33 0.6336930492910327 1101 34-35 0.7763342540480807 1101 36-37 0.7547221084253906 1101 38-39 0.7911815065758816 1101 40-41 0.8222109586769974 1101 42-43 0.8433472046591319 1101 44-45 0.56425471080194 1101 46-47 0.7004073768984824 1101 48-49 0.7017037424301336 1101 50-51 0.5537710373071434 1101 52-53 0.7214406353609419 1101 54-55 0.5229673058970619 1101 56-57 0.587605771344343 1101 58-59 0.6251476917861964 1101 60-61 0.4219705614368081 1101 62-63 0.3170396042625434 1101 64-65 0.04557624212609923 1101 66-67 -2.8705660163047746 1101 68-69 -7.84507237301986 1101 70-71 -11.306424924212521 1101 72-73 -10.987183907991197 1101 74-75 -9.420094575402885 1101 76-77 -4.581743706634576 1101 78-79 -1.6064879799436014 1101 80-81 -0.8282519030999538 1101 82-83 -0.6799202767125401 1101 84-85 -0.8824067589352715 1101 86-87 -0.8897192586475633 1101 88-89 -0.7653901125201017 1101 90-91 -0.803845899030005 1101 92-93 -1.319710623430577 1101 94-95 -4.803722060047701 1101 96-97 -1.894484936427407 1101 98-99 -0.9957603417669105 1101 100-101 -0.7604864500424462 1102 1 0.4625688572193454 1102 2 0.2654838510433848 1102 3 0.4352097433311357 1102 4 0.24617291975441447 1102 5 0.4135846440021922 1102 6 0.492196019760037 1102 7 0.5080205885400133 1102 8 0.5822004998101349 1102 9 0.7493444167339973 1102 10-11 0.6720071745177378 1102 12-13 0.6257332881295028 1102 14-15 0.5443867314945479 1102 16-17 0.5813612043759377 1102 18-19 0.6492113135682089 1102 20-21 0.7919113130544204 1102 22-23 0.7769506346439741 1102 24-25 0.8453356364145179 1102 26-27 0.8426945705855715 1102 28-29 0.6025845915438879 1102 30-31 0.3926195573357205 1102 32-33 0.34730574377886825 1102 34-35 0.6137505334328566 1102 36-37 0.7202738593294598 1102 38-39 0.6010772416825532 1102 40-41 0.7293475231321978 1102 42-43 0.8663841462589019 1102 44-45 0.8092447595419614 1102 46-47 0.8981300478890475 1102 48-49 0.5869838032448271 1102 50-51 0.6633042267787346 1102 52-53 0.6408209222302759 1102 54-55 0.4844033292901955 1102 56-57 0.6489964097230541 1102 58-59 0.5980292294158218 1102 60-61 0.32832884619478975 1102 62-63 0.31600194890247835 1102 64-65 0.3317362407944735 1102 66-67 -2.7262144882942643 1102 68-69 -7.6318917676918865 1102 70-71 -10.962767976281441 1102 72-73 -9.300593393062169 1102 74-75 -7.589640742056595 1102 76-77 -3.9182175445102025 1102 78-79 -1.0601619747426483 1102 80-81 -0.5961873445967321 1102 82-83 -0.2402459248806359 1102 84-85 -0.25898288412705384 1102 86-87 -0.2961956635558032 1102 88-89 -0.23044739277577264 1102 90-91 -0.1871158557233592 1102 92-93 -0.5255970231513025 1102 94-95 -4.185565488772536 1102 96-97 -1.0317144378473664 1102 98-99 -0.15939454526983354 1102 100-101 -0.10545287475241594 1103 1 0.2870115067317336 1103 2 0.24253895960533 1103 3 0.33412605059326594 1103 4 0.16946651838981097 1103 5 0.4823982447569932 1103 6 0.39307864063646747 1103 7 0.426713979691165 1103 8 0.35191059988545703 1103 9 0.5556940608015779 1103 10-11 0.6429336404396864 1103 12-13 0.6012096860440579 1103 14-15 0.6271656295159858 1103 16-17 0.6580216954862053 1103 18-19 0.6478630573450346 1103 20-21 0.6479257671077363 1103 22-23 0.5760970350693029 1103 24-25 0.6597516369058312 1103 26-27 0.6240931203634332 1103 28-29 0.780423531146468 1103 30-31 0.7497004840183976 1103 32-33 0.7178729205661654 1103 34-35 0.78802186996014 1103 36-37 0.6992893357514589 1103 38-39 0.8699606561290167 1103 40-41 0.916140241795965 1103 42-43 0.9217263989260616 1103 44-45 0.5998495195171287 1103 46-47 0.6780928861723581 1103 48-49 0.5588814592027163 1103 50-51 0.27586224680344174 1103 52-53 0.5021912213965436 1103 54-55 0.6085602363901259 1103 56-57 0.6259636114373635 1103 58-59 0.6586995552551969 1103 60-61 0.36326218450549774 1103 62-63 0.5026666456930968 1103 64-65 0.441204877016073 1103 66-67 -2.694552594287039 1103 68-69 -7.695990535852026 1103 70-71 -11.427049121045354 1103 72-73 -8.90505773629867 1103 74-75 -7.160973762096347 1103 76-77 -3.6997148389662335 1103 78-79 -1.0405587477184 1103 80-81 -0.4574356429068125 1103 82-83 -0.04200416720128075 1103 84-85 -0.19853706695764473 1103 86-87 0.046241848824909226 1103 88-89 0.0851780042350363 1103 90-91 -0.009605588384179242 1103 92-93 0.2793793022100033 1103 94-95 0.9988014374200311 1103 96-97 1.055526635844462 1103 98-99 0.7744576592352139 1103 100-101 0.5044728319192906 1104 1 0.37573078424181006 1104 2 0.1950540367335556 1104 3 0.3752149374617666 1104 4 0.13201371965053 1104 5 0.36110055102725624 1104 6 0.4559825409910445 1104 7 0.5087893198736069 1104 8 0.4033925527902724 1104 9 0.6071005523008068 1104 10-11 0.6725136022543907 1104 12-13 0.6443036036729382 1104 14-15 0.7577772002648402 1104 16-17 0.7599185345927921 1104 18-19 0.826640218905176 1104 20-21 0.7698998859670283 1104 22-23 0.661530559329087 1104 24-25 0.6984066661509161 1104 26-27 0.9912116462900329 1104 28-29 0.9691447785325948 1104 30-31 0.8346712389355133 1104 32-33 0.6555228584393049 1104 34-35 0.7885732837250217 1104 36-37 0.7426125166466946 1104 38-39 0.8265245861832611 1104 40-41 0.8282619498300292 1104 42-43 1.0971061304168046 1104 44-45 0.8215613418040064 1104 46-47 0.7221217372800339 1104 48-49 0.7770097738979942 1104 50-51 0.9538158486460304 1104 52-53 0.9129712923120792 1104 54-55 0.9763004855036783 1104 56-57 0.7010104338757657 1104 58-59 0.781339189161308 1104 60-61 0.598180813471771 1104 62-63 0.7650215567456193 1104 64-65 0.7160278912294835 1104 66-67 -2.6604814666087684 1104 68-69 -7.333962927281551 1104 70-71 -8.75297314444142 1104 72-73 -8.010602482790777 1104 74-75 -6.811706707515629 1104 76-77 -3.267559188452548 1104 78-79 -0.22043206953553351 1104 80-81 0.3807870743099038 1104 82-83 0.49018648649450824 1104 84-85 0.6127935085492204 1104 86-87 0.837055862220069 1104 88-89 0.7957569962646112 1104 90-91 0.7816284631962667 1104 92-93 1.1865273762773434 1104 94-95 2.039272843911533 1104 96-97 2.2452275177428156 1104 98-99 1.787108506281914 1104 100-101 1.4927863149632046 1105 1 0.5039875748202185 1105 2 0.41013011885003436 1105 3 0.5191650656728015 1105 4 0.32537100618299775 1105 5 0.4067016074247576 1105 6 0.5582866544923561 1105 7 0.5399309354861757 1105 8 0.5184139724779584 1105 9 0.7479306227784903 1105 10-11 0.9207700857405072 1105 12-13 0.8090698296621568 1105 14-15 1.0479196910904633 1105 16-17 1.1036946950178077 1105 18-19 0.9684744811927075 1105 20-21 1.1286799618188255 1105 22-23 1.0663196636944008 1105 24-25 1.184556803574722 1105 26-27 1.0988404331219144 1105 28-29 1.0897436085109433 1105 30-31 1.1743693672079658 1105 32-33 1.2865912325391449 1105 34-35 1.3876778025881649 1105 36-37 1.3497253884203033 1105 38-39 1.1389205202812533 1105 40-41 1.2545562670870751 1105 42-43 1.3115320981414058 1105 44-45 1.056464684503652 1105 46-47 1.0425707062176528 1105 48-49 1.1587941336873229 1105 50-51 1.0763462891699263 1105 52-53 1.3223766080797361 1105 54-55 1.3246513998709943 1105 56-57 1.317814984983599 1105 58-59 1.496383026586642 1105 60-61 1.4652327274245067 1105 62-63 1.4810717356268732 1105 64-65 1.5281701670904972 1105 66-67 2.0900264640497923 1105 68-69 -4.878544283708223 1105 70-71 -5.00490030214867 1105 72-73 -1.1229896647583146 1105 74-75 1.283442155475143 1105 76-77 1.3377897451694523 1105 78-79 1.3524674959288703 1105 80-81 1.3238044096700463 1105 82-83 1.4708954090611854 1105 84-85 1.3011163239713923 1105 86-87 1.4455079634991073 1105 88-89 1.513671993116919 1105 90-91 1.3890362971207502 1105 92-93 1.5517769655413964 1105 94-95 2.503869554953038 1105 96-97 2.5284593570721263 1105 98-99 2.348426546418878 1105 100-101 2.189969050234293 1106 1 0.202165050492507 1106 2 0.1865821222183257 1106 3 0.24855550504929624 1106 4 0.12542210837112577 1106 5 0.39570711309866624 1106 6 0.33057945739019345 1106 7 0.2804895203227318 1106 8 0.30498408871286387 1106 9 0.527484872054842 1106 10-11 0.3139862251236778 1106 12-13 0.34994089625330105 1106 14-15 0.4152756554501451 1106 16-17 0.3631299406002384 1106 18-19 0.4602609631290804 1106 20-21 0.3697139053487959 1106 22-23 0.3891661639168049 1106 24-25 0.4918950838085294 1106 26-27 0.506004490406923 1106 28-29 0.8179619779719332 1106 30-31 0.6414188388209752 1106 32-33 0.6990402131136904 1106 34-35 0.8010514679272163 1106 36-37 0.8167330272644691 1106 38-39 0.8719395761608055 1106 40-41 0.8854579227618586 1106 42-43 0.9087398661555355 1106 44-45 0.9448360475356381 1106 46-47 0.8472357521404064 1106 48-49 0.9119076654143257 1106 50-51 0.7898095789590656 1106 52-53 0.9137688688369252 1106 54-55 1.046034174521175 1106 56-57 0.8757393754139429 1106 58-59 1.0833705074534237 1106 60-61 0.9939754510808427 1106 62-63 1.1304320253142883 1106 64-65 0.9905274466242808 1106 66-67 1.5861996685662874 1106 68-69 -5.371122879656042 1106 70-71 -6.101545584947214 1106 72-73 -2.082077969219011 1106 74-75 0.6228177156279564 1106 76-77 0.9085727462583115 1106 78-79 0.7689650759483335 1106 80-81 0.7136316433442822 1106 82-83 0.8779642140344102 1106 84-85 0.8038750295511612 1106 86-87 0.8929077710081792 1106 88-89 0.7041788738550032 1106 90-91 0.5918340365862669 1106 92-93 0.7240050329818395 1106 94-95 1.6951298769630299 1106 96-97 1.702131256947954 1106 98-99 1.5900991168228273 1106 100-101 1.3217106717927933 1107 1 -0.018628999940318636 1107 2 -0.11872146004233741 1107 3 5.577457260130814E-4 1107 4 0.0013202073462821318 1107 5 0.038271231379937376 1107 6 0.0627886996961351 1107 7 0.008976186566592048 1107 8 -0.02495328218801518 1107 9 0.13329873627238698 1107 10-11 0.177172109952771 1107 12-13 0.12482867276096954 1107 14-15 0.19965376297046333 1107 16-17 0.0741138234465879 1107 18-19 0.23799434444801193 1107 20-21 0.1950860946787003 1107 22-23 0.11226749840000849 1107 24-25 0.4164616733887101 1107 26-27 0.3626065666742946 1107 28-29 0.4114898239333016 1107 30-31 0.2889731207620727 1107 32-33 0.24008398490331473 1107 34-35 0.332501432165607 1107 36-37 0.2364314401892713 1107 38-39 0.2553533794455376 1107 40-41 0.20386926658223814 1107 42-43 0.31572568562143744 1107 44-45 0.17277202742053532 1107 46-47 0.09842914614962694 1107 48-49 0.22994963440595484 1107 50-51 0.17282229686290407 1107 52-53 0.39761795634682073 1107 54-55 0.32260921033589085 1107 56-57 0.3382074334809744 1107 58-59 0.038566455906931196 1107 60-61 0.058192657135407444 1107 62-63 0.33199089481641764 1107 64-65 0.3093862278527233 1107 66-67 1.0439151645269078 1107 68-69 -5.508742503541583 1107 70-71 -5.78098604087516 1107 72-73 -2.099269412649967 1107 74-75 0.3588249413855422 1107 76-77 0.3253109260361988 1107 78-79 0.3447422955124999 1107 80-81 0.2859956543862303 1107 82-83 0.3261596408569254 1107 84-85 0.32138016468818265 1107 86-87 0.4278796548243591 1107 88-89 0.35369883071651387 1107 90-91 0.26199430080647446 1107 92-93 0.30536801320325324 1107 94-95 1.0993439058933738 1107 96-97 0.9696294127289988 1107 98-99 0.8207339833183696 1107 100-101 0.5617480204507679 1108 1 0.09107174029636056 1108 2 0.029524349448802667 1108 3 0.10314073072989416 1108 4 -0.052020812194996324 1108 5 0.12589184459386615 1108 6 0.21080460096257525 1108 7 0.27522454970324617 1108 8 0.139650990591619 1108 9 0.2605119496466344 1108 10-11 0.32772093108756195 1108 12-13 0.37534920224022983 1108 14-15 0.2991791380891513 1108 16-17 0.2985652719762655 1108 18-19 0.2502288674793576 1108 20-21 0.3789837818204447 1108 22-23 0.28857744365658533 1108 24-25 0.323653486190409 1108 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82-83 -6.941968876606754 2315 84-85 -4.93117042501656 2315 86-87 -5.684760581374579 2315 88-89 -3.790899922717127 2315 90-91 -3.426949499537585 2315 92-93 -4.651084066005115 2315 94-95 -2.581262528937419 2315 96-97 -1.0726516109641153 2315 98-99 -2.786784944023637 2315 100-101 -2.0247752641617502 2316 1 -0.33197286097466616 2316 2 -0.3624245788737639 2316 3 -0.8320912870414574 2316 4 -0.3620433931274647 2316 5 -0.14645044252452522 2316 6 -2.14297521660864 2316 7 -0.49856427928418157 2316 8 -0.47422292293022394 2316 9 -0.07021488394079967 2316 10-11 0.058774952432052885 2316 12-13 -0.5747765384510259 2316 14-15 -1.0548665686521232 2316 16-17 -1.6787681347057983 2316 18-19 -0.37493498835893746 2316 20-21 -4.130061951116801 2316 22-23 -4.146398115496552 2316 24-25 -2.7202339444557424 2316 26-27 -2.1884791024926997 2316 28-29 -1.5791734212305357 2316 30-31 -0.3721007047412854 2316 32-33 -1.9192330304934515 2316 34-35 -2.586547126855315 2316 36-37 -4.739308788788232 2316 38-39 -2.460181559362205 2316 40-41 -1.8608366157707081 2316 42-43 -3.187551061318949 2316 44-45 -1.0173187740007066 2316 46-47 -0.9604733410197355 2316 48-49 -0.6467973059809395 2316 50-51 -0.1829810983206812 2316 52-53 -2.727036603463681 2316 54-55 -0.7068420332487904 2316 56-57 0.041957926966958325 2316 58-59 0.16300388188403403 2316 60-61 -1.0839707854228209 2316 62-63 -0.6030466101184437 2316 64-65 0.3579649882159295 2316 66-67 1.2443691716331067 2316 68-69 3.8161094506629176 2316 70-71 3.937174243372766 2316 72-73 2.242597938317264 2316 74-75 1.1334597617566438 2316 76-77 0.0843121104025677 2316 78-79 0.34120893585991396 2316 80-81 -0.3232818821317345 2316 82-83 0.16125636410998467 2316 84-85 0.401877598561061 2316 86-87 -0.0813781422542057 2316 88-89 0.3685428891452567 2316 90-91 0.5325035783429932 2316 92-93 0.8405042153351125 2316 94-95 1.3914876090122057 2316 96-97 1.574998540245776 2316 98-99 0.5930395498402774 2316 100-101 0.349331913068216 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 33.0 4 80.0 5 190.0 6 463.0 7 1093.0 8 2423.0 9 4011.0 10 6333.0 11 9508.0 12 12652.0 13 16139.0 14 19445.0 15 24578.0 16 29694.0 17 33391.0 18 30205.0 19 24429.0 20 23340.0 21 23298.0 22 24677.0 23 28517.0 24 33951.0 25 42179.0 26 52713.0 27 65634.0 28 80449.0 29 95839.0 30 113456.0 31 132657.0 32 153165.0 33 178540.0 34 208734.0 35 242999.0 36 261032.0 37 229547.0 38 104928.0 39 7210.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.5585129110362 20.59982477417016 15.459752007975796 21.38191030681784 2 15.789360110794956 22.162314264591576 39.10200498646353 22.946320638149942 3 19.69281167584883 26.18582839705618 30.670505226240287 23.450854700854702 4 12.663751501594797 18.04817702939674 37.41503907598415 31.873032393024314 5 12.67981944593669 39.150424179504675 33.80141728294699 14.36833909161164 6 30.115432942573495 37.45512475318614 17.501562003783327 14.927880300457035 7 27.093085069660187 34.570077875812935 20.559162835010977 17.777674219515898 8 26.26483472101404 34.86297515982491 19.531562832249424 19.340627286911616 9 26.321576018668104 17.203788851607914 20.848521878408793 35.62611325131519 10-11 24.621969194869035 27.75685037902324 26.68920784833547 20.931972577772253 12-13 26.131244563191252 25.407868529334603 27.63782310177706 20.823063805697085 14-15 22.81865308672659 26.42535002692515 27.326867845850078 23.429129040498182 16-17 20.79728106668899 29.858817148369177 27.837308440701758 21.506593344240077 18-19 20.496554170281357 27.76551486368029 31.33852841722684 20.399402548811516 20-21 21.84789010737702 26.42732472217248 31.348480173977954 20.376304996472545 22-23 21.25308948814603 26.697686681026745 31.18657056459964 20.86265326622758 24-25 21.137794623255044 26.884932963285145 31.43983523880535 20.53743717465446 26-27 20.99330557238985 26.87376120511347 31.656148618925062 20.476784603571613 28-29 20.473446121896927 27.371524699866818 31.647661900154418 20.50736727808184 30-31 20.779923807888174 26.99033391062476 31.494631017141177 20.735111264345893 32-33 20.6248156560573 27.14300170518821 31.348406221876424 20.88377641687806 34-35 21.356640580329305 27.003609796725076 31.193131676667505 20.44661794627811 36-37 21.16021915687496 26.780433631353738 31.3874967927314 20.671850419039902 38-39 20.93826917311811 27.172799317144896 31.402371331835266 20.486560177901726 40-41 20.984349516660366 27.121153534589837 31.305027812280606 20.58946913646919 42-43 20.74030189357353 27.28880236096256 30.83216846048446 21.138727284979446 44-45 21.022587750604558 27.3595200953382 30.590250683925913 21.027641470131332 46-47 21.226265542565443 27.628122227434392 29.990747675904306 21.15486455409586 48-49 21.29009308500586 27.69930642502304 29.868885323307275 21.141715166663825 50-51 21.0804582558073 27.98893775923752 29.806972321720714 21.123631663234463 52-53 21.41590130536971 28.233167899413875 29.304583746738196 21.04634704847822 54-55 20.92961649665445 27.923124885574236 29.583831337287425 21.56342728048389 56-57 21.337061205566947 28.179860770274406 29.12294424206478 21.360133782093868 58-59 21.583363776799512 27.79039991817487 29.21787300834457 21.408363296681053 60-61 21.383198622540792 27.89987097108285 29.714086591780987 21.00284381459537 62-63 21.309033543099968 27.837286450548348 29.64330898521508 21.210371021136606 64-65 21.030158668513558 28.06104659943644 29.482978842759806 21.4258158892902 66-67 20.76061586086777 28.015828325414045 29.634778117236117 21.58877769648207 68-69 20.81757129458685 27.78838005683034 29.947010838830472 21.447037809752338 70-71 20.47341559858551 27.831245934557586 30.21447684994501 21.48086161691189 72-73 21.223790490951913 27.805283419896053 29.327065162409493 21.643860926742537 74-75 20.739310397278977 28.369285297127927 29.21595355834667 21.675450747246426 76-77 20.98212156815781 28.202090690074456 28.90326701342474 21.912520728342997 78-79 20.959790585495817 28.25624277721055 28.981758222220687 21.802208415072943 80-81 21.01752217105341 28.384234253185287 28.818928216596166 21.779315359165132 82-83 21.028670104800963 28.40676477086009 28.755950285130417 21.80861483920854 84-85 20.89268516217224 28.341997707910473 28.64484089999034 22.120476229926958 86-87 20.827212414248702 28.3702630882312 28.7655113924067 22.037013105113395 88-89 20.919919301615067 28.27558873855807 28.430689981547076 22.373801978279783 90-91 20.755952889517168 28.258565679631804 28.7105718968209 22.274909534030137 92-93 20.951475418183122 28.41425150555848 28.62745580304255 22.006817273215848 94-95 20.455008383235604 28.239935684560276 28.86378074627879 22.44127518592533 96-97 20.1741132642269 28.150480672234018 29.05372107149352 22.62168499204556 98-99 20.45174864980978 28.206325508210757 28.320818445403418 23.021107396576042 100-101 20.346742535295874 27.995278204872054 28.70721547430048 22.950763785531596 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5528.0 1 3634.5 2 1867.5 3 2024.5 4 1856.5 5 1834.5 6 2605.0 7 4549.5 8 6561.5 9 7551.0 10 6972.0 11 5597.0 12 5042.0 13 4941.5 14 4940.0 15 4963.0 16 5139.0 17 5388.0 18 5756.0 19 6350.0 20 6957.5 21 7689.5 22 8764.5 23 10159.5 24 11609.0 25 13770.0 26 17106.5 27 21065.0 28 24699.0 29 28112.0 30 32345.5 31 37105.0 32 42094.0 33 47229.5 34 52363.5 35 56984.0 36 62307.5 37 67239.5 38 69228.5 39 69468.0 40 70432.0 41 73055.5 42 75146.0 43 76772.0 44 78530.0 45 79843.0 46 81654.5 47 86807.0 48 96152.5 49 94974.5 50 83582.0 51 75520.0 52 69896.0 53 66852.5 54 63874.0 55 58192.5 56 52443.5 57 46739.0 58 41177.0 59 36100.0 60 30436.0 61 24718.5 62 19693.0 63 15233.0 64 11277.0 65 8368.0 66 6237.0 67 4716.0 68 3338.5 69 2211.0 70 1633.0 71 1260.0 72 999.5 73 870.0 74 692.5 75 519.5 76 366.0 77 212.0 78 132.5 79 81.0 80 58.0 81 45.5 82 29.0 83 18.0 84 8.0 85 3.5 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022696519061624072 2 0.019978114687323086 3 0.0 4 0.0 5 1.2944782734766578E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.0991811993427497E-4 18-19 0.0010571572566726042 20-21 4.314927578255527E-5 22-23 0.0 24-25 0.0 26-27 4.962166714993856E-4 28-29 0.017173411761456994 30-31 0.004509099319277025 32-33 0.00847883269127211 34-35 0.009967482705770267 36-37 0.10709650249230217 38-39 0.11003065324551593 40-41 0.12560754180301836 42-43 0.10994435469395081 44-45 0.10396817999806691 46-47 0.10537053146099995 48-49 0.10793791337006199 50-51 0.10586674813249934 52-53 0.08235039283100672 54-55 0.07007442387086976 56-57 0.04025827430512406 58-59 0.017238135675130828 60-61 0.00975173632685749 62-63 0.0017259710313022108 64-65 0.005436808748601964 66-67 0.42176259613658645 68-69 0.809653010783867 70-71 0.8948296811786312 72-73 0.28025454620769646 74-75 0.14698800795327452 76-77 0.015469015368046063 78-79 0.009406542120597048 80-81 0.0018769934965411543 82-83 0.0 84-85 0.0 86-87 2.3732101680405394E-4 88-89 7.551123261947171E-4 90-91 0.003883434820429974 92-93 0.0017475456691934885 94-95 0.22364269638098394 96-97 0.22892848266434698 98-99 0.015641612471176285 100-101 0.008500407329163387 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2317536.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.267801486229395 #Duplication Level Percentage of deduplicated Percentage of total 1 75.07280528220369 39.9896328878678 2 14.026906175924262 14.943649072901973 3 4.839385115360091 7.73350216921244 4 2.128210582710259 4.534603953628107 5 1.1292992072320356 3.007764299469615 6 0.6589267428706045 2.105974735991945 7 0.4223664099106639 1.5748971054300847 8 0.29327756891917894 1.2497801057240627 9 0.21385954750791433 1.0252645130327782 >10 1.0713472753633795 10.54296271585547 >50 0.08531007854672477 3.1400829949452027 >100 0.05372378819910988 5.226379592243712 >500 0.0026184144297477694 0.9177162581265552 >1k 0.0015546835676626203 1.5935267820994945 >5k 1.636509018592232E-4 0.5293158982991318 >10k+ 2.4547635278883477E-4 1.8849469151716285 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17250 0.7443250072490784 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15307 0.6604859644035734 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10689 0.46122260883973326 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6467 0.2790463664857849 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5677 0.24495843861756625 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3995 0.17238135675130828 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3464 0.14946909131077143 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3224 0.13911326512295819 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2348 0.10131449953743976 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.314927578255526E-5 0.0 8 4.314927578255526E-5 0.0 0.0 4.314927578255526E-5 0.0 9 4.314927578255526E-5 0.0 0.0 4.314927578255526E-5 0.0 10-11 8.629855156511053E-5 0.0 0.0 1.5102246523894343E-4 0.0 12-13 1.5102246523894343E-4 0.0 0.0 1.7259710313022105E-4 0.0 14-15 1.7259710313022105E-4 0.0 0.0 2.1574637891277632E-4 0.0 16-17 1.7259710313022105E-4 0.0 0.0 2.8047029258660924E-4 0.0 18-19 1.7259710313022105E-4 0.0 0.0 3.0204493047788686E-4 0.0 20-21 1.7259710313022105E-4 0.0 0.0 5.82515223064496E-4 0.0 22-23 1.7259710313022105E-4 0.0 0.0 9.924333429987711E-4 0.0 24-25 1.7259710313022105E-4 0.0 0.0 0.001574948566063267 0.0 26-27 1.7259710313022105E-4 0.0 0.0 0.0023300608922579846 0.0 28-29 2.3732101680405397E-4 0.0 0.0 0.004789569611863635 0.0 30-31 2.588956546953316E-4 0.0 0.0 0.010442124739378375 0.0 32-33 2.8047029258660924E-4 0.0 0.0 0.020948973392430584 0.0 34-35 3.0204493047788686E-4 0.0 0.0 0.03663373513938942 0.0 36-37 3.0204493047788686E-4 0.0 0.0 0.062199681040553415 0.0 38-39 3.451942062604421E-4 0.0 0.0 0.1005162379354625 0.0 40-41 3.451942062604421E-4 0.0 0.0 0.14241418472032363 0.0 42-43 3.883434820429974E-4 0.0 0.0 0.18517511702083594 0.0 44-45 4.7464203360810794E-4 0.0 0.0 0.23455946315397042 0.0 46-47 4.962166714993856E-4 0.0 0.0 0.2969748905734366 0.0 48-49 5.177913093906632E-4 0.0 0.0 0.3640504397774188 0.0 50-51 5.177913093906632E-4 0.0 0.0 0.43787885064137083 0.0 52-53 5.177913093906632E-4 0.0 0.0 0.5105853803349765 0.0 54-55 6.040898609557737E-4 0.0 0.0 0.5948990652140894 0.0 56-57 6.040898609557737E-4 0.0 0.0 0.6892665313505378 0.0 58-59 6.040898609557737E-4 0.0 0.0 0.7848421772088978 0.0 60-61 6.040898609557737E-4 0.0 0.0 0.8827478839595156 0.0 62-63 6.040898609557737E-4 0.0 0.0 0.9802436725901993 0.0 64-65 6.040898609557737E-4 0.0 0.0 1.0774805655661877 0.0 66-67 6.040898609557737E-4 0.0 0.0 1.1803052897560167 0.0 68-69 6.903884125208842E-4 0.0 0.0 1.3080487207102716 0.0 70-71 7.119630504121619E-4 0.0 0.0 1.476481918727476 0.0 72-73 7.335376883034396E-4 0.0 0.0 1.6661014111539152 0.0 74-75 7.335376883034396E-4 0.0 0.0 1.8649979978736035 0.0 76-77 7.335376883034396E-4 0.0 0.0 2.089309508029217 0.0 78-79 7.335376883034396E-4 0.0 0.0 2.3516786794250444 0.0 80-81 8.629855156511053E-4 4.314927578255526E-5 0.0 2.6390528561368627 0.0 82-83 8.629855156511053E-4 4.314927578255526E-5 0.0 2.9334172155254548 0.0 84-85 8.629855156511053E-4 4.314927578255526E-5 0.0 3.232074927854411 0.0 86-87 8.84560153542383E-4 4.314927578255526E-5 0.0 3.542555541747787 0.0 88-89 9.061347914336605E-4 4.314927578255526E-5 0.0 3.872280732640183 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16825 0.0 25.125416 1 GTATCAA 23045 0.0 25.089186 1 TACATGG 15290 0.0 23.52087 2 GTACATG 15525 0.0 23.413548 1 GAGTACT 13790 0.0 22.091183 12-13 ACATGGG 15820 0.0 21.531116 3 ATCAACG 27550 0.0 20.927296 3 TCAACGC 27630 0.0 20.832409 4 TATCAAC 27780 0.0 20.791727 2 GTACTTT 14695 0.0 20.666203 14-15 AGTACTT 14145 0.0 20.665907 12-13 CAACGCA 27920 0.0 20.514221 5 AACGCAG 28425 0.0 20.199762 6 CATGGGA 10385 0.0 19.935131 4 AGAGTAC 24495 0.0 18.296465 10-11 ACTTTTT 17090 0.0 18.130226 16-17 ACGCAGA 31700 0.0 18.038157 7 AGTACAT 11765 0.0 17.962303 2 CGCAGAG 31810 0.0 17.9311 8 ATGGGAG 3805 0.0 17.928831 5 >>END_MODULE