FastQCFastQC Report
Fri 27 May 2016
SRR939386_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939386_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2312723
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118750.5134639989311302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86970.3760502230487611No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61420.26557439001557903No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40080.17330220696555534No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33440.14459146209900622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA219550.026.8494361
GTACATG136450.024.828661
TACATGG133500.024.4532342
GGTATCA159200.024.0631961
TATCAAC258300.022.6241192
ACATGGG141550.022.577023
GAGTACT119400.022.56344212-13
TCAACGC267950.022.2064514
ATCAACG265650.021.9946613
CAACGCA267000.021.8699175
AACGCAG270750.021.4195966
AGTACTT124600.021.23602912-13
GTACTTT131350.020.93205314-15
CATGGGA91800.020.307314
ACGCAGA294050.019.3929737
CGCAGAG295550.019.196968
ACTTTTT165050.018.80042816-17
AGAGTAC212500.018.76957910-11
GTATAGG7050.018.5650421
GCAGAGT282950.018.5089599
Produced by FastQC (version 0.11.5)