FastQCFastQC Report
Fri 27 May 2016
SRR939386_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939386_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2312723
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170210.7359722716468855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147310.6369547931161665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103520.4476108898471628No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64350.2782434385786798No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54690.23647449348668215No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38360.16586508630735283No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33010.14273218193445564No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31390.13572745201219516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA172400.024.9998761
GTATCAA232150.024.1065141
GTACATG154700.023.4418741
TACATGG151900.023.3694522
GAGTACT136250.022.11224412-13
ACATGGG154750.021.7378653
AGTACTT139950.020.85013812-13
GTACTTT145450.020.71360214-15
CGCATAG2650.019.91200366-67
CATGGGA103000.019.8584214
TATCAAC282150.019.797082
TCAACGC282400.019.7963584
ATCAACG283000.019.7832153
CAACGCA285000.019.633345
AACGCAG289500.019.4100596
GTGTAGC12850.019.2083261
CTCGCGT901.143767E-418.65676968-69
AGAGTAC244350.018.540610-11
ACTTTTT169000.018.38822416-17
TAGCCCT14850.018.2160034
Produced by FastQC (version 0.11.5)