##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939386_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2312723 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.903852731174464 31.0 31.0 31.0 28.0 34.0 2 31.152489078890987 31.0 31.0 34.0 28.0 34.0 3 31.149919380747285 31.0 31.0 34.0 28.0 34.0 4 35.06020392411889 35.0 35.0 37.0 32.0 37.0 5 34.65144507145906 35.0 35.0 37.0 32.0 37.0 6 34.71769079133126 35.0 35.0 37.0 32.0 37.0 7 34.56178150171897 35.0 35.0 37.0 30.0 37.0 8 34.46991490117926 35.0 35.0 37.0 30.0 37.0 9 35.953096847309425 37.0 35.0 39.0 30.0 39.0 10-11 35.75946341174451 37.0 35.0 39.0 30.0 39.0 12-13 35.739802821176596 37.0 35.0 39.0 30.0 39.0 14-15 36.52824095233195 38.0 35.0 40.0 30.0 41.0 16-17 36.46510023033454 38.0 35.0 40.0 30.0 41.0 18-19 36.51272482696804 38.0 35.0 40.0 30.0 41.0 20-21 36.41903505089023 38.0 34.5 40.0 30.0 41.0 22-23 36.255871758096404 38.0 34.0 40.0 30.0 41.0 24-25 36.08597030426904 38.0 34.0 40.0 29.0 41.0 26-27 35.618224707412 38.0 34.0 40.0 27.0 41.0 28-29 35.407604585590235 38.0 34.0 40.0 27.0 41.0 30-31 35.09691519477257 37.5 33.0 40.0 27.0 41.0 32-33 34.815833543403166 37.0 33.0 40.0 26.0 41.0 34-35 35.002548727193016 37.5 33.0 40.0 26.5 41.0 36-37 34.811400241187556 38.0 33.0 40.0 25.0 41.0 38-39 34.61699628533118 37.0 33.0 40.0 25.0 41.0 40-41 34.45223228203291 37.0 33.0 40.0 24.5 41.0 42-43 34.26580031417511 37.0 33.0 40.0 24.0 41.0 44-45 33.839948190942025 37.0 33.0 40.0 23.5 41.0 46-47 33.37679566467753 37.0 32.0 40.0 22.0 41.0 48-49 32.941378842170025 37.0 31.0 40.0 19.5 41.0 50-51 32.67754417628052 37.0 31.0 39.0 18.0 41.0 52-53 32.572201902259806 36.0 31.0 39.0 18.0 40.0 54-55 32.243527434976 36.0 30.5 39.0 15.5 40.0 56-57 31.765686163020817 35.5 30.0 39.0 13.0 40.0 58-59 31.327443234663207 35.0 29.0 39.0 9.0 40.0 60-61 30.95900243998092 35.0 28.5 38.0 8.0 40.0 62-63 30.810005564868774 35.0 28.5 38.0 8.0 40.0 64-65 30.37642445723072 34.0 28.0 38.0 7.0 40.0 66-67 29.533671347584644 34.0 27.0 37.5 3.5 39.5 68-69 26.149206800814454 31.0 17.0 36.0 2.0 39.0 70-71 24.847918665573005 30.0 12.5 35.0 2.0 38.0 72-73 25.857026976425626 31.0 19.0 35.0 2.0 38.0 74-75 26.598181667238144 31.0 21.5 35.0 2.0 38.0 76-77 26.229328155598402 30.5 23.0 34.0 2.0 36.5 78-79 27.317001430780945 32.0 25.5 35.0 2.0 37.0 80-81 27.31774968294949 32.0 26.0 35.0 2.0 36.5 82-83 26.99697391343451 32.0 25.0 35.0 2.0 36.0 84-85 26.63565135124267 32.0 25.0 35.0 2.0 36.0 86-87 26.201274428455115 32.0 24.0 34.0 2.0 35.5 88-89 25.71115801589728 31.5 22.5 34.0 2.0 35.0 90-91 25.116995420549717 31.0 20.0 34.0 2.0 35.0 92-93 24.569092364282277 31.0 18.5 34.0 2.0 35.0 94-95 23.74530002079799 31.0 8.5 34.0 2.0 35.0 96-97 23.09342969305014 30.0 2.0 34.0 2.0 35.0 98-99 22.273160253086946 30.0 2.0 34.0 2.0 35.0 100-101 20.89514935424606 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3231414664243708 1101 2 0.21237475846309195 1101 3 0.3574786570842967 1101 4 0.29876654806993486 1101 5 0.5176763391832395 1101 6 0.3301278678437072 1101 7 0.5359963411170696 1101 8 0.5622716087684694 1101 9 0.6604298189770574 1101 10-11 0.7244165601090558 1101 12-13 0.7198059131670718 1101 14-15 0.7272877797768658 1101 16-17 0.717242704089962 1101 18-19 0.31996560572385135 1101 20-21 0.7093861115947746 1101 22-23 0.7044917090996918 1101 24-25 0.6456187004815774 1101 26-27 0.8437847172974031 1101 28-29 0.8144165813051387 1101 30-31 0.5755095154888537 1101 32-33 0.7334291603622276 1101 34-35 0.7671024358576517 1101 36-37 0.9630714315228914 1101 38-39 0.9360267044894712 1101 40-41 0.9767703042553038 1101 42-43 0.5966527400709083 1101 44-45 0.7951026116330198 1101 46-47 0.8423712755929529 1101 48-49 0.9927687633106004 1101 50-51 0.7307240542346705 1101 52-53 0.5278743219347035 1101 54-55 0.755395159683502 1101 56-57 0.7599990264924195 1101 58-59 0.6446791671680465 1101 60-61 0.6913700664683056 1101 62-63 0.5183149395360509 1101 64-65 0.37115186895043806 1101 66-67 0.335489178248622 1101 68-69 -6.942210776952752 1101 70-71 -7.356606625276129 1101 72-73 -4.2872163704266235 1101 74-75 -2.073280646064841 1101 76-77 0.3185759328553104 1101 78-79 0.5059044013300564 1101 80-81 0.3101451767467758 1101 82-83 0.016573398804826667 1101 84-85 -0.15007747917993086 1101 86-87 -0.07524481319951093 1101 88-89 -0.11278573469865094 1101 90-91 -0.3330093777389962 1101 92-93 -0.11092626517618598 1101 94-95 -0.1375885582982619 1101 96-97 -0.34730331503647704 1101 98-99 -0.24833550870367915 1101 100-101 -0.07944691516543756 1102 1 0.34675224102889146 1102 2 0.33030433436383433 1102 3 0.3114477102557025 1102 4 0.4331487724457972 1102 5 0.5602149785524873 1102 6 0.5540778410914342 1102 7 0.5408098575772868 1102 8 0.6579633569538572 1102 9 0.6297517750225694 1102 10-11 0.6271925501955735 1102 12-13 0.5723541934501384 1102 14-15 0.7356633236556362 1102 16-17 0.750818069788501 1102 18-19 0.5406425265876038 1102 20-21 0.6490004245661041 1102 22-23 0.8710795070342172 1102 24-25 0.7501888595818897 1102 26-27 0.828036839812718 1102 28-29 0.6905333625523014 1102 30-31 0.5904671282774459 1102 32-33 0.893733328669363 1102 34-35 0.8493189879438248 1102 36-37 0.9053433052925399 1102 38-39 1.1379817657168445 1102 40-41 0.8276131270744855 1102 42-43 0.5155980821466883 1102 44-45 0.6513438414300055 1102 46-47 0.7431581962547966 1102 48-49 0.7276202881902165 1102 50-51 0.6066184256571816 1102 52-53 0.47494173009364715 1102 54-55 0.2855454140824847 1102 56-57 0.42806387804253987 1102 58-59 0.3691414716997805 1102 60-61 0.39681554230545046 1102 62-63 0.26677920758374185 1102 64-65 0.13772913002234688 1102 66-67 0.09448557678683756 1102 68-69 -7.080328855432821 1102 70-71 -7.5764973826178625 1102 72-73 -1.3577568799312516 1102 74-75 0.13820680560440124 1102 76-77 0.558770656600224 1102 78-79 0.23144594559245135 1102 80-81 0.09693207721822006 1102 82-83 0.10233525813834277 1102 84-85 -0.20451015430637298 1102 86-87 -0.07338950514455433 1102 88-89 -0.18113148512517085 1102 90-91 -0.16775572327742339 1102 92-93 -0.1392669553848549 1102 94-95 0.15276857193988036 1102 96-97 -0.03704287155444064 1102 98-99 -0.251538210178591 1102 100-101 -0.24303990960298094 1103 1 0.3350048161403656 1103 2 0.3498269895033488 1103 3 0.4447803134061985 1103 4 0.3056126040559022 1103 5 0.4801382606756661 1103 6 0.4247563756631578 1103 7 0.5255964981520904 1103 8 0.5276537107517854 1103 9 0.6435497967138701 1103 10-11 0.5500663794690936 1103 12-13 0.46657017179778393 1103 14-15 0.6641498250089128 1103 16-17 0.8687389471761975 1103 18-19 0.6840924214412709 1103 20-21 0.6405814164464658 1103 22-23 0.6516632151947377 1103 24-25 0.7049217830392607 1103 26-27 0.8211025367965803 1103 28-29 0.7116940611912455 1103 30-31 0.6684244904676078 1103 32-33 0.7933903861298788 1103 34-35 0.9133621076743381 1103 36-37 0.9880985772126252 1103 38-39 1.114098702064375 1103 40-41 1.1065040280227478 1103 42-43 0.7078497469635536 1103 44-45 1.103372045959084 1103 46-47 1.2626601628805787 1103 48-49 1.2725119290570888 1103 50-51 1.0536381844047753 1103 52-53 0.9202090500305573 1103 54-55 0.9333289440783901 1103 56-57 1.262092102043166 1103 58-59 1.392334512570553 1103 60-61 1.3226520884432738 1103 62-63 1.161612190429313 1103 64-65 1.0950286461545176 1103 66-67 0.8798270346212114 1103 68-69 -6.507659842445296 1103 70-71 -7.346507434801595 1103 72-73 -0.29568464537705097 1103 74-75 1.2028730146898248 1103 76-77 1.4140234702380923 1103 78-79 1.075396745872844 1103 80-81 0.7872371962867035 1103 82-83 0.8446306122283396 1103 84-85 0.6439221431409088 1103 86-87 0.7400700768014339 1103 88-89 1.0099950896358152 1103 90-91 0.7834565019304378 1103 92-93 0.7402802582374335 1103 94-95 0.8676619895849953 1103 96-97 0.9777877503666197 1103 98-99 1.0449963420732935 1103 100-101 1.050058523911435 1104 1 0.2617883463165711 1104 2 0.2538135353887583 1104 3 0.2532060959999356 1104 4 0.17803143026084456 1104 5 0.42429604074676064 1104 6 0.203769199116131 1104 7 0.43634473994393375 1104 8 0.5224447802010062 1104 9 0.42048669232980984 1104 10-11 0.3882061187956154 1104 12-13 0.2944162712512082 1104 14-15 0.5187076691017225 1104 16-17 0.5055823663177321 1104 18-19 0.4551909193199535 1104 20-21 0.45382077164273227 1104 22-23 0.38687193074724036 1104 24-25 0.3660205914710133 1104 26-27 0.6562382723773581 1104 28-29 0.5878771489487633 1104 30-31 0.4792905035395023 1104 32-33 0.6451054751869449 1104 34-35 0.3846526706831739 1104 36-37 0.710400229354974 1104 38-39 0.4984923352486419 1104 40-41 0.5074298345051886 1104 42-43 0.3934661231436536 1104 44-45 0.5465402367246739 1104 46-47 0.5973139439836572 1104 48-49 0.6371496091778752 1104 50-51 0.47082323581324914 1104 52-53 0.2078062770305138 1104 54-55 0.5062426246578369 1104 56-57 0.5685942472482246 1104 58-59 0.5453440107167893 1104 60-61 0.6332869783641506 1104 62-63 0.4476040969385089 1104 64-65 0.3198642919137953 1104 66-67 0.38340795041833076 1104 68-69 -6.3183180238465155 1104 70-71 -3.9567239141808734 1104 72-73 0.32579774387494354 1104 74-75 0.8537611964732577 1104 76-77 1.0268909978534282 1104 78-79 0.47609202642171056 1104 80-81 0.21641437398039187 1104 82-83 0.12008581136452534 1104 84-85 0.26193231017068186 1104 86-87 0.22717973547971937 1104 88-89 0.3477054296197615 1104 90-91 0.23348612391568224 1104 92-93 0.20120372362748995 1104 94-95 0.42114878391286226 1104 96-97 0.20654771195359345 1104 98-99 0.4834518899844653 1104 100-101 0.6457871053050077 1105 1 0.07116975153130767 1105 2 0.24677673668529465 1105 3 0.15427055331439377 1105 4 0.18861731376339463 1105 5 0.37335938981011196 1105 6 0.2932509427645371 1105 7 0.31237046805298974 1105 8 0.3679524902920477 1105 9 0.39871015388661135 1105 10-11 0.22630910273193905 1105 12-13 0.30405388505548814 1105 14-15 0.24127741146312331 1105 16-17 0.2667210347480662 1105 18-19 0.35089376502280345 1105 20-21 0.31410933401463126 1105 22-23 0.5245862820032485 1105 24-25 0.4927793442714332 1105 26-27 0.37916387655295836 1105 28-29 0.30809364520692384 1105 30-31 0.26579844004744047 1105 32-33 0.4566081981480039 1105 34-35 0.360008383538883 1105 36-37 0.379857037353446 1105 38-39 0.3454254290567391 1105 40-41 0.4249016217686403 1105 42-43 0.24798999225085794 1105 44-45 0.4239874756302484 1105 46-47 0.4956975954923095 1105 48-49 0.3369349600048892 1105 50-51 -0.11986268820600543 1105 52-53 -0.10108862978105293 1105 54-55 -0.0493953049384217 1105 56-57 0.008748538444184106 1105 58-59 0.12585785789730153 1105 60-61 0.22072128767345944 1105 62-63 -0.14337110445336165 1105 64-65 -0.18351170312886467 1105 66-67 0.11789124323495415 1105 68-69 -3.5096557901842793 1105 70-71 -3.5466081436484345 1105 72-73 0.2044260745866069 1105 74-75 0.4477867696654947 1105 76-77 0.4313649658690615 1105 78-79 0.2421251726631901 1105 80-81 -0.003083099683749424 1105 82-83 -0.08274008000303112 1105 84-85 -0.031208449636743296 1105 86-87 0.006456631423283454 1105 88-89 -0.04666170537237235 1105 90-91 0.10767757727380456 1105 92-93 0.1290004191325167 1105 94-95 0.3066014985322454 1105 96-97 0.04789754392842838 1105 98-99 0.20391818961742914 1105 100-101 0.2606652274747958 1106 1 0.046149288058416005 1106 2 0.1274232452433708 1106 3 0.07410221171691589 1106 4 0.12880696480974052 1106 5 0.23289658760234033 1106 6 0.19739439590127716 1106 7 0.1431136922427214 1106 8 0.28399890160099517 1106 9 0.2999432972437148 1106 10-11 0.31300220551268154 1106 12-13 0.20612644997843432 1106 14-15 0.17511949899648727 1106 16-17 0.18345443951937312 1106 18-19 0.2558920349638214 1106 20-21 0.23751505037336784 1106 22-23 0.20716999932838576 1106 24-25 0.2804511116905317 1106 26-27 0.4748844575479225 1106 28-29 0.27121366557132376 1106 30-31 0.2890571395003789 1106 32-33 0.30138875502883167 1106 34-35 0.3243697021639207 1106 36-37 0.3220652175600449 1106 38-39 0.30294280204982726 1106 40-41 0.3355711317275265 1106 42-43 0.32177098660034886 1106 44-45 0.41640059542196894 1106 46-47 0.5080928202246824 1106 48-49 0.6637385274322227 1106 50-51 0.387578851805614 1106 52-53 0.27892141523096825 1106 54-55 0.17826551814284386 1106 56-57 0.27871608650095325 1106 58-59 0.34110417409111093 1106 60-61 0.5464999285330343 1106 62-63 0.012562540544632128 1106 64-65 -0.022000256540433583 1106 66-67 0.11275891277692551 1106 68-69 -3.0995703085038535 1106 70-71 -3.214167480098414 1106 72-73 0.5206645453059338 1106 74-75 1.074753663805705 1106 76-77 1.078345212711131 1106 78-79 0.6697408770258342 1106 80-81 0.41007624713134305 1106 82-83 0.2805034071251775 1106 84-85 0.3357894941081412 1106 86-87 0.291293922044229 1106 88-89 0.4657982250899302 1106 90-91 0.5559685533776495 1106 92-93 0.30781900987373234 1106 94-95 0.4845181721851972 1106 96-97 0.6744055532631243 1106 98-99 0.7821007740969002 1106 100-101 1.0340669807028426 1107 1 0.10051274479522476 1107 2 0.11811530283440419 1107 3 0.13750158382713806 1107 4 -0.009270901587754565 1107 5 0.18544681260593165 1107 6 0.08191282187122795 1107 7 0.08269734941776363 1107 8 0.020767461064188808 1107 9 0.10215834201354568 1107 10-11 0.034751311709563026 1107 12-13 0.3099996401775229 1107 14-15 0.014944029363682887 1107 16-17 0.13001828115898917 1107 18-19 0.3369332464906165 1107 20-21 0.33544551486480856 1107 22-23 0.40778375931110844 1107 24-25 0.38291676929353713 1107 26-27 0.525653352614107 1107 28-29 0.5469296511351871 1107 30-31 0.4683990519561192 1107 32-33 0.526056176368634 1107 34-35 0.4314180315959746 1107 36-37 0.6157696209184138 1107 38-39 0.48763997238330603 1107 40-41 0.3958804848397577 1107 42-43 0.36660422898700773 1107 44-45 0.5299605821799602 1107 46-47 0.6048180244644641 1107 48-49 0.48966724041427057 1107 50-51 0.5361541488240107 1107 52-53 0.25018671004453097 1107 54-55 0.4578968719171783 1107 56-57 0.6684576591912013 1107 58-59 0.8338657834505305 1107 60-61 0.703220508475809 1107 62-63 0.591832568270906 1107 64-65 0.5426839677126019 1107 66-67 0.8152702767754398 1107 68-69 -2.210745871793307 1107 70-71 -2.2802876103592027 1107 72-73 1.2848524706225604 1107 74-75 1.4059239784204998 1107 76-77 1.0255241638997568 1107 78-79 0.9508117952707309 1107 80-81 0.8894483703600784 1107 82-83 0.8937007995679949 1107 84-85 0.6520745803925792 1107 86-87 0.6941596203618587 1107 88-89 0.5875356892451222 1107 90-91 0.8598373859410273 1107 92-93 0.8171271961802162 1107 94-95 0.8770579811386412 1107 96-97 0.8967772485330094 1107 98-99 0.9443796972535701 1107 100-101 1.1887506911451027 1108 1 -0.01954407939373226 1108 2 0.010659570430028964 1108 3 -0.009912754979392702 1108 4 0.061513756684206555 1108 5 -0.05425196298402568 1108 6 -0.15681100266702686 1108 7 0.0695418671415311 1108 8 0.2532420969464013 1108 9 0.304694553047284 1108 10-11 0.3039808327188567 1108 12-13 0.030400139630827994 1108 14-15 0.07643152228742167 1108 16-17 0.1427451730792697 1108 18-19 0.1708993165880628 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1.1826837732818873 2311 1 -0.27128982261143264 2311 2 -0.2137301634255664 2311 3 -0.17873147275694024 2311 4 -0.05692532930400063 2311 5 0.41597187499684907 2311 6 0.24817850115744022 2311 7 -2.9773692008945964 2311 8 -0.984297213442801 2311 9 -3.1887970138185793 2311 10-11 -1.689316494576829 2311 12-13 -0.9974322191533886 2311 14-15 -2.0831185899130773 2311 16-17 -0.010577409382058534 2311 18-19 0.39203543710506494 2311 20-21 -0.9265506682790345 2311 22-23 0.014361311629762952 2311 24-25 0.03472284364067235 2311 26-27 0.45718482536599225 2311 28-29 0.3753362768059958 2311 30-31 0.13987906239538717 2311 32-33 0.4698713198269644 2311 34-35 0.09182630471324416 2311 36-37 0.35269525746889485 2311 38-39 0.41642298248002874 2311 40-41 0.2148811699165023 2311 42-43 0.367233847138273 2311 44-45 0.3677123886620919 2311 46-47 0.43008920067599377 2311 48-49 0.7901202881902165 2311 50-51 0.44523345461290376 2311 52-53 0.7017105980181668 2311 54-55 0.6590941250856517 2311 56-57 0.6237376435322979 2311 58-59 0.8628327040305628 2311 60-61 1.226896026768987 2311 62-63 1.0367275231700184 2311 64-65 1.194253119685463 2311 66-67 1.2312566241745948 2311 68-69 3.7534458192044546 2311 70-71 4.900778988887215 2311 72-73 3.5679510564284236 2311 74-75 2.6067330214335307 2311 76-77 2.310577665131266 2311 78-79 1.4757914060818962 2311 80-81 1.07554375914113 2311 82-83 1.0211992907680205 2311 84-85 0.9596618053424208 2311 86-87 0.9643420628235653 2311 88-89 1.1350759375038848 2311 90-91 1.2273362033680755 2311 92-93 1.4473048267933635 2311 94-95 1.5343883023091465 2311 96-97 1.2733667896947019 2311 98-99 1.8724156474713247 2311 100-101 1.9070842419631155 2312 1 -0.13827528068952333 2312 2 -0.012535702342447053 2312 3 -0.10798092032498019 2312 4 0.06336415007606888 2312 5 -1.2609362174392373 2312 6 -0.0772240568919571 2312 7 -0.04569710638468649 2312 8 0.255440639346034 2312 9 -2.3552559627129597 2312 10-11 -0.08060291859289492 2312 12-13 -1.2317943678746133 2312 14-15 -4.1489917595889665 2312 16-17 -1.0148244660145096 2312 18-19 -0.19758450480777867 2312 20-21 0.2051197903275792 2312 22-23 0.19943067392949132 2312 24-25 0.5089364775104173 2312 26-27 0.4619885924737659 2312 28-29 0.24114438786621406 2312 30-31 0.07606788590407376 2312 32-33 0.5457238771213326 2312 34-35 0.3636969475477301 2312 36-37 0.6296193314217859 2312 38-39 0.42361831436706154 2312 40-41 0.2623753435839973 2312 42-43 -0.03268712614868008 2312 44-45 0.5093561946288574 2312 46-47 0.7204801563958654 2312 48-49 0.7070623319116223 2312 50-51 0.930192959714411 2312 52-53 0.8638483712769442 2312 54-55 0.9380172728656149 2312 56-57 0.943088114420771 2312 58-59 1.130699913984909 2312 60-61 1.0915452891024415 2312 62-63 1.1608457712937614 2312 64-65 1.0051993261515584 2312 66-67 1.5815091824756742 2312 68-69 4.223494569122661 2312 70-71 5.260587613369385 2312 72-73 3.146491780353383 2312 74-75 2.4385086812059136 2312 76-77 2.4494216855272057 2312 78-79 1.9055926564153687 2312 80-81 1.6622619861239691 2312 82-83 1.6363766981884815 2312 84-85 1.5058049745652902 2312 86-87 1.6473564789067616 2312 88-89 1.69180818291958 2312 90-91 1.7873537201172063 2312 92-93 1.9293178183823017 2312 94-95 1.6777786238018955 2312 96-97 1.7270206426244812 2312 98-99 1.8701632603108997 2312 100-101 1.8388375024442247 2313 1 -0.294027958493988 2313 2 -0.23648780864821717 2313 3 -0.09127597843903601 2313 4 -0.01196849371896036 2313 5 0.05080580173927984 2313 6 0.00816240595186457 2313 7 -2.594805673605908 2313 8 -3.239103827109094 2313 9 -0.9764329695323752 2313 10-11 -0.17190684720814886 2313 12-13 -0.1350620781984091 2313 14-15 -0.5477786396618924 2313 16-17 -0.26037256131104414 2313 18-19 0.06237026615447405 2313 20-21 -2.3073059236075224 2313 22-23 -0.5066612216580424 2313 24-25 -0.012672659456434587 2313 26-27 -1.3967048493157606 2313 28-29 -1.6786924019239464 2313 30-31 -0.9189306239919759 2313 32-33 -0.911019281548441 2313 34-35 -1.4120792213073443 2313 36-37 -2.3837030744825256 2313 38-39 -2.099075968892933 2313 40-41 -1.2054804844755367 2313 42-43 -0.3203630898953307 2313 44-45 -0.4951710918041883 2313 46-47 -0.6511403754835925 2313 48-49 -2.4257664113171735 2313 50-51 -0.8133189523519277 2313 52-53 -0.6966884167601535 2313 54-55 -0.7160375629599933 2313 56-57 -0.6787497973300134 2313 58-59 -0.7683639501176103 2313 60-61 -0.4397100796544642 2313 62-63 -0.27399285921304894 2313 64-65 -0.16832065000678043 2313 66-67 0.5068540977150526 2313 68-69 1.2264046544968537 2313 70-71 3.8152744285789666 2313 72-73 2.775754792467051 2313 74-75 1.6004961292714093 2313 76-77 1.4936935506114395 2313 78-79 0.8158770033115701 2313 80-81 0.8355935079583787 2313 82-83 0.797595427918683 2313 84-85 0.595226113004717 2313 86-87 0.6840428383198329 2313 88-89 0.315460515370539 2313 90-91 0.8220564882044386 2313 92-93 0.8084239313146533 2313 94-95 1.2749079823559697 2313 96-97 1.2873330385363353 2313 98-99 1.2064411559335007 2313 100-101 1.369998205773289 2314 1 -0.07863140676985125 2314 2 -0.1060321436235867 2314 3 -0.051266126222284214 2314 4 -0.0931877055416237 2314 5 0.053259003709719366 2314 6 -2.5025888255752307 2314 7 -0.611973161290635 2314 8 -0.10303978770022582 2314 9 -0.25689602371956965 2314 10-11 -0.27365807873524517 2314 12-13 -2.0910436052920005 2314 14-15 -0.45985868892297077 2314 16-17 -1.7184932509662119 2314 18-19 -0.34449426586522947 2314 20-21 -0.6101174999622074 2314 22-23 -0.35646294579597537 2314 24-25 -0.23331428507218988 2314 26-27 -1.633669135030047 2314 28-29 -0.654985505372224 2314 30-31 -0.0852372742382741 2314 32-33 0.2920792406190458 2314 34-35 -0.008745477464977114 2314 36-37 -0.4176661286697225 2314 38-39 -0.014114146232842018 2314 40-41 -0.07678467166765301 2314 42-43 -0.08944437068350197 2314 44-45 -3.223884145991377 2314 46-47 -3.5845147597200437 2314 48-49 -3.131629711809783 2314 50-51 -3.511905159248478 2314 52-53 -0.7407894019818286 2314 54-55 -1.228888548181672 2314 56-57 -1.0004628515172023 2314 58-59 -1.614338088048644 2314 60-61 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82-83 -1.0254036545506615 2315 84-85 -0.48954380362426875 2315 86-87 -0.057844419109649436 2315 88-89 -0.06105228642961791 2315 90-91 -0.17200904516644044 2315 92-93 -1.2121836220515192 2315 94-95 -0.2374328049223493 2315 96-97 -0.5933633857382965 2315 98-99 -2.5763642667519804 2315 100-101 -3.7648325270084655 2316 1 -0.4920485022529846 2316 2 -0.36051899325194725 2316 3 -0.8965640107277189 2316 4 -0.3583034859875909 2316 5 -0.245102345632759 2316 6 -0.3486165557067338 2316 7 -0.6238914002843501 2316 8 -0.7902570547099472 2316 9 -4.707369437098642 2316 10-11 -1.353242698517981 2316 12-13 -0.6825771959152149 2316 14-15 -0.35104693935179654 2316 16-17 -0.18028541791876052 2316 18-19 -0.2407146775290414 2316 20-21 -3.403029306708916 2316 22-23 -1.070565718809128 2316 24-25 -0.2758438734083839 2316 26-27 -0.40806235972987537 2316 28-29 -0.016013235503727685 2316 30-31 0.059574475332908605 2316 32-33 -1.2420585524055383 2316 34-35 -0.5244660320676076 2316 36-37 -0.19928791254622524 2316 38-39 -5.151648022733696 2316 40-41 -4.77934084662477 2316 42-43 -3.381039282061437 2316 44-45 -2.2024654781811996 2316 46-47 -2.308085371498205 2316 48-49 -2.5681558696142446 2316 50-51 -0.9656907510152024 2316 52-53 -0.6802362287399717 2316 54-55 0.07580055988007928 2316 56-57 -3.3390173112084582 2316 58-59 -0.5505626449382959 2316 60-61 -0.4176141317940356 2316 62-63 -0.4113225648576524 2316 64-65 -0.00947998947451012 2316 66-67 -0.00873412018286146 2316 68-69 2.7060848095185825 2316 70-71 3.52740127375721 2316 72-73 2.3071409188108873 2316 74-75 1.0950750160103446 2316 76-77 -1.4487157642116664 2316 78-79 -9.547119261306136E-4 2316 80-81 0.0015204134103328215 2316 82-83 0.1316734513916309 2316 84-85 0.41638035455583733 2316 86-87 0.2667155548897533 2316 88-89 0.05929161378849557 2316 90-91 0.15369582046185926 2316 92-93 0.10742758435213773 2316 94-95 0.4096832374630388 2316 96-97 0.7186925486856985 2316 98-99 0.6667007835321002 2316 100-101 0.7180985981157129 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 40.0 4 93.0 5 222.0 6 540.0 7 1257.0 8 2673.0 9 4453.0 10 7070.0 11 10152.0 12 13376.0 13 16911.0 14 20935.0 15 26233.0 16 31240.0 17 35146.0 18 31549.0 19 26580.0 20 25536.0 21 26228.0 22 27479.0 23 30923.0 24 36919.0 25 44301.0 26 55731.0 27 69312.0 28 84159.0 29 100386.0 30 117706.0 31 136659.0 32 157306.0 33 182690.0 34 211268.0 35 236806.0 36 241959.0 37 203865.0 38 89164.0 39 5851.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.33556407255655 20.767888080725257 15.527251087474559 21.369296759243642 2 15.798979014615902 22.220141540196757 39.040841145261204 22.940038299926133 3 19.617497349436768 26.335269873170763 30.666022114258734 23.381210663133736 4 12.649936790133287 18.12398482270725 37.514980154136 31.711098233023467 5 12.629268615394063 39.1998955343982 33.837731539834216 14.333104310373528 6 30.02266159847072 37.60324950285875 17.48047647729538 14.893612421375149 7 26.976425624685707 34.618196818209526 20.584479853402247 17.820897703702517 8 26.241577568952266 34.83962411408543 19.59629406548039 19.322504251481913 9 26.280838647775806 17.201281779097627 20.949720308052456 35.568159265074115 10-11 24.557901894837443 27.842229134031072 26.736202779066083 20.863666192065402 12-13 26.036624360115763 25.44757846054197 27.705004014747985 20.81079316459429 14-15 22.74552551256679 26.45822694719601 27.352173174219306 23.44407436601789 16-17 20.8339729140624 29.863173683894196 27.80014208421019 21.50271131783321 18-19 20.47880798496145 27.855071241794032 31.346865004340124 20.319255768904398 20-21 21.889110801423257 26.48001944028749 31.311921055828996 20.31894870246026 22-23 21.22431136836466 26.745125491817966 31.158650602180423 20.871912537636952 24-25 21.030724469982406 26.904023569645975 31.53259560358734 20.532656356784283 26-27 21.02498731975787 26.91560298929411 31.620978157985657 20.43843153296236 28-29 20.529804760753766 27.43439381052717 31.552500733990257 20.483300694728804 30-31 20.80110034984795 26.991000636047875 31.480651129687608 20.727247884416567 32-33 20.702453270169315 27.173846774496923 31.283745598776154 20.839954356557612 34-35 21.3024162050941 27.058483202534923 31.189729728444206 20.44937086392677 36-37 21.167050357439386 26.77678328572452 31.39141587196622 20.664750484869874 38-39 20.929814838178988 27.21788526290572 31.392547345449795 20.4597525534655 40-41 20.97601643044583 27.128973460641014 31.287345856384196 20.60766425252896 42-43 20.689735755645376 27.367574889429356 30.77793261033262 21.16475674459264 44-45 21.045716589214113 27.38260999203945 30.558153896353573 21.013519522392865 46-47 21.25303845120238 27.633277494061577 29.997697766010806 21.115986288725242 48-49 21.30631212473098 27.697279726239554 29.850031946075283 21.146376202954183 50-51 21.072738574766444 28.003335458743493 29.834851952236473 21.08907401425359 52-53 21.326136612656647 28.246306618633728 29.339053847989288 21.08850292072034 54-55 20.976942313278855 27.927602882681306 29.53803078785315 21.55742401618669 56-57 21.303957810801705 28.182150038428915 29.171008031362494 21.342884119406886 58-59 21.571186318262832 27.776951251632088 29.242576726013645 21.409285704091435 60-61 21.386188495169563 27.908662786172062 29.680853824132292 21.02429489452608 62-63 21.277322544665296 27.86735784363854 29.59155280674077 21.263766804955388 64-65 20.969726872033345 28.069332982786747 29.492787057220532 21.468153087959372 66-67 20.960278960817256 28.102283900748393 29.285754034562196 21.651683103872156 68-69 21.103056776165943 27.793385622395167 29.61693399122903 21.486623610209868 70-71 20.72894404118159 27.787804520038446 29.990287812698952 21.492963626081007 72-73 21.183709645399148 27.81807708204735 29.370154166264374 21.62805910628913 74-75 20.96898851710969 28.361646692112096 28.97138524734667 21.697979543431543 76-77 20.435334038780837 28.225510700310085 29.38018457885975 21.958970682049323 78-79 20.950960459209323 28.228283096051975 28.973912276350706 21.846844168387996 80-81 20.971099350657493 28.342544279347432 28.86757036417577 21.818786005819305 82-83 21.020148239047842 28.406913440598338 28.718996784471372 21.853941535882452 84-85 20.824609831375895 28.351511079171782 28.65139632242236 22.172482767029962 86-87 20.856631575260593 28.36320026877972 28.716535768436067 22.063632387523622 88-89 20.87955255955135 28.302251471217748 28.439860596443133 22.37833537278777 90-91 20.74937225123442 28.237603178028124 28.697512657672284 22.315511913065176 92-93 20.90160806547196 28.371738849838724 28.64497831142856 22.081674773260758 94-95 20.64757277583332 28.161624693555797 28.644600880966912 22.546201649643972 96-97 20.49321934778396 28.081831520084155 28.786412050494143 22.63853708163774 98-99 20.492697072233998 28.14975991337118 28.320535271987545 23.037007742407276 100-101 20.312875012162557 28.01213012313913 28.71946117171368 22.955533692984638 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5425.0 1 3615.0 2 1905.5 3 2011.5 4 1792.5 5 1693.0 6 2523.5 7 4503.0 8 6518.0 9 7379.5 10 6702.5 11 5430.0 12 4836.5 13 4666.5 14 4784.0 15 4983.5 16 5133.5 17 5519.5 18 5843.0 19 6250.5 20 6848.0 21 7630.5 22 8682.5 23 10042.5 24 11724.5 25 13891.5 26 17256.0 27 21187.0 28 24789.5 29 28353.5 30 32460.0 31 37092.0 32 42078.5 33 47273.0 34 52536.0 35 57100.0 36 62056.5 37 67122.5 38 69348.0 39 69495.0 40 70366.0 41 72886.0 42 75148.5 43 76965.5 44 78607.5 45 79730.5 46 80950.0 47 86052.0 48 96050.0 49 94583.5 50 83213.0 51 75292.0 52 69212.5 53 66217.0 54 63472.0 55 58281.0 56 52675.0 57 46806.5 58 41099.0 59 35727.5 60 30315.5 61 24738.0 62 19665.5 63 15242.5 64 11373.0 65 8489.5 66 6221.5 67 4767.0 68 3457.0 69 2284.0 70 1663.5 71 1249.0 72 1004.0 73 866.5 74 710.5 75 552.0 76 386.5 77 243.0 78 149.5 79 89.0 80 55.0 81 40.5 82 22.5 83 10.5 84 7.0 85 6.0 86 4.0 87 2.5 88 1.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020970950693187208 2 0.019327865896607593 3 3.02673515159403E-4 4 0.09365583340503814 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011047583303318209 12-13 0.0 14-15 0.0 16-17 4.3239073594200433E-4 18-19 2.1619536797100213E-5 20-21 0.0 22-23 1.0809768398550108E-4 24-25 0.0037834189394925378 26-27 0.0030267351515940303 28-29 9.512596190724095E-4 30-31 1.729562943768017E-4 32-33 6.05347030318806E-4 34-35 0.002918637467608529 36-37 0.08870495947850218 38-39 0.09877966362595088 40-41 0.08176508816663301 42-43 0.08574308293729944 44-45 0.08446753026627053 46-47 0.08319197759524162 48-49 0.07772223478557527 50-51 0.07767899571198107 52-53 0.06021040997992409 54-55 0.05164907340827242 56-57 0.028624266719360688 58-59 0.021706014944288615 60-61 0.01310143929904273 62-63 0.0021403341429129213 64-65 0.006226426597564862 66-67 0.15486074207762884 68-69 0.7140068222610316 70-71 0.8752669472306022 72-73 0.3558791952170666 74-75 0.05363807079360563 76-77 0.19438125534272804 78-79 0.013555449571781836 80-81 0.0032861695931592325 82-83 0.001707943406970917 84-85 2.1619536797100217E-4 86-87 0.002097095069318721 88-89 5.621079567246056E-4 90-91 0.00406447291785484 92-93 0.004302287822622943 94-95 0.07521436851711165 96-97 0.036796451628664566 98-99 0.01420403567569484 100-101 0.012885243931071728 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2312723.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.1085712536138 #Duplication Level Percentage of deduplicated Percentage of total 1 75.72700921149435 40.97480273743211 2 13.63476146642939 14.755149246646452 3 4.759346201265483 7.725642691553687 4 2.0859977705861565 4.514814360185623 5 1.118241780084209 3.025323251822718 6 0.6324547755165457 2.0532734571435207 7 0.4078191417233241 1.5446557761966877 8 0.27222568157526766 1.178379415086316 9 0.20394525983021713 0.9931667961024078 >10 1.0180831201912643 10.245354461720172 >50 0.0854332199300784 3.228493122334005 >100 0.05031124650697873 4.999891265794762 >500 0.002509349460537418 0.8902173899328717 >1k 0.0014570416222473397 1.505332421874522 >5k 1.6189351358303778E-4 0.5213848860890429 >10k+ 2.4284027037455665E-4 1.8441187200851024 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17021 0.7359722716468855 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14731 0.6369547931161665 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10352 0.4476108898471628 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6435 0.2782434385786798 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5469 0.23647449348668215 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3836 0.16586508630735283 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3301 0.14273218193445564 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3139 0.13572745201219516 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.3239073594200426E-5 0.0 0.0 0.0 0.0 7 8.647814718840085E-5 0.0 0.0 0.0 0.0 8 8.647814718840085E-5 0.0 0.0 0.0 0.0 9 8.647814718840085E-5 0.0 0.0 0.0 0.0 10-11 8.647814718840085E-5 0.0 0.0 2.1619536797100213E-5 0.0 12-13 8.647814718840085E-5 0.0 0.0 4.3239073594200426E-5 0.0 14-15 8.647814718840085E-5 0.0 0.0 1.0809768398550107E-4 0.0 16-17 1.0809768398550108E-4 0.0 0.0 3.675321255507037E-4 0.0 18-19 1.9457583117390195E-4 0.0 0.0 4.756298095362047E-4 0.0 20-21 2.594344415652026E-4 0.0 0.0 6.05347030318806E-4 0.0 22-23 3.2429305195650325E-4 0.0 0.0 0.0010377377662608104 0.0 24-25 3.8915166234780384E-4 0.0 0.0 0.0017728020173622175 0.0 26-27 4.323907359420043E-4 0.0 0.0 0.0025078662684636247 0.0 28-29 4.540102727391045E-4 2.1619536797100213E-5 0.0 0.004302287822622942 0.0 30-31 4.756298095362047E-4 4.3239073594200426E-5 0.0 0.008864010086811088 0.0 32-33 4.756298095362047E-4 4.3239073594200426E-5 0.0 0.019327865896607593 0.0 34-35 4.97249346333305E-4 4.3239073594200426E-5 0.0 0.03519660590567915 0.0 36-37 5.188688831304052E-4 4.3239073594200426E-5 0.0 0.06129138681977911 0.0 38-39 5.621079567246056E-4 4.3239073594200426E-5 0.0 0.09823917520602338 0.0 40-41 5.621079567246056E-4 4.3239073594200426E-5 0.0 0.13890552392136887 0.0 42-43 5.621079567246056E-4 4.3239073594200426E-5 0.0 0.1813014355804824 0.0 44-45 6.053470303188061E-4 4.3239073594200426E-5 0.0 0.2332964215775084 0.0 46-47 6.702056407101066E-4 4.3239073594200426E-5 0.0 0.29579850245792516 0.0 48-49 6.918251775072068E-4 4.3239073594200426E-5 0.0 0.36647276824764574 0.0 50-51 6.918251775072068E-4 4.3239073594200426E-5 0.0 0.4380766741196417 0.0 52-53 6.918251775072068E-4 4.3239073594200426E-5 0.0 0.5124695002384635 0.0 54-55 6.918251775072068E-4 4.3239073594200426E-5 0.0 0.5938886758163429 0.0 56-57 8.215423982898081E-4 4.3239073594200426E-5 0.0 0.6936195990613663 0.0 58-59 8.215423982898081E-4 4.3239073594200426E-5 0.0 0.7959448667220415 0.0 60-61 8.215423982898081E-4 4.3239073594200426E-5 0.0 0.8954379750622967 0.0 62-63 8.431619350869083E-4 4.3239073594200426E-5 0.0 0.9946932684977837 0.0 64-65 8.647814718840086E-4 4.3239073594200426E-5 0.0 1.104152983301502 0.0 66-67 9.08020545478209E-4 4.3239073594200426E-5 0.0 1.2193418753564522 0.0 68-69 9.08020545478209E-4 4.3239073594200426E-5 0.0 1.3536856770136327 0.0 70-71 9.08020545478209E-4 4.3239073594200426E-5 0.0 1.5203506861824785 0.0 72-73 9.08020545478209E-4 4.3239073594200426E-5 0.0 1.7004630472391202 0.0 74-75 9.08020545478209E-4 4.3239073594200426E-5 0.0 1.8962928115472542 0.0 76-77 9.08020545478209E-4 4.3239073594200426E-5 0.0 2.1248113154926034 0.0 78-79 9.08020545478209E-4 4.3239073594200426E-5 0.0 2.389845217088255 0.0 80-81 0.0011674549870434117 4.3239073594200426E-5 0.0 2.685859914914151 0.0 82-83 0.0011674549870434117 4.3239073594200426E-5 0.0 2.980274767017062 0.0 84-85 0.0011890745238405118 4.3239073594200426E-5 0.0 3.2784514185226676 0.0 86-87 0.0012106940606376121 4.3239073594200426E-5 0.0 3.590853725240766 0.0 88-89 0.0012106940606376121 4.3239073594200426E-5 0.0 3.9402902984922967 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17240 0.0 24.999876 1 GTATCAA 23215 0.0 24.106514 1 GTACATG 15470 0.0 23.441874 1 TACATGG 15190 0.0 23.369452 2 GAGTACT 13625 0.0 22.112244 12-13 ACATGGG 15475 0.0 21.737865 3 AGTACTT 13995 0.0 20.850138 12-13 GTACTTT 14545 0.0 20.713602 14-15 CGCATAG 265 0.0 19.912003 66-67 CATGGGA 10300 0.0 19.858421 4 TATCAAC 28215 0.0 19.79708 2 TCAACGC 28240 0.0 19.796358 4 ATCAACG 28300 0.0 19.783215 3 CAACGCA 28500 0.0 19.63334 5 AACGCAG 28950 0.0 19.410059 6 GTGTAGC 1285 0.0 19.208326 1 CTCGCGT 90 1.143767E-4 18.656769 68-69 AGAGTAC 24435 0.0 18.5406 10-11 ACTTTTT 16900 0.0 18.388224 16-17 TAGCCCT 1485 0.0 18.216003 4 >>END_MODULE