##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939385_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 439577 Sequences flagged as poor quality 0 Sequence length 101 %GC 32 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.92512347097323 31.0 31.0 31.0 30.0 34.0 2 31.038873735432016 31.0 31.0 33.0 28.0 34.0 3 30.96632444372658 31.0 31.0 34.0 27.0 34.0 4 34.893101777390534 35.0 35.0 37.0 32.0 37.0 5 34.60152373759318 35.0 35.0 37.0 32.0 37.0 6 34.66823787413809 35.0 35.0 37.0 31.0 37.0 7 34.54899141674837 35.0 35.0 37.0 30.0 37.0 8 34.651696972316564 35.0 35.0 37.0 31.0 37.0 9 35.80943952936573 37.0 35.0 39.0 30.0 39.0 10-11 35.6901657730045 37.0 35.0 39.0 30.0 39.0 12-13 35.61449643634676 37.0 35.0 39.0 30.0 39.0 14-15 36.04043773900818 38.0 34.0 40.0 29.0 41.0 16-17 36.17535039367392 38.0 34.5 40.0 29.5 41.0 18-19 36.45674591709758 38.0 35.0 40.0 30.0 41.0 20-21 36.50766873608037 38.0 35.0 40.0 30.0 41.0 22-23 36.31781235141966 38.0 35.0 40.0 30.0 41.0 24-25 36.027763054026934 38.0 34.5 40.0 29.5 41.0 26-27 35.399365753895225 37.5 33.5 40.0 28.0 41.0 28-29 35.0123960079804 37.0 33.0 40.0 27.0 41.0 30-31 34.58061613778701 36.5 33.0 39.0 27.0 40.5 32-33 33.97013378770955 35.0 33.0 39.0 25.0 40.0 34-35 33.4878496827632 35.0 33.0 39.0 23.5 40.0 36-37 32.966896584671176 35.0 32.5 39.0 21.0 40.0 38-39 32.65644699335952 35.0 32.0 39.0 20.5 40.0 40-41 32.20042791137844 35.0 31.0 39.0 17.5 40.0 42-43 31.71709620840035 35.0 31.0 39.0 15.0 40.0 44-45 31.19661060519545 35.0 30.5 39.0 7.0 40.0 46-47 30.304797566751674 35.0 29.0 38.0 2.0 40.0 48-49 29.55450239662221 35.0 27.0 38.0 2.0 40.0 50-51 29.139862868166443 35.0 26.0 38.0 2.0 40.0 52-53 28.623172959458753 34.0 24.0 38.0 2.0 40.0 54-55 28.46526433366623 34.0 24.0 38.0 2.0 40.0 56-57 28.19175935046647 34.0 23.0 38.0 2.0 40.0 58-59 27.863634812558438 34.0 23.0 38.0 2.0 40.0 60-61 27.571585865502517 33.5 22.0 38.0 2.0 40.0 62-63 27.111172786565266 33.0 22.0 37.0 2.0 40.0 64-65 26.58010200715688 33.0 20.0 36.0 2.0 39.0 66-67 25.163162540351294 32.0 13.5 35.5 2.0 39.0 68-69 22.173867149555143 27.0 7.0 34.5 2.0 37.0 70-71 21.66099795940188 27.0 6.5 34.0 2.0 37.0 72-73 23.119404563933053 29.0 7.0 34.0 2.0 36.5 74-75 23.555664877825727 30.0 6.5 35.0 2.0 36.5 76-77 23.358831331029602 29.5 4.0 33.5 2.0 35.5 78-79 23.995313676557238 31.0 2.0 35.0 2.0 36.0 80-81 23.880731930924505 31.0 2.0 35.0 2.0 36.0 82-83 23.521767517408783 31.0 2.0 35.0 2.0 35.5 84-85 23.212370073957462 31.0 2.0 34.5 2.0 35.0 86-87 22.69333359115695 31.0 2.0 34.0 2.0 35.0 88-89 22.05370845153409 29.5 2.0 34.0 2.0 35.0 90-91 21.511585001035087 29.0 2.0 34.0 2.0 35.0 92-93 21.099272709900653 29.0 2.0 34.0 2.0 35.0 94-95 19.999510893427093 27.0 2.0 34.0 2.0 35.0 96-97 19.22093398881197 26.0 2.0 33.0 2.0 35.0 98-99 18.91315855925128 26.0 2.0 33.0 2.0 35.0 100-101 18.073682199023153 24.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.33007545942939487 1101 2 0.4027453693459151 1101 3 0.31919629912153624 1101 4 0.19756110454802922 1101 5 0.4938864849083444 1101 6 0.4063235841065236 1101 7 0.30939165766348964 1101 8 0.2343570393215728 1101 9 0.8911763941607518 1101 10-11 0.8539787748670804 1101 12-13 0.6068424396742813 1101 14-15 0.9006315858482594 1101 16-17 0.48485075670915734 1101 18-19 0.3938570630389435 1101 20-21 0.5043503840544332 1101 22-23 0.4881741708489926 1101 24-25 0.3912503327892125 1101 26-27 0.6579863777471715 1101 28-29 0.49186293274889437 1101 30-31 0.5109617620416458 1101 32-33 0.5087738177274801 1101 34-35 1.132351724149764 1101 36-37 1.1683055440937409 1101 38-39 1.021766377620466 1101 40-41 1.1329401285711285 1101 42-43 1.0740373449688185 1101 44-45 0.978966670075831 1101 46-47 0.8852198821897481 1101 48-49 0.7665027930624717 1101 50-51 0.5726391880458443 1101 52-53 0.399296688462929 1101 54-55 0.15419742225976307 1101 56-57 -0.169860689613742 1101 58-59 -0.40366166595858033 1101 60-61 -0.7265170387585513 1101 62-63 -0.4924579592330218 1101 64-65 -0.728866898754827 1101 66-67 -2.609444381659788 1101 68-69 -7.176577932515254 1101 70-71 -9.548569672954669 1101 72-73 -9.809622115184887 1101 74-75 -8.31671922400868 1101 76-77 -3.838254502639881 1101 78-79 -1.6968211454517608 1101 80-81 -1.1361314788597845 1101 82-83 -1.0152958912776207 1101 84-85 -0.9525339244413722 1101 86-87 -0.8750445271220038 1101 88-89 -0.8649873903243339 1101 90-91 -0.5389714251176301 1101 92-93 -1.121688291725814 1101 94-95 -3.5423645900419984 1101 96-97 -1.4605275700200124 1101 98-99 -0.8779240807311517 1101 100-101 -0.8153501133234258 1102 1 0.44725972443974626 1102 2 0.5412842631643109 1102 3 0.5569366126382462 1102 4 0.2111961237731137 1102 5 0.5917273603889726 1102 6 0.6523750481704127 1102 7 0.36005714124822674 1102 8 0.39788853887791475 1102 9 0.521404137431972 1102 10-11 0.5130293516195223 1102 12-13 0.6099036641640723 1102 14-15 0.5868043158098075 1102 16-17 0.662756701695848 1102 18-19 0.4072850429265529 1102 20-21 0.6661368377663592 1102 22-23 0.7307059661758188 1102 24-25 0.6267723676901227 1102 26-27 0.7296811426096426 1102 28-29 0.7764532906311814 1102 30-31 -0.018068110777043955 1102 32-33 -0.11611526834172281 1102 34-35 -0.1896388111948042 1102 36-37 0.1622422492105784 1102 38-39 -0.006657165709910373 1102 40-41 0.005063760632644687 1102 42-43 0.275930276054293 1102 44-45 0.22750260618940032 1102 46-47 0.6724573858868759 1102 48-49 0.4159704061946812 1102 50-51 0.3044936689686466 1102 52-53 0.048365011444300166 1102 54-55 0.3272527313399145 1102 56-57 0.20471783744925887 1102 58-59 0.07819281496422192 1102 60-61 0.28173495769220125 1102 62-63 0.32784668436354636 1102 64-65 0.0956376856876453 1102 66-67 -2.3212012583234944 1102 68-69 -6.361952082175119 1102 70-71 -9.681207945655053 1102 72-73 -7.596859618882018 1102 74-75 -5.5091474996667635 1102 76-77 -2.8574647954526604 1102 78-79 -0.9492464634050286 1102 80-81 -0.5261640136128136 1102 82-83 -0.5444144952409466 1102 84-85 -0.4870651282272327 1102 86-87 -0.19063163152898355 1102 88-89 -0.14108913537017997 1102 90-91 -0.5020740574749212 1102 92-93 -0.4852050027580539 1102 94-95 -2.7567538239964513 1102 96-97 -0.6591966028505389 1102 98-99 -0.3181192892493385 1102 100-101 -0.4181451443793236 1103 1 0.2559755755734159 1103 2 0.20999607450602653 1103 3 0.2998417280248056 1103 4 -0.03651886890476419 1103 5 0.36162333574624483 1103 6 0.5812115880886068 1103 7 0.48101103712259174 1103 8 0.3629948359036206 1103 9 1.0044748842885127 1103 10-11 0.6569280033389617 1103 12-13 0.6011928627703469 1103 14-15 0.7051197225663657 1103 16-17 0.5892766734173023 1103 18-19 0.5705494473097303 1103 20-21 0.552322011729899 1103 22-23 0.43437460224105706 1103 24-25 0.6714436296201427 1103 26-27 0.3685679274402176 1103 28-29 0.2746985060025864 1103 30-31 0.6319091653932318 1103 32-33 0.9442101873972959 1103 34-35 1.1222015665257388 1103 36-37 1.1499713811602774 1103 38-39 1.015511192619634 1103 40-41 1.22165341876525 1103 42-43 1.2505679572137147 1103 44-45 1.5246734893889204 1103 46-47 1.4379285266231285 1103 48-49 1.7769889387402529 1103 50-51 1.4412595630251026 1103 52-53 1.422235132133082 1103 54-55 1.316749272268055 1103 56-57 1.4524265179522118 1103 58-59 1.4070318797523846 1103 60-61 1.451481965721289 1103 62-63 1.3512080437535374 1103 64-65 1.4624886678620648 1103 66-67 -1.7030689046397107 1103 68-69 -6.898682628052006 1103 70-71 -9.76136625500212 1103 72-73 -7.353910318270998 1103 74-75 -5.707867390592384 1103 76-77 -2.557604925735948 1103 78-79 -0.13777850400494174 1103 80-81 0.5014328151505048 1103 82-83 0.5937716381731803 1103 84-85 0.46336536210251467 1103 86-87 0.6646933192360542 1103 88-89 0.39581815140455134 1103 90-91 0.389268163400704 1103 92-93 0.28515176136859566 1103 94-95 0.7239246085642712 1103 96-97 0.7354405733348912 1103 98-99 0.4371829376859715 1103 100-101 0.3037846137381308 1104 1 0.6839887559088851 1104 2 0.39782413404935113 1104 3 0.6954463510771447 1104 4 0.16887745186088665 1104 5 0.8441171678128754 1104 6 0.7281615657350216 1104 7 0.5460889435024683 1104 8 0.43628147508366055 1104 9 0.7106701387054173 1104 10-11 0.9609553740607311 1104 12-13 1.0821178044026567 1104 14-15 1.158582872269136 1104 16-17 1.1945020306606935 1104 18-19 0.9452141435493573 1104 20-21 0.8679980834600656 1104 22-23 0.6862705173305557 1104 24-25 0.7184958542157034 1104 26-27 1.0460781521351308 1104 28-29 0.9512876802446399 1104 30-31 1.065957813415352 1104 32-33 1.3246950945885345 1104 34-35 1.4521135596375245 1104 36-37 1.3118692832350263 1104 38-39 1.321076407131251 1104 40-41 1.628507276000363 1104 42-43 2.0351911751363225 1104 44-45 1.7202983962489888 1104 46-47 1.5114242132093239 1104 48-49 1.912795240795127 1104 50-51 1.962518235387794 1104 52-53 2.0655754199147793 1104 54-55 1.5839077177832657 1104 56-57 1.6164583593907906 1104 58-59 1.743345690955671 1104 60-61 1.503193256764952 1104 62-63 1.4923522988824445 1104 64-65 1.520264403460562 1104 66-67 -1.0871055479593004 1104 68-69 -5.66463853353774 1104 70-71 -6.704064498175999 1104 72-73 -5.547697263777927 1104 74-75 -4.846703845148376 1104 76-77 -2.1345074641096 1104 78-79 0.6102552085114112 1104 80-81 1.2413447254712366 1104 82-83 1.2414874330500716 1104 84-85 1.640767958429901 1104 86-87 1.4299972451877387 1104 88-89 1.3486748566518507 1104 90-91 1.3807779410239363 1104 92-93 1.6014943010669036 1104 94-95 2.266203929015319 1104 96-97 2.2160674671802063 1104 98-99 1.521294506907573 1104 100-101 1.4449495666300223 1105 1 0.3589508393686067 1105 2 0.29918479618047655 1105 3 0.6111632986873197 1105 4 0.37133444367091073 1105 5 0.17842838677547945 1105 6 0.4202619254004887 1105 7 0.5788228343894986 1105 8 0.6303188282924808 1105 9 0.9413283698446477 1105 10-11 0.93587195767212 1105 12-13 0.6532167340728918 1105 14-15 0.8004578993523808 1105 16-17 0.9012858413813234 1105 18-19 0.7346516787575794 1105 20-21 0.7681367496645066 1105 22-23 0.9917781656383937 1105 24-25 0.8560353957505669 1105 26-27 1.100409304364625 1105 28-29 0.9598394850719529 1105 30-31 1.0618953269569715 1105 32-33 1.2527165011838477 1105 34-35 1.3130725231076426 1105 36-37 1.428314228418543 1105 38-39 1.4308632078419166 1105 40-41 1.7840690907943113 1105 42-43 1.425036042320098 1105 44-45 1.46114209343666 1105 46-47 1.6728230085466684 1105 48-49 1.0801371829886541 1105 50-51 1.0915493052842287 1105 52-53 1.3293878738732658 1105 54-55 1.397271673236748 1105 56-57 1.3468349248022378 1105 58-59 1.5482271746840581 1105 60-61 1.6495359356226906 1105 62-63 1.4004426052480916 1105 64-65 1.1520492975109171 1105 66-67 1.6280719014492 1105 68-69 -4.256886226045431 1105 70-71 -3.9444011970536685 1105 72-73 -1.0055839041557917 1105 74-75 0.9810445623569315 1105 76-77 1.143030777429594 1105 78-79 0.8138388632325615 1105 80-81 0.9514933583091256 1105 82-83 0.7493589068118247 1105 84-85 0.9065629057800777 1105 86-87 1.3047209961085677 1105 88-89 1.6463673643434902 1105 90-91 1.1111588397542747 1105 92-93 1.3854328546050603 1105 94-95 1.921161245824262 1105 96-97 1.8976356952861089 1105 98-99 1.4490407477534362 1105 100-101 1.2279629565853867 1106 1 0.20309765635597543 1106 2 -0.03748554373645163 1106 3 0.028091331237970252 1106 4 -0.007626309062835901 1106 5 0.471096014138368 1106 6 0.34112316130791953 1106 7 0.4082370922516674 1106 8 0.27367730926221867 1106 9 0.553441618975782 1106 10-11 0.22510650882919236 1106 12-13 0.18816205915553752 1106 14-15 0.4471434973857811 1106 16-17 0.33498084858654664 1106 18-19 0.30440670122091973 1106 20-21 0.3671237802655085 1106 22-23 0.36503407815852995 1106 24-25 0.7092396002093295 1106 26-27 0.7030794747469287 1106 28-29 0.6560673396112477 1106 30-31 0.3118317514716864 1106 32-33 0.10321238996912285 1106 34-35 0.16529342672721015 1106 36-37 0.6043166019033208 1106 38-39 0.1762179590498505 1106 40-41 0.3538198748919612 1106 42-43 0.5065440528312415 1106 44-45 -0.1008609581866331 1106 46-47 -0.29486792982748966 1106 48-49 -0.11705278056140145 1106 50-51 -0.025633029207593694 1106 52-53 0.13279128151927821 1106 54-55 0.2614744366511772 1106 56-57 0.41976448316453485 1106 58-59 0.7908948020469495 1106 60-61 0.28534164893963165 1106 62-63 0.35515566789113606 1106 64-65 0.8465945208727881 1106 66-67 1.2553373079084729 1106 68-69 -5.137042197902687 1106 70-71 -4.202305749058418 1106 72-73 -0.7005360106235408 1106 74-75 0.9508716370369363 1106 76-77 0.9465613360461909 1106 78-79 0.6553578934944966 1106 80-81 0.4078720986668465 1106 82-83 0.30934025800280907 1106 84-85 0.31586188009558214 1106 86-87 0.5588322108241712 1106 88-89 0.7593113331491175 1106 90-91 0.6834653583606993 1106 92-93 0.75180142288513 1106 94-95 1.3313121316426937 1106 96-97 1.170942468194479 1106 98-99 1.0238606172117706 1106 100-101 0.9449799948154478 1107 1 -0.08031415096021632 1107 2 -0.23176297944814905 1107 3 -0.18243223107842255 1107 4 -0.4699507486781087 1107 5 -0.2741613402823333 1107 6 0.06917660410240245 1107 7 -0.10456422772096374 1107 8 -0.1763005570503111 1107 9 -0.07476877178441299 1107 10-11 -0.17404637458663785 1107 12-13 -0.35820600394558966 1107 14-15 -0.02407466028655847 1107 16-17 -0.18546464217767067 1107 18-19 -0.11001842655083038 1107 20-21 -0.6365483997014891 1107 22-23 -0.001065161247481683 1107 24-25 -0.16655218215616685 1107 26-27 -0.1434978523084851 1107 28-29 -0.317454733711628 1107 30-31 -0.2587042861958295 1107 32-33 -0.22741459703235734 1107 34-35 0.05258466581127408 1107 36-37 -0.07321166885203922 1107 38-39 -0.2543828699573538 1107 40-41 -0.2819743220576143 1107 42-43 0.07531543196119017 1107 44-45 0.03951088776290135 1107 46-47 -0.9646152033596245 1107 48-49 -0.44834981464727974 1107 50-51 -0.5351324054400344 1107 52-53 -0.5952199060536607 1107 54-55 -0.7519868696388166 1107 56-57 -0.1902379314195528 1107 58-59 -0.1755746735858743 1107 60-61 -0.48825688621268526 1107 62-63 -0.45567172958964974 1107 64-65 -0.4690338746360858 1107 66-67 0.4276098339658567 1107 68-69 -4.502211822434859 1107 70-71 -4.375316735416014 1107 72-73 -1.6237119935604767 1107 74-75 -0.023006587438896275 1107 76-77 -0.49239797821192965 1107 78-79 -0.7395030667051117 1107 80-81 -0.5397595967736137 1107 82-83 -0.6538755655715853 1107 84-85 -0.5675720609080805 1107 86-87 -0.40963533314137734 1107 88-89 -0.36793523612932333 1107 90-91 -0.3935478011225797 1107 92-93 -0.033984725450817876 1107 94-95 0.4304348327574239 1107 96-97 0.325413473063886 1107 98-99 0.10607921755786265 1107 100-101 -0.1839648319381446 1108 1 -0.06128666681645001 1108 2 -0.024151985790641817 1108 3 -0.09424806382448736 1108 4 0.03989186349008378 1108 5 0.08505876777498145 1108 6 0.1898719294488913 1108 7 0.06687105534222582 1108 8 0.14458269406549817 1108 9 0.12307008186839852 1108 10-11 0.2526390846569626 1108 12-13 0.039985210524697834 1108 14-15 0.07268362019264885 1108 16-17 -0.3025893779070614 1108 18-19 -0.07586291797671407 1108 20-21 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0.7645780890039298 2316 90-91 0.3125798965180344 2316 92-93 0.547680636657347 2316 94-95 1.171420333521521 2316 96-97 1.505938278311941 2316 98-99 0.6347124129807291 2316 100-101 0.8109809357013518 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 19.0 4 43.0 5 176.0 6 363.0 7 670.0 8 1137.0 9 1794.0 10 3025.0 11 4482.0 12 5363.0 13 6667.0 14 8145.0 15 10564.0 16 12260.0 17 13919.0 18 10928.0 19 7180.0 20 6364.0 21 6227.0 22 6463.0 23 7164.0 24 8133.0 25 9253.0 26 10946.0 27 13035.0 28 15519.0 29 17997.0 30 20484.0 31 23486.0 32 27262.0 33 31744.0 34 38223.0 35 42088.0 36 31776.0 37 24609.0 38 11154.0 39 914.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.44348269302533 20.86393796248259 15.552840200602535 13.139739143889543 2 14.594416255204898 27.707342601651913 38.69485084984869 19.0033902932945 3 22.80942815479427 35.00251378029333 30.154216439895627 12.033841625016779 4 10.516018808991372 20.913969566196595 36.90411463748104 31.665896987331006 5 9.059391187437013 46.673961558498284 29.97313326220435 14.293513991860356 6 21.699042488574243 39.830336892057595 19.164787966613357 19.305832652754805 7 21.377369607600034 43.605329669204714 19.10905256644456 15.908248156750696 8 25.527268260168295 32.47758640693212 19.754445751256323 22.240699581643263 9 28.33132761723202 20.956510463468287 21.15442800692484 29.55773391237485 10-11 23.05364020410531 31.243217911765175 23.576301762831086 22.12684012129843 12-13 30.045475536709155 30.474069389435755 25.712218792157003 13.768236281698087 14-15 24.900188135411998 29.717546641430282 31.81626882207213 13.56599640108559 16-17 14.760473729229986 33.78596647678672 33.22554006588167 18.228019728101625 18-19 13.934675167350097 31.481382479568214 40.276632409898255 14.307309943183434 20-21 16.790346173707906 29.089784042386203 39.05163372969923 15.068236054206658 22-23 15.275139509118993 29.27632701437973 38.53215705098311 16.916376425518166 24-25 15.562688675704143 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35.29022627618378 16.347722720870628 50-51 17.20112037163547 33.898756660746 34.97324315707975 13.92687981053878 52-53 14.835764013787262 34.169007799729535 36.33521989955515 14.660008286928052 54-55 13.014514065622881 33.81895660804283 37.0567359742134 16.10979335212088 56-57 14.902644837991724 35.604043493498274 33.735680052432514 15.757631616077482 58-59 14.284137849438636 34.046212985641915 34.33915385026683 17.330495314652616 60-61 14.268261053551196 32.49609821131996 35.84640375663759 17.389236978491247 62-63 17.62657767100725 31.905580883222772 34.7070559544284 15.760785491341577 64-65 16.424227809109542 32.8674518439989 35.79036811728065 14.917952229610906 66-67 14.20047719067411 33.17754394140309 36.45688779851543 16.16509106940737 68-69 15.051520860014836 35.05631349815575 34.06522344815409 15.826942193675322 70-71 15.065163401993233 34.49804211405776 32.667889293370735 17.768905190578277 72-73 14.112963934575815 33.455528434883774 32.63692770946917 19.794579921071243 74-75 14.148755359722228 34.0887446972355 34.03509051722174 17.727409425820532 76-77 15.290255131467731 32.318889876080306 34.70798157529205 17.682873417159914 78-79 13.613473099870887 32.18278095974792 36.474061097618545 17.729684842762644 80-81 13.119911913615285 35.251729821404396 34.62486506653684 17.003493198443472 82-83 12.964395316406454 34.758074239553025 33.273010189341115 19.004520254699404 84-85 13.50536993518769 32.82052973654218 33.26789163218275 20.406208696087376 86-87 14.66638912680728 32.935450370982075 33.92435673881565 18.473803763394994 88-89 15.875902860717739 31.02405732810101 34.40561906386851 18.694420747312744 90-91 13.58789063166504 31.858299369483838 35.626722601325646 18.927087397525483 92-93 12.767562009322726 34.01748522991735 34.31356936649597 18.901383394263956 94-95 12.89031060687243 34.28543091203006 32.69876628217961 20.125492198917904 96-97 12.258473450747337 32.747631332644005 31.930924176424252 23.06297104018441 98-99 13.604541369464068 32.21824056061796 32.25123147105332 21.925986598864657 100-101 14.902245392673553 32.005974366645475 32.77768235070624 20.314097889974736 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12702.0 1 8006.5 2 3683.5 3 4050.5 4 3508.5 5 3092.5 6 4625.0 7 7744.0 8 9698.0 9 9648.0 10 8119.0 11 6152.0 12 5003.5 13 4366.0 14 3931.0 15 3530.5 16 3211.5 17 3101.0 18 3195.0 19 3313.0 20 3473.0 21 3671.0 22 3933.5 23 4305.5 24 4682.0 25 5158.5 26 5726.5 27 6283.5 28 6789.5 29 7297.0 30 7704.5 31 8101.0 32 8600.0 33 9046.0 34 9378.5 35 9577.5 36 9675.5 37 9608.0 38 9328.0 39 9067.5 40 8711.5 41 8228.5 42 7873.0 43 7854.0 44 8604.0 45 9129.0 46 10951.0 47 17135.5 48 31968.0 49 31107.5 50 15740.0 51 9487.0 52 6085.5 53 4406.5 54 3392.0 55 2634.0 56 2086.0 57 1633.0 58 1241.5 59 982.0 60 856.5 61 750.5 62 617.0 63 434.5 64 276.0 65 239.5 66 224.0 67 182.0 68 144.5 69 127.0 70 133.5 71 125.5 72 105.0 73 98.5 74 90.5 75 58.5 76 33.5 77 28.0 78 18.0 79 14.0 80 11.5 81 8.5 82 5.0 83 3.0 84 2.5 85 1.5 86 0.5 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022976634355300663 2 0.019791754345655027 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.274914292604026E-4 18-19 0.0010237114316718119 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.412371438906039E-4 28-29 0.017971822911571806 30-31 0.004777320014468455 32-33 0.007848454309483891 34-35 0.01035086003134832 36-37 0.09748007743808253 38-39 0.10066495744772816 40-41 0.11613437463743553 42-43 0.10237114316718118 44-45 0.0961151288625201 46-47 0.09884502601364493 48-49 0.10134743173550936 50-51 0.09941375458679595 52-53 0.07529966308519327 54-55 0.06347010876365232 56-57 0.03526117153536241 58-59 0.015583162904337578 60-61 0.007962200024114092 62-63 0.001137457146302013 64-65 0.0036398628681664417 66-67 0.41096326695891733 68-69 0.7942863252626957 70-71 0.8878990484033514 72-73 0.274582155117306 74-75 0.1490068861655637 76-77 0.013308248611733554 78-79 0.007848454309483891 80-81 0.0019336771487134224 82-83 0.0 84-85 0.0 86-87 1.137457146302013E-4 88-89 4.549828585208052E-4 90-91 0.0037536085827966433 92-93 0.0013649485755624156 94-95 0.2238515663922362 96-97 0.22442029496538718 98-99 0.014104468614144963 100-101 0.007393471450963085 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 439577.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.191336491882204 #Duplication Level Percentage of deduplicated Percentage of total 1 83.93931857754103 38.77269309314497 2 9.573885628624502 8.844611452131792 3 2.9398819572997668 4.073912302081404 4 1.184682825810324 2.1888833217263417 5 0.6545467608732847 1.5117194840584725 6 0.3895254193951328 1.079561983165327 7 0.23384397696356662 0.7561096078566841 8 0.16320052159239498 0.6030760166820004 9 0.12374871309246234 0.5144506602202185 >10 0.6465894909019374 5.507364699055638 >50 0.06871436648812558 2.2288846208195943 >100 0.05882740728120054 5.707506515457987 >500 0.009886959206924461 2.885379169006886 >1k 0.010381307167270684 10.844604898264881 >5k 0.0014830438810386692 5.502680216423547 >10k+ 0.0014830438810386692 8.978561959904303 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14942 3.399176936008936 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13043 2.967170711843431 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11335 2.5786153506666634 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9066 2.06243729767481 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7808 1.7762530796652236 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7224 1.6433980849771483 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4638 1.0551052489097472 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4252 0.9672935572152319 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3528 0.8025897624307005 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3399 0.7732433680561085 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3294 0.7493567679837663 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2991 0.6804268649178642 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2707 0.6158192990079099 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2672 0.6078570989837958 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2542 0.5782832131799434 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2276 0.5177704929966763 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1871 0.4256364641462133 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1774 0.4035697955079543 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1740 0.39583508691310054 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1382 0.3143931552378764 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1372 0.3121182409452724 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1348 0.3066584466430227 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1252 0.2848192694340241 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1207 0.274582155117306 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1199 0.2727622236832227 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1035 0.23545362928451669 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1013 0.23044881784078786 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 923 0.20997458920735163 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 882 0.20064744060767511 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 842 0.191547783437259 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 697 0.15856152619450062 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 660 0.15014434331186574 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 656 0.1492343775948241 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 643 0.1462769890144389 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 618 0.14058970328292883 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 616 0.140134720424408 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 613 0.1394522461366268 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 588 0.13376496040511673 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 582 0.13240001182955433 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 581 0.13217252040029392 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 560 0.12739520038582547 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 535 0.1217079146543154 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 534 0.121480423225055 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 528 0.12011547464949258 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 526 0.11966049179097178 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 526 0.11966049179097178 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 526 0.11966049179097178 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 483 0.10987836033277447 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 464 0.1055560231768268 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 461 0.1048735488890456 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 445 0.10123368602087915 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 441 0.10032372030383756 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.137457146302013E-4 0.0 12-13 0.0 0.0 0.0 2.274914292604026E-4 0.0 14-15 0.0 0.0 0.0 3.412371438906039E-4 0.0 16-17 0.0 0.0 0.0 6.824742877812078E-4 0.0 18-19 0.0 0.0 0.0 0.0010237114316718119 0.0 20-21 0.0 0.0 0.0 0.0017061857194530195 0.0 22-23 0.0 0.0 0.0 0.0040948457266872474 0.0 24-25 0.0 0.0 0.0 0.008417182882634896 0.0 26-27 0.0 0.0 0.0 0.015128180045816775 0.0 28-29 0.0 0.0 0.0 0.02775395436976912 0.0 30-31 0.0 0.0 0.0 0.05414296016397582 0.0 32-33 0.0 0.0 0.0 0.08883540312618722 0.0 34-35 0.0 0.0 0.0 0.1474144461607409 0.0 36-37 0.0 0.0 0.0 0.22965259783837644 0.0 38-39 0.0 0.0 0.0 0.35192924106584283 0.0 40-41 0.0 0.0 0.0 0.4880828614781938 0.0 42-43 0.0 0.0 0.0 0.5980749675255985 0.0 44-45 0.0 0.0 0.0 0.7181904421750911 0.0 46-47 0.0 0.0 0.0 0.8600313483189521 0.0 48-49 0.0 0.0 0.0 1.0338348002738997 0.0 50-51 0.0 0.0 0.0 1.2220839579868827 0.0 52-53 0.0 0.0 0.0 1.388266447061607 0.0 54-55 0.0 0.0 0.0 1.5629798647335962 0.0 56-57 0.0 0.0 0.0 1.7333709452496375 0.0 58-59 0.0 0.0 0.0 1.9547201059200092 0.0 60-61 0.0 0.0 0.0 2.196770986653078 0.0 62-63 0.0 0.0 0.0 2.417778910179559 0.0 64-65 0.0 0.0 0.0 2.5928335649954386 0.0 66-67 0.0 0.0 0.0 2.771755574108745 0.0 68-69 0.0 0.0 0.0 2.9899198547694716 0.0 70-71 0.0 0.0 0.0 3.280426409935006 0.0 72-73 0.0 0.0 0.0 3.6354267852958637 0.0 74-75 0.0 0.0 0.0 3.9791663349083324 0.0 76-77 0.0 0.0 0.0 4.303341621604407 0.0 78-79 0.0 0.0 0.0 4.65606708267266 0.0 80-81 0.0 0.0 0.0 5.049968492437047 0.0 82-83 0.0 0.0 0.0 5.444211139345326 0.0 84-85 0.0 0.0 0.0 5.941621149423195 0.0 86-87 0.0 0.0 0.0 6.4352775509182685 0.0 88-89 0.0 0.0 0.0 6.8452171064455145 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACGC 30 1.3895606E-6 78.93869 3 TATAACG 40 7.662549E-6 59.210754 2 CGCTAGG 15 0.009807526 48.12135 68-69 TCACGCA 50 0.0016377686 37.89057 4 ATGGGCG 50 0.0016377686 37.89057 5 TCGACGC 45 5.086549E-7 37.48187 66-67 TATCACG 55 0.002613156 34.449894 2 TCTAGCA 70 2.0849265E-4 33.834717 2 GGTATCG 50 1.170105E-6 33.696632 62-63 CGACGCA 45 2.1855027E-5 32.127316 66-67 GTCTTAC 60 0.0039955406 31.589848 1 AGTACTC 230 0.0 30.889051 5 TATCGAC 55 2.4414494E-6 30.666985 64-65 CATGGGT 820 0.0 29.457607 4 AAGTACT 260 0.0 29.146593 4 ATCGACG 50 4.487385E-5 28.914583 64-65 GTATCGA 50 4.5211436E-5 28.882828 62-63 TAACGCA 85 6.5096817E-4 27.860714 4 TGGGGAG 460 0.0 27.800146 6 AGCAACG 185 0.0 27.351633 66-67 >>END_MODULE