##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939384_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 438993 Sequences flagged as poor quality 0 Sequence length 101 %GC 32 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.82275571592258 31.0 30.0 31.0 28.0 34.0 2 30.94257311620003 31.0 31.0 33.0 28.0 34.0 3 30.87371097033438 31.0 30.0 34.0 27.0 34.0 4 34.77329934645883 35.0 35.0 37.0 32.0 37.0 5 34.435590089135815 35.0 35.0 37.0 30.0 37.0 6 34.59914850578483 35.0 35.0 37.0 30.0 37.0 7 34.43632814190659 35.0 35.0 37.0 30.0 37.0 8 34.425608153205175 35.0 35.0 37.0 30.0 37.0 9 35.730050365267786 37.0 35.0 39.0 30.0 39.0 10-11 35.55930618483666 37.0 35.0 39.0 30.0 39.0 12-13 35.487581806543616 37.0 35.0 39.0 30.0 39.0 14-15 35.835811732761115 38.0 34.0 40.0 27.0 41.0 16-17 35.89998473779764 38.0 34.0 40.0 28.5 41.0 18-19 36.251914039631615 38.0 34.0 40.0 30.0 41.0 20-21 36.29477007606044 38.0 34.5 40.0 30.0 41.0 22-23 36.09907447271369 38.0 34.0 40.0 29.5 41.0 24-25 35.84135396236387 38.0 34.0 40.0 29.0 41.0 26-27 35.13606481196739 37.5 33.5 40.0 26.5 41.0 28-29 34.80865070741447 37.0 33.0 39.5 26.5 41.0 30-31 34.34292574141273 36.0 33.0 39.0 25.5 40.0 32-33 33.61905656810018 35.0 33.0 39.0 24.5 40.0 34-35 33.354484012273545 35.0 33.0 39.0 23.5 40.0 36-37 32.82312815921894 35.0 32.0 39.0 21.0 40.0 38-39 32.34944179064358 35.0 31.0 38.5 19.0 40.0 40-41 31.946779333611246 35.0 31.0 38.0 16.5 40.0 42-43 31.585399994988528 35.0 31.0 38.0 15.0 40.0 44-45 30.906342470153284 35.0 30.0 38.0 5.5 40.0 46-47 29.934514901148766 35.0 27.0 38.0 2.0 40.0 48-49 29.12821161157467 35.0 24.5 38.0 2.0 40.0 50-51 28.72562090967282 34.5 24.0 38.0 2.0 40.0 52-53 28.40426612725032 34.0 23.5 38.0 2.0 40.0 54-55 28.119640859877038 34.0 23.0 38.0 2.0 40.0 56-57 27.786995464620166 33.5 22.5 38.0 2.0 40.0 58-59 27.46485707061388 33.0 22.0 38.0 2.0 40.0 60-61 27.116361764310593 33.0 22.0 37.0 2.0 40.0 62-63 26.8373436478486 33.0 21.5 37.0 2.0 39.5 64-65 26.26901795700615 33.0 19.5 36.0 2.0 39.0 66-67 25.52791274576132 32.5 13.0 35.5 2.0 39.0 68-69 22.71047146537644 29.0 6.5 35.0 2.0 38.0 70-71 21.589468396990384 26.5 5.5 34.0 2.0 37.0 72-73 22.35678473233058 28.5 4.5 34.0 2.0 36.5 74-75 22.95222361176602 29.0 2.0 34.0 2.0 36.0 76-77 22.404891421958894 28.5 2.0 33.5 2.0 35.5 78-79 23.290861129904123 30.5 2.0 34.0 2.0 36.0 80-81 23.313028909344794 31.0 2.0 34.0 2.0 36.0 82-83 23.030671331889117 31.0 2.0 34.0 2.0 35.0 84-85 22.70179592840888 30.5 2.0 34.0 2.0 35.0 86-87 22.17130797074213 30.0 2.0 34.0 2.0 35.0 88-89 21.523201964496018 29.0 2.0 34.0 2.0 35.0 90-91 20.868061677521055 29.0 2.0 34.0 2.0 35.0 92-93 20.436028592711047 29.0 2.0 34.0 2.0 35.0 94-95 19.737162095978753 27.0 2.0 33.0 2.0 35.0 96-97 19.065121767317475 26.0 2.0 33.0 2.0 35.0 98-99 18.327477886891135 25.0 2.0 33.0 2.0 35.0 100-101 17.345740137086466 22.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2863857579470448 1101 2 0.18957766305947388 1101 3 0.2752167900866809 1101 4 0.1311962191878422 1101 5 0.4943088247419496 1101 6 0.3685038720610905 1101 7 0.6721828060471324 1101 8 0.7497132371935962 1101 9 0.6009671104948637 1101 10-11 0.6736329963114329 1101 12-13 0.5401853918612289 1101 14-15 0.6558826205034407 1101 16-17 0.7243376407620516 1101 18-19 0.2788403771281551 1101 20-21 0.5844630010542176 1101 22-23 0.7094684509423388 1101 24-25 0.6084181214722975 1101 26-27 0.6431657139849136 1101 28-29 0.9409275806897313 1101 30-31 0.8319797148700303 1101 32-33 1.070182866030045 1101 34-35 1.6104868485746806 1101 36-37 1.7116047808137367 1101 38-39 1.9239439760065622 1101 40-41 1.7478476121408768 1101 42-43 1.6591828901546926 1101 44-45 1.9245845857697041 1101 46-47 1.734310482196193 1101 48-49 1.8252864172566312 1101 50-51 1.5775566123163998 1101 52-53 1.30446071039637 1101 54-55 1.3370250776487609 1101 56-57 1.118597696148825 1101 58-59 1.040628685422071 1101 60-61 1.1296919810029316 1101 62-63 1.110351246321546 1101 64-65 0.7011580213293875 1101 66-67 1.022545880657713 1101 68-69 -4.477855973024173 1101 70-71 -5.060113942190441 1101 72-73 -2.7142422359218656 1101 74-75 -1.0008115999947762 1101 76-77 0.8546725978782064 1101 78-79 1.1481153514794222 1101 80-81 0.8152458255039328 1101 82-83 0.6854649808234292 1101 84-85 0.6984814632995793 1101 86-87 0.46002359261495585 1101 88-89 0.6741001771148412 1101 90-91 0.22011890517915944 1101 92-93 0.46657313900195874 1101 94-95 0.430167174408286 1101 96-97 0.26186665719000146 1101 98-99 0.7017476756829062 1101 100-101 0.5573246385785708 1102 1 0.17813223706532355 1102 2 0.07784892970700241 1102 3 0.19058949466317188 1102 4 0.32496867323587253 1102 5 0.5793058236197908 1102 6 0.3722183044140621 1102 7 0.720922769685707 1102 8 0.857892817384446 1102 9 0.5516008257101177 1102 10-11 0.5279437385889807 1102 12-13 0.4991852787754567 1102 14-15 0.8415629183539437 1102 16-17 0.7322514694110183 1102 18-19 0.4864527887255292 1102 20-21 0.5889364128516732 1102 22-23 0.5244362215000251 1102 24-25 0.6481351460988947 1102 26-27 0.576484259180063 1102 28-29 0.7062159058895858 1102 30-31 0.5860029583436699 1102 32-33 1.1781884681247377 1102 34-35 1.2728671298972642 1102 36-37 1.3163983123081238 1102 38-39 1.3255811098914094 1102 40-41 1.2608394264714526 1102 42-43 1.0986102412511833 1102 44-45 1.1686149623452948 1102 46-47 0.9963106213786723 1102 48-49 1.4243538946612269 1102 50-51 1.4111030688175248 1102 52-53 1.2935174881480513 1102 54-55 1.4212174278318734 1102 56-57 1.3569368142538565 1102 58-59 0.9937394137813698 1102 60-61 0.983474264813399 1102 62-63 0.6764737964924805 1102 64-65 0.42174919890012674 1102 66-67 0.6145063528342654 1102 68-69 -6.218806937297767 1102 70-71 -6.576411340348013 1102 72-73 -0.8001286978163122 1102 74-75 0.6516628034778265 1102 76-77 1.0760010076314188 1102 78-79 1.2125546635700317 1102 80-81 0.8887298238685375 1102 82-83 0.6141600581132032 1102 84-85 0.7800228222424899 1102 86-87 0.7412178652560293 1102 88-89 0.8035050850369281 1102 90-91 0.4893478342569857 1102 92-93 0.582288145482643 1102 94-95 1.2678847557647934 1102 96-97 0.9703511339943738 1102 98-99 0.4528850574865366 1102 100-101 0.3702133708871713 1103 1 0.2863857579470448 1103 2 0.25389301575657086 1103 3 0.28144085647672057 1103 4 0.3946816963662627 1103 5 0.3615287417543982 1103 6 0.5925702621025906 1103 7 0.2842160010678825 1103 8 0.4717049384384069 1103 9 0.6196393096649899 1103 10-11 0.7908529133238815 1103 12-13 0.594127300574911 1103 14-15 0.8581647781797912 1103 16-17 0.8519310017578974 1103 18-19 0.44689016965927664 1103 20-21 0.5222223371537993 1103 22-23 0.5870618119381916 1103 24-25 0.5907832667005124 1103 26-27 1.0228337637774416 1103 28-29 0.51665372176857 1103 30-31 0.42534071072065416 1103 32-33 0.8927969739138604 1103 34-35 1.0057150643423185 1103 36-37 1.060152498656059 1103 38-39 1.050499992604074 1103 40-41 0.6327023839251105 1103 42-43 0.26810405198041565 1103 44-45 0.8509331334875476 1103 46-47 1.2758042581298064 1103 48-49 1.4082739691238508 1103 50-51 1.2248595168807128 1103 52-53 0.9341287601888979 1103 54-55 0.6814234178977259 1103 56-57 0.9329130488459221 1103 58-59 1.118429515297585 1103 60-61 0.762472063990483 1103 62-63 0.46201099735889173 1103 64-65 0.20738208771942723 1103 66-67 0.39495251966186373 1103 68-69 -5.489266761405911 1103 70-71 -6.266545477460149 1103 72-73 -0.13747875044468572 1103 74-75 0.6672381925363418 1103 76-77 0.4501082825255125 1103 78-79 0.6066215755458124 1103 80-81 0.09844084625082061 1103 82-83 0.2342201675454234 1103 84-85 -0.38346874416930277 1103 86-87 -0.3926735028207311 1103 88-89 -0.0966467108519673 1103 90-91 -0.2912918832025859 1103 92-93 -0.24400777386111017 1103 94-95 0.26626675947052547 1103 96-97 0.31165918831033323 1103 98-99 0.07519165908539449 1103 100-101 0.24715866347483484 1104 1 0.4096900174101634 1104 2 0.46074372284438425 1104 3 0.4200893449177414 1104 4 0.45346736831138656 1104 5 0.6957992134285433 1104 6 0.5835347806901083 1104 7 0.5019821116614978 1104 8 0.5906057767820414 1104 9 0.47173516875551513 1104 10-11 0.4560616524211767 1104 12-13 0.3505757720797078 1104 14-15 0.7685128178105813 1104 16-17 0.7305828774459329 1104 18-19 0.5328203923707591 1104 20-21 0.5423933931280587 1104 22-23 0.6595023234124895 1104 24-25 0.3990058088293935 1104 26-27 0.9596785431829744 1104 28-29 0.6007887035621167 1104 30-31 0.7155092262596483 1104 32-33 0.9878769976245891 1104 34-35 0.8755304593799949 1104 36-37 1.7673631007392174 1104 38-39 1.6679654850251069 1104 40-41 2.092101994391701 1104 42-43 2.016385024120307 1104 44-45 1.9185764563768721 1104 46-47 2.131333938881774 1104 48-49 2.03343274632768 1104 50-51 1.6572246621089306 1104 52-53 1.2913012557431394 1104 54-55 1.6071156865064111 1104 56-57 1.5688009309697257 1104 58-59 1.5250896897408097 1104 60-61 1.5258008809944634 1104 62-63 1.5073620008308204 1104 64-65 1.3873719259783819 1104 66-67 1.2087005084839468 1104 68-69 -4.9839911241800685 1104 70-71 -2.5400360355090967 1104 72-73 0.70891806554312 1104 74-75 1.1265996964740204 1104 76-77 1.2674933278299392 1104 78-79 0.7444825290558477 1104 80-81 0.2569131978470587 1104 82-83 0.19101583186924387 1104 84-85 0.45617051402360076 1104 86-87 0.4891920234727749 1104 88-89 0.3278515531839368 1104 90-91 0.8887402956440589 1104 92-93 0.9406268268671454 1104 94-95 1.0476241987117803 1104 96-97 0.6734679782163404 1104 98-99 0.6412006352899873 1104 100-101 0.8465150865419879 1105 1 0.042692220327992914 1105 2 0.13200187987501621 1105 3 0.03868114008794876 1105 4 0.11088455107044126 1105 5 0.36156254523784526 1105 6 -0.04817476667263065 1105 7 0.5103739816477812 1105 8 0.44404523810628405 1105 9 0.34106057712659776 1105 10-11 0.4784981626668312 1105 12-13 0.40292000916353743 1105 14-15 0.5535911668691469 1105 16-17 0.7895213204402367 1105 18-19 0.5129421763185533 1105 20-21 0.5519968679191933 1105 22-23 0.07355943301804757 1105 24-25 0.423079959874741 1105 26-27 0.4880795573564711 1105 28-29 0.20492644827303508 1105 30-31 0.2511154443069472 1105 32-33 0.4348801980901129 1105 34-35 -0.09525594071975974 1105 36-37 -0.041095272426751706 1105 38-39 -0.07207566381732278 1105 40-41 0.03429410545200895 1105 42-43 -0.2249155286873723 1105 44-45 -0.4687442967716393 1105 46-47 0.16719577852597922 1105 48-49 0.012130946585376279 1105 50-51 0.2230087761618833 1105 52-53 -0.37579213965982916 1105 54-55 -0.453173602325208 1105 56-57 -0.5833126232095829 1105 58-59 -0.19244844567629116 1105 60-61 -0.1522987906460891 1105 62-63 -0.8453978811260363 1105 64-65 -0.4945641478401477 1105 66-67 -0.32115526274511197 1105 68-69 -2.286042331628426 1105 70-71 -2.884568227204511 1105 72-73 -0.018433445325992892 1105 74-75 0.49101218244600275 1105 76-77 -0.16861166406498285 1105 78-79 -0.3378878936549867 1105 80-81 -0.6299545023898574 1105 82-83 -0.31224905860833374 1105 84-85 -0.0917658091818474 1105 86-87 -0.11988150410526188 1105 88-89 -0.3122685681843649 1105 90-91 -0.2052577923895278 1105 92-93 -0.27284214524308226 1105 94-95 -0.2970513904648264 1105 96-97 -0.3096298230222807 1105 98-99 -0.37895814105150905 1105 100-101 0.1262731812258906 1106 1 0.15592041039380433 1106 2 0.2096558496785903 1106 3 0.19411531894371592 1106 4 0.20604790465816336 1106 5 0.1331157929529141 1106 6 0.19513876112525708 1106 7 0.18437374600117806 1106 8 0.2864060803745545 1106 9 0.36629580292653685 1106 10-11 0.36288405080201613 1106 12-13 0.1962242794165192 1106 14-15 0.06016402374750385 1106 16-17 0.007168305005386344 1106 18-19 0.1558807735480272 1106 20-21 0.22360860648613112 1106 22-23 0.26216297444976533 1106 24-25 0.5179676911029603 1106 26-27 0.733088899060867 1106 28-29 0.5082478029043287 1106 30-31 0.5690300560791854 1106 32-33 0.1616963600803203 1106 34-35 0.08201131618552893 1106 36-37 0.2047977440180162 1106 38-39 -0.04967661313647653 1106 40-41 0.04520538450807621 1106 42-43 -0.2855335118891382 1106 44-45 -0.036905075078685456 1106 46-47 0.24879472484905207 1106 48-49 0.37486890756903435 1106 50-51 0.3063982158279366 1106 52-53 0.31042758396037584 1106 54-55 0.42862858918814695 1106 56-57 -0.18713598925291208 1106 58-59 0.1343197453994378 1106 60-61 0.3453910311040964 1106 62-63 0.4344913649731694 1106 64-65 0.360891912485382 1106 66-67 0.4988970003038169 1106 68-69 -2.4750182788435886 1106 70-71 -2.0348876125035282 1106 72-73 0.8954419312877633 1106 74-75 0.89403939962801 1106 76-77 0.9973803239507006 1106 78-79 0.7065967135726332 1106 80-81 0.36272362118995005 1106 82-83 0.16543394622242502 1106 84-85 0.27067548956754095 1106 86-87 -0.06694307519478926 1106 88-89 -0.11369002212387258 1106 90-91 0.04662948437740155 1106 92-93 -0.0048698842139778264 1106 94-95 0.03298429316278728 1106 96-97 -0.0885028431700654 1106 98-99 0.2463406251702338 1106 100-101 0.15918585732964274 1107 1 0.05419718197811818 1107 2 0.008073292970305346 1107 3 0.1039799224253386 1107 4 -0.1440575991223909 1107 5 0.03878662115759113 1107 6 0.18010447239657879 1107 7 0.09260304088424931 1107 8 -0.07912935131174947 1107 9 0.5339149342787408 1107 10-11 -0.03399479568998487 1107 12-13 0.11223447811531173 1107 14-15 0.45900348216142817 1107 16-17 -0.072130974052115 1107 18-19 0.2630418426530028 1107 20-21 0.04280589855576267 1107 22-23 -0.035265370899281834 1107 24-25 -0.2454796449265757 1107 26-27 -0.0975511572078176 1107 28-29 0.40795327151695915 1107 30-31 0.31624739386711553 1107 32-33 0.06963991555772253 1107 34-35 0.19706758486849196 1107 36-37 -0.0027194003463222316 1107 38-39 -0.02308966043732852 1107 40-41 -0.009656581379037021 1107 42-43 -4.842767915462787E-4 1107 44-45 0.30898027747977963 1107 46-47 0.4501355020602382 1107 48-49 0.2455256686029692 1107 50-51 0.3136076408323305 1107 52-53 0.18886964480088864 1107 54-55 -0.29279747024564884 1107 56-57 0.15976923318422465 1107 58-59 0.29046094462574246 1107 60-61 0.10226636080340867 1107 62-63 -0.10518684849614601 1107 64-65 -0.4655807444514899 1107 66-67 0.2207934307592403 1107 68-69 -1.5517722791952657 1107 70-71 -2.3798102430644583 1107 72-73 0.5644326117871472 1107 74-75 0.8531303087189173 1107 76-77 0.6176018818898612 1107 78-79 0.35698795675884654 1107 80-81 0.529340466626909 1107 82-83 0.10178439461349242 1107 84-85 0.14284007546520172 1107 86-87 -0.020165336492485864 1107 88-89 -0.08176633652513843 1107 90-91 -0.11538475863297393 1107 92-93 0.3135317335106578 1107 94-95 0.8863779981038782 1107 96-97 0.851170974308392 1107 98-99 1.3804754941697048 1107 100-101 1.220505533784717 1108 1 0.17936304791268753 1108 2 0.14957409370240526 1108 3 0.20242421434938507 1108 4 0.23885249010204035 1108 5 -0.05956615852669245 1108 6 -0.05682071634741703 1108 7 0.11701393084077694 1108 8 0.12400494290010045 1108 9 -0.11744631028771835 1108 10-11 0.33260323679686365 1108 12-13 0.28385424475909815 1108 14-15 0.29682091524310295 1108 16-17 0.1876379575424849 1108 18-19 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-1.0531112233415563 2316 84-85 -0.8924764055592753 2316 86-87 -0.6019601740833629 2316 88-89 -0.7203443395932361 2316 90-91 -1.0292112109773974 2316 92-93 -0.958845375807023 2316 94-95 -0.5501590525197422 2316 96-97 -0.9868000788497469 2316 98-99 -0.3053478900762734 2316 100-101 -0.24994067444579215 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 14.0 4 45.0 5 176.0 6 376.0 7 727.0 8 1230.0 9 1980.0 10 3294.0 11 4604.0 12 5738.0 13 6932.0 14 8285.0 15 10984.0 16 12676.0 17 13971.0 18 10939.0 19 7601.0 20 6710.0 21 6708.0 22 6962.0 23 7471.0 24 8461.0 25 10022.0 26 11592.0 27 13703.0 28 15976.0 29 18490.0 30 21325.0 31 24149.0 32 27831.0 33 32174.0 34 37563.0 35 39201.0 36 29142.0 37 21766.0 38 9444.0 39 728.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.15310935015106 21.133874988038333 15.627634414971908 13.0853812468387 2 14.573403566969533 27.80896572464891 38.65158709183534 18.96604361654622 3 22.724104311695886 35.16237197944382 30.140640376134414 11.972883332725882 4 10.449697450559272 20.90600675777349 37.02753724299257 31.616758548674667 5 9.123835687584995 46.757693175062016 29.91847250411738 14.19999863323561 6 21.740665568699274 39.8448266828856 19.245181586038957 19.169326162376166 7 21.2543252398102 43.717553582858955 19.104177059770887 15.92394411755996 8 25.742323909492864 32.37477590758851 19.80122689883438 22.081673284084257 9 28.189059962231745 20.959331925566012 21.21833377753176 29.633274334670485 10-11 22.91741892419864 31.445143197560576 23.484445497446785 22.152992380793997 12-13 29.894326333221716 30.627481531596175 25.66817694131797 13.810015193864139 14-15 24.904725132291404 29.669038002883873 31.804607362759768 13.621629502064952 16-17 14.829836670539192 33.94872320553999 33.13993485045217 18.081505273468643 18-19 13.924481711553488 31.49754096306775 40.24141615014362 14.33656117523514 20-21 16.824072365618587 29.183267159157435 38.94287608230655 15.049784392917426 22-23 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33.210873017049074 35.08505159633463 17.718522132336933 48-49 15.526700033397125 32.840203300730295 35.241490856824356 16.391605809048226 50-51 17.241143482423745 33.908380066566366 34.850453654310854 14.000022796699039 52-53 14.770227732228294 34.16085996211681 36.23793926380788 14.830973041847024 54-55 12.986058405639541 33.73309721282071 37.056789718273585 16.224054663266166 56-57 14.888339730684613 35.54113160770052 33.67353798021435 15.896990681400514 58-59 14.312438550060552 33.976874885359905 34.38177515844543 17.328911406134115 60-61 14.36853101127538 32.38464325015208 35.72351288728496 17.52331285128759 62-63 17.510894548966814 31.991703513363905 34.588285142181554 15.909116795487732 64-65 16.427157349420096 32.87493194032163 35.611101871954396 15.086808838303872 66-67 13.906533782365448 33.284277487245475 36.55208133687448 16.257107393514588 68-69 15.018711724713931 35.08545900514658 34.04799654905776 15.847832721081723 70-71 15.21456855323364 34.50321628758368 32.435208869094 17.847006290088686 72-73 14.369498449478607 33.42222303504418 32.30331010313567 19.904968412341535 74-75 14.252096281962745 34.133441822620014 33.7919056963713 17.822556199045934 76-77 14.97846524653698 32.407464468227396 34.76156565760313 17.852504627632502 78-79 13.713928999421315 32.18424062370423 36.24756792716768 17.854262449706784 80-81 13.277278572494364 35.218024696139075 34.46843844360335 17.03625828776321 82-83 13.040209847955907 34.77970178901533 33.173153038433014 19.00693532459574 84-85 13.590110981521303 32.76790921019062 33.14194791704632 20.500031891241754 86-87 14.715144998764199 32.85636346448012 33.876782087831856 18.551709448923827 88-89 15.928290346646012 31.12034943649379 34.182588540806506 18.768771676053692 90-91 13.728552162780314 31.93085208168175 35.37378238247542 18.966813373062518 92-93 12.82100932416332 33.982281128194145 34.13775978604314 19.058949761599393 94-95 12.883226488906722 34.281786596875826 32.58838602602566 20.24660088819179 96-97 12.285833110198315 32.81429279492278 31.759082544764684 23.140791550114226 98-99 13.739075358382923 32.15991214719901 32.04872914179479 22.05228335262328 100-101 14.985902978316219 31.984348034106247 32.63229841258992 20.397450574987612 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12550.0 1 8082.0 2 3799.0 3 4002.5 4 3487.5 5 3076.0 6 4582.5 7 7738.5 8 9702.5 9 9430.5 10 7697.0 11 5868.5 12 4955.5 13 4338.0 14 3848.5 15 3407.0 16 3156.5 17 3082.5 18 3145.5 19 3365.0 20 3501.5 21 3665.0 22 3948.5 23 4209.5 24 4724.5 25 5301.0 26 5716.0 27 6236.0 28 6803.5 29 7305.5 30 7850.5 31 8228.0 32 8526.0 33 9045.5 34 9461.5 35 9626.0 36 9630.0 37 9591.0 38 9357.5 39 9040.5 40 8724.5 41 8270.5 42 8013.0 43 8001.0 44 8577.0 45 9191.0 46 10914.0 47 16826.0 48 31778.0 49 31121.0 50 15843.0 51 9639.5 52 6152.5 53 4352.0 54 3307.5 55 2634.0 56 2141.5 57 1685.5 58 1327.5 59 1050.0 60 856.5 61 723.5 62 564.0 63 393.5 64 287.5 65 247.5 66 229.0 67 207.5 68 173.5 69 142.5 70 141.0 71 126.5 72 104.0 73 118.0 74 108.0 75 74.0 76 48.5 77 28.0 78 19.5 79 16.0 80 10.5 81 4.0 82 4.0 83 4.5 84 2.0 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020729259919862048 2 0.01754014300911404 3 2.277940650534291E-4 4 0.08815630317567706 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.008314483374450163 12-13 0.0 14-15 0.0 16-17 6.833821951602873E-4 18-19 0.0 20-21 0.0 22-23 6.833821951602873E-4 24-25 0.003872499105908295 26-27 0.00501146943117544 28-29 0.0021640436180075766 30-31 1.1389703252671455E-4 32-33 4.555881301068582E-4 34-35 0.002277940650534291 36-37 0.08678953878535649 38-39 0.09795144797297452 40-41 0.07870284947595975 42-43 0.0837143189071352 44-45 0.0814363782566009 46-47 0.07915843760606661 48-49 0.07574152663026518 50-51 0.0758554236627919 52-53 0.06287116195474643 54-55 0.05330381122250241 56-57 0.03177727207495336 58-59 0.027221390773884777 60-61 0.016401172683846894 62-63 0.002733528780641149 64-65 0.009111762602137164 66-67 0.16332834464330867 68-69 0.7221071862193703 70-71 0.8801962673664501 72-73 0.35524484445082266 74-75 0.054670575612822986 76-77 0.19681407220616273 78-79 0.01526220235857975 80-81 0.00398639613843501 82-83 0.0019362495529541474 84-85 2.277940650534291E-4 86-87 0.0014806614228472892 88-89 1.1389703252671455E-4 90-91 0.004783675366122011 92-93 0.005922645691389157 94-95 0.07950012870364676 96-97 0.03883888809160966 98-99 0.01754014300911404 100-101 0.01605948158626675 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 438993.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.76593277093113 #Duplication Level Percentage of deduplicated Percentage of total 1 84.18864853953004 39.37160677675212 2 9.477690788595861 8.864661004862946 3 2.824831863769347 3.9631769109066415 4 1.1998425466329534 2.24447023486158 5 0.6476325867414124 1.5143571005906558 6 0.35529301474109737 0.9969365544818162 7 0.2272429048089662 0.7439058486277051 8 0.15397160262966988 0.5760500493769332 9 0.11819743120299342 0.49748518092023525 >10 0.6640096520313963 5.85726844791555 >50 0.061974216323629865 2.055249998006358 >100 0.059023063165360885 6.022216026614871 >500 0.008853459474804131 2.7230049777726917 >1k 0.009837177194226814 10.39356140569852 >5k 0.0014755765791340221 5.342729905281831 >10k+ 0.0014755765791340221 8.833319577329526 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15014 3.4201000927121847 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12625 2.8759000712995424 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10763 2.4517475221700575 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8872 2.020988945154023 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7370 1.6788422594437726 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6985 1.5911415443982022 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4422 1.0073053556662634 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4327 0.9856649194861877 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3456 0.7872562888246509 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3215 0.7323579191467745 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3149 0.7173235108532483 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2888 0.6578692598743032 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2696 0.6141327993840449 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2516 0.5731298676744276 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2451 0.5583232534459548 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2261 0.5150423810858032 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1879 0.4280250482353933 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1686 0.38406079368008145 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1591 0.3624203575000057 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1332 0.3034216946511676 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1329 0.30273831245600724 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1263 0.287703904162481 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1253 0.28542596351194666 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1203 0.2740362602592752 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1171 0.26674685017756544 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1097 0.2498900893636117 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 971 0.22118803716687968 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 918 0.20911495171904793 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 876 0.1995476009868039 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 866 0.1972696603362696 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 674 0.15353319984601121 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 661 0.15057187700031663 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 632 0.1439658491137672 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 631 0.14373805504871376 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 627 0.14282687878850003 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 611 0.1391821737476452 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 596 0.13576526277184373 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 588 0.1339429102514163 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 551 0.12551452984443942 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 547 0.12460335358422572 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 546 0.1243755595191723 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 530 0.12073085447831741 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 510 0.11617497317724884 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 503 0.11458041472187483 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 497 0.11321365033155426 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 496 0.11298585626650084 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 485 0.11048012155091311 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 465 0.10592424024984454 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 457 0.1041018877294171 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 453 0.10319071146920338 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 448 0.10205174114393623 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.1389703252671455E-4 0.0 16-17 0.0 0.0 0.0 9.111762602137163E-4 0.0 18-19 0.0 0.0 0.0 0.0011389703252671455 0.0 20-21 0.0 0.0 0.0 0.00250573471558772 0.0 22-23 0.0 0.0 0.0 0.005922645691389157 0.0 24-25 0.0 0.0 0.0 0.00899786556961045 0.0 26-27 0.0 0.0 0.0 0.015262202358579748 0.0 28-29 0.0 0.0 0.0 0.026765802643777918 0.0 30-31 0.0 0.0 0.0 0.05307601715744898 0.0 32-33 0.0 0.0 0.0 0.09328166963937921 0.0 34-35 0.0 0.0 0.0 0.14988849480515634 0.0 36-37 0.0 0.0 0.0 0.23303332854965797 0.0 38-39 0.0 0.0 0.0 0.3505750661172274 0.0 40-41 0.0 0.0 0.0 0.489985033929926 0.0 42-43 0.0 0.0 0.0 0.6100325062130831 0.0 44-45 0.0 0.0 0.0 0.7323579191467746 0.0 46-47 0.0 0.0 0.0 0.8837270753747782 0.0 48-49 0.0 0.0 0.0 1.0606091668887658 0.0 50-51 0.0 0.0 0.0 1.2521839755986997 0.0 52-53 0.0 0.0 0.0 1.4373805504871378 0.0 54-55 0.0 0.0 0.0 1.6078844081796293 0.0 56-57 0.0 0.0 0.0 1.7913725275801664 0.0 58-59 0.0 0.0 0.0 2.0198499748287557 0.0 60-61 0.0 0.0 0.0 2.287508001266535 0.0 62-63 0.0 0.0 0.0 2.5166688307102847 0.0 64-65 0.0 0.0 0.0 2.713141211818867 0.0 66-67 0.0 0.0 0.0 2.9089302107322896 0.0 68-69 0.0 0.0 0.0 3.12419560220778 0.0 70-71 0.0 0.0 0.0 3.410760536044994 0.0 72-73 0.0 0.0 0.0 3.739125680819512 0.0 74-75 0.0 0.0 0.0 4.064529502748336 0.0 76-77 0.0 0.0 0.0 4.387541486994098 0.0 78-79 0.0 0.0 0.0 4.761579341811828 0.0 80-81 0.0 0.0 0.0 5.180834318542665 0.0 82-83 0.0 0.0 0.0 5.587446724663035 0.0 84-85 0.0 0.0 0.0 6.091668887658801 0.0 86-87 0.0 0.0 0.0 6.609217003460191 0.0 88-89 0.0 0.0 0.0 7.023688304824907 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 40 7.5674507E-6 59.335587 2 CGTGTGG 15 0.009935678 47.963474 66-67 ATAACGC 50 2.8409555E-5 47.452236 3 GTTCTAG 95 3.8380676E-10 44.99065 1 TAGACTG 45 9.6880144E-4 42.160538 5 GCGTTCA 55 1.5061232E-9 39.15261 62-63 ATGGGCG 100 1.311666E-6 33.201424 5 GTAGCGA 30 0.003771281 31.96828 64-65 CGTCTTG 30 0.0038183134 31.887466 70-71 AGTACTC 205 1.8189894E-12 30.077944 5 TGGACAT 80 4.5277624E-4 29.644129 5 TAACGCA 85 6.4257125E-4 27.922628 4 ACGCAGG 85 6.455771E-4 27.900354 7 GTATAAC 120 5.3804015E-6 27.702576 1 GGTAGCG 35 0.008011289 27.401382 64-65 CACGAAC 35 0.008422411 27.121744 88-89 ACTATAG 95 0.0012325526 24.974861 3 GGTAGCA 145 0.0 24.802979 64-65 CATGGGT 730 0.0 24.709614 4 CCGGGCG 50 0.0016608384 23.731527 60-61 >>END_MODULE