FastQCFastQC Report
Fri 27 May 2016
SRR939383_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939383_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934442
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85730.4431768954561574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69860.36113773377542463No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45110.23319386158902672No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28000.1447445826755209No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21120.10917877093239291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90050.031.2723621
GTATCAA130950.030.2676751
GTACATG94900.024.7636551
TACATGG94700.024.652392
TATCAAC160350.024.51932
ATCAACG161800.024.1430663
TCAACGC164550.023.6031654
CAACGCA165050.023.5374075
AACGCAG168150.022.8962616
ACATGGG98850.022.692513
GAGTACT90300.022.33963812-13
GTACTTT97600.020.96848314-15
ACGCAGA184700.020.540737
CGCAGAG186050.020.524758
AGTACTT95150.020.21894612-13
AGAGTAC145600.019.61791410-11
CATGGGG34800.019.4038164
GCAGAGT185650.019.2726489
ACTTTTT118400.018.69512216-17
CATGGGA66900.018.0393854
Produced by FastQC (version 0.11.5)