FastQCFastQC Report
Fri 27 May 2016
SRR939383_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939383_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934442
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123800.6399778334010531No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112520.5816664443803432No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79210.4094720854902861No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44390.22947185803451328No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32160.16624949210159828No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29540.15270553472267454No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23870.12339475673088156No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19640.10152798584811538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106450.030.7641941
GTATCAA150050.026.6259271
ATCAACG182150.021.8454593
TATCAAC183100.021.787242
GTACATG104550.021.7585741
TCAACGC184000.021.6258184
CAACGCA185550.021.3685745
AACGCAG187500.021.1463416
TACATGG105400.021.0819442
GAGTACT104200.020.98154812-13
AGTACTT107850.020.0957612-13
GTACTTT110300.019.79971514-15
ACATGGG106400.019.6784883
AGAGTAC171200.018.81600810-11
ACGCAGA211050.018.7193937
CGCAGAG212300.018.5868638
ACTTTTT126350.017.71554416-17
TACTTTT121800.017.65802214-15
GCAGAGT217800.017.5085059
TGCACCG3251.0375516E-817.490775
Produced by FastQC (version 0.11.5)