##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939383_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1934442 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.981074128870237 31.0 31.0 31.0 30.0 34.0 2 31.231222233595012 31.0 31.0 34.0 30.0 34.0 3 31.212056500013958 31.0 31.0 34.0 28.0 34.0 4 35.14841179006659 35.0 35.0 37.0 33.0 37.0 5 34.77556680427741 35.0 35.0 37.0 32.0 37.0 6 34.736001389547994 35.0 35.0 37.0 32.0 37.0 7 34.61533661903536 35.0 35.0 37.0 30.0 37.0 8 34.6377291229202 35.0 35.0 37.0 30.0 37.0 9 35.97784632467658 37.0 35.0 39.0 30.0 39.0 10-11 35.83984167010435 37.0 35.0 39.0 30.0 39.0 12-13 35.80153863491384 37.0 35.0 39.0 30.0 39.0 14-15 36.6297810944965 38.0 35.5 40.0 30.0 41.0 16-17 36.64981994807805 38.0 35.5 40.0 30.5 41.0 18-19 36.64397433471771 38.0 35.0 40.0 30.0 41.0 20-21 36.554145329764346 38.0 35.0 40.0 30.0 41.0 22-23 36.40557044356977 38.0 35.0 40.0 30.0 41.0 24-25 36.1861859905854 38.0 34.0 40.0 29.5 41.0 26-27 35.80465581289074 38.0 34.0 40.0 28.0 41.0 28-29 35.5265686435675 38.0 34.0 40.0 27.0 41.0 30-31 35.29595898972417 38.0 34.0 40.0 27.0 41.0 32-33 35.05898884536212 37.5 33.0 40.0 27.0 41.0 34-35 34.98624538755879 38.0 33.0 40.0 26.5 41.0 36-37 34.95349330711389 38.0 33.0 40.0 25.5 41.0 38-39 34.915183551639174 38.0 33.0 40.0 26.0 41.0 40-41 34.69352867648655 38.0 33.0 40.0 25.0 41.0 42-43 34.37577890678553 37.5 33.0 40.0 24.0 41.0 44-45 34.16318969501283 37.0 33.0 40.0 24.0 41.0 46-47 33.80600271292704 37.0 33.0 40.0 23.5 41.0 48-49 33.457708217666905 37.0 32.0 40.0 22.0 41.0 50-51 33.18439813651689 37.0 31.5 40.0 20.5 41.0 52-53 32.828393149032124 37.0 31.0 40.0 19.0 41.0 54-55 32.62044015793702 36.0 31.0 39.0 18.5 41.0 56-57 32.23622186656411 36.0 31.0 39.0 15.5 40.0 58-59 31.809905905682363 35.5 30.0 39.0 13.5 40.0 60-61 31.527336565273085 35.0 30.0 39.0 12.5 40.0 62-63 31.15828440449494 35.0 29.0 38.5 8.0 40.0 64-65 30.74157224667372 34.5 28.5 38.0 8.0 40.0 66-67 29.064456055027755 33.5 25.0 37.5 4.5 39.5 68-69 25.422301624964717 30.0 16.0 35.0 2.0 39.0 70-71 24.801631426530236 29.5 14.0 35.0 2.0 38.0 72-73 26.631882217197518 31.0 21.5 35.0 2.0 38.0 74-75 27.23997152667281 32.0 23.5 35.0 2.0 38.0 76-77 27.28462884904277 31.5 25.0 34.5 2.0 36.5 78-79 28.11276791963781 33.0 26.0 35.0 2.0 37.0 80-81 27.96036609006628 33.0 26.0 35.0 2.0 37.0 82-83 27.541774061977563 33.0 26.0 35.0 2.0 36.0 84-85 27.193864949168805 32.5 26.0 35.0 2.0 36.0 86-87 26.743256194809668 32.0 25.0 35.0 2.0 35.5 88-89 26.241726813210217 32.0 24.5 34.0 2.0 35.0 90-91 25.776645151418343 32.0 23.5 34.0 2.0 35.0 92-93 25.222281412417637 31.5 20.5 34.0 2.0 35.0 94-95 23.850990363112462 31.0 10.0 34.0 2.0 35.0 96-97 23.044180440664544 30.0 4.0 34.0 2.0 35.0 98-99 22.695002228032685 30.0 2.0 34.0 2.0 35.0 100-101 21.452402553294437 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4046350502147398 1101 2 0.26410152728559666 1101 3 0.3472504832592236 1101 4 0.30082660740493594 1101 5 0.4274238769765262 1101 6 0.5131207033874432 1101 7 0.5452194031543485 1101 8 0.4628234192822376 1101 9 0.652180833977134 1101 10-11 0.8188052667225705 1101 12-13 0.69472748647609 1101 14-15 0.75941544663889 1101 16-17 0.789740657132306 1101 18-19 0.8735493681566666 1101 20-21 0.8475224628673885 1101 22-23 0.9126842463768838 1101 24-25 0.9025863496215223 1101 26-27 0.894492981616402 1101 28-29 0.7480177929051379 1101 30-31 0.8027549774784575 1101 32-33 0.9104187922162268 1101 34-35 0.7727631716027616 1101 36-37 0.8456874081248955 1101 38-39 0.8583688743090505 1101 40-41 0.9386673040812852 1101 42-43 0.8241188004692575 1101 44-45 0.6361090354490315 1101 46-47 0.5806191433800336 1101 48-49 0.7481494686431276 1101 50-51 0.5674049702206716 1101 52-53 0.8688840548795582 1101 54-55 0.7700711403293283 1101 56-57 0.6918337804926225 1101 58-59 0.6576627328267755 1101 60-61 0.44478190972066045 1101 62-63 0.44701935307382357 1101 64-65 0.18184277191370057 1101 66-67 -2.7292289282576583 1101 68-69 -7.5616539876412645 1101 70-71 -11.151429918260703 1101 72-73 -11.100872959711822 1101 74-75 -9.534934837917401 1101 76-77 -4.54421370192744 1101 78-79 -1.5646894754086418 1101 80-81 -0.8026275481367762 1101 82-83 -0.7395823306708493 1101 84-85 -0.6496021623677777 1101 86-87 -0.4678751876957179 1101 88-89 -0.38297377222712115 1101 90-91 -0.39229983441387617 1101 92-93 -1.1682289458476056 1101 94-95 -4.718102034094098 1101 96-97 -1.5886468133677667 1101 98-99 -0.9424104220038174 1101 100-101 -0.8265749970752054 1102 1 0.4491396949518709 1102 2 0.36036086160996916 1102 3 0.40530886376019737 1102 4 0.21595353154123131 1102 5 0.42064734255265535 1102 6 0.34779020311367503 1102 7 0.41106256182902 1102 8 0.5054933778467401 1102 9 0.6472123090821569 1102 10-11 0.650192205437655 1102 12-13 0.5603945793021481 1102 14-15 0.5521979472941396 1102 16-17 0.6450805542538234 1102 18-19 0.5978295822900961 1102 20-21 0.8523125877074094 1102 22-23 0.7864477005754225 1102 24-25 0.6938469477518865 1102 26-27 0.6529956616187249 1102 28-29 0.5673322164995582 1102 30-31 0.327675297678212 1102 32-33 0.43445878317284325 1102 34-35 0.615946855633922 1102 36-37 0.8793343254052886 1102 38-39 0.7042575700145761 1102 40-41 0.6808965644913556 1102 42-43 0.8206911852078136 1102 44-45 0.6517562208296965 1102 46-47 0.6519474868471491 1102 48-49 0.5428553553114099 1102 50-51 0.6527035385518971 1102 52-53 0.6393818877357091 1102 54-55 0.4672352130258517 1102 56-57 0.6872410628162058 1102 58-59 0.5628586094179546 1102 60-61 0.5150835358621713 1102 62-63 0.3787918359824296 1102 64-65 0.3628114542008767 1102 66-67 -2.711375584440404 1102 68-69 -7.587120178663373 1102 70-71 -10.851032569652325 1102 72-73 -9.1360661219545 1102 74-75 -7.519431706412885 1102 76-77 -4.064456606106937 1102 78-79 -1.2438021302082056 1102 80-81 -0.519884802349857 1102 82-83 -0.17881727120527557 1102 84-85 -0.2454623474103208 1102 86-87 -0.21650317456280632 1102 88-89 -0.22116192126152612 1102 90-91 -0.41263110497573763 1102 92-93 -0.8345851630728482 1102 94-95 -4.3728699172824115 1102 96-97 -1.1626791681351172 1102 98-99 -0.5343284660189447 1102 100-101 -0.5078654663773925 1103 1 0.44240287618115914 1103 2 0.2862983362722318 1103 3 0.398925695089801 1103 4 0.24959751629805282 1103 5 0.5243235353454381 1103 6 0.5481795632485102 1103 7 0.5911847522514861 1103 8 0.46830232571181796 1103 9 0.7415514923056818 1103 10-11 0.753254362461746 1103 12-13 0.5788632010500763 1103 14-15 0.8406925450732174 1103 16-17 0.7699346735874499 1103 18-19 0.7468619395491416 1103 20-21 0.7418886863085064 1103 22-23 0.7074349363944847 1103 24-25 0.8828538422037226 1103 26-27 0.8238175013237949 1103 28-29 0.9267972732819629 1103 30-31 0.9602516041565607 1103 32-33 0.9004492880989332 1103 34-35 0.9706868020516239 1103 36-37 0.8480013577979122 1103 38-39 0.9664002755931023 1103 40-41 0.9143277338310938 1103 42-43 0.9535896583019436 1103 44-45 0.804793773294989 1103 46-47 0.7335588245243443 1103 48-49 0.46718048741265505 1103 50-51 0.518966655951651 1103 52-53 0.6761522256354482 1103 54-55 0.4967163695134289 1103 56-57 0.6226705924270775 1103 58-59 0.5701256876988303 1103 60-61 0.5984743373368104 1103 62-63 0.6277925588087321 1103 64-65 0.42132976378789877 1103 66-67 -2.583623151586483 1103 68-69 -7.520675254922413 1103 70-71 -11.422362608864358 1103 72-73 -9.299866928966779 1103 74-75 -7.564846201581677 1103 76-77 -3.8781040281702204 1103 78-79 -1.1112816745688647 1103 80-81 -0.23800529438927498 1103 82-83 -0.09824661364035592 1103 84-85 -0.25309515164859775 1103 86-87 -0.27926679483575256 1103 88-89 -0.13968104596890285 1103 90-91 -0.0014026711626442534 1103 92-93 0.23426264058751656 1103 94-95 0.999697875716695 1103 96-97 1.0906775966737676 1103 98-99 0.9953929137684838 1103 100-101 1.0056600326574028 1104 1 0.45739671444971464 1104 2 0.22312658501012805 1104 3 0.3028420705913142 1104 4 0.1887968062985408 1104 5 0.41709978047031626 1104 6 0.45907777564619323 1104 7 0.4325954386543671 1104 8 0.2838669993851184 1104 9 0.6593606466780599 1104 10-11 0.7115904674072411 1104 12-13 0.7015442369211584 1104 14-15 0.7852404630381642 1104 16-17 0.7068535102805598 1104 18-19 0.7195879630617554 1104 20-21 0.7403478158238954 1104 22-23 0.7881046045712381 1104 24-25 0.8175337997718017 1104 26-27 0.9785601089965539 1104 28-29 1.050312856160751 1104 30-31 0.9489180444262786 1104 32-33 0.885363549006243 1104 34-35 0.8248576990736822 1104 36-37 0.920038475227912 1104 38-39 0.832176058588125 1104 40-41 0.947689045859768 1104 42-43 1.1480019131455563 1104 44-45 0.8868647102282665 1104 46-47 0.784898563490998 1104 48-49 0.7681049221788285 1104 50-51 0.7665177178000278 1104 52-53 0.5067711295474453 1104 54-55 0.5853669678315612 1104 56-57 0.6728239355164831 1104 58-59 0.760538943823807 1104 60-61 0.6756863464113536 1104 62-63 0.7113207802389532 1104 64-65 0.4475232491572889 1104 66-67 -2.865443593130557 1104 68-69 -7.494388555859974 1104 70-71 -8.8320744253848 1104 72-73 -8.097056689289431 1104 74-75 -6.785385423102927 1104 76-77 -3.5289741101632615 1104 78-79 -0.7396461199780227 1104 80-81 -0.23378354557786096 1104 82-83 -0.044869302115813525 1104 84-85 -0.039923750484870624 1104 86-87 0.2286436780071135 1104 88-89 0.20728526406421466 1104 90-91 0.14712744079881546 1104 92-93 0.09441890699837785 1104 94-95 0.9953409014069123 1104 96-97 1.2647058111020222 1104 98-99 0.7563619335630491 1104 100-101 0.6889623531094458 1105 1 0.4881260124872071 1105 2 0.28178545060791294 1105 3 0.3840812694027065 1105 4 0.35849411900291983 1105 5 0.3095428923534769 1105 6 0.4732912262956219 1105 7 0.5676999812365651 1105 8 0.49652530169451126 1105 9 0.7795186615499432 1105 10-11 0.7952516057667296 1105 12-13 0.7402523768840581 1105 14-15 0.8579985841505149 1105 16-17 0.9379436872261948 1105 18-19 0.9293674338737006 1105 20-21 0.9276513444869892 1105 22-23 0.9261684165897819 1105 24-25 1.1041307223633154 1105 26-27 1.1028115459612877 1105 28-29 1.1432914262460443 1105 30-31 1.0484482957758985 1105 32-33 1.015892933141295 1105 34-35 1.055496554564364 1105 36-37 1.1430132217258588 1105 38-39 0.8725937832773596 1105 40-41 1.160880890121227 1105 42-43 1.2105336092585972 1105 44-45 1.0167024617806533 1105 46-47 1.089228486493397 1105 48-49 0.9377895341560176 1105 50-51 0.9224985793638822 1105 52-53 1.1550573558093475 1105 54-55 0.9403138565099098 1105 56-57 1.0604677322584948 1105 58-59 1.291522702044741 1105 60-61 1.1088880972400261 1105 62-63 1.1920952215629264 1105 64-65 1.1630466795479464 1105 66-67 1.9236453458284117 1105 68-69 -4.760368521688335 1105 70-71 -4.934122651605698 1105 72-73 -1.308419963064523 1105 74-75 1.406145887977548 1105 76-77 1.3827239726062643 1105 78-79 1.255646478038166 1105 80-81 1.3061279325817878 1105 82-83 1.521604298642842 1105 84-85 1.392929955854708 1105 86-87 1.4845255630267609 1105 88-89 1.5912317008640606 1105 90-91 1.7266696442011842 1105 92-93 1.7022927562456474 1105 94-95 2.417913034852784 1105 96-97 2.518168653230642 1105 98-99 2.268892092219641 1105 100-101 1.8442630573090675 1106 1 0.22256055316738355 1106 2 0.20787728182303766 1106 3 0.20697535431825997 1106 4 0.13438768322171057 1106 5 0.35059199632204496 1106 6 0.29914178742386355 1106 7 0.28513659701092564 1106 8 0.3020218725469661 1106 9 0.5345010525921126 1106 10-11 0.4498488702294665 1106 12-13 0.40764935244691713 1106 14-15 0.43367193474076515 1106 16-17 0.44732721874593295 1106 18-19 0.4264532683941127 1106 20-21 0.48173666006901783 1106 22-23 0.5857893387967223 1106 24-25 0.528895535131177 1106 26-27 0.5709327713768246 1106 28-29 0.7498045756525968 1106 30-31 0.6692780784877499 1106 32-33 0.6362719142241176 1106 34-35 0.7462511428611478 1106 36-37 0.8238240184578345 1106 38-39 0.6525390672571092 1106 40-41 0.7824301750918181 1106 42-43 0.7708993762049303 1106 44-45 0.9385573950912374 1106 46-47 0.8139476036695044 1106 48-49 0.8494828580498393 1106 50-51 0.5560627937590752 1106 52-53 0.7637031859602104 1106 54-55 0.9229848089058308 1106 56-57 0.7184155671908101 1106 58-59 0.798219007471527 1106 60-61 0.8390620020459352 1106 62-63 0.9704177515557468 1106 64-65 0.8875845749087752 1106 66-67 1.3406561947399709 1106 68-69 -5.247611121321317 1106 70-71 -6.218220102125766 1106 72-73 -2.33372090227488 1106 74-75 0.5465069644768903 1106 76-77 0.6804238292772844 1106 78-79 0.47686479416769956 1106 80-81 0.6025841833936596 1106 82-83 0.7224646681682998 1106 84-85 0.8292814255097802 1106 86-87 0.8220078392255132 1106 88-89 0.7937268032593288 1106 90-91 0.5934588617999026 1106 92-93 0.6642929923065672 1106 94-95 1.7660703639619335 1106 96-97 1.7213662236934013 1106 98-99 1.5022701833483865 1106 100-101 1.3221468524741269 1107 1 -0.08166931789006426 1107 2 0.0053338065312402705 1107 3 -0.04136500566409751 1107 4 -0.034286821349155616 1107 5 0.07669866555607285 1107 6 0.06307212844210142 1107 7 0.03233396039428982 1107 8 -0.03448550599424749 1107 9 -2.3596863112373967E-4 1107 10-11 0.06321812418070039 1107 12-13 0.18780823009367253 1107 14-15 0.058486379710124936 1107 16-17 -0.10284887661647701 1107 18-19 0.1557654777472095 1107 20-21 0.12895921191807247 1107 22-23 0.15306515585456992 1107 24-25 0.31739784015567096 1107 26-27 0.27810297599672396 1107 28-29 0.2826978011867638 1107 30-31 0.12462553378102825 1107 32-33 0.09377897259194867 1107 34-35 0.0659594940129935 1107 36-37 0.158365241341059 1107 38-39 0.3260040821574819 1107 40-41 0.3137647383685973 1107 42-43 0.27443527704521387 1107 44-45 0.2793426706978295 1107 46-47 0.06247816455506694 1107 48-49 0.2776366218783082 1107 50-51 0.18582738795610254 1107 52-53 0.34011301237249825 1107 54-55 0.3552513454134285 1107 56-57 0.34546495673435373 1107 58-59 0.24534505950977703 1107 60-61 0.08691538502479546 1107 62-63 0.27626181144384176 1107 64-65 0.3630669060397054 1107 66-67 1.0854913278358218 1107 68-69 -5.792260867309636 1107 70-71 -5.934120628956521 1107 72-73 -2.4538950721190247 1107 74-75 0.2021319406877815 1107 76-77 0.15974555427297332 1107 78-79 0.4003834325128288 1107 80-81 0.29975512687522254 1107 82-83 0.40860754162368806 1107 84-85 0.4140220290925889 1107 86-87 0.6701245256324739 1107 88-89 0.48913544973193623 1107 90-91 0.3756939407125657 1107 92-93 0.5007086848326487 1107 94-95 1.195276893707831 1107 96-97 1.4972598544819924 1107 98-99 1.1789142065172982 1107 100-101 0.905550788143902 1108 1 -0.02583564322190668 1108 2 -0.11027562241185151 1108 3 -0.04927797203623996 1108 4 -0.01475691816821012 1108 5 -0.04506469263844082 1108 6 0.19080487002838709 1108 7 0.09983273383949154 1108 8 0.07848446704404921 1108 9 0.19019645382585537 1108 10-11 0.1558981801746171 1108 12-13 0.3347560216198602 1108 14-15 0.21111818371342395 1108 16-17 0.11549790755939426 1108 18-19 0.20072107421292884 1108 20-21 0.2985802627491978 1108 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0.7794258315477371 2311 60-61 0.8711853820512658 2311 62-63 1.0106561563199001 2311 64-65 0.9728552443739851 2311 66-67 1.931112120958847 2311 68-69 4.532788466348084 2311 70-71 4.897423176628319 2311 72-73 2.9133568404049655 2311 74-75 2.0203475490461216 2311 76-77 1.39892864296543 2311 78-79 0.9689666779917019 2311 80-81 0.6243151787769321 2311 82-83 0.6189703129588047 2311 84-85 0.6085872171011282 2311 86-87 0.8665022187095417 2311 88-89 1.1054696210616477 2311 90-91 0.9270550090227871 2311 92-93 1.0639003697460119 2311 94-95 1.4503846167905756 2311 96-97 1.5002279835874965 2311 98-99 1.2621718498552248 2311 100-101 1.076206047246977 2312 1 -0.19857857889343222 2312 2 -0.16550951459100105 2312 3 -0.23853577086784838 2312 4 -0.07037137710385366 2312 5 -0.007637856519963293 2312 6 -0.018316407833651738 2312 7 0.0317512300031666 2312 8 0.041524901255549196 2312 9 -1.6778871556686852 2312 10-11 -1.0713017217264778 2312 12-13 -2.172410183556714 2312 14-15 -2.229189502496432 2312 16-17 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1.2272040433514029 2312 84-85 1.05408159358824 2312 86-87 1.2337215155937606 2312 88-89 1.3597026826445493 2312 90-91 1.3733598692540667 2312 92-93 1.3782598627355114 2312 94-95 2.0739820352798155 2312 96-97 2.6724430995657933 2312 98-99 2.470942927250846 2312 100-101 2.0897387927175473 2313 1 -0.3018610515394933 2313 2 -0.22600345491927598 2313 3 -0.0626177924065594 2313 4 0.02940001369011469 2313 5 -0.015148905039005456 2313 6 0.07003285592248432 2313 7 -3.348376157824159 2313 8 -1.1264931430385303 2313 9 -0.7586642603039238 2313 10-11 -0.07891554716682236 2313 12-13 -1.4694257639824357 2313 14-15 -0.5096593874696183 2313 16-17 -0.13474507280314896 2313 18-19 0.03364400605877904 2313 20-21 -0.09011162397489159 2313 22-23 -1.499363383849115 2313 24-25 -2.7834167682871893 2313 26-27 -2.4608772420974176 2313 28-29 -0.5936541834930082 2313 30-31 -0.16012806483000475 2313 32-33 -2.5086282249185174 2313 34-35 -2.813852521641799 2313 36-37 -1.1978966735151246 2313 38-39 -0.6603510461696658 2313 40-41 -0.7280496653045603 2313 42-43 -0.8271225588563951 2313 44-45 -0.12215200384814295 2313 46-47 -0.15599710878363737 2313 48-49 -0.43477357507552483 2313 50-51 -0.25875227178011784 2313 52-53 0.395805898211961 2313 54-55 0.31615187060442906 2313 56-57 0.5035640306562144 2313 58-59 0.11267232473844402 2313 60-61 0.5434595071252133 2313 62-63 0.32068428881102307 2313 64-65 0.16428110378954486 2313 66-67 1.5665381624136394 2313 68-69 4.542117512471808 2313 70-71 4.903553533434497 2313 72-73 2.8056555593503276 2313 74-75 1.7793178571475146 2313 76-77 1.1596408621629521 2313 78-79 0.6040894755259991 2313 80-81 0.6852950699434786 2313 82-83 0.6626420813677534 2313 84-85 0.8582665587135345 2313 86-87 0.8712175920153449 2313 88-89 0.8491388817873364 2313 90-91 1.1093231806709198 2313 92-93 1.103105211246497 2313 94-95 1.6046415020064835 2313 96-97 1.8211668602011208 2313 98-99 1.5233661015021696 2313 100-101 1.2246348908056213 2314 1 -0.33284078658155636 2314 2 -0.32234717953075176 2314 3 -0.2303634714431695 2314 4 -0.29737775644380804 2314 5 -1.6049706448246255 2314 6 -2.93904067580171 2314 7 -0.6396255517754668 2314 8 -0.3005564702130954 2314 9 -0.02746774000620178 2314 10-11 0.22349967972470353 2314 12-13 -0.0013712867567576836 2314 14-15 -2.6910049220373224 2314 16-17 -3.2626815425203617 2314 18-19 -0.6595409763821891 2314 20-21 -0.24165655675261632 2314 22-23 -0.05261991279531486 2314 24-25 -2.467253449630064 2314 26-27 -1.2317599192601918 2314 28-29 -0.33509006026528 2314 30-31 -0.13651284514553907 2314 32-33 -0.31802120163314385 2314 34-35 -0.1488307857873039 2314 36-37 0.12755925527905987 2314 38-39 -1.6099298423276451 2314 40-41 -4.114831579501171 2314 42-43 -5.334437525638371 2314 44-45 -2.5449588037144792 2314 46-47 -3.5518349465329315 2314 48-49 -1.2880199076619476 2314 50-51 -1.4141063024953269 2314 52-53 -2.831756786878593 2314 54-55 -1.8619416803822801 2314 56-57 -3.9090619701431173 2314 58-59 -5.372851710825149 2314 60-61 -3.0247934510476 2314 62-63 -1.8543413454107451 2314 64-65 -1.0777163917551782 2314 66-67 -0.8331468560626583 2314 68-69 1.018375964394128 2314 70-71 1.009226313003122 2314 72-73 -1.1403029148483768 2314 74-75 -0.7357711173525736 2314 76-77 -0.5714436619741647 2314 78-79 -0.2628238693386358 2314 80-81 0.010251213311669005 2314 82-83 -0.1343711223039179 2314 84-85 -0.20700979949073073 2314 86-87 -0.5418121682514432 2314 88-89 -0.2452532022339433 2314 90-91 0.37100026482323756 2314 92-93 0.42966287923664126 2314 94-95 0.16876022530758306 2314 96-97 0.5802680326596494 2314 98-99 0.06551740237750181 2314 100-101 0.22383597121026355 2315 1 -0.37993619720957383 2315 2 -0.36723043234878716 2315 3 -0.48126222345605285 2315 4 -0.17686346497843175 2315 5 -1.2029929878197905 2315 6 -0.4884146693604876 2315 7 -0.3303773633696707 2315 8 -0.12742543156575437 2315 9 -0.049699020199440724 2315 10-11 -2.0003615320305315 2315 12-13 -1.2661453407020034 2315 14-15 -0.11720208387307451 2315 16-17 -1.8525174926008816 2315 18-19 -3.220653093934523 2315 20-21 -2.2361976195545523 2315 22-23 -1.6537964425859712 2315 24-25 -1.2407538522065522 2315 26-27 -0.360583892690137 2315 28-29 -0.2880872422298566 2315 30-31 -2.8900811825046375 2315 32-33 -2.5017606340002914 2315 34-35 -1.5597396022124244 2315 36-37 -5.00123603457601 2315 38-39 -1.9719296410393952 2315 40-41 -1.4951737695172724 2315 42-43 -1.541946080388776 2315 44-45 -1.4038169961460483 2315 46-47 -1.284436597096537 2315 48-49 -4.482705394779497 2315 50-51 -3.8174013910621554 2315 52-53 -2.3979252651394596 2315 54-55 -4.08454131806344 2315 56-57 -6.483457602188203 2315 58-59 -6.900467451565891 2315 60-61 -6.005907581482383 2315 62-63 -6.917573028180474 2315 64-65 -6.3535643184862565 2315 66-67 -4.853627934967651 2315 68-69 -2.306622022723424 2315 70-71 0.2840310634056955 2315 72-73 -1.0419333496309875 2315 74-75 -2.863790441024072 2315 76-77 -5.147349962296225 2315 78-79 -4.232707122156672 2315 80-81 -3.848366439667398 2315 82-83 -7.0252361585358045 2315 84-85 -4.979183712534205 2315 86-87 -5.306262148122624 2315 88-89 -3.931245553280643 2315 90-91 -3.5342050491864256 2315 92-93 -4.314412704499265 2315 94-95 -2.5853752416811453 2315 96-97 -1.5633284850537201 2315 98-99 -3.202948277976766 2315 100-101 -2.68788001107637 2316 1 -0.46768756188645355 2316 2 -0.3805062438179405 2316 3 -0.911469419558653 2316 4 -0.3963892030778595 2316 5 -0.2751359707393206 2316 6 -2.2442777908020517 2316 7 -0.6424323026195324 2316 8 -0.4901160461105718 2316 9 -0.042046264432578084 2316 10-11 -0.3257433480779568 2316 12-13 -0.9417108745401279 2316 14-15 -1.0931696838026355 2316 16-17 -1.3989828327429237 2316 18-19 -0.3052002559935687 2316 20-21 -4.243172439065027 2316 22-23 -4.128238539199224 2316 24-25 -3.000101155135475 2316 26-27 -2.2519503304432718 2316 28-29 -1.8409920963708473 2316 30-31 -0.4451020195064572 2316 32-33 -2.1802885438435737 2316 34-35 -2.4220287376235845 2316 36-37 -4.629218073667396 2316 38-39 -1.9029849911597267 2316 40-41 -1.6647394207469475 2316 42-43 -3.081356774759854 2316 44-45 -0.8626518849175397 2316 46-47 -0.6575386690575584 2316 48-49 -0.36002593243284053 2316 50-51 -0.005044168546454841 2316 52-53 -2.7506194014310594 2316 54-55 -0.8668973666635473 2316 56-57 -0.12865980914952502 2316 58-59 -0.08692163950977516 2316 60-61 -1.5227855529562326 2316 62-63 -0.9874450713805665 2316 64-65 0.2687224690937171 2316 66-67 1.2362499778104201 2316 68-69 3.742143018070145 2316 70-71 4.028069333054109 2316 72-73 2.1876229102739266 2316 74-75 1.129298130907607 2316 76-77 -0.24416219449673093 2316 78-79 0.2370598555976251 2316 80-81 -0.2211022499626374 2316 82-83 0.06807076169663162 2316 84-85 0.12219505133267106 2316 86-87 -0.26686847552768356 2316 88-89 0.5077161183751642 2316 90-91 0.4677195854323486 2316 92-93 0.35538949072289583 2316 94-95 1.1373476555425448 2316 96-97 1.0513806773575034 2316 98-99 0.012946539185879402 2316 100-101 -0.2928268913956771 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 35.0 4 69.0 5 168.0 6 398.0 7 952.0 8 1995.0 9 3279.0 10 5326.0 11 7855.0 12 10360.0 13 12933.0 14 15904.0 15 20032.0 16 23959.0 17 27259.0 18 24577.0 19 20547.0 20 19669.0 21 20000.0 22 21033.0 23 24064.0 24 29245.0 25 36290.0 26 45608.0 27 56871.0 28 69494.0 29 81527.0 30 96313.0 31 112630.0 32 129948.0 33 151331.0 34 176107.0 35 201814.0 36 215336.0 37 185464.0 38 80835.0 39 5212.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.5329360885383 20.895889736647796 15.588152563511251 21.983021611302654 2 15.75861956641447 22.001241947634483 39.01664840627923 23.22349007967182 3 19.4373364515452 25.74411639118671 30.745248500601207 24.073298656666882 4 12.909562550854458 18.10527273497991 37.17025374759233 31.814910966573308 5 12.793476558320007 38.726835015216295 34.178487923372096 14.301200503091593 6 30.443507740216557 37.53702618119333 17.29320393167642 14.726262146913683 7 27.03952871163881 34.15129530893146 20.74934270451117 18.059833274918553 8 25.955960426831098 35.40137155830984 19.550960948945484 19.09170706591358 9 25.91693108400252 17.067143910233547 20.926086178856746 36.08983882690719 10-11 24.555970145395932 27.634015390484695 26.927429201805996 20.88258526231337 12-13 25.83473683884035 25.18286927186238 27.78752218986147 21.194871699435804 14-15 22.606002144287604 26.36165881427306 27.039709642367153 23.99262939907219 16-17 20.940439244193335 29.600273774817627 27.73619087522387 21.723096105765165 18-19 20.683552489927877 27.67367125140901 30.958706818853265 20.684069439809846 20-21 21.951785000254596 26.469036638261368 30.996497951990744 20.582680409493292 22-23 21.32377191975774 26.759370402420956 30.866265310616704 21.050592367204597 24-25 21.244059010298578 26.88485878615125 31.110315015906398 20.760767187643776 26-27 21.12616716745824 26.950676876352947 31.094000193854804 20.82915576233401 28-29 20.71793824454004 27.244891628060884 31.181491975512905 20.855678151886167 30-31 21.012135304980056 26.919346800692324 31.072770472446642 20.995747421880978 32-33 20.87882943372913 26.997574029579518 30.990279316178782 21.133317220512566 34-35 21.427535589384927 26.99026159758604 30.813604966680874 20.768597846348158 36-37 21.307445033677354 26.80358569612969 30.91591736111974 20.97305190907322 38-39 21.02033344416227 27.21470497294178 30.944508339985227 20.820453242910723 40-41 21.25303418471651 27.03092410009575 30.833889708355976 20.882152006831767 42-43 21.028147651360964 27.21542503507266 30.47626366358814 21.28016364997824 44-45 21.23114658028494 27.322274299064812 30.199210093621886 21.247369027028356 46-47 21.444088137568375 27.520971767351675 29.72156489152017 21.31337520355978 48-49 21.43796738118537 27.58666102958794 29.639244210566623 21.33612737866007 50-51 21.30038334081938 27.80030779038791 29.551887622355476 21.34742124643723 52-53 21.595755193095567 27.97393350830331 29.080869552171006 21.349441746430113 54-55 21.29193403169609 27.77087335348879 29.230079241524347 21.707113373290767 56-57 21.542735910162214 28.010363511544533 28.938166708253444 21.508733870039805 58-59 21.73940805579755 27.70688188776895 29.05839044327309 21.495319613160405 60-61 21.505610937113868 27.809216764257922 29.439661100018714 21.245511198609492 62-63 21.394614758700122 27.77280063358213 29.397543835343086 21.435040772374663 64-65 21.278635030790802 27.963252658265542 29.20791234651127 21.550199964432387 66-67 21.014893574496813 27.79685724008274 29.439773285632224 21.74847589978822 68-69 21.145966911210003 27.495789568866968 29.887898166850075 21.470345353072958 70-71 20.852444359593488 27.41542246126545 30.14336334240439 21.588769836736674 72-73 21.45283221334561 27.590992198706193 29.152365504274407 21.80381008367379 74-75 20.988746303235033 28.084567745442545 29.054495330913042 21.87219062040938 76-77 21.18014265653856 28.0069704822155 28.81843553467249 21.99445132657345 78-79 21.21511503469497 27.94348922548165 28.867525278061258 21.973870461762118 80-81 21.210440338706178 28.06115011217833 28.685497461771487 22.04291208734401 82-83 21.220046917922584 28.09032268736928 28.68212125253691 22.00750914217123 84-85 21.129995109700886 28.064966538154156 28.590673692982264 22.214364659162694 86-87 21.1245114865284 28.098752093629166 28.588376997994253 22.188359421848183 88-89 21.156610337368377 28.02626202002297 28.386962867844428 22.43016477476423 90-91 21.06546717904405 28.05421267728212 28.52239850308742 22.357921640586408 92-93 21.15237133947046 28.096014143902316 28.585726083377168 22.16588843325006 94-95 20.679400361167662 27.94396849743424 28.820153684068185 22.556477457329912 96-97 20.51367410743431 27.853526273084224 28.981558530112466 22.651241089369005 98-99 20.819788628936838 27.852427499694315 28.3751809227831 22.952602948585746 100-101 20.61647091545497 27.754451803119522 28.6266534077823 23.00242387364321 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3842.0 1 2559.0 2 1315.5 3 1408.0 4 1319.0 5 1234.5 6 1755.5 7 3179.0 8 4632.0 9 5306.0 10 4912.0 11 4007.5 12 3587.5 13 3474.5 14 3523.5 15 3641.5 16 3796.0 17 3986.5 18 4213.5 19 4604.0 20 5186.5 21 5945.5 22 6909.5 23 8222.5 24 9585.5 25 11252.0 26 14058.0 27 17377.0 28 20136.5 29 23012.5 30 26401.0 31 29811.5 32 34202.5 33 38654.0 34 42780.5 35 46844.0 36 51417.0 37 55901.5 38 58171.0 39 58992.5 40 59965.5 41 61983.0 42 63487.0 43 64705.5 44 66710.0 45 68506.0 46 69586.0 47 71701.5 48 76319.5 49 75806.0 50 69213.0 51 63944.0 52 60602.0 53 57970.0 54 54931.0 55 50902.5 56 46050.5 57 41063.0 58 36708.5 59 32171.5 60 26951.5 61 21666.5 62 16973.0 63 12839.5 64 9395.0 65 6960.0 66 5077.0 67 3703.0 68 2599.0 69 1756.0 70 1185.0 71 861.5 72 703.0 73 628.5 74 495.5 75 362.0 76 273.0 77 169.5 78 101.0 79 65.5 80 44.0 81 28.5 82 19.5 83 10.0 84 6.0 85 3.0 86 1.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02346930019095946 2 0.01979899113025875 3 0.0 4 0.0 5 1.5508348143805813E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.843197159697732E-4 18-19 9.563481355346917E-4 20-21 1.292362345317151E-4 22-23 0.0 24-25 0.0 26-27 2.3262522215708716E-4 28-29 0.018713406760192345 30-31 0.004445726467890999 32-33 0.008503744232186852 34-35 0.009951190058942062 36-37 0.10266526471199447 38-39 0.10517244766190975 40-41 0.11848377981867639 42-43 0.10468134997068922 44-45 0.10000299828064113 46-47 0.10147629135430268 48-49 0.1033372931315594 50-51 0.10121781888523926 52-53 0.0795578259777238 54-55 0.06728038369721087 56-57 0.037969605705417894 58-59 0.015870209600494616 60-61 0.00951178686153423 62-63 0.0022745577277581857 64-65 0.0050660603936432316 66-67 0.42087072137598336 68-69 0.8113709276370137 70-71 0.8971579401191662 72-73 0.27930535006994267 74-75 0.1475619325883123 76-77 0.014784625230428207 78-79 0.008064341034779023 80-81 0.0011372788638790929 82-83 0.0 84-85 0.0 86-87 1.0338898762537208E-4 88-89 6.978756664712615E-4 90-91 0.003127516875667505 92-93 0.0020419325056010985 94-95 0.2229841990610212 96-97 0.22973033050357675 98-99 0.013466415638204712 100-101 0.007314770874495074 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1934442.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.02648046281986 #Duplication Level Percentage of deduplicated Percentage of total 1 77.83553856279353 45.943578962897945 2 13.137250110712298 15.508912739902753 3 4.152009629018651 7.352355457461281 4 1.7491222265192472 4.129781157228893 5 0.8819151708361037 2.6028174300610862 6 0.5098431797084974 1.8056549091699343 7 0.32897279534610374 1.359267439410722 8 0.22717315634857457 1.0727385499909008 9 0.1686774576629455 0.8960792993333981 >10 0.9077294960729312 9.82677558703184 >50 0.06606486688525716 2.688468507566325 >100 0.0332296660631682 3.472694078254802 >500 0.001236841016410212 0.5241857516115968 >1k 9.718036557508808E-4 1.1712829453788376 >5k 8.834578688644371E-5 0.4130596238077456 >10k+ 1.7669157377288742E-4 1.2323475608918877 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12380 0.6399778334010531 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11252 0.5816664443803432 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7921 0.4094720854902861 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4439 0.22947185803451328 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3216 0.16624949210159828 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2954 0.15270553472267454 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2387 0.12339475673088156 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1964 0.10152798584811538 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.169449381268604E-5 2 0.0 0.0 0.0 0.0 5.169449381268604E-5 3 0.0 0.0 0.0 0.0 5.169449381268604E-5 4 0.0 0.0 0.0 0.0 5.169449381268604E-5 5 0.0 0.0 0.0 0.0 1.0338898762537208E-4 6 0.0 0.0 0.0 5.169449381268604E-5 1.0338898762537208E-4 7 0.0 0.0 0.0 5.169449381268604E-5 1.0338898762537208E-4 8 0.0 0.0 0.0 5.169449381268604E-5 1.0338898762537208E-4 9 0.0 0.0 0.0 5.169449381268604E-5 1.0338898762537208E-4 10-11 0.0 0.0 0.0 5.169449381268604E-5 1.0338898762537208E-4 12-13 5.169449381268604E-5 0.0 0.0 1.0338898762537208E-4 4.652504443141743E-4 14-15 5.169449381268604E-5 0.0 0.0 1.809307283444011E-4 5.169449381268604E-4 16-17 5.169449381268604E-5 0.0 0.0 4.135559505014883E-4 5.169449381268604E-4 18-19 1.292362345317151E-4 0.0 0.0 4.910976912205174E-4 6.203339257522324E-4 20-21 2.0677797525074415E-4 0.0 0.0 6.461811726585754E-4 6.203339257522324E-4 22-23 2.0677797525074415E-4 0.0 0.0 0.0011114316169727498 6.203339257522324E-4 24-25 2.0677797525074415E-4 0.0 0.0 0.0017576127896313251 6.203339257522324E-4 26-27 2.0677797525074415E-4 0.0 0.0 0.002558877443727959 6.203339257522324E-4 28-29 3.3601420978245924E-4 0.0 0.0 0.004730046183860772 6.203339257522324E-4 30-31 3.618614566888022E-4 0.0 0.0 0.009279161639377143 6.203339257522324E-4 32-33 3.618614566888022E-4 0.0 0.0 0.01866171226637966 6.203339257522324E-4 34-35 3.8770870359514526E-4 0.0 0.0 0.03246414211436683 6.203339257522324E-4 36-37 5.169449381268604E-4 0.0 0.0 0.054822010688353545 6.203339257522324E-4 38-39 5.686394319395464E-4 0.0 0.0 0.08643319365481106 6.203339257522324E-4 40-41 5.686394319395464E-4 0.0 0.0 0.11949182244802375 6.203339257522324E-4 42-43 5.944866788458894E-4 0.0 0.0 0.15570381536381034 6.203339257522324E-4 44-45 7.237229133776044E-4 0.0 0.0 0.19889456494430952 6.203339257522324E-4 46-47 7.754174071902905E-4 0.0 0.0 0.2549830907310739 6.461811726585754E-4 48-49 7.754174071902905E-4 0.0 0.0 0.31675801083723365 6.720284195649185E-4 50-51 7.754174071902905E-4 0.0 0.0 0.3817896840535927 6.720284195649185E-4 52-53 7.754174071902905E-4 0.0 0.0 0.444882813751976 6.720284195649185E-4 54-55 7.754174071902905E-4 0.0 0.0 0.5187025509164916 6.720284195649185E-4 56-57 7.754174071902905E-4 5.169449381268604E-5 0.0 0.6028870340904509 7.237229133776044E-4 58-59 7.754174071902905E-4 5.169449381268604E-5 0.0 0.6852622099809661 7.237229133776044E-4 60-61 7.754174071902905E-4 5.169449381268604E-5 0.0 0.7680767890688891 7.237229133776044E-4 62-63 7.754174071902905E-4 5.169449381268604E-5 0.0 0.8509172154037186 7.754174071902905E-4 64-65 7.754174071902905E-4 5.169449381268604E-5 0.0 0.9447427216737436 8.271119010029766E-4 66-67 8.271119010029766E-4 5.169449381268604E-5 0.0 1.041204647128216 8.788063948156626E-4 68-69 8.271119010029766E-4 5.169449381268604E-5 0.0 1.1604121498602697 8.788063948156626E-4 70-71 8.788063948156626E-4 5.169449381268604E-5 5.169449381268604E-5 1.3129626011015063 9.046536417220057E-4 72-73 9.563481355346916E-4 5.169449381268604E-5 5.169449381268604E-5 1.4799358161164822 0.0010338898762537207 74-75 0.0010597371231600636 5.169449381268604E-5 5.169449381268604E-5 1.6609699334485086 0.0011372788638790929 76-77 0.0010855843700664067 5.169449381268604E-5 5.169449381268604E-5 1.8656025872060265 0.0011372788638790929 78-79 0.0010855843700664067 5.169449381268604E-5 5.169449381268604E-5 2.103216328016038 0.0011372788638790929 80-81 0.0012148206045981217 5.169449381268604E-5 5.169449381268604E-5 2.3718726123605673 0.0011372788638790929 82-83 0.001318209592223494 5.169449381268604E-5 5.169449381268604E-5 2.642751759939042 0.0011889733576917788 84-85 0.0013699040860361798 7.754174071902906E-5 5.169449381268604E-5 2.9092885700372513 0.0011889733576917788 86-87 0.001395751332942523 1.0338898762537208E-4 5.169449381268604E-5 3.1836054014542694 0.0012148206045981217 88-89 0.001395751332942523 1.0338898762537208E-4 5.169449381268604E-5 3.492790168947945 0.0012406678515044648 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10645 0.0 30.764194 1 GTATCAA 15005 0.0 26.625927 1 ATCAACG 18215 0.0 21.845459 3 TATCAAC 18310 0.0 21.78724 2 GTACATG 10455 0.0 21.758574 1 TCAACGC 18400 0.0 21.625818 4 CAACGCA 18555 0.0 21.368574 5 AACGCAG 18750 0.0 21.146341 6 TACATGG 10540 0.0 21.081944 2 GAGTACT 10420 0.0 20.981548 12-13 AGTACTT 10785 0.0 20.09576 12-13 GTACTTT 11030 0.0 19.799715 14-15 ACATGGG 10640 0.0 19.678488 3 AGAGTAC 17120 0.0 18.816008 10-11 ACGCAGA 21105 0.0 18.719393 7 CGCAGAG 21230 0.0 18.586863 8 ACTTTTT 12635 0.0 17.715544 16-17 TACTTTT 12180 0.0 17.658022 14-15 GCAGAGT 21780 0.0 17.508505 9 TGCACCG 325 1.0375516E-8 17.49077 5 >>END_MODULE