FastQCFastQC Report
Fri 27 May 2016
SRR939382_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939382_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1920251
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83140.43296423228005093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63630.33136293120014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45570.23731272630505076No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28170.1466995720871907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA128500.030.9343071
GGTATCA88050.028.4948581
GTACATG93200.028.2717251
TACATGG97700.026.2602842
TATCAAC158550.024.9521392
ACATGGG102150.024.6454753
ATCAACG161300.024.5307773
TCAACGC164450.024.35874
CAACGCA164250.024.2421745
AACGCAG168900.023.8386236
CGACGCA1702.602099E-622.8509465
ATCGACG1753.6098736E-622.0052263
CATGGGA67850.021.647594
ACGCAGA183000.021.499377
GAGTACT85550.021.36103412-13
CGCAGAG185450.021.2142038
AGTACTT89050.021.08850512-13
AGAGTAC139450.020.50680510-11
GTGTAGC11050.020.06121
CATGGGG36850.020.0587044
Produced by FastQC (version 0.11.5)