FastQCFastQC Report
Fri 27 May 2016
SRR939382_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939382_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1920251
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120450.6272617485943244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108250.5637283875909972No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76960.4007809395750868No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43180.22486643673144813No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30100.15675034149181538No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28230.14701203123966608No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23510.12243191124493621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102750.029.2481961
GTATCAA145900.027.8870581
GTACATG105200.023.0612221
ATCAACG180800.022.3913653
TACATGG106750.022.1441692
TATCAAC183300.022.1412182
TCAACGC183700.022.0160644
CAACGCA186100.021.7066695
AACGCAG189450.021.4479686
GAGTACT102250.021.11711312-13
ATTCGCG1351.027729E-921.07091980-81
ACATGGG107250.020.4444143
AGTACTT108850.020.27219412-13
AGAGTAC169650.019.39368410-11
GTACTTT110800.019.3592414-15
TCGCGCT1352.3168468E-819.34170760-61
ACGCAGA212900.019.0187577
CATGGGG35600.018.9342274
CGCAGAG213700.018.8588128
CATGGGA73600.018.1878154
Produced by FastQC (version 0.11.5)