FastQCFastQC Report
Fri 27 May 2016
SRR939381_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939381_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11840
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.2702702702702703No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.2195945945945946No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.10135135135135136No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA200.001766324673.707591
GCGTGTG250.00459072757.931587
TATCAAC300.00927864948.4889872
ATCAACG300.00994608247.6493533
AGAGTAC351.7637669E-434.6349910-11
AGTACTT300.00385371431.69762412-13
GAGTACT404.3356465E-429.71652212-13
GTACTTT404.5609058E-429.46199214-15
CAGAGTA350.007435769327.7079939
TACTTTT500.001678680723.56959314-15
Produced by FastQC (version 0.11.5)