FastQCFastQC Report
Fri 27 May 2016
SRR939381_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939381_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11840
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.6587837837837838No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.5067567567567568No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.37162162162162166No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC200.16891891891891891No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.1266891891891892No Hit
ACGTAACACAGTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAAC140.11824324324324326No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGGGC200.00203780371.122898
AAGAGGG301.2598964E-463.220347
AGAAGAG405.2187714E-447.4152575
GAAGAGG405.2187714E-447.4152576
GGTATCA1200.00443002219.8404251
AGTACTT650.00742746818.23663512-13
GAGTACT650.00742746818.23663512-13
GTATCAA1350.00874857117.6359352
AGAGTAC1400.003384024811.85381310-11
Produced by FastQC (version 0.11.5)