FastQCFastQC Report
Fri 27 May 2016
SRR939380_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939380_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11662
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.6088149545532499No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.5745155204939119No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.3858686331675527No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC350.3001200480192077No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.24009603841536614No Hit
ACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATA190.16292231178185562No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.16292231178185562No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.1371977362373521No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTTGT150.00993475947.81601766-67
GAGTACT401.04724495E-535.55418412-13
GGTATCA550.00250094234.4767841
GTATCAA851.519051E-533.462761
AGTACTT404.4125336E-429.62848712-13
AACGCAG908.5235795E-426.3364356
TCAACGC908.5235795E-426.3364354
TATCAAC908.5235795E-426.3364352
CAACGCA908.5235795E-426.3364355
GTACTTT559.215884E-525.8575914-15
CTTTTTT559.215884E-525.8575916-17
ATCAACG950.001168675524.9503043
ACGCAGA1050.002093438822.5740857
ACTTTTT652.861861E-421.87949616-17
TACTTTT652.861861E-421.87949614-15
CGCAGAG1100.002743264221.547998
CAGAGTA857.652142E-519.51994310-11
Produced by FastQC (version 0.11.5)