FastQCFastQC Report
Fri 27 May 2016
SRR939379_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939379_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765591
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142040.80448982805191No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT120610.6831140394349541No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110340.6249465476432537No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT83000.47009754807313814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73970.4189532003731329No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68800.3896712205714687No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT58480.3312205374857484No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC47710.2702211327538484No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT38010.21528202171397565No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35960.20367117865915718No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC33910.19206033560433872No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC29110.1648739713784223No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC29040.16447750356679433No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27210.1541127022056637No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA24880.14091598790433346No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23780.13468577943589427No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG22230.1259068493212754No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21090.11945008781762027No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA18480.10466750226977822No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18220.10319490754087443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1508.551433E-725.9667072
TTAGCGA1151.2224773E-424.95714
GAGTACT116450.023.88924412-13
GTACATG128700.023.8116471
TACATGG129550.023.3008862
GTACTTT128050.022.1476314-15
AGTACTT120700.021.7588112-13
ACATGGG131950.021.5134983
ATCACGC1604.7013993E-520.9432353
CATGGGG40850.020.492114
ACTTTTT149450.020.33381816-17
AGAGTAC208250.019.14531310-11
AGTACAT101950.019.102532
CATGGGA87200.018.9801794
TACTTTT139050.018.93022514-15
ATGGGAG34050.018.837625
GAGTACA124600.017.7965131
GTATCAA356900.016.7962381
ATGGGGG18900.016.3308835
ATGGGAT32050.015.9503175
Produced by FastQC (version 0.11.5)