FastQCFastQC Report
Fri 27 May 2016
SRR939378_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939378_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734383
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139260.8029368369039594No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT108570.6259863017568784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103650.5976188650373072No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT78790.4542825892550838No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74560.4298935125632574No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69450.4004305854012637No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT54560.31457872915036644No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC45470.2621681600892075No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT36170.20854678580221322No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT33740.194536039617547No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC32390.18675229173717686No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC29380.1693974168335368No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC27570.15896142893467013No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25250.1455849140587748No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23090.13313091745018257No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG21380.1232715034683804No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21030.1212534947586548No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA20900.12050394866647103No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA18450.10637788769839186No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18260.10528239725596941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG122750.026.1617981
TACATGG124700.025.2461572
ACATGGG130150.023.5786363
GAGTACT111100.023.47625712-13
GTATCGA1752.551331E-622.9025061
AGTACTT116350.022.41695212-13
CATGGGA85500.021.9152454
CATGGGG39600.021.7923134
GTACTTT122950.021.61655214-15
CGACGGT1404.249749E-420.7617057
GTGTAAG8800.019.925831
ACTTTTT147850.019.68484516-17
AGAGTAC195100.019.60209710-11
CTATACC2702.2038876E-819.4244544
ATGGGAG31050.019.3607035
TACTTTT137750.019.24236514-15
AGTACAT93950.018.287232
ATGGGAT33900.018.019055
TAATGCG1350.00839750217.9411539
GTATCAA331950.017.2355251
Produced by FastQC (version 0.11.5)