##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939378_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1734383 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.90179216470641 31.0 28.0 31.0 16.0 33.0 2 27.90415323489679 31.0 27.0 31.0 16.0 34.0 3 28.194590237565752 31.0 27.0 33.0 16.0 34.0 4 32.086693654169814 35.0 32.0 37.0 19.0 37.0 5 32.56363732808728 35.0 32.0 37.0 25.0 37.0 6 32.47364163509444 35.0 32.0 37.0 25.0 37.0 7 32.49789175747225 35.0 32.0 37.0 25.0 37.0 8 32.44646712980928 35.0 32.0 37.0 25.0 37.0 9 33.57543576015217 37.0 32.0 39.0 25.0 39.0 10-11 32.698865533160784 37.0 32.0 39.0 18.0 39.0 12-13 32.46129689924313 37.0 31.5 39.0 17.0 39.0 14-15 33.26159100959822 37.0 32.0 39.0 17.0 41.0 16-17 33.23740171576866 37.0 31.5 39.0 17.5 40.5 18-19 33.32479792525642 37.0 32.0 39.0 18.0 40.0 20-21 33.283504566177136 37.0 31.5 39.0 18.0 40.0 22-23 33.4785799906941 37.0 32.0 39.0 18.0 41.0 24-25 33.51463575231077 37.0 32.0 40.0 18.5 41.0 26-27 33.603262370537536 37.0 32.0 40.0 19.0 41.0 28-29 33.21719885400168 37.0 31.0 40.0 17.5 41.0 30-31 32.903009888819255 37.0 31.0 39.0 16.5 41.0 32-33 32.7313482662134 36.0 31.0 39.0 16.5 41.0 34-35 32.247515398847895 36.0 30.0 39.0 15.5 40.0 36-37 31.84766484680719 35.5 30.0 39.0 14.0 40.0 38-39 31.43803790742875 35.0 30.0 39.0 10.5 40.0 40-41 31.31768559770247 35.0 29.5 39.0 10.5 40.0 42-43 30.989718245623948 35.0 29.0 39.0 9.0 40.0 44-45 30.57939970583199 35.0 27.5 38.5 8.0 40.0 46-47 29.900113758033836 35.0 26.5 38.0 4.5 40.0 48-49 29.46856605490252 35.0 25.5 38.0 2.0 40.0 50-51 28.761999223931507 33.5 24.0 38.0 2.0 39.5 52-53 28.913187283316315 34.0 25.0 38.0 2.0 39.5 54-55 29.08488695980069 34.0 26.0 38.0 2.0 40.0 56-57 28.621599150821936 34.0 24.0 38.0 2.0 40.0 58-59 28.23601274920245 34.0 23.0 38.0 2.0 40.0 60-61 27.594279637196628 33.0 22.5 38.0 2.0 40.0 62-63 27.093502992130343 33.0 22.0 37.0 2.0 39.0 64-65 26.59834880761631 32.0 20.0 36.5 2.0 39.0 66-67 26.194898992898338 32.0 19.0 36.0 2.0 39.0 68-69 25.504243295742633 31.0 17.5 35.5 2.0 38.5 70-71 24.85501529938889 31.0 13.0 35.0 2.0 38.0 72-73 24.38916923194012 30.5 10.0 35.0 2.0 37.5 74-75 23.683669351002635 30.0 7.5 34.0 2.0 37.0 76-77 22.919865162423754 29.0 4.5 34.0 2.0 36.0 78-79 22.410920771248335 29.0 2.0 34.0 2.0 36.0 80-81 21.8368883920103 29.0 2.0 34.0 2.0 35.0 82-83 21.092715392159633 27.5 2.0 33.5 2.0 35.0 84-85 20.41626013400731 26.5 2.0 33.0 2.0 35.0 86-87 19.68904388477055 26.0 2.0 33.0 2.0 35.0 88-89 18.86457057062944 25.0 2.0 32.0 2.0 34.0 90-91 18.031598268663842 23.5 2.0 32.0 2.0 34.0 92-93 17.19453921077409 20.0 2.0 31.5 2.0 34.0 94-95 16.100781372972406 12.5 2.0 31.0 2.0 34.0 96-97 14.952385661067941 2.0 2.0 31.0 2.0 34.0 98-99 13.898324072595269 2.0 2.0 30.5 2.0 34.0 100-101 12.45552654748115 2.0 2.0 28.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.850780721362522 1101 2 3.6923325495186106 1101 3 3.2344360435404518 1101 4 3.158798570738359 1101 5 2.297928106439578 1101 6 2.573274937710579 1101 7 2.1910387283182686 1101 8 2.2473076028170027 1101 9 2.511586489823266 1101 10-11 3.385404197604444 1101 12-13 3.3386435055952646 1101 14-15 3.0054035884774493 1101 16-17 2.9594022214666396 1101 18-19 2.9562843291674668 1101 20-21 2.8949100099506637 1101 22-23 2.872775027613862 1101 24-25 3.053248352535313 1101 26-27 2.896902525613598 1101 28-29 3.1405535012726773 1101 30-31 3.2708000522582665 1101 32-33 3.012918358209781 1101 34-35 3.3561786953030968 1101 36-37 3.388693900537188 1101 38-39 3.3142612240485114 1101 40-41 3.2049143014482837 1101 42-43 3.003162596883911 1101 44-45 3.132631476172609 1101 46-47 3.054496112895059 1101 48-49 2.9023542477375237 1101 50-51 3.0814082845610535 1101 52-53 3.0995963664725856 1101 54-55 3.18813870300853 1101 56-57 3.4414752272088442 1101 58-59 3.212067739567228 1101 60-61 3.020396068386713 1101 62-63 3.047551888317532 1101 64-65 3.2144724494739556 1101 66-67 2.8983794786072288 1101 68-69 3.1589877169502323 1101 70-71 2.6715733222406115 1101 72-73 2.7252320094423403 1101 74-75 2.9386239587363754 1101 76-77 2.9222582638347134 1101 78-79 2.583475115383564 1101 80-81 2.6319942374310656 1101 82-83 2.3618122336638407 1101 84-85 2.2618752494188605 1101 86-87 2.2309071217924874 1101 88-89 2.2798502445939093 1101 90-91 2.281413420670372 1101 92-93 1.9191096221237807 1101 94-95 1.89583246103801 1101 96-97 1.7114832907642725 1101 98-99 1.532726546344815 1101 100-101 1.4770925727710473 1102 1 3.5415372946902615 1102 2 2.561392093850717 1102 3 2.751227537429159 1102 4 3.0070801095105075 1102 5 1.8356218507126627 1102 6 2.421569095097709 1102 7 2.224541151092339 1102 8 2.3132495727186964 1102 9 2.446862701598569 1102 10-11 1.771331918177836 1102 12-13 3.1375629638878237 1102 14-15 3.286552154226257 1102 16-17 3.0469035803944067 1102 18-19 2.8996461611574063 1102 20-21 2.9005612081366863 1102 22-23 2.7672843770369084 1102 24-25 2.9591872551429006 1102 26-27 2.6572905577308816 1102 28-29 2.8803824122667905 1102 30-31 3.109643352356116 1102 32-33 2.8638852353161184 1102 34-35 3.0721734692551728 1102 36-37 3.182174735773021 1102 38-39 3.148159968399259 1102 40-41 2.8226037553922794 1102 42-43 2.621363104734627 1102 44-45 2.8941343143903175 1102 46-47 2.883211518476756 1102 48-49 2.7932415693297195 1102 50-51 2.7983018921648366 1102 52-53 2.8541322732268455 1102 54-55 2.907732208886859 1102 56-57 2.8787781826826055 1102 58-59 2.956169022395038 1102 60-61 3.2700563364449238 1102 62-63 3.2998712868172753 1102 64-65 3.3124797760359463 1102 66-67 2.8713285654077687 1102 68-69 2.9314857367983436 1102 70-71 2.8959191926532597 1102 72-73 2.8703776282592166 1102 74-75 2.9051535678310962 1102 76-77 2.889333385361681 1102 78-79 2.657505132156615 1102 80-81 2.646351309291166 1102 82-83 2.5602666474612263 1102 84-85 2.3722206694440224 1102 86-87 2.060053016277571 1102 88-89 1.9687426602239064 1102 90-91 1.983518011516633 1102 92-93 1.8536845301049567 1102 94-95 1.875668399630003 1102 96-97 1.8604955444100835 1102 98-99 1.2082417741011096 1102 100-101 0.588052907610848 1103 1 3.82588619928363 1103 2 3.686294100975374 1103 3 3.2772040556462265 1103 4 2.9287924485428043 1103 5 2.4308548335773636 1103 6 2.479010453086822 1103 7 2.3871850661190663 1103 8 2.4144471513339596 1103 9 2.6797302805596885 1103 10-11 3.480732124923584 1103 12-13 2.6908264148510384 1103 14-15 3.175995535480027 1103 16-17 2.702003244912923 1103 18-19 2.587828508605554 1103 20-21 2.710203803141745 1103 22-23 2.6129535242683204 1103 24-25 2.8546423071559843 1103 26-27 2.733320059350646 1103 28-29 2.233096118395764 1103 30-31 2.793630860185935 1103 32-33 2.5650728945545396 1103 34-35 2.4395033651521523 1103 36-37 2.66607484260447 1103 38-39 3.1097867510049504 1103 40-41 2.8986727663943768 1103 42-43 2.9998502580608175 1103 44-45 2.8410074049703162 1103 46-47 2.7221930122340297 1103 48-49 2.376664129018909 1103 50-51 2.454307750601643 1103 52-53 2.4821678907548907 1103 54-55 2.4976182153121194 1103 56-57 2.5907055488262607 1103 58-59 2.6817834473305773 1103 60-61 2.6022656338552217 1103 62-63 2.6994520070072703 1103 64-65 2.780012790988504 1103 66-67 2.281454567913311 1103 68-69 2.335297563577683 1103 70-71 2.1824062873758656 1103 72-73 2.1968832839852688 1103 74-75 2.408456984516018 1103 76-77 2.5135259048545784 1103 78-79 1.8965557493195888 1103 80-81 1.766589768228311 1103 82-83 2.061781333903241 1103 84-85 1.907209529959811 1103 86-87 2.1784807686284395 1103 88-89 2.0743130508119805 1103 90-91 1.6444816369455921 1103 92-93 1.5753053851024 1103 94-95 1.5022330432241535 1103 96-97 1.4289858392725652 1103 98-99 1.1855554627450804 1103 100-101 1.1716874249286864 1104 1 3.560095096233727 1104 2 3.490003955800809 1104 3 2.7948631977104306 1104 4 2.704974495846546 1104 5 1.7306785553783115 1104 6 2.103970571965341 1104 7 2.0968366617281404 1104 8 2.1682163725776675 1104 9 2.3040413751598976 1104 10-11 3.0946464478914706 1104 12-13 3.141967124188689 1104 14-15 2.9965366804142235 1104 16-17 3.027629870205196 1104 18-19 2.8082243332888694 1104 20-21 2.4841266486506512 1104 22-23 2.282031997203788 1104 24-25 2.4930964493294496 1104 26-27 2.499395975523811 1104 28-29 2.501376604690499 1104 30-31 2.102450233305696 1104 32-33 2.2752362068364747 1104 34-35 2.840479086836556 1104 36-37 3.140391036160846 1104 38-39 3.2920372845742882 1104 40-41 3.0757340194264344 1104 42-43 3.071408644751088 1104 44-45 2.645476275842899 1104 46-47 2.6099569742686626 1104 48-49 2.4070688404544107 1104 50-51 2.323808708476978 1104 52-53 2.686424062312909 1104 54-55 2.3565650623361485 1104 56-57 2.6967248665859245 1104 58-59 2.700967177289858 1104 60-61 3.094951545439322 1104 62-63 3.0491894107644733 1104 64-65 3.064383397691447 1104 66-67 2.9602469165653638 1104 68-69 2.7842504565068893 1104 70-71 2.74835509385591 1104 72-73 2.626370547521173 1104 74-75 2.686162891292529 1104 76-77 2.654131442320981 1104 78-79 2.4693306012677496 1104 80-81 2.504118085645615 1104 82-83 2.585236532289059 1104 84-85 2.321950612249676 1104 86-87 2.32856380877179 1104 88-89 2.3314759915985306 1104 90-91 2.2889784063926477 1104 92-93 2.1856504387507023 1104 94-95 2.4220166289558183 1104 96-97 2.4191608791536883 1104 98-99 2.4419746843529975 1104 100-101 2.1959376962659984 1105 1 3.4118512187507974 1105 2 3.1609198680428783 1105 3 2.905349514264767 1105 4 2.654747241655503 1105 5 -0.15511527724693508 1105 6 1.6931202038357966 1105 7 1.938287381304626 1105 8 2.026007150432065 1105 9 2.1619567164860527 1105 10-11 2.565970898662947 1105 12-13 3.000085993330309 1105 14-15 2.82005730382474 1105 16-17 2.5872535105726584 1105 18-19 2.68223208884789 1105 20-21 2.5688731842290977 1105 22-23 2.377911022346332 1105 24-25 2.1075183784320686 1105 26-27 2.3896214502366817 1105 28-29 2.3656546926412076 1105 30-31 2.409654524813874 1105 32-33 2.4574699709518057 1105 34-35 2.7814741858571033 1105 36-37 2.7164018168327395 1105 38-39 2.7609535082250325 1105 40-41 2.5912562591962853 1105 42-43 2.68178263174606 1105 44-45 2.4138527244493453 1105 46-47 2.5972500172504738 1105 48-49 2.4921772032603506 1105 50-51 2.5560832523003327 1105 52-53 2.5394554775170697 1105 54-55 2.3958870811541324 1105 56-57 2.710912127005141 1105 58-59 2.8413887653258314 1105 60-61 2.97194289272673 1105 62-63 2.9463726451143337 1105 64-65 2.820251968399756 1105 66-67 2.7268086681650416 1105 68-69 2.9092684366508763 1105 70-71 2.8697151341907485 1105 72-73 2.8556296172989626 1105 74-75 2.8771705949500976 1105 76-77 2.7939113060426095 1105 78-79 2.684886576977874 1105 80-81 2.5702575819050004 1105 82-83 2.6551070011074103 1105 84-85 2.3973908477339556 1105 86-87 2.439381182410308 1105 88-89 2.407962411955758 1105 90-91 2.314616608823119 1105 92-93 2.4255871189858915 1105 94-95 2.5189199662188297 1105 96-97 2.5430911532674703 1105 98-99 2.2981631904069317 1105 100-101 2.201872626680986 1106 1 3.2452863863790675 1106 2 2.732069723783887 1106 3 2.2983021301285227 1106 4 1.5703134591239554 1106 5 1.6726487347387007 1106 6 2.0759202035351763 1106 7 2.081424313484483 1106 8 0.6898525186250453 1106 9 2.025245490739337 1106 10-11 1.0752389468189207 1106 12-13 1.8895518179663924 1106 14-15 1.8574932440408674 1106 16-17 2.1906649218757437 1106 18-19 2.248517572087316 1106 20-21 2.0308472814924983 1106 22-23 2.5003677014360264 1106 24-25 2.896030342646519 1106 26-27 2.5799734211248477 1106 28-29 2.3391849156320106 1106 30-31 2.4541294318665976 1106 32-33 2.609815579263291 1106 34-35 2.789289874050265 1106 36-37 1.9156873985245149 1106 38-39 2.931293349183168 1106 40-41 2.2538901374746203 1106 42-43 2.098062440823668 1106 44-45 2.2041368308590457 1106 46-47 2.5732509431534396 1106 48-49 2.577563944359998 1106 50-51 2.5178995251952045 1106 52-53 2.7016181373236385 1106 54-55 2.636495967567935 1106 56-57 2.5961042932047214 1106 58-59 2.7014624317287073 1106 60-61 2.7835787573374695 1106 62-63 2.981479548577674 1106 64-65 2.7428650439232243 1106 66-67 2.28384789804225 1106 68-69 2.614835052437975 1106 70-71 2.5425155322087747 1106 72-73 2.7108452993294563 1106 74-75 2.6329674464291024 1106 76-77 2.6872167721017277 1106 78-79 2.6174760314541885 1106 80-81 2.6971340948582494 1106 82-83 2.6697835400641026 1106 84-85 2.5553087835715864 1106 86-87 2.703410829342161 1106 88-89 2.846249326136128 1106 90-91 2.428167860032165 1106 92-93 2.563394848321611 1106 94-95 2.7943828613340163 1106 96-97 2.5430919048120817 1106 98-99 2.236948378665099 1106 100-101 2.13768094673077 1107 1 2.9412032612599397 1107 2 3.1890776437658346 1107 3 0.1922956453824547 1107 4 1.1840016059470706 1107 5 1.6441625324355655 1107 6 1.793080111248365 1107 7 1.8678867880076524 1107 8 1.7547898310627872 1107 9 2.154305867143833 1107 10-11 1.4813620527873965 1107 12-13 2.2370398627625505 1107 14-15 2.453677308323698 1107 16-17 1.189546958459239 1107 18-19 0.2200728398336409 1107 20-21 -1.0825944375367555 1107 22-23 0.4812050827512735 1107 24-25 1.3469109299049578 1107 26-27 1.2217297468359334 1107 28-29 2.09969458253979 1107 30-31 2.7998754687520773 1107 32-33 1.455778802241106 1107 34-35 1.1047183806154202 1107 36-37 1.3177594654954525 1107 38-39 2.105882921964568 1107 40-41 2.39864240842504 1107 42-43 1.572083315676899 1107 44-45 1.0695102965669 1107 46-47 1.9245657962630673 1107 48-49 1.9089992254299055 1107 50-51 2.128015665258701 1107 52-53 1.7298781151903277 1107 54-55 2.225825245343099 1107 56-57 2.560867909935336 1107 58-59 2.6176675447199678 1107 60-61 2.394398957823892 1107 62-63 2.3177936746322665 1107 64-65 1.5761268680046179 1107 66-67 2.19657883105722 1107 68-69 2.633975474012928 1107 70-71 1.1671484491668593 1107 72-73 2.1479253984641424 1107 74-75 2.4924655897139694 1107 76-77 2.662023584142183 1107 78-79 2.5630220019344243 1107 80-81 2.7372607539592906 1107 82-83 2.372028717908144 1107 84-85 2.3303296448672945 1107 86-87 2.676478557642099 1107 88-89 2.5072290135210693 1107 90-91 2.805250367619724 1107 92-93 2.697923024163128 1107 94-95 2.795939450273581 1107 96-97 2.432111938301807 1107 98-99 2.2452002539221283 1107 100-101 1.916898390947571 1108 1 2.39450751145435 1108 2 2.1798609012740755 1108 3 1.8437124046850606 1108 4 1.6547220621333842 1108 5 1.8806163006540473 1108 6 -0.08859405999628933 1108 7 0.48904457674650104 1108 8 -0.850792595254763 1108 9 0.8436232965981105 1108 10-11 2.5454507684218655 1108 12-13 1.670984918007342 1108 14-15 1.8864251472668627 1108 16-17 -0.45223584871866507 1108 18-19 -0.07224081719944309 1108 20-21 1.1001959851781535 1108 22-23 -0.8796222447858426 1108 24-25 0.865511827559736 1108 26-27 1.1315202012799261 1108 28-29 1.8805832641072513 1108 30-31 0.6964911165347445 1108 32-33 0.8471225512495693 1108 34-35 1.143033894634943 1108 36-37 0.13350519083624945 1108 38-39 1.5385720342682738 1108 40-41 0.0355074857799913 1108 42-43 1.1754725385870763 1108 44-45 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-3.0186746083913896 2312 46-47 -0.6828677675683714 2312 48-49 0.006026781839921114 2312 50-51 -1.2520452151820365 2312 52-53 -1.3381097810751932 2312 54-55 -1.118654428445236 2312 56-57 -0.23670462461354091 2312 58-59 -0.6594805839041733 2312 60-61 -0.41934462078836177 2312 62-63 -0.8984933937005977 2312 64-65 -2.6935339483514475 2312 66-67 0.35014302426217725 2312 68-69 -0.3104775839630314 2312 70-71 -3.4125452364254585 2312 72-73 -3.6717460125356816 2312 74-75 -1.338144144954807 2312 76-77 -0.3455968071877784 2312 78-79 -0.509206335895172 2312 80-81 -2.3736328654811025 2312 82-83 -0.3285976581035186 2312 84-85 0.9290433000710756 2312 86-87 1.2885621364610778 2312 88-89 1.7189901017201876 2312 90-91 2.032128203624975 2312 92-93 2.1698575544327987 2312 94-95 1.6374332433627732 2312 96-97 1.3365288711265944 2312 98-99 0.6233838151695057 2312 100-101 1.4667914108861755 2313 1 -8.100383066631892 2313 2 -4.166067140439797 2313 3 -1.88740943641805 2313 4 0.2730248584830619 2313 5 0.562656746797991 2313 6 -2.6842114080736934 2313 7 -0.61113495889931 2313 8 -2.115076481181891 2313 9 0.6899709440608319 2313 10-11 -1.353826917892711 2313 12-13 -2.0005147607821385 2313 14-15 -3.6176138700072045 2313 16-17 -3.8264874567320284 2313 18-19 -1.1492946215654847 2313 20-21 -2.831344106956859 2313 22-23 -1.6391628388791304 2313 24-25 -2.7149342949908117 2313 26-27 -1.854336375549991 2313 28-29 -2.6066264253987583 2313 30-31 -4.232860216471078 2313 32-33 -5.682234877982516 2313 34-35 -4.601122997273617 2313 36-37 -1.7934671879112365 2313 38-39 -1.391982857792879 2313 40-41 -3.2357168289649607 2313 42-43 -2.6368335675500063 2313 44-45 -3.0733865336746717 2313 46-47 -1.4321663053494547 2313 48-49 -2.5023252760662906 2313 50-51 -2.8526079926225236 2313 52-53 -0.22816840411927686 2313 54-55 -1.527435260156718 2313 56-57 -2.1019893610604505 2313 58-59 -1.1582989498731138 2313 60-61 -1.34032077627419 2313 62-63 -3.83665147259725 2313 64-65 -5.22316014724424 2313 66-67 -3.854963157305658 2313 68-69 -2.533051285962401 2313 70-71 -1.5336650888228647 2313 72-73 -1.299740289247044 2313 74-75 -2.5233150397813375 2313 76-77 -2.3023983664340264 2313 78-79 -2.5565754861210976 2313 80-81 -3.467844182688612 2313 82-83 -1.0734299596939714 2313 84-85 -0.931385341338224 2313 86-87 -2.770102044944096 2313 88-89 -3.527319833462526 2313 90-91 -3.4661492302385106 2313 92-93 -1.8438459012911537 2313 94-95 -0.7417544884483842 2313 96-97 -0.8772601218507425 2313 98-99 -0.7371717845180417 2313 100-101 -0.43155969187768584 2314 1 -8.346764187251154 2314 2 -3.5905968781314144 2314 3 -25.735485060996037 2314 4 -13.971925720448112 2314 5 -6.785854966822267 2314 6 -5.293537352473873 2314 7 -4.569563021641475 2314 8 -3.8089518685613655 2314 9 -3.5613986959430655 2314 10-11 -14.979398887191664 2314 12-13 -13.425240279129685 2314 14-15 -4.944502540786129 2314 16-17 -3.2492759010085166 2314 18-19 -3.4982148594425126 2314 20-21 -4.649319692567193 2314 22-23 -4.920592702049426 2314 24-25 -5.6268582086437675 2314 26-27 -3.981594744289545 2314 28-29 -3.8162236355597514 2314 30-31 -4.076566233557475 2314 32-33 -4.4609115609540275 2314 34-35 -3.5828166813566718 2314 36-37 -4.392895253951746 2314 38-39 -1.2399847406797626 2314 40-41 -2.807168286906517 2314 42-43 -4.899165039514262 2314 44-45 -6.11830912374673 2314 46-47 -4.966539121664788 2314 48-49 -3.776101317131694 2314 50-51 -2.96047017623415 2314 52-53 -1.2071190386512178 2314 54-55 -3.595415439897966 2314 56-57 -3.6621998052398723 2314 58-59 -1.4475126843926596 2314 60-61 -4.534340584281924 2314 62-63 -5.444465830128067 2314 64-65 -4.669299027586209 2314 66-67 -5.760441858679137 2314 68-69 -5.168996567271638 2314 70-71 -5.925710551400016 2314 72-73 -5.190828523418613 2314 74-75 -4.421103637195525 2314 76-77 -4.173607913933338 2314 78-79 -5.54915773166632 2314 80-81 -7.789459733562602 2314 82-83 -7.217884985037081 2314 84-85 -5.790031000968252 2314 86-87 -5.243892645870558 2314 88-89 -4.220060117111238 2314 90-91 -6.280874527125388 2314 92-93 -5.883480528881478 2314 94-95 -6.322650301569505 2314 96-97 -5.859656825762068 2314 98-99 -6.630904117728706 2314 100-101 -5.8448689126989475 2315 1 -25.244880028144383 2315 2 -25.32541892977133 2315 3 -13.434593530535412 2315 4 -29.664179733009174 2315 5 -15.613456637897507 2315 6 -11.617735903088807 2315 7 -8.608452642124071 2315 8 -8.034260840169885 2315 9 -5.078350528619868 2315 10-11 -30.313313751113505 2315 12-13 -30.060271681781465 2315 14-15 -24.382492024501026 2315 16-17 -14.182217993004755 2315 18-19 -8.538044693050455 2315 20-21 -6.471279825664247 2315 22-23 -3.106148570980551 2315 24-25 -5.06522745861966 2315 26-27 -5.11572455850737 2315 28-29 -18.01205807924495 2315 30-31 -16.92184064514011 2315 32-33 -9.24494224710482 2315 34-35 -6.898416701680002 2315 36-37 -4.8187327357670355 2315 38-39 -5.140585263901755 2315 40-41 -9.118798518478393 2315 42-43 -8.275273620002416 2315 44-45 -4.881653306557137 2315 46-47 -3.9716365440239763 2315 48-49 -4.751440618195872 2315 50-51 -3.2945885326576168 2315 52-53 -7.117336850803529 2315 54-55 -7.1584346050032295 2315 56-57 -7.629091445612371 2315 58-59 -7.6357064382875315 2315 60-61 -5.099253136943712 2315 62-63 -3.598487878864649 2315 64-65 -4.928340575311292 2315 66-67 -7.392147131027279 2315 68-69 -7.289061290848458 2315 70-71 -8.043302517049447 2315 72-73 -7.457212650899795 2315 74-75 -8.092937890090305 2315 76-77 -7.9354522144324005 2315 78-79 -7.631713213832082 2315 80-81 -8.438097655854907 2315 82-83 -7.680143477025439 2315 84-85 -6.9606974402072055 2315 86-87 -7.205623353485272 2315 88-89 -6.5142884994676855 2315 90-91 -6.398079986634787 2315 92-93 -6.132958965934636 2315 94-95 -6.371470615357536 2315 96-97 -6.374838662513904 2315 98-99 -6.23755855583536 2315 100-101 -5.942575902160887 2316 1 -1.265748141666922 2316 2 1.4850985994440293 2316 3 1.5341309657151463 2316 4 1.7047685140692899 2316 5 -1.7752691684399906 2316 6 -2.7950460311625918 2316 7 -3.1044216543708316 2316 8 -4.151500568592937 2316 9 -3.5016538548669303 2316 10-11 -3.1042152536176815 2316 12-13 -3.470538793634553 2316 14-15 -2.2228779326639625 2316 16-17 -2.2533047721091037 2316 18-19 -4.439444251998381 2316 20-21 -2.041042580329652 2316 22-23 -2.384672460474434 2316 24-25 -2.727284949187151 2316 26-27 -2.846845272327087 2316 28-29 -1.929740348108112 2316 30-31 0.9044485887737608 2316 32-33 -1.0790466360442856 2316 34-35 -7.474402681284442 2316 36-37 -2.8260468080072982 2316 38-39 -0.5918878310828184 2316 40-41 -0.749243762781056 2316 42-43 -1.0269393481724478 2316 44-45 -0.08402687617663318 2316 46-47 1.1489755707686342 2316 48-49 1.5626378235855007 2316 50-51 1.4511739689857848 2316 52-53 1.2498303198399654 2316 54-55 -0.5238027148048552 2316 56-57 0.5448407147241987 2316 58-59 -3.2337710680444083 2316 60-61 -3.3352011136359216 2316 62-63 -4.647315543595614 2316 64-65 -4.779525189589176 2316 66-67 -2.3334677322273976 2316 68-69 -2.2158579330976806 2316 70-71 -3.1530867785848926 2316 72-73 -2.7945498920173613 2316 74-75 -2.657406407455799 2316 76-77 -2.5302077133198466 2316 78-79 -2.9983414724205666 2316 80-81 -2.950865344449319 2316 82-83 -4.025974939567021 2316 84-85 -3.220840025546316 2316 86-87 -2.803379147565053 2316 88-89 -4.324572231393358 2316 90-91 -4.293402676506712 2316 92-93 -4.777187707745529 2316 94-95 -6.393211902842621 2316 96-97 -6.314832758667224 2316 98-99 -6.2437681374312355 2316 100-101 -6.015240968008623 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15973.0 3 5572.0 4 4919.0 5 5156.0 6 6492.0 7 8143.0 8 10383.0 9 12734.0 10 15485.0 11 18625.0 12 22253.0 13 25902.0 14 28952.0 15 33118.0 16 36548.0 17 36612.0 18 32443.0 19 31950.0 20 34390.0 21 37607.0 22 41096.0 23 46135.0 24 51004.0 25 56583.0 26 62780.0 27 69414.0 28 76637.0 29 83812.0 30 91751.0 31 100173.0 32 108070.0 33 116298.0 34 120816.0 35 115938.0 36 98650.0 37 60058.0 38 11801.0 39 110.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.89195754381394 19.331145163101134 14.884940950022903 19.891956343062027 2 15.844283203197604 22.25076101816826 38.68385602993683 23.221099748697316 3 21.177232898388475 26.849505352831628 29.814128168719716 22.159133580060185 4 12.26210320483814 17.546113678636775 38.18917513601165 32.00260798051343 5 12.51897772914038 39.47529358092208 32.93754168748033 15.068187002457215 6 29.003120352581952 37.05936633816497 17.575254628734598 16.362258680518487 7 26.746559511917273 35.488027903632286 20.464202592189267 17.301209992261175 8 26.865760366136946 31.532909905724356 19.72712870487134 21.874201023267357 9 27.206967835167205 17.733941533805567 21.356654866623344 33.70243576440388 10-11 25.212231924513695 27.00638044581019 25.890242257551854 21.891145372124253 12-13 27.94245703462869 25.36640364058774 26.620717406501733 20.07042191828184 14-15 24.00054232228661 26.034077075816768 28.004601627778026 21.960778974118597 16-17 20.736856623163185 29.734235565137507 27.87762394338238 21.651283868316938 18-19 20.35483367387955 27.942776692961008 31.628380345640224 20.074009287519214 20-21 22.470612921222973 25.515809002965046 31.282594785180724 20.73098329063126 22-23 21.415991536151054 26.029482022259458 31.764490227747395 20.79003621384209 24-25 21.78321555168179 26.157140489769194 31.9159212807838 20.143722677765222 26-27 21.130644953615143 25.811799947226294 33.02649713642003 20.031057962738526 28-29 20.10170344282326 27.64573281428601 32.131500360535405 20.121063382355327 30-31 20.442903636620915 26.4156773506063 31.959668825787002 21.18175018698579 32-33 20.56939209964275 26.672880190725163 32.001698902210045 20.75602880742204 34-35 21.806510393661778 26.40292638032205 31.55330724741609 20.23725597860008 36-37 21.36347812431984 25.889497837632184 32.466808188073756 20.280215849974223 38-39 20.77942447637907 26.917806772251772 32.06573907114855 20.2370296802206 40-41 20.665798869169425 27.218151014081986 31.768863772148322 20.347186344600267 42-43 20.445512748356126 27.37180497373208 30.560322707827154 21.622359570084644 44-45 20.846787449924996 26.661563555240747 30.80841886384766 21.683230130986594 46-47 21.74005167212736 27.511972633979475 30.416725603683442 20.331250090209718 48-49 21.49116665516623 27.47773510907754 30.00305747975322 21.028040756003012 50-51 20.307764983352232 28.005383418412944 30.813891206091892 20.872960392142932 52-53 21.170021576041613 28.750313705999215 29.018086504591732 21.061578213367444 54-55 20.418342398602697 27.738915621662613 29.70116089741257 22.14158108232212 56-57 21.46561577822439 27.784140761846963 29.11318800707586 21.637055452852792 58-59 22.331373385020008 27.395129679028997 29.009640590184134 21.263856345766865 60-61 21.213090227351962 27.76002211873466 30.490750767113735 20.536136886799643 62-63 20.854409719756937 27.965861016203636 30.121641271100195 21.05808799293923 64-65 20.50079886773198 28.885504475737278 29.412099151953413 21.201597504577325 66-67 21.03818505203537 28.052548782166593 28.649856697470483 22.259409468327558 68-69 20.783476486441796 27.957408162540897 29.29128172895364 21.967833622063672 70-71 21.55329493583018 27.954546438513727 28.923162180143073 21.56899644551302 72-73 21.295347436838195 27.474353371256495 29.815212640847893 21.415086551057417 74-75 20.4969794040566 29.375158420340625 28.702532358166522 21.425329817436257 76-77 20.91515780464691 28.046988830580684 28.67579924497705 22.362054119795356 78-79 20.988788676985344 28.331099307962276 28.874484350175827 21.80562766487656 80-81 21.426475896221646 28.328631448247982 28.562493683496236 21.682398972034132 82-83 21.10115239093979 28.063976291560223 29.18746506331336 21.647406254186627 84-85 20.741424416674413 28.607969291937348 28.71083183259302 21.939774458795224 86-87 20.79361156804814 28.729207600942985 28.522156907011375 21.955023923997498 88-89 20.619545668434377 28.588766133851422 27.765842280145154 23.025845917569047 90-91 20.793657942555637 27.93254331885763 28.323624158206144 22.950174580380594 92-93 21.507888486147372 28.491649624888428 28.36962433415819 21.63083755480601 94-95 20.885710910719293 27.997922851492834 28.501527391529496 22.614838846258376 96-97 20.129146372917443 28.0857402059283 29.05469903730873 22.730414383845527 98-99 20.689177036984184 28.531081198582324 27.594842221633563 23.18489954279993 100-101 20.427973403572928 27.848465550738588 28.29164302167403 23.43191802401445 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7013.0 1 4908.0 2 2855.0 3 2902.5 4 2628.0 5 2543.0 6 3456.5 7 5651.0 8 8351.0 9 9526.0 10 8232.0 11 6412.0 12 5605.0 13 5296.0 14 5190.5 15 5011.0 16 4934.0 17 5073.0 18 5229.5 19 5447.0 20 5738.5 21 6259.0 22 6966.5 23 7828.5 24 8832.0 25 10230.5 26 12110.5 27 14251.5 28 16506.0 29 18849.0 30 21596.0 31 24749.5 32 28035.5 33 31080.0 34 34315.0 35 36838.5 36 39938.0 37 43142.0 38 44523.0 39 45399.5 40 45878.0 41 47127.5 42 48801.5 43 50316.5 44 52201.0 45 54683.5 46 58651.5 47 68903.0 48 85550.5 49 85432.5 50 68410.5 51 56359.0 52 50517.5 53 48069.0 54 46230.5 55 43316.0 56 40288.0 57 37314.0 58 34021.5 59 30225.0 60 25900.5 61 21655.5 62 17582.5 63 13585.0 64 10326.0 65 7995.5 66 6154.5 67 4669.0 68 3429.0 69 2493.5 70 1873.5 71 1462.5 72 1145.5 73 904.5 74 734.0 75 613.0 76 483.0 77 334.0 78 235.5 79 181.0 80 121.5 81 84.0 82 57.5 83 37.0 84 24.5 85 14.0 86 9.5 87 8.0 88 6.0 89 4.0 90 4.5 91 4.0 92 2.0 93 2.0 94 1.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.96452225373519 2 2.2840399150591306 3 0.9625324971474005 4 0.8674554582234719 5 0.15844251240931215 6 0.0902914754122936 7 0.09006084584546781 8 0.0979022511175444 9 0.0934626319561481 10-11 1.6820390882521334 12-13 1.276073393247051 14-15 0.48847342253700593 16-17 0.08619780060113597 18-19 0.09383740500224 20-21 0.11713099125164395 22-23 0.13321740353774222 24-25 0.14448942361635234 26-27 0.14183718359785583 28-29 0.5287759393398113 30-31 0.17112713858473014 32-33 0.15432000890230127 34-35 0.13918494357935934 36-37 0.12995976090632808 38-39 0.37952978090767725 40-41 0.1169291903806714 42-43 0.11773639386456164 44-45 0.12145529562962737 46-47 0.1330732600584761 48-49 0.14786238103117938 50-51 0.15968214633100072 52-53 0.1766910768844021 54-55 0.17585504470465865 56-57 0.14866958451506962 58-59 0.13143002439484244 60-61 0.11041390511784305 62-63 0.11496883906265225 64-65 0.11133642338514618 66-67 0.10721391987813533 68-69 0.10363916159233573 70-71 0.1190913425696631 72-73 0.13168948265752145 74-75 0.14068403576372693 76-77 0.1602875489439184 78-79 0.16317041852924066 80-81 0.16190195591169887 82-83 0.1544641523815674 84-85 0.14183718359785583 86-87 0.14068403576372693 88-89 0.13768585139499176 90-91 0.14506599753341679 92-93 0.1363597313857435 94-95 0.18239915866334022 96-97 0.1871847221749752 98-99 0.20304050489424771 100-101 0.22630526244779842 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1734383.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.05181390563172 #Duplication Level Percentage of deduplicated Percentage of total 1 78.77269307585134 43.36579640057213 2 11.352842104688552 12.499891016946691 3 4.179992317895059 6.903484776351869 4 1.9071351440067343 4.199649961629956 5 1.054836855725008 2.9035341141087425 6 0.6298825387778808 2.080570578432405 7 0.4244221400038814 1.6355646068246559 8 0.29857112501317434 1.3149505609456302 9 0.21425377122152534 1.0615552867680484 >10 1.0535467681508683 10.362993009512952 >50 0.07226169429058579 2.710865785882747 >100 0.03467414582947318 3.4304848781604536 >500 0.0013641819198037088 0.49873116007919444 >1k 0.0027283638396074176 3.0964014149928754 >5k 4.5472730660123625E-4 1.7358272626372864 >10k+ 3.410454799509272E-4 2.1996991861542883 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13926 0.8029368369039594 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10857 0.6259863017568784 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10365 0.5976188650373072 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7879 0.4542825892550838 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7456 0.4298935125632574 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6945 0.4004305854012637 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5456 0.31457872915036644 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4547 0.2621681600892075 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3617 0.20854678580221322 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3374 0.194536039617547 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3239 0.18675229173717686 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2938 0.1693974168335368 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2757 0.15896142893467013 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2525 0.1455849140587748 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2309 0.13313091745018257 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2138 0.1232715034683804 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2103 0.1212534947586548 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2090 0.12050394866647103 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1845 0.10637788769839186 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1826 0.10528239725596941 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.7657391706445465E-5 0.0 18-19 0.0 0.0 0.0 1.1531478341289092E-4 0.0 20-21 0.0 0.0 0.0 3.1711565438545004E-4 0.0 22-23 0.0 0.0 0.0 8.936895714499047E-4 0.0 24-25 0.0 0.0 0.0 0.0017873791428998091 0.0 26-27 0.0 0.0 0.0 0.0028540408894690505 0.0 28-29 0.0 0.0 0.0 0.004583762640662414 0.0 30-31 0.0 0.0 0.0 0.008763923539379711 0.0 32-33 0.0 0.0 0.0 0.016547671419749846 0.0 34-35 0.0 0.0 0.0 0.02842509411127761 0.0 36-37 0.0 0.0 0.0 0.04554933944809192 0.0 38-39 0.0 0.0 0.0 0.07097624919063436 0.0 40-41 0.0 0.0 0.0 0.10447519377207917 0.0 42-43 0.0 0.0 0.0 0.1371092774779273 0.0 44-45 0.0 0.0 0.0 0.17954511777387117 0.0 46-47 0.0 0.0 0.0 0.23207100161844296 0.0 48-49 0.0 0.0 0.0 0.28312662197450045 0.0 50-51 0.0 0.0 0.0 0.3431479667409102 0.0 52-53 0.0 0.0 0.0 0.4010071593183282 0.0 54-55 0.0 0.0 0.0 0.4672266736931808 0.0 56-57 0.0 0.0 0.0 0.5512911508011782 0.0 58-59 0.0 0.0 0.0 0.6415537975176187 0.0 60-61 0.0 0.0 0.0 0.7412145990822097 0.0 62-63 0.0 0.0 0.0 0.845833936333555 0.0 64-65 0.0 0.0 0.0 0.9512604770687905 0.0 66-67 0.0 0.0 0.0 1.0766076466386028 0.0 68-69 0.0 0.0 0.0 1.2269781242090128 5.7657391706445465E-5 70-71 0.0 0.0 0.0 1.3910998897014095 5.7657391706445465E-5 72-73 0.0 0.0 0.0 1.5581621821708354 5.7657391706445465E-5 74-75 0.0 0.0 0.0 1.7305289546772542 5.7657391706445465E-5 76-77 0.0 0.0 0.0 1.9129857707323008 5.7657391706445465E-5 78-79 0.0 0.0 0.0 2.1374171679496397 5.7657391706445465E-5 80-81 0.0 0.0 0.0 2.386583586209044 5.7657391706445465E-5 82-83 0.0 0.0 0.0 2.639151790579128 5.7657391706445465E-5 84-85 0.0 0.0 0.0 2.883215529672512 5.7657391706445465E-5 86-87 0.0 0.0 0.0 3.1358702201301556 5.7657391706445465E-5 88-89 0.0 0.0 0.0 3.4204671055931706 5.7657391706445465E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12275 0.0 26.161798 1 TACATGG 12470 0.0 25.246157 2 ACATGGG 13015 0.0 23.578636 3 GAGTACT 11110 0.0 23.476257 12-13 GTATCGA 175 2.551331E-6 22.902506 1 AGTACTT 11635 0.0 22.416952 12-13 CATGGGA 8550 0.0 21.915245 4 CATGGGG 3960 0.0 21.792313 4 GTACTTT 12295 0.0 21.616552 14-15 CGACGGT 140 4.249749E-4 20.761705 7 GTGTAAG 880 0.0 19.92583 1 ACTTTTT 14785 0.0 19.684845 16-17 AGAGTAC 19510 0.0 19.602097 10-11 CTATACC 270 2.2038876E-8 19.424454 4 ATGGGAG 3105 0.0 19.360703 5 TACTTTT 13775 0.0 19.242365 14-15 AGTACAT 9395 0.0 18.28723 2 ATGGGAT 3390 0.0 18.01905 5 TAATGCG 135 0.008397502 17.941153 9 GTATCAA 33195 0.0 17.235525 1 >>END_MODULE